Mercury-Based Consumption Advice for Northern Pike from Mille Lacs

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To: Neil Kmiecik, Biological Services Director From: Sara Moses, Environmental Biologist Date: April 28, 2015 Re: Mercury-Based Consumption Advice for Northern Pike from Mille Lacs Under a recent Great Lakes Restoration Initiative (GLRI) grant (GL-00E00613-0, Mercury Testing and Updating Tribal Walleye Consumption Advice), GLIFWC collected and tested for mercury 28 northern pike from Mille Lacs in 2013 and 2014. Skin-off fillets were tested for total mercury by the Lake Superior Research Institute (LSRI) at the University of Wisconsin Superior. In addition, GLIFWC s mercury database contains mercury data from the Minnesota Department of Health for 44 northern pike skin-on fillets from Mille Lacs collected between 1985 and 2008. This memo describes the utilization of this data for developing mercury-based consumption advice for northern pike from Mille Lacs. Available information on the length, weight, age, sex, and mercury concentration of all 72 northern pike in the database from Mille Lacs are shown in Appendix A. Mercury concentrations ranged from 0.011 to 0.607 µg/g (ppm), with an average of 0.159 µg/g. A plot of mercury concentration versus walleye length is shown in Figure 1, below. The mean length of northern pike harvested by GLIFWC tribes from Mille Lacs during spring spearing and netting is typically ~26 inches.

Figure 1: Mercury concentration versus northern pike length for Mille Lacs. [Note: The 75% confidence and prediction upper bounds shown are used in the determination of northern pike consumption advice.] An analysis similar to that used to assign color codes to inland lakes for the walleye Mercury Maps was performed for the Mille Lacs northern pike. The major modification was that fish mercury was normalized to a 30- or 36-inch northern pike, rather than the 20-inch length applied to walleye. Two methods were used for determining the appropriate length for normalization and subsequent consumption advice. Both methods, which resulted in identical consumption advice recommendations, are detailed below. Method 1 GLIFWC s walleye consumption advice, as communicated by our Mercury Maps, is standardized to a 20-inch walleye. 77.6-85.8% of walleye harvested by GLIFWC member tribes from Mille Lacs from the 2008-2009 through 2010-2011 quota years were less than 20-inches, and thus covered by our walleye consumption advice. For Method 1, a length of 30 inches was chosen for developing consumption for northern pike from Mille Lacs because it covers approximately the same percentage of northern pike in Mille Lacs. In other words, approximately the same percentage of northern pike (79.8-84.8%) harvested from Mille Lacs in 2

recent years are <30 inches as walleye <20 inches, as shown in Table 1 below. A length of 30 inches allows the consumption advice developed to be applicable to the majority of the northern pike harvested and is consistent with the length used by the Minnesota Department of Health for issuing statewide northern pike consumption advice. Table 1: Percent of walleye <20 inches and northern pike <30 inches and average walleye and northern pike length (all gear types) harvested by GLIFWC tribes from Mille Lacs by quota year Quota Year % Walleye <20 inches Average Walleye Length (inches) # of Walleye Measured % Northern Pike <30 inches Average Northern Pike Length (inches) # of Walleye Measured 2008-2009 84.1% 17.9 7,930 79.8% 27.0 1,599 2009-2010 85.8% 17.5 10,832 80.1% 26.4 1,953 2010-2011 77.6% 18.1 8,050 84.8% 25.7 1,951 The result based on the available information is that 30-inch northern pike from Mille Lacs fall into the blue consumption advice category for the general population and the yellow category for the sensitive population. This corresponds to the following consumption advice for northern pike (up to 30 inches) from Mille Lacs: MILLE LACS NORTHERN PIKE (up to 30 ) CONSUMPTION ADVICE Sensitive Population General Population (women of childbearing age and children (women beyond childbearing age and under 15) men 15 and older) Eat up to 2 meals or 16 oz. per Eat up to 8 meals or 64 oz. per These are the same consumption advice categories as GLIFWC recommends for walleye (up to 20 inches) from Mille Lacs. Method 2 The second method for choosing the length northern pike for which advice should be generated was based on the method originally used to choose the 20-inch walleye length. 20 inches was chosen for walleye because it is protective of the vast majority of walleye harvested by GLIFWC member tribes in Wisconsin, where the majority of the walleye harvest lakes are located. Between 1985 and 2013, 94.1% of the 618,668 walleye harvested in Wisconsin were under 20 inches. In order to develop consumption advice protective of approximately the same percentage 3

(93.9%) of the 926 tribally harvested northern pike from 1985 to 2013, a length of 36 inches was used to develop consumption advice. The result based on the available information is that 36-inch northern pike from Mille Lacs fall into the blue consumption advice category for the general population and the yellow category for the sensitive population. This corresponds to the following consumption advice for northern pike (up to 36 inches) from Mille Lacs: MILLE LACS NORTHERN PIKE (up to 36 ) CONSUMPTION ADVICE Sensitive Population General Population (women of childbearing age and children (women beyond childbearing age and under 15) men 15 and older) Eat up to 2 meals or 16 oz. per Eat up to 8 meals or 64 oz. per These are the same consumption advice categories as GLIFWC recommends for walleye (up to 20 inches) from Mille Lacs and is identical to the advice generated for a 30-inch pike. Mercury is fairly low in Mille Lacs fish relative to other inland lakes across the ceded territories. Cc: John Coleman, Environmental Section Leader 4

APPENDIX A Length, weight, age, sex, and mercury concentration of northern pike collected from the Mille Lacs used for generating consumption advice 5

Fish ID Collection Date Agency Length (inches) Weight (Pounds) Age (Years) Sex Form Mercury (µg/g, ppm) 12909 5/15/2013 GLIFWC 19.9 1.99 ----- F Skin off fillet 0.053 11744 5/15/2013 GLIFWC 21.0 1.97 3 F Skin off fillet 0.146 12789 5/15/2013 GLIFWC 22.2 2.36 2 F Skin off fillet 0.109 12910 5/15/2013 GLIFWC 24.5 3.10 3 M Skin off fillet 0.145 11976 5/15/2013 GLIFWC 26.5 4.43 3 F Skin off fillet 0.166 12911 5/15/2013 GLIFWC 27.9 4.84 4 F Skin off fillet 0.187 11996 5/15/2013 GLIFWC 32.0 5.46 5 F Skin off fillet 0.211 11281 5/15/2013 GLIFWC 33.3 11.2 7 F Skin off fillet 0.169 12908 5/15/2013 GLIFWC 33.5 8.78 5 F Skin off fillet 0.156 11743 5/15/2013 GLIFWC 39.3 13.9 9 F Skin off fillet 0.197 11977 5/30/2015 GLIFWC 39.3 13.8 8 U Skin off fillet 0.292 12645 6/3/2013 GLIFWC 35.4 3.62 5 U Skin off fillet 0.165 11913 6/3/2013 GLIFWC 37.1 11.9 6 U Skin off fillet 0.200 11282 6/6/2013 GLIFWC 24.2 2.94 3 M Skin off fillet 0.089 11978 6/6/2013 GLIFWC 24.3 3.27 4 M Skin off fillet 0.077 11280 6/6/2013 GLIFWC 25.2 3.24 4 M Skin off fillet 0.097 6854 4/29/2014 GLIFWC 22.4 2.90 4 F Skin off fillet 0.152 12820 4/29/2014 GLIFWC 23.9 3.30 4 M Skin off fillet 0.185 6849 4/29/2014 GLIFWC 25.1 4.60 5 F Skin off fillet 0.186 12898 4/29/2014 GLIFWC 26.0 4.25 4 F Skin off fillet 0.221 12884 4/29/2014 GLIFWC 27.5 6.07 5 F Skin off fillet 0.168 6846 4/29/2014 GLIFWC 30.0 7.99 7 F Skin off fillet 0.216 12883 4/29/2014 GLIFWC 32.4 10.2 8 F Skin off fillet 0.246 12897 4/29/2014 GLIFWC 35.0 11.5 9 F Skin off fillet 0.249 12891 4/29/2014 GLIFWC 37.0 10.1 13 F Skin off fillet 0.594 12910 4/29/2014 GLIFWC 41.0 17.7 13 F Skin off fillet 0.375 12986 4/30/2014 GLIFWC 30.0 5.87 4 F Skin off fillet 0.143 13000 4/30/2014 GLIFWC 30.6 5.95 5 M Skin off fillet 0.172 ----- 9/26/1985 MDNR 18.9 1.5 ----- ---- Skin on fillet 0.11 ----- 9/26/1985 MDNR 22.8 2.8 ----- ---- Skin on fillet 0.12 ----- 9/26/1985 MDNR 25.3 3.8 ----- ---- Skin on fillet 0.12 ----- 9/26/1985 MDNR 25.4 3.5 ----- ---- Skin on fillet 0.16 90438 10/3/1990 MDNR 19.7 1.9 ----- ---- Skin on fillet 0.025 90439 10/3/1990 MDNR 24.1 3.3 ----- ---- Skin on fillet 0.063 90440 10/3/1990 MDNR 26.6 3.5 ----- ---- Skin on fillet 0.100 ----- 10/1/1997 MDNR 21.7 2.34 ----- ---- Skin on fillet 0.097 ----- 10/1/1997 MDNR 24.8 3.22 ----- ---- Skin on fillet 0.10 6

CONTINUED. Fish Collection ID Date Agency Length (inches) Weight (Pounds) Age (Years) Sex Form Mercury (µg/g, ppm) ----- 10/1/1997 MDNR 25.5 3.51 ----- ---- Skin on fillet 0.23 ----- 10/1/1997 MDNR 27.6 5.42 ----- ---- Skin on fillet 0.10 ----- 10/1/1997 MDNR 28.3 5.18 ----- ---- Skin on fillet 0.13 ----- 10/1/1997 MDNR 29.2 6.66 ----- ---- Skin on fillet 0.14 ----- 10/1/1997 MDNR 32.0 9.10 ----- ---- Skin on fillet 0.32 ----- 10/1/1997 MDNR 32.7 9.55 ----- ---- Skin on fillet 0.24 ----- 10/1/1997 MDNR 33.8 10.7 ----- ---- Skin on fillet 0.12 ----- 10/1/1997 MDNR 35.3 10.1 ----- ---- Skin on fillet 0.20 30845 9/24/2003 MDNR 20.3 2.26 ----- ---- Skin on fillet 0.050 30846 9/24/2003 MDNR 26.5 4.70 ----- ---- Skin on fillet 0.092 30847 9/24/2003 MDNR 31.2 7.54 ----- ---- Skin on fillet 0.146 30848 9/24/2003 MDNR 32.0 8.82 ----- ---- Skin on fillet 0.172 30849 9/24/2003 MDNR 33.7 9.35 ----- ---- Skin on fillet 0.161 081220 9/23/2008 MDNR 15.1 0.84 ----- ---- Skin on fillet 0.011 081221 9/23/2008 MDNR 18.2 1.55 ----- ---- Skin on fillet 0.041 081223 9/23/2008 MDNR 18.8 1.59 ----- ---- Skin on fillet 0.016 081224 9/23/2008 MDNR 19.0 1.74 ----- ---- Skin on fillet 0.043 081222 9/23/2008 MDNR 19.5 1.65 ----- ---- Skin on fillet 0.032 081225 9/23/2008 MDNR 21.1 1.90 ----- ---- Skin on fillet 0.072 081226 9/23/2008 MDNR 21.5 2.20 ----- ---- Skin on fillet 0.051 081228 9/23/2008 MDNR 21.6 2.62 ----- ---- Skin on fillet 0.058 081229 9/23/2008 MDNR 22.0 2.67 ----- ---- Skin on fillet 0.072 081227 9/23/2008 MDNR 22.2 2.62 ----- ---- Skin on fillet 0.065 081230 9/23/2008 MDNR 23.8 3.24 ----- ---- Skin on fillet 0.110 081231 9/23/2008 MDNR 24.5 3.48 ----- ---- Skin on fillet 0.083 081233 9/23/2008 MDNR 25.0 3.59 ----- ---- Skin on fillet 0.095 081232 9/23/2008 MDNR 25.7 4.08 ----- ---- Skin on fillet 0.161 081235 9/23/2008 MDNR 25.9 3.92 ----- ---- Skin on fillet 0.292 081236 9/23/2008 MDNR 27.5 3.88 ----- ---- Skin on fillet 0.174 081234 9/23/2008 MDNR 27.8 3.99 ----- ---- Skin on fillet 0.138 081237 9/23/2008 MDNR 29.1 4.01 ----- ---- Skin on fillet 0.179 081238 9/23/2008 MDNR 29.2 4.37 ----- ---- Skin on fillet 0.145 081239 9/23/2008 MDNR 29.3 5.20 ----- ---- Skin on fillet 0.189 081240 9/23/2008 MDNR 36.2 8.47 ----- ---- Skin on fillet 0.458 081241 9/23/2008 MDNR 38.3 11.8 ----- ---- Skin on fillet 0.607 7