Nutrition Spotlight: U.S. Dairy Proteins for Healthy and Active Lifestyles. U.S. Dairy Proteins: Smart Protein Choice DID YOU KNOW

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Nutrition Spotlight: U.S. Dairy Proteins for Healthy and Active Lifestyles U.S. whey s are well-known as a key component of training regimens for professional athletes and gym enthusiasts. Today, broader consumer groups including sports enthusiasts, seniors as well as health-minded and weight-conscious adults also are discovering the benefits of diets as part of an overall healthy, active lifestyle. Incorporating high-quality U.S. milk and whey s into foods and beverages regularly consumed throughout the day can help consumers attain higher- diets while maintaining a healthy weight, curbing hunger and enhancing exercise recovery. U.S. Dairy Proteins: Smart Protein Choice Dietary guidance around the world tends to focus on the amount of required to prevent nutritional deficiencies. However, beyond quantity, the benefits of s for human health can be optimized by also paying attention to quality as well as intake timing. DID YOU KNOW nnaturally found in dairy, U.S. whey and milk s are high quality and complete with all of the essential and nonessential amino acids the body needs. nresearch demonstrates that U.S. whey may benefit weight management, body composition, muscle health, post-exercise recovery, healthy aging and more. nthere are three important factors when choosing : amount, quality and timing. Aim for 20 to 30 grams of high-quality at each meal.

U.S. DAIRY PROTEINS FOR HEALTHY AND ACTIVE LIFESTYLES High-quality U.S. dairy s are an optimum choice to fuel active lifestyles and help people reach their goals faster: Curb hunger Calorie for calorie, whey can help people feel fuller longer than carbohydrates or fats. 10-13 Get lean Consuming whey and performing regular resistance exercise can help build more lean muscle compared with resistance training alone or resistance training combined with carbohydrate consumption. 14-19 Enhance exercise recovery Consuming whey in proximity to exercise helps to build and repair muscle. 14,26,27 Help maintain muscle Consuming more high-quality and engaging in regular exercise can help people maintain muscle mass as they age, which may allow for a more active lifestyle. 20,21 Maintain a healthy weight A reduced-calorie, higher- diet including whey may improve the quality of weight loss by helping people lose more fat and/or maintain more lean muscle. 1-9 2

Fitting in More Dairy Protein: Balancing Timing and Quantity The key to optimizing the benefits of U.S. whey and milk s is to understand how much and when they should be consumed. Recent research indicates that people should balance their intake throughout the day to maximize health and wellness benefits; 22-25 there is an upper limit to the amount of that can be absorbed or used by the body at one time. Aiming for 20 to 30 grams of high-quality per meal 22,23,24 is a good rule of thumb to ensure the benefits of a are maximized. Attention also should be paid to intakes after exercise; research shows that consuming 20 grams of whey post-exercise can increase muscle synthesis (MPS) in healthy adults. 26,27 (A) ADEQUATE PROTEIN DISTRIBUTION (B) INADEQUATE PROTEIN DISTRIBUTION MAXIMAL PROTEIN SYNTHESIS BREAKFAST LUNCH DINNER BREAKFAST LUNCH DINNER ~30g ~30g ~30g ~10g ~20g ~60g Source: Paddon-Jones D, Rasmussen BB. Dietary recommendations and the prevention of sarcopenia: Protein, amino acid metabolism and therapy. Curr Opin Clin Nutr Metab Care. 2009;12(1):86-90. 3

U.S. DAIRY PROTEINS FOR HEALTHY AND ACTIVE LIFESTYLES There are easy ways to add more to meals throughout the day. BREAKFAST LUNCH Enjoy Greek yogurt and top with nuts and fruit. Add a fruit smoothie that contains whey. SNACK DINNER Grab a nutrition/energy bar made with whey or milk. Stir whey into soups to complement the meal. 4

THE RESEARCH Achieving Muscle Gains and Hunger Control With High-quality Dairy Protein The International Olympic Committee Consensus Statement on Sports Nutrition states, Foods or snacks that contain high-quality s should be consumed regularly throughout the day as part of the day s total intake, and in particular soon after exercise, in quantities sufficient to maximize the synthesis of s, to aid in long-term maintenance or gain of muscle and bone and in the repair of damaged tissues. Ingestion of foods or drinks providing 15-25 g of such after each training session will maximize the synthesis of s that underpins these goals. 28 A Closer Look at the Science MAXIMIZING MUSCLE PROTEIN SYNTHESIS Research shows that consuming 20 grams of whey at rest and after exercise can maximize MPS..12.10 RESTED MYOFIBRILLAR FSR (%-H- 1 ).08.06.04 EXERCISED, Means findings were significantly different from each other (P < 0.05; n =12). FSR = fractional synthesis rate..02 0 0 10 20 40 DOSE OF WHEY PROTEIN (G) Forty-eight resistance-trained young men consumed a high- breakfast and, three hours later, performed an intense, one-legged resistance exercise session. Immediately after exercise, subjects consumed a beverage containing 0, 10, 20 or 40 grams of whey isolate. After measurements were made, it was shown that 20 grams of whey maximized MPS at rest and following resistance exercise. 26 5

U.S. DAIRY PROTEINS FOR HEALTHY AND ACTIVE LIFESTYLES CONTROLLING HUNGER Higher- diets have been shown to improve perceptions of fullness and reduce the desire to eat when calories are restricted, which may help achieve better results when trying to lose weight. FULLNESS DESIRE TO EAT FULLNESS (MM) HP-AM 100 NP-AM 100 90 Area Under the Curve: 90 80 30% *p<0.123 80 70 70 60 60 50 50 40 40 30 30 18% 20 20 10 10 DESIRE TO EAT (MM) HP-AM NP-AM Area Under the Curve: *p<0.05 18% 30% 0 0 0 60 120 180 240 0 60 120 180 240 TIME (MIN) TIME (MIN) Thirty-eight women consumed a 750-kilocalorie per day energy-deficit diet with a content of 30 percent (higher- diet) or 18 percent (normal- diet) for nine weeks. On separate days, metabolic, appetite and hormonal responses were measured over four hours when the women consumed a higher- diet or a normal- diet. Results showed that higher- diets improved perceptions of satiety and pleasure when consuming fewer calories. 29 GET IN TOUCH Looking for more information on dairy s? While the U.S. Dairy Export Council (USDEC) does not manufacture or sell dairy products, we proudly support the people who do. Search ThinkUSAdairy.org/Nutrition for more information on the health and nutritional benefits of U.S. dairy s. 6

1 Layman DK, Evans E, Baum JI, et al. Dietary and exercise have additive effects on body composition during weight loss in adult women. J Nutr. 2005;135(8):1903-1910. 2 Pasiakos SM, Cao JJ, Margolis LM, et al. Effects of high- diets on fat-free mass and muscle synthesis following weight loss: a randomized controlled trial. FASEB J. 2013;27(9):3837-3847. 3 Krieger JW, Sitren HS, Daniels MJ, Langkamp-Henken B. Effects of variation in and carbohydrate intake on body mass and composition during energy restriction: a meta-regression. Am J Clin Nutr. 2006;83(2):260-274. 4 Claessens M, van Baak MA, Monsheimer S, Saris WH. The effect of a low-fat, high- or high-carbohydrate ad libitum diet on weight loss maintenance and metabolic risk factors. Int J Obes (Lond). 2009;33(3):296-304. 5 Westerterp-Plantenga MS, Lejeune MP, Nijs I, van Ooijen M, Kovacs EM. High intake sustains weight maintenance after body weight loss in humans. Int J Obes Relat Metab Disord. 2004;28(1):57-64. 6 Lejeune MP, Kovacs EM, Westerterp-Plantenga MS. Additional intake limits weight regain after weight loss in humans. Br J Nutr. 2005;93(2):281-289. 7 Layman DK, Walker DA. Potential importance of leucine in treatment of obesity and the metabolic syndrome. J Nutr. 2006;136(Suppl 1):319S-323S. 8 Josse AR, Atkinson SA, Tarnopolsky MA, Phillips SM. Increased consumption of dairy foods and during diet- and exercise-induced weight loss promotes fat mass loss and lean mass gain in overweight and obese premenopausal women. J Nutr. 2011;141(9):1626-1634. 9 Gordon MM, Bopp MJ, Easter L, et al. Effects of dietary on the composition of weight loss in post-menopausal women. J Nutr Health Aging. 2008;12(8):505-509. 10 Institute of Medicine. Dietary Reference Intakes for Energy, Carbohydrate, Fiber, Fat, Fatty Acids, Cholesterol, Protein, and Amino Acids. Washington, DC: The National Academies Press; 2005: 843. 11 Smeets AJ, Soenen S, Luscombe-Marsh ND, Ueland O, Westerterp-Plantenga MS. Energy expenditure, satiety, and plasma ghrelin, glucagon-like peptide 1, and peptide tyrosinetyrosine concentrations following a single high- lunch. J Nutr. 2008;138(4):698-702. 12 Beasley JM, Ange BA, Anderson CAM, et al. Associations between macronutrient intake and self-reported appetite and fasting levels of appetite hormones: results from the Optimal Macronutrient Intake Trial to Prevent Heart Disease. Am J Epidemiol. 2009;169(7):893-900. 13 Leidy HG, Armstrong CL, Tang M, Mattes RD, Campbell WW. The influence of higher intake and greater eating frequency on appetite control in overweight and obese men. Obesity. 2010;18(9):1725-1732. 14 Howarth KR, Moreau NA, Phillips SM, Gibala MJ. Coingestion of with carbohydrate during recovery from endurance exercise stimulates skeletal muscle synthesis in humans. J Appl Physiol. 2009;106(4):1394-1402. 15 Tang JE, Manolakos JJ, Kujbida GW, et al. Minimal whey with carbohydrate stimulates muscle synthesis following resistance exercise in trained young men. Appl Physiol Nutr Metab. 2007;32(6):1132-1138. 16 Tang JE, Moore DR, Kujbida BW, Tamopolsky MA, Phillips SM. Ingestion of whey hydrolysate, casein, or soy isolate: effects on mixed muscle synthesis at rest and following resistance exercise in young men. J Appl Physiol. 2009;107(3):987-992. 17 Volek JS, Volk BM, Gomez AL, et al. Whey supplementation during resistance training augments lean body mass. J Am Coll Nutr. 2013;32(2):122-135. 18 Hulmi JJ, Kovanen V, Selanne H, et al. Acute and long-term effects of resistance exercise with or without ingestion on muscle hypertrophy and gene expression. Amino Acids. 2009;37(2):297-308. 19 Holm L, Olesen JL, Matsumoto K, et al. Protein-containing nutrient supplementation following strength training enhances the effect on muscle mass, strength, and bone formation in postmenopausal women. J Appl Physiol. 2008;105(1):274-281. 20 Yang Y, Breen L, Burd NA, et al. Resistance exercise enhances myofibrillar synthesis with graded intakes of whey in older men. Br J Nutr. 2012;108(10):1780-1788. 21 Yang Y, Churchward-Venne TA, Burd NA, et al. Myofibrillar synthesis following ingestion of soy isolate at rest and after resistance exercise in elderly men. Nutr Metab (Lond). 2012;9(1):57. 22 Paddon-Jones D, Rasmussen BB. Dietary recommendations and the prevention of sarcopenia: Protein, amino acid metabolism and therapy. Curr Opin Clin Nutr Metab Care. 2009;12(1):86-90. 23 Breen L, Phillips SM. Skeletal muscle metabolism in the elderly: Interventions to counteract the anabolic resistance of ageing. Nutr Metab (Lond). 2011;8:68. 24 Pennings B, Groen B, de Lange A, et al. Amino acid absorption and subsequent muscle accretion following graded intakes of whey in elderly men. Am J Physiol Endocrinol Metab. 2012;302(8):E992-9. 25 Layman, DK. Dietary Guidelines should reflect new understandings about adult needs. Nutr Metab (Lond). 2009;6:12. 26 Witard OC, Jackman SR, Breen L, et al. Myofibrillar muscle synthesis rates subsequent to a meal in response to increasing doses of whey at rest and after resistance exercise. Am J Clin Nutr. 2014;99(1):86-95. 27 Tipton KD, Elliott TA, Cree MG. Ingestion of casein and whey s result in muscle anabolism after resistance exercise. Med Sci Sports Exerc. 2004;36(12):2073-2081. 28 International Olympic Committee. IOC consensus statement on sports nutrition 2010. J Sports Sci. 2011;29(Suppl 1):S3-S4. 29 Leidy H, Carnell N, Mattes RD, et al. Higher intake preserves lean mass and satiety with weight loss in pre-obese and obese women. Obesity. 2007;15(2):421-429. 7

To learn more and find a USDEC representative near you, go to ThinkUSAdairy.org/global-presence. 2015 U.S. Dairy Export Council WW011E Managed by Dairy Management Inc.