Screening Varieties for resistance to Tomato Yellow Leaf Curl Virus Spring 2007

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Screening Varieties for resistance to Tomato Yellow Leaf Curl Virus Spring 2007 Monica Ozores-Hampton, Phil Stansly, Eric Simonne and Eugene McAvoy

Tomatoes in Florida Staked tomatoes (#1 USA), Fall/Winter/Spring-Mature Green 38,200 acres/u$622,251 (Millions) 70% of the tomato production is in the Southwest Florida area: Collier (14,086 acres); Manatee (11,300 acres) *

Facts: Tomato Yellow Leaf Curl Virus (TYLCV) Is the worst tomato disease, because can have tremendous impact in yields. The disease is induced by a number of begomoviruses, transmitted by the whitefly Bemisia tabaci (Gennadius) biotype B and other B. tabaci biotypes. Leaf symptoms include chlorotic margins, small leaves which are cupped, thick and rubbery. The majority (up to 90%) of flowers abscise after infection. In order to lessen the incidence of the TYLCV the tomato free periods is very important. Now if the virus become more strong another tool is resistant varieties. Ozores-Hampton et al., 2008. Evaluation of TYLC virus-resistant varieties under commercial conditions in Southwest Florida. Fla. Tomato Inst. Proc. PRO525, pp.12-17.

The TYLCV resistant genetic material is coming from Israel a dry weather conditions. Objectives To evaluate the horticultural performance of the TYLCV varieties such as yield, fruit qualities and post-harvest characteristics under South Florida field conditions.

Spring 2007 10 varieties SWFREC/IMK 2007

Tomato Varieties Evaluation Spring 2007 Variety Source Pruning No Suckers & Ground Suckers BHN 745 FLA 8576 FLA 8579 FLA 8580 HA 3074 (Inbar) HA 3075 (Ofri) HA 3078 Tygress FL 47 (control) HA 3371 (Shanty) HA 3811 BHN Seed 2-3 UFz 2-3 UF 2-3 UF 2-3 Hazera 2-3 Hazera 2-3 Hazera 2-3 Seminis 2-3 Seminis 2-3 Roma Tomatoes Hazera 2-3 Hazera 2-3

Three Harvest Extra-large (5/6), Large (6/6), Medium (6/7) and un-marketable

Results

Spring 2007 Whitefly pressure was heavier than other Springs. In 2007, the average whitefly count was 9.9 ± 0.38 (mean ± SE) adult per leaf Number of TYCLV symptomatic plants was high in 2007.

TYLCV Evaluation Spring 2007 Variety Virus incidence (% ) BHN 745 33.9cd FLA 8576 39.3c FLA 8579 32.1cd FLA 8580 62.5b HA 3074 (Inbar) HA 3075 (Ofri) HA 3078 21.4cd 35.7cd 28.6cd Tygress 16.1d FL 47 (control) 94.6a

Spring 2007 Round Tomatoes

First Harvest Marketable Fruit Yield SWFREC/Immokalee, Spring 2007 Varieties Yield (25-lb boxes/acre) First Harvest XL L M Total Round Tomatoes BHN 745 630cd 72abc 41 744 FLA 8576 442cd 39c 48 530 FLA 8579 694bc 64abc 39 797 FLA 8580 590cd 105a 36 731 HA 3074 (Inbar) 991ab 68abc 33 1,092 HA 3075 (Ofri) 726bc 96ab 56 878 HA 3078 1,118a 70abc 50 1,238 Tygress 722bc 59bc 53 835 Florida 47 (control) 330d 43c 59 431 Sig. ** * ns ns

Total Marketable Fruit Yield SWFREC/Immokalee, Spring 2007 Varieties Yield (25-lb boxes/acre) Total Harvest XL L M Cull Total Round Tomatoes BHN 745 770ab 128d 175 1,433a 1,073bcd FLA 8576 584ab 128d 273 1,095bc 985cd FLA 8579 888ab 175bcd 249 1,022bc 1,312bcd FLA 8580 813ab 305a 324 694d 1,442bc HA 3074 (Inbar) 1,472a 232abc 311 833cd 2,015a HA 3075 (Ofri) 1,025a 264ab 271 708d 1,561ab HA 3078 1,590a 217abcd 262 1,094bc 2,069a Tygress 1,039a 211bcd 269 1,146b 1,518abc Florida 47 (control) 430b 169cd 255 596d 854d Sig. ** ** ns ** **

Romas Spring 2007 First and Total marketable fruit yield SWFREC/Immokalee, Spring 2007 Roma Tomatoes Varieties First total harvest Total Culls Total Harvest Shanty 600 1,300 951 HA 3811 159 314 622 Sig. * * *

Unmarketable (cull) Fruit by Defect Category Spring 2007 Varieties Unmarketable fruits by cull category (%) Total Culls BES Zip+CatF SS+YS Crk OS Other Round Tomatoes BHN 745 133.4a 7.2ab 13.2ab 60.9a 30.5a 15.6a 6.0 FLA 8576 111.9a 6.6ab 30.3a 48.6a 11.8ab 8.5b 6.1 FLA 8579 77.9ab 7.0ab 8.3b 26.5b 11.4ab 19.7a 5.0 FLA 8580 48.1b 2.6b 7.4b 16.7b 7.9b 9.5b 4.0 HA 3074 (Inbar) 41.8b 2.7b 8.0b 14.2b 7.4b 6.1b 3.4 HA 3075 (Ofri) 45.4b 5.0b 4.4b 26.3ab 2.2b 6.1b 1.4 HA 3078 52.9b 11.6a 4.3b 14.5b 4.4b 14.2a 3.9 Tygress 75.4ab 2.9b 9.2ab 33.4ab 21.3a 6.0b 2.6 FL 47 (control) 69.8ab 3.2b 8.0b 15.6b 35.4a 4.6b 3.0 Sig. ** ** ** ** ** ** ns Roma Tomatoes Shanty 136.7 2.4 6.7 43.8 1.7 75.7 6.4 HA 3811 50.5 0.0 24.5 6.8 7.7 10.9 1.6 Sig. ** ns ** ** ** ** ns Blossom end scar (BES) Zipper and catface (Zip+CatF) Sunscald and yellow shoulder (SS + YS) Radial and concentric cracking (Crk) Odd shape (OS) Other cull categories (Other)

Post-Harvest Evaluation

Post-harvest as Firmness and Color Spring 2007 Varieties Firmness (pressure) Color (rating 1-10) Round Tomatoes BHN 745 45.2c 6.0b FLA 8576 45.0c 7.0a FLA 8579 45.3c 5.5b FLA 8580 45.2c 6.0b HA 3074 (Inbar) 45.7bc 5.0c HA 3075 (Ofri) 47.0ab 5.0c HA 3078 45.2c 6.0b Tygress 47.7a 4.0d FL 47 (control) 44.2c 5.5b Sig. ** ** Romas Tomatoes Shanty 46.3 5.0 HA 3811 45.6 7.0 Sig. ns **

BHN 745 Fla 8576 Fla 8579 Round Tomatoes Spring 2007 Fla 8580 Inbar Ofri HA 3078 Tygress FL 47

Roma Tomatoes Spring 2007 Shanty HA 3011

Field days with 65 attendees Spring 2007

Conclusions Under high virus pressure (spring 2007): HA 3078, 3074 (Inbar) and 3075 (Ofri). Tygress had high yield as well, but high percent of culls fruit with sunscald and yellow shoulder. The best roma variety was Shanty, but with high percent of cull fruits. In general the most firm fruits were the lowest color rating. Tygress very firm, but had low color rating. The most firm and highest color rating among the roma varieties was Shanty.

Thanks.thanks Thanks Southwest Vegetable Growers Association Sakata, Hazera, Harris Moran, Seminis, and BHN. Ready Plants and Six L Farms. For providing monetary or in-king support to this project.