Dale Leavitt. Oyster Grwout 1. Shellfish Grow-out - Oyster. Steps for growing oysters (or other bivalves) Growing strategy. Normal Course Sequence

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Shellfish Grow-out - Oyster Dale Leavitt Steps for growing oysters (or other bivalves) Normal Course Sequence Hatchery Growout Egg/sperm 25mm through through to market to 2mm Nursery 2mm through to 25 mm Growout Hatchery 25mm Egg/sperm through through to market to 2mm Growing strategy Oyster Grwout 1

Site Selection Let s start with the species American oyster From hatchery or spat collected in wild Will cover in more detail later Start with as big seed as you can afford! As you gain confidence, you can decrease the size of the seed you handle Size to move into growout system Depends on technology Can be anywhere between ¾ to 2 Don t invest more than you can afford to lose in the first year! Oyster Environmental Considerations Eastern Oyster (Crassostrea virginica ) Overall Range Optimal Range TEMPERATURE Culture Stage ( o C) ( o C) Spawning 20.0 Larval rearing 20.0-30.0 25.0 Juvenile to Adult -1.7-36.0 20.0-30.0 SALINITY Overall Range Optimal Range Culture Stage (ppt) (ppt) Larval rearing 3.1-30.6 24.5-29.8 Juvenile to Adult 0.0-42.0 14.0-28.0 DISSOLVED OXYGEN Critical Levels Culture Stage (mg/l) Larval growth <1 (for 11 h); optimal > 4.0 Juvenile to Adult <1 (for 5 days); optimal > 4.0 Overall Range Optimal Range ph Culture Stage Larval growth 6.0-9.0 6.75-8.75 TURBIDITY Critical level Culture Stage (mg/l) Juvenile to adult <750 WATER FLOW Optimal flow Culture Stage (cm/s) Juvenile to Adult > 10 SUBSTRATE Hard surface, prefer shell material Oyster Grwout 2

Optimal conditions For Grow-out: What technology options do I have? Land-based Intertidal Floating Subtidal Suspended On-Bottom On Bottom (Intertidal) Oyster Grwout 3

Sowing oyster seed Most growers free-plant at 1½ to 2 (about 1 year old) Use alternative technology for smaller seed Plant at density of 300-600 bushels of seed per acre (Moore 1897) 100-200 oysters/m 2 Split plots up into small units Separate oysters by unit size Spread evenly Also can place on bottom in bags Often used for short-term holding of market-sized oysters Bottom bag culture with a twist Oyster Grwout 4

Bottom Culture considerations Off Bottom Oysters in trays directly on bottom Oyster Grwout 5

Wire Mesh Oyster Tray configurations Large bottom trays Aquatray Oyster Grwout 6

A local Aquatray system The oyster bag (ADPI or OBC Bag) Oyster Bag: mesh details Oyster Grwout 7

Oyster Bag: end closure details Oyster Bag details Bag Closures Oyster Grwout 8

Oyster stocking density in bags Site specific - Rule of Thumb Generally put 4 6 liters (1 1.5 gallons) of biomass in a bag (test it out!!) For example: Ninigret Pond Growth rates seemed to be declining over time Tested for Food availability Water flow Stocking density Results Plenty of food in the water Water flow was minimal Stocking density critical Jiffy Pop syndrome Oyster Grwout 9

Oyster stocking density in bags Site specific - Rule of Thumb Generally put 4 6 liters (1 1.5 gallons) of biomass in a bag (test it out!!) If small oysters ½ (12.7mm) = 10,000 16,000 oysters/bag If 1 inch (25mm) = 1,700-2,700/bag If 2 inch (50mm) = 300-450/bag If markets (75mm) = 100-250/bag Jiffy Pop (Be Aware!!!!!) If start with 50,000 oysters Need 3-4 bags at 10mm Need 18 30 bags at 25mm Need 110 165 bags at 50 mm Need 200 500 bags at market size Plan Ahead!!! Rack & Bag The Rack Oyster Grwout 10

Rack & Bag A collapsible rack & bag system Australian Long-line System single backbone Oyster Grwout 11

Longline Oyster Bag System Double backbone system Seapa Bags Oyster Grwout 12

http://www.seapa.com.au/ BST Oyster Basket Comparison of technology (SEMAC Study) Overall Performance Rating Based on Total Unit Cost, Growth Performance, Durability, and Ease of Use Unit Type Cost Growth Performance Durability Ease of Use Rating Seapa Basket 1 1 1 1 4 Stars Aqua Tray 1.75 1.75 3.5 Stars Bag & Rack.5.5.5.5 2.0 Stars Oyster Grwout 13

Optimizing space use Another idea for growing oysters Flip bag oysters, is it worth the effort? Roger Williams University Oyster Grwout 14

RI Shellfish Aquaculture 8.27 million oysters sold ($5.6 million) 241 acres, 61 farms 81% increase in production in the past 10 years RI CRMC 2015 Product Quality and distinction is Increasingly Important! Product Quality Shell shape - Cup ratio (length:depth) - Fan ratio (lenth:width) Product Quality Shell shape - Cup ratio (length:depth) - Fan ratio (lenth:width) Shell condition - Hardness - Parasites (mud blisters, sponges) Oyster Grwout 15

Product Quality Meat condition plumpness & shell fill Product Quality Shell shape - Cup ratio (length:depth) - Fan ratio (lenth:width) Shell condition - Hardness - Parasites (mud blisters, sponges) Meat condition - plumpness - Shell fill CONSISTANCY Product Quality Growing superior grade oysters Tumbling Shaking bags Cleaning Air dry Floating gear Oyster Grwout 16

MHW 3' 1.7' 1' ADPI bag Poly float 30' 2' Seapa basket Poly float 6' 2.5' 2.5' Helical anchors Dale Leavitt Product Quality Growing superior grade oysters Flip Bags Tide tumbled 30' 1' 3' 2' 1.7' 6' MLW Poly float ADPI bag Seapa basket Poly float 2.5' 2.5' Helical anchors Hama Hama, WA Taylor Shellfish, WA Oysters LLC, AK? East Coast effort: NARF-Net Northeast Aquaculture Research Farm Network Established up to 5 demonstration/evaluation projects on farms in each of 5 northeast states (ME, NH, MA, RI, CT, & NY) Compare oyster morphometrics and CI between flip bags and traditional growing methods in place on each farm Started in summer 2014 and continued through summer 2016 Each state will host a farm demonstration day this winter (Funded by the NOAA National Sea Grant Extension Program) Darling Center, ME Dover, NH Point Judith, RI Oyster Grwout 17

RWU: Experimental set-up Do flip bags produce a superior grade oyster? Compare flip bag performance to conventional rack and bag and floating bag grow out methods - Adjacent systems - Same cohort Treatments: 5 replicates per treatment (Avg. starting size + SE) - ADPI flip (62.3 mm + 0.5) - Seapa flip (62.4 mm + 0.5) - Rack & bag (62.6 mm + 0.6) - Floating bag (avg. = 59.2 mm + 0.5) Stocking density - Rack & bag (150 oysters per bag) - Floating (150 oysters per bag) - Seapa (75 oysters per basket) RWU Experimental set-up Measured for morphometrics (length, width and depth) at intervals thru summer and fall Dept h Length Width Measured condition indices at end CI = [dry soft tissue weight (g) X 1000] / [total weight (g) shell weight (g)] RWU Rack & bag system Oyster Grwout 18

RWU Floating bag system RWU Flip bag system RWU Flip bag system Oyster Grwout 19

Growth (increase in valve length) Started on 23 June 2016 Ended on 3 November 2014 Growth (increase in valve length) Based on growth data, flip bags are less productive than rack & bag and floating gear. ADPI flip bag (avg. finish size = 62.6 mm + 0.7) ADG = 0.003 mm/day Seapa flip basket (avg. = 66.1 mm + 0.7) ADG = 0.03 mm/day Rack & bag (avg. = 77.9 mm + 0.9) ADG = 0.12 mm/day Floating bag(avg. = 83.5 mm + 1.6) ADG = 0.19 mm/day Too much tumbling to permit good shell growth? But does that tell all of the story? Length doesn t determine quality alone Oyster Grwout 20

Cup ratio (length:depth) AB A B C ADPI flip oysters have significantly deeper cups compared to rack & bag and floating bag oysters ADPI flip, Seapa flip and floating bag oysters have significantly deeper cups compared to rack & bag Fan ratio (length:width) AB AB B A Seapa flip oysters have significantly larger fan ratio compared to rack and bag oysters (p=0.002, α=0.05) Shell weight by length Higher shell weight per length in flip bag oysters 3.0 flip oysters (dry shell wt. = 41.35 g) 3.0 rack & bag oysters (dry shell wt. = 34.95 g) Oyster Grwout 21

Condition Index Flip bag oysters have significantly higher meat condition (p<0.0001, α=0.05) Meat weight by length Higher meat weight per length in flip bag oysters 2.5 flip oysters (dry meat wt. = 2.01 g) 3.0 rack & bag oysters (dry meat wt. = 1.69 g) Shell Fill Rack & Bag ADPI Flip Oyster Grwout 22

Blister Worms Rack & Bag 100% prevalence Grown adjacent to one another Both air dried at low tide Does physical disturbance drive the difference? ADPI flip 30% prevalence Does the flip bag increase oyster quality? An emphatic yes! Heavier shell More consistent grade More meat per unit of size Increased shell fill (observational) Reduced presence of mud blisters However. It slows down overall growth rate To compensate marketing Noticeably different product shorter but more meat heavier, cleaner shell Command a premium price to counter loss of growth (i.e. pay for extra time to achieve market size) Does the consumer care? Oyster Grwout 23

Does the consumer care? Market them at a Premium price West Coast kushi oysters (produced via flip bags) City Fish Company (Pike s Place, Seattle, WA) 1 doz farmed Pacifics - $13.99 1 doz kushi oysters - $19.99 Starvin Marvin s Seafoods (Blaine, WA) Pacifics (Fanny Bay 10 pack) - $6.96 kushi oysters (10 pack) - $11.10 The Lobsterman (Vancouver, BC) 1 doz Pacifics (Lighthouse Oyster) - $9.98 1 doz kushi oysters - $17.95 Average retail - about 60% price increase over a regular farmed oyster Other Considerations Total farm production? - Number of pieces per unit area Site selection - Shallow water (<2 MLW) - Minimal rocks (need to drive posts) Gear selection - Seapa/BST baskets are superior for handling and durability - Tension in line is critical Permitting - Highly visible at low tide - Navigational hazard if not properly marked - Currently permitted as floating gear in RI Acknowledgements: A group of RWU undergraduates were instrumental in assisting with this project: Heather Kinney Pandora Wadsworth Katchen Lachmyer Dan Arnold Tom Brubaker April Thibodeau Sean Maiorano Brie Mellen Tom Reynolds Sophia Gumbardo Sam Nunes Toby Adams-Cook So if you run into any of them hire them (but not Matt)!!!!! Oyster Grwout 24

Bottom cages Bottom Cage Bottom cages designs Oyster Grwout 25

Bottom Cage configurations Bottom cages in the intertidal Oyster Grwout 26

Handling Work Platform Oyster Grwout 27

Oyster cage hoist Considerations: Off-bottom Culture Some risks Oyster Grwout 28

Risks - Overwintering One solution: Overwintering oysters Suspended oyster culture Oyster Grwout 29

Pearl nets Oyster Grwout 30

Oysters in Lantern Net Hanging trays Surface Longline Oyster Grwout 31

RI Oyster Farm ~ Suspended Culture Oyster Grwout 32

Raft-based suspended culture Raft-based suspended culture Oyster Grwout 33

Suspended from a raft Stackable trays Nestier Tray Oyster Grwout 34

Aquatray Italian tray system Floating Oyster Grwout 35

Taylor Floats Oyster Grwout 36

Floating cage Oyster Grwout 37

Floating bag construction Floating bag floats Floating bag arrays Oyster Grwout 38

Floating/Submersible Oyster Bag System Business Plan (100 Bag Grid) Equipment Cost US $1,200 per grid Output Production @ 200 pcs. per bag 20,000 oysters per growout cycle $0.25 = $5,000 Market Value $0.35 = $7,000 $0.40 = $8,000 Equipment life span 8-12 years (amortized cost = $150 per year) Low labour cost; nature does bio-fouling control, shell pruning, shell hardening; lower production time One hectare at 50% surface coverage (allowing for navigation and carrying capacity recognition) will accommodate 45 grids Total output per hectare 675,000 to 900,000 oysters Gross income per hectare @ $0.25 = $195,000 average $168,750 - $225,000 Initial capital cost $54,000, amortized over 8 years; $6,750 per year x 2 year growout cycle $13,500 $182,500 Labor, fixed equipment usage, marketing, miscellaneous costs $70,000 Approximate Net Profit per Hectare $112,500 ($45,000/acre) Oyster Grwout 39

Floating Bag system economics Oyster Grow-out Methods cost comparison Oyster Grow-Out Methods - Capital Cost Per Unit Comparison Up-Front Deployment Up-Front Loading Amortized Life Span Comments Capital Cost Method Capital Cost Capacity Cost Per Unit Per Unit ALS 4000/13.46 44,550 0.0897 8-10 years 0.0099 Least labor Floating Bag 1400/14.00 15,000 0.0933 8-10 years 0.0103 2nd least labor System Aquatech Trays (deployed in 20.70 120 0.1725 10 years 0.0172 3rd least labor stacks of 7) Dark Sea Trays (deployed in 21.69 80 0.2711 10 years 0.0271 4th least labor stacks of 10) Mexican Trays (deployed in 12.69 80 0.1586 10 years 0.0158 4th least labor stacks of 10) French Rack & Bags - 6-8 years Most labor 16.35 200 0.09675 0.0138 Bag Rack - 10 years intensive Notes: Numbers for Aquatech Trays, Dark Sea Trays, and Mexican Trays are based on raft culture; cost per raft is $3,000 with 64 stacks per raft. These trays can also be deployed by long line which costs less ($1,800) but is more labor-intensive. French Rack & Bag System - Cost of one rack is $120 with a capacity of 8 bags. OysterGro Oyster Rancher Systems Oyster Grwout 40

Chessawanock Oyster Farm A few other odd ways to grow oysters! Rope oyster culture Oyster Grwout 41

Maintenance of oyster growout systems Management ~ fouling ~ Fouling Control Will discuss later with other pests Oyster Grwout 42

Density management Proper density Sorting Need to hold similar sized oysters together Bigger ones will outcompete smaller ones for food Should sort them on a regular basis A couple of times per summer As you are thinning densities Oyster Grwout 43

Tumbler/Sorter 4-barrel tumbler/sorter Oyster tumbler/sorter Oyster Grwout 44

Concentric tube tumbler/sorter Roll Your Own Oyster sorter/tumblers Dale Leavitt Roger Williams University I wanted to build a sorter/tumbler! Couldn t afford a production model Couldn t find much in terms of specifications for their operation Did a quick Google survey of materials related to sorter/tumblers Summarized what I found out that I could apply to my own design Oyster Grwout 45

Summary of tumbler designs ~Construction Materials~ QuickTube Sorter (http://chesbayoysterco.blogspot.com/2009/03/quicktubesorter.html) Aluminum frame with aluminum cylinder David Steele (https://www.youtube.com/watch?v=tzl9tnuifqm) PVC (Furniture grade) frame with PVC Cylinder Chris Philips (https://www.youtube.com/watch?v=pyzvhizuxdw) Aluminum frame with PVC cylinder Alaskan sorter (http://seagrant.uaf.edu/map/aquaculture/shellfish/techtraining/2010/tumblingandsorting.pdf) Wooden frame with wire mesh sorter My design Steel frame with concentric wire cylinders Summary of tumbler designs ~Mesh Sizes~ Quicktube Sorter Round holes ¼ to ½ for small seed 5/8 & 7/8 for seed tube 1¼ & 1¾ for submarket/market David Steele homemade tumbler Round holes 3/8 to 5/8 for very small seed 5/8 to 7/8 for small seed 3/4 to 7/8 for large seed 1 ¼ to 1 ¾ for markets Alaskan tumbler Square wire mesh ½ 1 1 ¾ Becy Oyster Mesh Company (http://pakbiz.com/profile/becy-oyster-mesh- Company/Oyster-sortingtumbler_PID47579.html) 0.5, 1.0, & 2.0 cm seed tube My final design Square wire mesh ¾, 1, & 1 ½ Summary of tumbler designs ~Tube lengths per grade~ QuickTube ~4 long with 3 grades (8 total) Chris Philips ~3 long with 3 grades (6 total) David Steele ~5 long with 3 grades (10 total) Alaskan ~2 long with 4 grades (6 total) My design ~4 long with 4 grades (4 total) Oyster Grwout 46

Summary of tumbler designs ~Rotation speed~ QuickTube 19 rpm David Steele 23.4 rpm Chris Philips 6.8 rpm Jack Blake 18 rpm My design 20 rpm Summary of tumbler designs ~Motor~ Needed to have a relatively low speed motor with good torque Initially decided on a treadmill motor Work at varying rpms People are giving them away! Unfortunately the one I acquired didn t work well Kept tripping the breaker Ended up using a ½ hp - 1750 rpm - 110VAC motor Have to gear it way down with pulleys to get to 20 rpm 2-stage pulley: 1.5 to 12 linked with a 1.5 to 15 Summary of tumbler designs ~Pitch~ Influences the rate that oysters move through the tube Made an estimate based on photos of tumbler/sorters that I could find pictures for The overall pitch seemed to be approximately 1 foot in 8 feet I built mine with no pitch and then adjust it by elevated the hopper end Have run it at 1:8 with good results Oyster Grwout 47

My final design Mesh size Spacing Diameter Circumference (inches) (inches) (inches) 1.5" 15 47 1" 2.5 20 63 0.75" 2.0 24 75 A Day in the Life of an Extension Agent ~ A Tumbler Story ~ A phone call Experiencing 50-75% mortality Near market size oysters Site visit Buckets of dead shell Opened up a few oysters Harvest Oyster Grwout 48

Dredging bottom planted oysters Oyster Grwout 49

Raking oysters Harvest from bags/trays easy! Grading & Culling Oyster Grwout 50

Electronic grader Machine grading Grading Oyster Grwout 51

Chessawanock Oyster Farm video https://www.youtube.com/watch?v=roolxzg 5s Oyster Grwout 52