Material Safety Data Sheet

ES800 Gram Stain Kit Page 1 of 8 M S D S Material Safety Data Sheet 24 HOUR EMERGENCY TELEPHONE NUMBER CALL CHEMTREC 1-800-424-9300 189 Twin County Road Morgantown, PA 19543 (610) 524-5810 ES800 is a gram stain kit, comprised of multiple components. The components are listed separately, according to product name. Product Identification Product Name: Product Code: Product Description: Gram's decolorizer GRAM'S DECOLORIZER ES800 (component of kit), ES801 (component of kit), ES805, ES811 A solution of acetone in reagent alcohol. Health 1 Flammability 3 Reactivity 0 Physical Hazard None Section 1 - Shipping Data DOT Shipping Name:* Ethyl Alcohol Tel. # for information: (610) 524-5810 DOT Hazard Class: Flammable Liquid Emergency Tel. #: (800) 424-9300 DOT Identification: UN1170 Prepared by: P.B. Packing Group: II Section 2 - Hazardous Ingredients / Identity Information CHEMICAL COMPONENTS CAS# % OSHA PEL ACGIH TLV ethanol 64-17-5 64% v/v 1000 ppm 1000 ppm isopropanol 67-63-0 3.6% v/v 400 ppm 400 ppm methyl alcohol 67-56-1 3.6% v/v 200 ppm (skin) 200 ppm (skin) acetone 67-64-1 25% v/v 1000 ppm 750 ppm TWA Section 3 - Physical / Chemical Characteristics

ES800 Gram Stain Kit Page 2 of 8 Boiling Point: 62-63 C Specific Gravity (H 20 = 1): 0.8 Vapor Pressure (mm Hg and Temperature): 213 @ 25 C Evaporation Rate ( n-butyl alcohol= 1): 1 Vapor Density (AIR=1): 2 Solubility in Water: Complete Appearance and Odor: A clear colorless solution with the characteristic odors of alcohol and acetone. Section 4 - Fire and Explosion Hazard Data Flash Point (Method Used): 24 C (TCC) Flammability Limits: (for ethanol) LEL 3% UEL 19% Extinguishing Media: Water spray, dry chemical, CO 2, foam for alcohol Special Fire Fighting Procedures: Wear protective clothing with NIOSH approved breathing apparatus. Products of combustion may be harmful in a fire situation. Do not use direct water stream. Unusual Fire and Explosive Hazards: Eliminate sources of ignition. Burns with invisible flame. Section 5 - Reactivity Data Stability: Stable Conditions to Avoid: Heat, sparks and flames Hazardous decompotion or byproducts: Oxides of carbon Hazardous polymerization: Does not occur Incompatibility (Materials to Avoid): Strong oxidizers. Precautions to be taken in Handling and Storage: Store at room temperature. Section 6 - Health Hazard Data Routes of Entry Inhalation? Skin Absorption? Ingestion? yes yes yes Carcinogenicity? NTP? IARC Monographs? OSHA Regulated? no no no yes Health Hazards (Acute and Chronic): Acute: Vapors may cause eyes, skin and respiratory irritation, drowsiness, nausea, headache and vomiting. Chronic: May cause liver, heart and kidney damage. May cause gastrointestinal disturbances and convulsions. Signs and Symptoms of Exposure: Vision blurring, drowsiness and headache. Medical Conditions Generally Aggravated by Exposure: Impaired kidney and liver function may be aggravated by exposure to alcohols. Preexisting eye, skin, and respiratory conditions may also be aggravated. Methanol has shown genetic toxicity in some animals. Emergency and First Aid Procedures: Seek medical assistance for further treatment, observation and support if necessary. Eye Contact: Thoroughly flush eyes with water. Contact a physician if irritation persists. Skin contact: Remove contaminated clothing and flush with water. Get medical attention if irritation persists. Ingestion: Rinse mouth thoroughly with water, provided person is conscious, and contact a physician. Section 7 - Precautions For Safe Handling and Use Steps to be Taken In Case of Spill Or Release: Eliminate source of ignition in vicinity of the spill or released vapor. Isolate the spill with sand or other absorbent material. Place all contaminated material in closed container for disposal. Waste Disposal Methods: All liquid and or contaminated material should be disposed of in DOT approved waste containers. Incineration for liquids is the suggested method of disposal. Comply with all Federal, State, and Local regulations for disposal. Precautions for handling and storage: Use precautions for flammable liquids. Avoid excessive heat and sources of ignition. For large quantities, store in flammable safety cabinet. Use in well-ventilated area. Section 8- Control Measures Respiratory Protection (Specify Type): Not normally required. If used in a poorly ventilated area use under an approved fume hood or NIOSH approved respirator. Ventilation: Special flammable exhaust hood if vapor concentration is high. In well-ventilated areas, mechanical exhaust is sufficient. Protective Gloves: Chemical resistant Eye Protection: Laboratory safety goggles or similar products are recommended. Other Protective Clothing And Equipment: Normal laboratory apparel suggested by Good Laboratory Practices Hygienic Work Practices: Wash thoroughly with soap and water after handling.

ES800 Gram Stain Kit Page 3 of 8 Product Identification Product Name: Safranin O, 0.4% Product Code: Product Description: SAFRANIN O, 0.4% MATERIAL SAFETY DATA SHEET ES800 (component of kit), ES801 (component of kit), ES806, ES812, ES812-16, ES812-2.5G A solution of Safranin O and water in reagent alcohol. Health 1 Flammability 3 Reactivity 0 Physical Hazard None Section 1 - Shipping Data Alcoholic solutions containing DOT Shipping Name:* less than 25% alcohol are not Tel. # for information: (610) 524-5810 regulated DOT Hazard Class: Not Applicable Emergency Tel. #: (800) 424-9300 DOT Identification: Not Applicable Prepared by: P.B. Section 2 - Hazardous Ingredients / Identity Information CHEMICAL COMPONENTS CAS# % OSHA PEL ACGIH TLV Ethanol 64-17-5 <19% 1000 1000/NR Methanol 67-56-1 <1% 200 200/250 Safranin O 477-73-6 <1% ------ ------ Section 3 - Physical / Chemical Characteristics Boiling Point: 198 F Specific Gravity (H 20 = 1): <1 Vapor Pressure (mm Hg and Temperature): 40 @ 20 C Evaporation Rate (ether= 1): >1 Vapor Density (AIR=1): 1.6 Solubility in Water: Complete Percent volatile by Vol.: 91% Appearance and Odor: A red/purple solution with the characteristic odors of alcohol. Section 4 - Fire and Explosion Hazard Data Flash Point (Method Used): 55 F (TCC) Flammability Limits: (for ethanol) LEL 3.5 UEL 19 Extinguishing Media: Water spray, dry chemical, CO 2, and foam for alcohol. Special Fire Fighting Procedures: Wear protective clothing with NIOSH approved breathing apparatus. Products of combustion may be harmful in a fire situation. Do not use direct water stream. Unusual Fire and Explosive Hazards: Eliminate sources of ignition. Burns with invisible flame. Section 5 - Reactivity Data

ES800 Gram Stain Kit Page 4 of 8 Stability: Stable Conditions to Avoid: Excessive heat, sparks or flames Incompatibility (Materials to Avoid): Strong oxidizers and organic acids. Hazardous decomposition or byproducts: Oxides of carbon and nitrogen. Hazardous polymerization: Does not occur. Precautions to be taken in Handling and Storage: Store at room temperature. Section 6 - Health Hazard Data Routes of Entry Inhalation? Skin Absorption? Ingestion? yes yes yes Carcinogenicity? NTP? IARC Monographs? OSHA Regulated? no no no yes Health Hazards (Acute and Chronic): Acute: Vapors may cause eyes, skin and respiratory irritation, drowsiness, nausea, headache and vomiting. Chronic: May cause liver, heart and kidney damage. May cause gastrointestinal disturbances and convulsions. Signs and Symptoms of Exposure: Vision blurring, drowsiness, and headache. Medical Conditions Generally Aggravated by Exposure: Impaired kidney and liver function may be aggravated by exposure to alcohols. Preexisting eye, skin, and respiratory conditions may also be aggravated. Methanol has shown genetic toxicity in some animals. Emergency and First Aid Procedures: Seek medical assistance for further treatment, observation and support if necessary. Eye Contact: Thoroughly flush eyes with water. Contact a physician if irritation persists. Skin contact: Remove contaminated clothing and flush with water. Get medical attention if irritation persists. Ingestion: Rinse mouth thoroughly with water, provided person is conscious and contact a physician. Inhalation: Remove person to fresh air. If not breathing, give artificial respiration and contact physician. Section 7 - Precautions For Safe Handling and Use Steps to be Taken In Case of Spill Or Release: Eliminate source of ignition in the vicinity of the spill or released vapor. Isolate the spill area with sand, or other absorbent material. Place all contaminated material in closed container for disposal. Waste Disposal Methods: All liquid and/or contaminated material should be disposed of in DOT approved waste containers. Incineration for liquids is the suggested method of disposal. Comply with all Federal, State and Local regulations for disposal. Precautions for handling and storage: Use precautions for flammable liquids. Avoid excessive heat and sources of ignition. For large quantities, store in flammable safety cabinet. Use in well ventilated area. Section 8- Control Measures Respiratory Protection (Specify Type): Not normally required. If used in a poorly ventilated area use under an approved fume hood or NIOSH approved respirator. Ventilation: Special flammable exhausted hood if vapor concentration is high. In well-ventilated areas, mechanical exhaust is sufficient. Protective Gloves: Chemical resistant Eye Protection: Laboratory safety goggles or similar products are recommended. Other Protective Clothing And Equipment: Normal laboratory apparel suggested by Good Laboratory Practices. Hygienic Work Practices: Wash thoroughly with soap and water after handling.

ES800 Gram Stain Kit Page 5 of 8 Crystal Violet MATERIAL SAFETY DATA SHEET Product Identification Product Name: Crystal Violet, 1% Product Code: ES800 (component of kit), ES801(component of kit), ES802, ES808 Product Description: An aqueous solution of crystal violet. Health 1 Flammability 1 Reactivity 0 Physical Hazard None Section 1 - Shipping Data DOT Shipping Name:* Not applicable Tel. # for information: (610) 524-5810 DOT Hazard Class: Not applicable Emergency Tel. #: (800) 424-9300 DOT Identification: Unregulated Prepared by: P.B. Section 2 - Hazardous Ingredients / Identity Information CHEMICAL COMPONENTS CAS# % OSHA PEL, ACGIH TLV/STEL(PPM) Crystal Violet 548-62-9 <1.0% NR/NR Phenol 108-95-2 <1.0% 5/250 Methanol 67-56-1 <1.0% 200/250 Ethanol 64-17-5 ~11.0% 1000/NR Section 3 - Physical / Chemical Characteristics Boiling Point: ~200 F Specific Gravity (H 20 = 1): ~1 Vapor Pressure (mm Hg and Temperature): 79 @ 20 C Evaporation Rate (ether= 1): >1 Vapor Density (AIR=1): >1 Solubility in Water: Complete Percent volatile by Vol. 10% Appearance and Odor: Purple liquid, phenolic odor. Section 4 - Fire and Explosion Hazard Data Flash Point : 62 F Flammability Limits: LEL, N/A UEL, N/A Extinguishing Media: Water spray, dry chemical, CO 2 and foam for alcohol. Special Fire Fighting Procedures: Wear protective clothing with NIOSH approved breathing apparatus. Products of combustion may be harmful in a fire situation. Do not use direct water stream. Unusual Fire and Explosive Hazards: Eliminate potential sources of ignition. Section 5 - Reactivity Data

ES800 Gram Stain Kit Page 6 of 8 Stability: Stable Conditions to Avoid: Excessive heat. Incompatibility (Materials to Avoid): Strong oxidizers, chromic anhydride, perchloric acids, etc. Hazardous polymerization: Does not occur Hazardous decomposition or byproducts: Oxides of carbon Precautions to be taken in Handling and Storage: Avoid excessive heat and sources of ignition. Section 6 - Health Hazard Data Routes of Entry: Inhalation, skin, and ingestion Carcinogenicity: Not classified as a human carcinogen. Health Hazards Acute: Vapors may cause eyes, skin, and respiratory irritation, drowsiness, nausea, headache and vomiting. Chronic: May cause liver, heart and kidney damage. May cause gastrointestinal disturbances and convulsions. Signs and Symptoms of Exposure: Vision blurring, drowsiness and headache. Medical Conditions Generally Aggravated by Exposure: The manufacturer is unaware of any. Emergency and First Aid Procedures: Seek medical assistance for further treatment, observation and support if necessary. Eye Contact: Thoroughly flush eyes with water. Contact a physician if irritation persists. Skin contact: Remove contaminated clothing. Wash affected area with soap and water. Ingestion: Rinse mouth thoroughly with water, provided person is conscious and contact a physician. Inhalation: Remove person to fresh air. If not breathing, give artificial respiration and contact physician. Section 7 - Precautions For Safe Handling and Use Steps to be Taken In Case of Spill Or Release: Eliminate source of ignition in the vicinity of the spill or released vapor. Isolate the spill area with sand or other absorbent material. Place all contaminated material in closed container for disposal. Waste Disposal Methods: All liquid and/or contaminated material should be disposed of in DOT approved waste containers. Incineration for liquids is the suggested method of disposal. Comply with all Federal, State, and Local regulations for disposal Precautions for handling and storing: Use precautions for combustible liquids. Avoid excessive heat and sources of ignition. Use in ventilated area. Section 8- Control Measures Respiratory Protection (Specify Type): Not normally required. If used in a poorly ventilated area use under an approved fume hood or NIOSH approved respirator. Ventilation: Mechanical exhaust is sufficient. Protective Gloves: Chemical Resistant. Eye Protection: Safety glasses. Other Protective Clothing And Equipment: Normal laboratory apparel suggested by Good Laboratory Practices. Hygienic Work Practices: Wash thoroughly with soap and water after handling.

ES800 Gram Stain Kit Page 7 of 8 Iodine, Stabilized MATERIAL SAFETY DATA SHEET Product Identification Product Name: Product Code: Product Description: Iodine, stabilized ES800 (component of kit), ES803, ES809 Stabilized iodine solution for Gram Stain kit. Health 1 Flammability 0 Reactivity 0 Physical Hazard None Section 1 - Shipping Data DOT Shipping Name:* Not applicable Tel. # for information: (610) 524-5810 DOT Hazard Class: Not applicable Emergency Tel. #: (800) 424-9300 DOT Identification: Unregulated Prepared by: P.B. Section 2 - Hazardous Ingredients / Identity Information CHEMICAL COMPONENTS CAS# % OSHA PEL, ACGIH TLV/STEL(PPM) PVP Iodine 25655-41-8 <13% NE Potassium Iodide 7681-11-0 <2% NE Section 3 - Physical / Chemical Characteristics Boiling Point: 212 F Specific Gravity (H 20 = 1): ~1 Vapor Pressure (mm Hg and Temperature): 40 @ 20 C Evaporation Rate ( n-butyl alcohol= 1): >1 Vapor Density (AIR=1): 1 Solubility in Water: Complete Percent volatile by Vol. 5% Appearance and Odor: Redish-brown liquid, iodine odor. Section 4 - Fire and Explosion Hazard Data Flash Point : N/A Flammability Limits: LEL N/A UEL N/A Extinguishing Media: Water spray, dry chemical, CO 2 and foam. Special Fire Fighting Procedures: Wear protective clothing with NIOSH approved breathing apparatus. Products of combustion may be harmful in a fire situation. Do not use direct water stream. Unusual Fire and Explosive Hazards: None known Section 5 - Reactivity Data

ES800 Gram Stain Kit Page 8 of 8 Stability: Stable Conditions to Avoid: Excessive heat. Hazardous polymerization: Does not occur Hazardous decomposition or byproducts: Oxides of carbon Incompatibility (Materials to Avoid): Strong oxidizers, chromic anhydride, perchloric acids. Precautions to be taken in Handling and Storage: Avoid excessive heat. Section 6 - Health Hazard Data Routes of Entry: Inhalation, skin, and ingestion Carcinogenicity: Not classified as a human carcinogen. Health Hazards Acute: Vapors may cause eyes, skin, and respiratory irritation. Chronic: Poisoning may cause gastrointestinal disturbances, convulsions, circulatory problems and damage kidneys. Signs and Symptoms of Exposure: Skin and respiratory irritation. Medical Conditions Generally Aggravated by Exposure: The manufacturer is unaware of any. Emergency and First Aid Procedures: Seek medical assistance for further treatment, observation and support if necessary. Eye Contact: Thoroughly flush eyes with water. Contact a physician if irritation persists. Skin contact: Remove contaminated clothing. Wash affected area with soap and water. Ingestion: Rinse mouth thoroughly with water, provided person is conscious and contact a physician. Inhalation: Remove person to fresh air. If not breathing, give artificial respiration and contact physician. Section 7 - Precautions For Safe Handling and Use Steps to be Taken In Case of Spill Or Release: Isolate the spill area with sand, or other absorbent material. Place all contaminated material in a closed container for disposal. Waste Disposal Methods: All liquid and or contaminated material should be disposed of in DOT approved waste containers. Incineration for liquids is the suggested method of disposal. Comply with all Federal, State, and Local regulations for disposal Precautions for handling and storing: Use normal precautions for liquids. Avoid excessive heat. Use in ventilated area. Section 8- Control Measures Respiratory Protection (Specify Type): Not normally required. If used in a poorly ventilated area use under an approved fume hood or NIOSH approved respirator. Ventilation: In ventilated areas, mechanical exhaust is sufficient. Protective Gloves: Chemical Resistant. Eye Protection: Safety glasses. Other Protective Clothing And Equipment: Normal laboratory apparel suggested by Good Laboratory Practices. Hygienic Work Practices: Wash thoroughly with soap and water after handling. AZER SCIENTIFIC INC. MAKES NO REPRESENTATION AS TO THE COMPREHENSIVENESS OR ACCURACY OF THIS INFORMATION. INDIVIDUALS RECEIVING THIS INFORMATION MUST EXERCISE THEIR INDEPENDENT JUDGMENT IN DETERMINING ITS APPROPRIATENESS FOR A PARTICULAR PURPOSE. ACCORDINGLY, AZER SCIENTIFIC INC. WILL NOT BE RESPONSIBLE FOR DAMAGES OF ANY KIND OR NATURE RESULTING FROM THE USE OF ANY SUCH INFORMATION. NO REPRESENTATIONS OR WARRANTIES OF ANY KIND OR NATURE, INCLUDING BUT NOT LIMITED TO, EXPRESS WARRANTIES, IMPLIED WARRANTIES OF MERCHANTABILITY, OR WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE, ARE MADE HEREUNDER WITH RESPECT TO THE INFORMATION SET FORTH HEREIN OR TO THE PRODUCT TO WHICH THE INFORMATION REFERS.