No persistent behavioural effects of SCUBA diving on reef sharks

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The following supplement accompanies the article No persistent behavioural effects of SU diving on reef sharks Darcy radley*, Yannis P. Papastamatiou, Jennifer E. aselle *orresponding author: darcybradley@ucsb.edu Marine Ecology Progress Series 67: 7 8 (7) Table S. Species list. ll predator species observed in baited remote underwater video (RUV) surveys at Palmyra toll. Family Genus Species Species ode arangidae arangoides orthogrammus OR arangidae aranx ignobilis IG arangidae aranx melampygus ME arangidae Elagatis bipinnulata ELI arangidae Scomberoides lysan SLY archarhinidae archarhinus amblyrhynchos archarhinidae archarhinus melanopterus ML archarhinidae Triaenodon obesus TRO hanidae hanos chanos HH Lethrinidae Gnathodentex aureolineatus GNU Lethrinidae Lethrinus olivaceus LEOL Lethrinidae Lethrinus xanthochilus LEX Lethrinidae Monotaxis grandoculis MOGR Lutjanidae phareus furca PFU Lutjanidae prion virescens PVI Lutjanidae Lutjanus bohar LUO Lutjanidae Lutjanus fulvus LUFU Lutjanidae Lutjanus gibbus LUGI Muraenidae Gymnothorax javanicus GYJ Serranidae ephalopholis argus ER Serranidae ephalopholis leopardus ELE Serranidae ephalopholis miniata EMI Serranidae ephalopholis urodeta EUR Serranidae Epinephelus howlandi EPHO Serranidae Epinephelus melanostigma EPML Serranidae Epinephelus polyphekadion EPPO Serranidae Epinephelus spilotoceps EL Serranidae Epinephelus tauvina EPT Serranidae Gracila albomarginata GRL Serranidae Variola louti VLO

6 PengSpit PengSpitNR NR NR PengSpit PengSpitNR NR NR PengSpit PengSpitNR NR NR 6 PengSpit PengSpitNR NR NR PengSpit PengSpitNR NR NR PengSpit PengSpitNR NR NR! Dived! Undived!!! MaxN!!! Tarrive (min) Time MaxN (min) NR NR!!!! NR NR NR NR NR NR 6!!! E 6!!!!!! D NR NR F NR NR Fig. S. Paired RUV survey results. MaxN, time of MaxN, and T arrive for and blacktip reef sharks at paired dived and undived sites at Palmyra toll.

Time MaxN (min) MaxN 6 8 8! 6!!! 6 8 8! 6!!! D Tarrive (min) 6! 6!!!!! E 6! 6!!!!! F Fig. S. aited remote underwater video system (RUVS) deployment methods. amera deployment method (lowered from boat vs. diver-deployed) did not affect MaxN, time of MaxN, and T arrive for sharks (,, E) or sharks (, D, F).

MaxN Penguin Spit 8 9 9 9 9 9 8/ 9/ 9/ 9/ 9/ 9/ Penguin Spit NR 8 9 98 9 9 9 8 9 9 98 9 9 9 9 9 96 99 9 9 8 9 9 96 9 9 8/ 9/ 9/8 9/ 9/ 9/ 8/ 9/ 9/ 9/8 9/ 9/ 9/ 9/ 9/ 9/6 9/9 9/ 9/ 8/ 9/ 9/ 9/6 9/ 9/ 9 9 98 9 9 9 9 9 96 98 9 9 8 9 9 96 98 99 9 9/ 9/ 9/8 9/ 9/ 9/ 9/ 9/ 9/6 9/8 9/ 9/ 8/ 9/ 9/ 9/6 9/8 9/9 9/ NR NR Time MaxN (min) 8 6 8 6 8 6 8 6 Penguin Spit 8 9 9 9 9 9 8 9 98 9 9 9 8 6 8 6 Penguin Spit NR 9 9 98 9 9 9 8 9 9 98 9 9 9 8 6 8 6 9 9 96 98 9 9 9 9 96 99 9 9 8 6 8 6 8 9 9 96 98 99 9 8/ 9/ 9/ 9/ 9/ 9/ 9/ 9/ 9/8 9/ 9/ 9/ 9/ 9/ 9/6 9/8 9/ 9/ 8/ 9/ 9/ 9/6 9/8 9/9 9/ NR NR 8 9 9 96 9 9 8/ 9/ 9/8 9/ 9/ 9/ 8/ 9/ 9/ 9/8 9/ 9/ 9/ 9/ 9/ 9/6 9/9 9/ 9/ 8/ 9/ 9/ 9/6 9/ 9/ 6 6 Penguin Spit 6 Penguin Spit NR 6 6 Tarrive (min) 6 6 8 8/ 9 9/ 9 9/ 9 9/ 9 9/ 98 9/8 9 9/ 9 9 96 9/ 9/ 9/6 98 9 9 9/8 9/ 9/ 8 8/ 96 9/6 98 9/8 6 NR 6 6 NR 8 9 9 9 8 9 9 Date 9 9 96 99 9 8 9 9 8/ 9/ 9/ 9/ 8/ 9/ 9/ 9/ 9/ 9/6 9/9 9/ 8/ 9/ 9/ Fig. S. aited remote underwater video system (RUVS) repeat sampling. MaxN, time of MaxN, and T arrive for sharks are shown for each day at each surveyed site.

shark! shark Time MaxN (min) MaxN 8! 6!!! (,] (,] (,] (,] 6!! (,] (,] (,] (,] Tarrive (min)!!!! (,] (,] (,] (,] - - - - Depth (m) Fig. S. aited remote underwater video system (RUVS) depths. Survey depth did not affect MaxN, time of MaxN, or T arrive for or sharks.

,, Predator species richness! PengSpit PengSpitNR NR NR PengSpit PengSpitNR NR NR Fig. S. Predator species richness. Only one site,, had significantly lower species richness than all other sites (one-way NOV, F(7, 6) =., p <.). Different letters (,, ) indicate a significant difference (Tukey HSD, p <.). 6

Fig. S6. Grey reef shark underwater visual surveys. Grey reef sharks per belt transect survey (left axis) and towed diver survey (right axis) on Palmyra s forereefs between -8. Error bars represent +/- SE. Data provided by NO REP. 7