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University of Otgo Lbortory helth nd sfety udit tool Deprtment: Loction Detils: DLM: Building: Room: Dte: Nme Of Auditor(s) Signture of Auditor(s) Lbortory Designtions: HSNO Exempt Rdionuclide Lbortory X-Ry Lbortory Lser Lbortory PC2 Clinicl Other: Is the lbortory prt of MPI registered continment/trnsitionl fcility? NO YES If yes plese complete detils below: PC Level: PC1 PC2 PC3 PC Type: Microbiologicl Animl Plnt Invertebrte Other: Corrective ctions identified Completed during udit? No Yes Dte Entered in Vult 1 P g e

A Lbortory Fcilities (Stt Budget Audit Items) Yes No n Comment/Notes 1 Lbortory cn be secured when not occupied. 2 Door signge correct (includes correct lb designtions, uthorised personnel only nd pproprite pictogrms) 3 Bench-top surfces impervious to liquids, resistnt to chemicls in use nd ble to withstnd het. 4 Floors non-slip, esy to clen, impervious to liquids nd resistnt to chemicls in use. 5 Wlls smooth, impervious to liquids, esy to clen, impermeble to liquids nd resistnt to chemicls in use. 6 Ceilings constructed of rigid, smooth-fced, wshble mteril. 7 Lbortory furniture ergonomiclly suitble nd coverings impervious to liquids. 8 All fixtures, fittings nd equipment (including under-bench cupbords) designed to llow redy ccess to floor for clening. 9 Internl fittings (e.g. lights, ducts) designed to minimise horizontl dust-collecting services. 10 Shelving provided with erth quke restrints nd is fixed to wll or other fixed support. 11 Gps round pipework nd other penetrtions hve been seled. 12 Skirting bords in good condition or flooring coved to wll. 13 Noticebords impervious to liquids (cn be wiped clen) 14 Vrnished surfces (e.g. window sills, benches, shelves) in good condition. 15 Approprite provision of chemicl storge cbinets for storge of: Flmmble liquids (Clss 3) b Flmmble solids (Clss 4) c Oxidizers (Clss 5) d Toxic (Clss 6) e Corrosives (Clss 8) 16 Gs cylinder restrints vilble. 17 Seprte write-up res provided outside of lbortory. 18 Sufficient hooks/storge for lbortory cots provided djcent to min lbortory exit. 19 Hnd-wsh fcilities provided djcent to exit from lbortory nd ble to foot, elbow or electroniclly operted. 20 Lbortory sinks with hot nd cold wter provided. 21 Emergency eye-wsh fcilities vilble (no more thn 15m from ny point in the lbortory) nd ble to be used s drench hose. 22 Sfety Shower provided. 23 Gs shut-off vlve vilble nd clerly identified. 24 Lbortory is mechniclly ventilted. 25 Negtive ir pressure reltive to djcent non-pc2 res n/ for non-pc2 lbs mintined (i.e. direction of ir movement is into PC2 fcility). 26 Recircultion into re outside PC2 fcility does not occur. n/ for non-pc2 lbs 2 P g e

B Opertionl Requirements Yes No n Comment 1 Are hzrdous substnces ppropritely segregted ccording to their mutully comptibility nd clss? 2 Are hzrdous substnce storge res clerly identified with the mjor hzrd present? 3 Are corrosives stored in mnner tht ensures segregtion of: Oxidizing cids from flmmble or combustible substnces (including orgnic cids such s glcil cetic cid) b Strong bses from strong cids c Other incomptible substnces (e.g. cynides, zides) 4 Are ll continers in good condition nd clen (i.e. free of residue/spills on outside). 5 Are the contents of unttended working continers clerly identified? 6 Is secondry continment provided for liquid substnces? 7 Storge of hzrdous substnces in refrigertors: Fridges used for storge of hzrdous substnces lbelled with pproprite hzrd symbols. Are incomptible substnces ppropritely segregted within b the fridge? Fridges used for storing flmmble liquids re designed or c modified to eliminte internl sources of ignition (e.g. lights, fns, thermostt switches). Fridges not designed/modified for storge of flmmble d liquids clerly signed s Not to be used for storge of flmmble liquids or similr. 8 All gs cylinders ppropritely restrined s below: Lrge cylinders double-chined (top nd bottom) using pproprite gs cylinder restrint b Smll cylinders single chined or in stnd. 9 No more thn two cylinders of ech gs formultion stored djcent to ech instrument. 10 Flsh-bck rrestors fitted between cylinders of flmmble gses nd equipment where gs used. 11 Benches clen nd free of clutter, chemicl residue nd shrps. 12 Floors clen, free of clutter nd trip/slid hzrds (pools of liquid, ice, boxes, cbles, dmged floor tiles etc.) 13 Items of portble electricl equipment hve been electriclly tested nd tgged within the lst 12 months. 14 Fume hood certifiction current. 15 Fume hoods clen, free of clutter nd not used for storge of chemicls. 16 Lbortory cots, Sfety Glsses nd disposble gloves vilble. 17 Personnel working in lbortory re wering pproprite PPCE (including pproprite footwer). 3 P g e

C Emergency Mngement nd Documenttion Yes No n Comment 1 University of Otgo Emergency Procedures vilble? 2 Are the following emergency contct detils provided djcent to lbortory phones? Emergency services? b d The building street ddress? Contct detils for key personnel (e.g. lb mngers, helth nd sfety personnel, security) 3 First Aid Kit vilble? 4 Fire extinguishers vilble nd pproprite for substnces in use? 5 Fire blnkets vilble. 6 Chemicl spill kit vilble. 7 Sfety Dt Sheets redily vilble? 8 Is n inventory mintined which documents the following for ll hzrdous substnces present 9 The identity of ny substnces present? b The quntity (continer size) of ny substnces present? c The hzrd clssifictions of ny substnces present? 10 Trcked substnces recorded, including records of ll disposls or trnsfers in lst 12 months? Notes/comments 4 P g e

NOTE: This section only needs to be completed by Sector Mngers for MPI-registered Physicl Continment Lbortories only D Sector Mnger Checklist (MPI Registered Fcilities Only) Yes No n Comment 1 Is ccess to the lbortory is limited to specified personnel? 2 Are trining registers vilble nd if so re they up to dte? 3 Does the lbortory hold registers for unclered biologicl mterils nd new orgnisms? 4 Current Continment nd Trnsitionl Fcility Mnul vilble? 5 A biologicl spill kit contining SOP for clening spills is vilble? 6 Stndrd Operting Procedures (SOPs) PC1 nd PC2 SOP Version 1 Current version present? b Hve ll old versions of the PC1 nd PC2 SOP been removed? 7 Disinfectnts: Avilble for decontmintion or snittion use in the lbortory (record expiry dte) b Present in biologicl spill kit (record expiry dte) Wht disinfectnt is used to wipe down work surfces wiped c down t the end of ech work period (list in comments section)? 8 Biologicl wste disposl: Describe the wste disposl pthwys used in the comments section. b All microbiologicl wste is utoclved before disposl? All contminted glsswre is utoclved or chemiclly c disinfected prior to wshing nd reuse? 9 Work prctices: Are food nd drink present in the lbortory? b Is Long hir tied bck? c Are incidents/ccidents recorded? d Hve there been ny significnt spills or ccidents this yer? e Are cultures clerly identified nd ppropritely stored? f Are wter bths mintined in clen stte? Are benches free of clutter nd other surfces resonbly g dust free? h Biohzrd wste bgs re NOT over full? 10 Storge of risk mteril: Are biohzrd symbols plced on ll cupbords, refrigertors nd freezers tht contin risk mteril? If other items re present, is the risk mteril in continer b with biohzrd symbol nd code tht reltes it bck to the BACC/MPI movement uthority tht ccompnied it? c Freezers in public spces re kept locked? 11 Additionl requirements for PC2: Are the lbortory doors normlly closed when work is in progress? b Continers for infectious mterils/wste c Is Clss II biologicl sfety cbinet (BSC) vilble? 5 P g e

d e f g h i Is the Clss II BSC inspected by qulified inspector t lest nnully (record retest dte)? Is the Clss II BSC used for procedures tht my generte erosols, such s shking, mixing nd ultrsonic disruption? All mteril of humn origin hndled in Clss II BSC? Is centrifuge fitted with seled rotors or cups used if lrge volumes or high concentrtions of infectious mteril used? Are wshble keybords or protective covers provided for keybords on workbenches? Are vible orgnisms trnsported double contined between lbortories? UOOLAB4-2015 Notes/comments 6 P g e

7 P g e