I 29/01/2015 School of Molecular Bioscience, University of Sydney Worker induction - Hazard assessment form - This form should be completed by the new student/staff member together with the supervisor. If new tasks of hazardous agents being introduced at a later time, please append this document or use a new form. - It is important that the new worker has an opportunity to ask questions about any hazards and risks. - A copy of this document (and any modifications, additions) must be sent to the School s Safety Officer. Name of worker (print) Name of supervisor (print) Work location(s) (building and room numbers) PART A: List hazardous agents / equipment, nature of the hazards, and risk control measures (tick boxes, provide (tick boxes, provide (tick boxes, provide * In addition to the controls listed here, all lab workers in all areas (teaching, research, prep/washup) must wear enclosed shoes, a clean, buttoned-up, properly-fitting lab coat, and any long hair tied back. ** Provide details in these cases.
II 29/01/2015 Part A: List hazardous agents / equipment, nature of the hazards, and risk control measures (tick boxes, provide (tick boxes, provide (tick boxes, provide (tick boxes, provide Please insert/print additional pages if more agents/tasks are used.
III 29/01/2015 PART B: Standard operating procedures, risk assessments, safety data sheets. The supervisor needs to confirm here which Standard Operating Procedures (SOPs) and Risk Assessments (RAs) are appropriate for this worker (tick the item as appropriate). These documents are available on the SMB website (http://sydney.edu.au/science/molecular_bioscience/ohs/). If an appropriate SOP/RA does not exist, this needs to be created before work begins. If new SOPs or RAs are added at a later time, this has to be dated and initialled by supervisor and worker. SMB SOP No. Procedure SOP required RA required Other 001 Agarose gel electrophoresis 002 Working with animals and animal tissues 003 Autoclaving 004 Cleanup of biohazard spills 005 Use of biosafety II cabinet 006 Using Bunsen burners 007 008 Centrifugation High speed floor centrifuges Disposal of hazardous chemical waste 009 Working with corrosives 010 011 012 013 Cryogenics including liquid nitrogen DNA or RNA extraction using spin columns DNA purification using phenol- chloroform Use, storage and disposal of flammable liquids 014 Freeze drying (Cryodos machine) 015 Freeze drying (Martin Christ machine) 016 Using a fume hood 017 Handling storage and use of gas cylinders 018 Scientific glassware washing
IV 29/01/2015 019 High pressure liquid chromatography (HPLC) 020 Use of microwave oven 021 022 Nuclear magnetic resonance (NMR) spectroscopy Northern blotting and P32 probe hybridisation 023 Protein Purification 024 025 026 027 028 Working with quarantine (DAFF- AQIS) materials Working with Risk Group 1 microorganisms Working with Risk Group 2 microorganisms Using small- angle scattering X- ray generator (SAXSess Lab) SDS- PAGE (polyacrylamide gel electrophoresis) 029 Eyewash and safety shower testing 030 Sonication 031 032 Southern blotting and P32 probe hybridisation Spread- plating with glass spreader and ethanol 033 Tissue culture work 034 Handling toxic chemicals 035 036 Working with Phosphorus- 32 and - 33 radioactive isotopes Disposal of GMO (Genetically Modified Organism)- contaminated waste 037 Using X- ray cryosystems 038 Using an X- ray power generator 039 Biohazard Waste 040 Using the CP1000 film processor
V 29/01/2015 041 Communal PC1 cell culture lab 042 Communal PC2 cell culture lab 043 044 045 Cleanup of mercury spill from broken thermometer Working with tritium 3H radioactive isotope Working with Iodine- 125 radioactive isotope 046 Use and disposal of sharps 047 048 049 Working with human blood and tissue General staff and external contractors in PC2 laboratories Working with Risk Group 2 microorganisms in teaching labs 050 Using a rotory evaporator 051 Operation and maintenance of the ToxiRAE Pro CO2 monitor 052 Contents of spill kits 053 Working with benzene 054 Working with tamoxifen 055 Transport, loading and filling of liquid nitrogen containers offsite
VI 29/01/2015 PART C: Safety Data Sheets List any safety data sheets (SDS, MSDS, PSDS) that are relevant (please attach list on separate page if needed). All relevant SDS must be read and understood by the worker prior to using the substances. PART C: Location / type of emergency equipment available: Safety shower & eyewash Spill kit. Fire fighting... Other.. PART D: Summary of risk to new worker: Tick the appropriate box (indicate the highest level of risk of all agents or tasks listed above). The risk is insignificant and is unlikely to increase during the course of the project. The risk is significant but will be effectively controlled. There is uncertainty about the level of risk. The risk is significant and cannot be effectively controlled. PLEASE NOTE: Work must not commence if either There is uncertainty about the level of risk or The risk is significant and cannot be effectively controlled has been selected. PART C: Declarations I have read and understood the above-described SOP, RA, and SDS documents Worker signature:... Date:. Supervisor and worker signatures Supervisor... Worker. Date. Date. By signing here, the supervisor and worker confirm that they have discussed the risks of the project, that the worker has been given a chance to ask questions relating to the safety of the work to be performed, that the worker has been provided with adequate resources and information to perform their work safely, and that both partners agree on the summary of risk (appropriate box checked above).