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Creation Date 20-Aug-2009 Material Safety Data Sheet 1. PRODUCT AND COMPANY IDENTIFICATION Revision Number 2 Product Name Tryptic Soy Broth (TSB) Cat. Synonyms Recommended Use Company HyClone Laboratories, Inc. 925 West 1800 South Logan, UT 84321United States Tel: (435) 792-8000 CH3A033, SH20622, SH30362, SH30363, SH30364, SH30365, SH30366, SH30367, SH30368, SH30369, SH30523, SH30567, SH30902, SH30905, SH3A739, SH3A793, SH3A1049, SH3A1148, SH3A1536, SH3A1628, SH3A1688, SH3A1701, SH3A1747, SH3A2001, SH3A2113, SH3A2199, SH3A2285, SH3A2292, SH3A2468, SH3A2479 In vitro methods Emergency Telephone Number INFOTRAC - 24 Hour Number: 1-800- 535-5053 Outside of the United States, call 24 Hour Number: 001-352-323-3500 (Call Collect) 2. HAZARDS IDENTIFICATION CAUTION! Emergency Overview May cause eye, skin, and respiratory tract irritation. Low hazard for usual industrial or commercial handling. The toxicological properties have not been fully investigated. Appearance Beige Physical State viscous liquid odor Characteristic Target Organs Potential Health Effects Acute Effects Principle Routes of Exposure Eyes Skin Inhalation ne known. May cause irritation May cause irritation May cause irritation of respiratory tract Page 1 / 7

Ingestion Chronic Effects Ingestion may cause gastrointestinal irritation, nausea, vomiting and diarrhea ne known. See Section 11 for additional Toxicological information. Aggravated Medical Conditions 3. COMPOSITION/INFORMATION ON INGREDIENTS Haz/n-haz Component CAS- Weight % Tryptic Soy Broth NA </= 100 4. FIRST AID MEASURES Eye Contact Skin Contact Inhalation Ingestion tes to Physician Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Obtain medical attention. Treat symptomatically. 5. FIRE-FIGHTING MEASURES Flash Point Method Autoignition Temperature Explosion Limits Upper Lower Suitable Extinguishing Media Unsuitable Extinguishing Media Hazardous Combustion Products Sensitivity to mechanical impact Sensitivity to static discharge data available data available Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide. Specific Hazards Arising from the Chemical ne known. Page 2 / 7

Protective Equipment and Precautions for Firefighters As in any fire, wear self-contained breathing apparatus pressure-demand, MSHA/NIOSH (approved or equivalent) and full protective gear NFPA Health 0 Flammability 0 Instability 0 Physical hazards N/A 6. ACCIDENTAL RELEASE MEASURES Personal Precautions Environmental Precautions Methods for Containment and Clean Up Use personal protective equipment. Ensure adequate ventilation. Avoid contact with skin, eyes and clothing. Should not be released into the environment. Soak up with inert absorbent material. Keep in suitable and closed containers for disposal. 7. HANDLING AND STORAGE Handling Storage Wear personal protective equipment. Avoid contact with skin, eyes and clothing. Do not breathe vapors or spray mist. Keep containers tightly closed in a dry, cool and well-ventilated place. 8. EXPOSURE CONTROLS / PERSONAL PROTECTION Engineering Measures Exposure Guidelines Ensure adequate ventilation, especially in confined areas. Ensure that eyewash stations and safety showers are close to the workstation location. This product does not contain any hazardous materials with occupational exposure limits established by the region specific regulatory bodies. NIOSH IDLH: Immediately Dangerous to Life or Health Personal Protective Equipment Eye/face Protection Skin and body protection Respiratory Protection Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166 Wear appropriate protective gloves and clothing to prevent skin exposure Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced 9. PHYSICAL AND CHEMICAL PROPERTIES Physical State Appearance odor Odor Threshold ph Vapor Pressure Vapor Density Viscosity Boiling Point/Range Melting Point/Range viscous liquid Beige Characteristic Page 3 / 7

Decomposition temperature Flash Point Evaporation Rate Specific Gravity Solubility log Pow 9. PHYSICAL AND CHEMICAL PROPERTIES data available 10. STABILITY AND REACTIVITY Stability Conditions to Avoid Incompatible Materials Hazardous Decomposition Products Hazardous Polymerization Hazardous Reactions. Stable under normal conditions. Incompatible products. Excess heat. Oxidizing agents ne known Hazardous polymerization does not occur. ne under normal processing. 11. TOXICOLOGICAL INFORMATION Acute Toxicity Product Information acute toxicity information is available for this product Component Information Irritation Toxicologically Synergistic Products Chronic Toxicity Carcinogenicity There are no known carcinogenic chemicals in this product Sensitization Mutagenic Effects Reproductive Effects Developmental Effects Teratogenicity Other Adverse Effects The toxicological properties have not been fully investigated.. Page 4 / 7

Endocrine Disruptor Information information available 12. ECOLOGICAL INFORMATION Ecotoxicity Do not empty into drains. Persistence and Degradability Bioaccumulation/ Accumulation Mobility information available information available information available 13. DISPOSAL CONSIDERATIONS Waste Disposal Methods Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. Chemical waste generators must also consult local, regional, and national hazardous waste regulations to ensure complete and accurate classification 14. TRANSPORT INFORMATION DOT t regulated TDG t regulated IATA t regulated IMDG/IMO t regulated 15. REGULATORY INFORMATION International Inventories Legend: X - Listed E - Indicates a substance that is the subject of a Section 5(e) Consent order under TSCA. F - Indicates a substance that is the subject of a Section 5(f) Rule under TSCA. N - Indicates a polymeric substance containing no free-radical initiator in its inventory name but is considered to cover the designated polymer made with any free-radical initiator regardless of the amount used. P - Indicates a commenced PMN substance R - Indicates a substance that is the subject of a Section 6 risk management rule under TSCA. S - Indicates a substance that is identified in a proposed or final Significant New Use Rule T - Indicates a substance that is the subject of a Section 4 test rule under TSCA. XU - Indicates a substance exempt from reporting under the Inventory Update Rule, i.e. Partial Updating of the TSCA Inventory Data Base Production and Site Reports (40 CFR 710(B). Page 5 / 7

15. REGULATORY INFORMATION Y1 - Indicates an exempt polymer that has a number-average molecular weight of 1,000 or greater. Y2 - Indicates an exempt polymer that is a polyester and is made only from reactants included in a specified list of low concern reactants that comprises one of the eligibility criteria for the exemption rule. U.S. Federal Regulations TSCA 12(b) SARA 313 SARA 311/312 Hazardous Categorization Acute Health Hazard Chronic Health Hazard Fire Hazard Sudden Release of Pressure Hazard Reactive Hazard Clean Water Act Clean Air Act OSHA CERCLA t Applicable California Proposition 65 This product does not contain any Proposition 65 chemicals. State Right-to-Know U.S. Department of Transportation Reportable Quantity (RQ): N DOT Marine Pollutant N DOT Severe Marine Pollutant N U.S. Department of Homeland Security This product does not contain any DHS chemicals. Other International Regulations Mexico - Grade information available Canada This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations (CPR) and the MSDS contains all the information required by the CPR. WHMIS Hazard Class n-controlled Page 6 / 7

16. OTHER INFORMATION Prepared By Creation Date Print Date Revision Summary Regulatory Affairs Thermo Fisher Scientific Tel: (412) 490-8929 20-Aug-2009 04-Oct-2010 "***", and red text indicates revision Disclaimer The information provided on this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guide for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered as a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other material or in any process, unless specified in the text. End of MSDS Page 7 / 7