OPERATION MANUAL FOR VP 710E-3HM SERIES ALLIGATOR TUMBLE STIRRERS *US Patent # 6,176,609

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TECHNICAL NOTE 299 OPERATION MANUAL FOR VP 710E-3HM SERIES ALLIGATOR TUMBLE STIRRERS *US Patent # 6,176,609 WARNING!!!!! Be advised that the Magnetic Tumble Stirrer has very strong magnetic fields coming from a 48 MGO Neodymium Iron Boron drive magnet. People with pacemakers should not get closer than 24 inches. Remove all magnetic influenced tools and objects from the immediate area to prevent them from being pulled onto the magnet or from striking anyone as the objects are pulled towards the magnet. Keep credit cards, watches and other magnetic sensitive items at least 24 inches from the Magnetic Tumble Stirrer s magnetic fields. Do not operate the Magnetic Tumble Stirrer in the close proximity to large pieces of aluminum or ferromagnetic material. For more information see TUMBLE STIRRER INSTALLATION section. Tumble Stirrer Aluminum Heat Block Tumble Stirrer Watlow Heat Block *Note that the above picture shows the tumble stirrer with a heat block that is sold separately 9823 Pacific Heights Boulevard, Suite T, San Diego, CA 92121 (858) 455-0643 Fax: (858) 455-0703 email: sales@vp-scientific.com

SET-UP TUMBLE STIRRER INSTALLATION Caution: Operating the Tumble Stirrer in close proximity of ferromagnetic, aluminum materials, or both, is not recommended. We recommend that the Magnetic Tumble Stirrer be installed as far away from ferromagnetic material as possible. The closer and larger the magnetic material is to the Magnetic Tumble Stirrer, the greater the torque that is required and the slower the maximum speed will be. Also large/thick aluminum structures, should be avoided. Although aluminum is not magnetic it will cause a drag on the magnetic field due to eddy currents being formed when magnetic flux lines pass through it. The eddy current effect will also cause the aluminum block to heat up. A large mass of aluminum will cause a significant drag and result in undue strain on the motor. This strain could damage the motor. This is not covered under V&P Scientific, Inc. s warranty for this product. The VP 743ABZ-L heat block is designed to specifically to be used in conjunction with the VP 710E-3HM tumble stirrer. The heat block is specially designed to significantly reduce the eddy current effect that is usually seen with aluminum. If installing away from these materials is unavoidable, test run parameters before operating instrument without supervision for long periods. The Tumble Stirrer will turn itself off when temperature of the motor exceeds its safety limit. If this happens, turn off power supply and allow motor to cool before re-starting. Modify operating parameters to prevent over-heating. Repeated over-heating could reduce product longevity. The VP 710E-3HM has a mica deck for heat protection. The mica insulation protects the magnetic cylinder and plastic box from heat damage but it is susceptible to peeling. Care must be taken when placing or removing heavy objects, such as the VP 743ABZ-L heat block which also has mica footprints, to prevent damage to the mica. We also recommend only to place or remove the heat block onto the tumble stirrer deck when it is cool as the heat can cause mica expansion, resulting in a tight fit. Mica Deck on Tumble Stirrer Mica Footprints on Heat Block OPERATION The maximum speed is set at the factory to 1000 RPM with no load and the speed control set to 100%. Because the load (magnetic resistance) will directly affect speed, it is not possible to accurately relate the dial setting to speed (RPM) in every situation. Once the optimal stirring speed has been determined, note V&P Scientific, Inc. 2 Technical Note 299 04/04/2013

the position and leave the speed control set to that speed. The stirrer can be controlled either by the rheostat control knob on the control box or by the provided computer software. Back View Manual Control Knob Connection Port to Stirrer Power Switch Front View RS-232 Connection Power Connection Port OPERATING THE TUMBLE STIRRER The optimal operating speed of the Tumble Stirrer is dependent upon the particular application to be used and needs to be empirically determined. Factors to consider in determining optimal stir speed are the application, fragility of the objects being stirred, size, shape, composition of the test tube, vial, bottle or well (polypropylene or polystyrene), depth of the microplate wells, volume and viscosity of the liquid, and the type of stir disc or bar used. In general, stirring microbial cultures works best at low speeds. Stirring to resolubilize extracts, or to stir in deep well microplates, requires high speed. The Tumble Stirrer is well suited for either of these types of applications since it can function at minimum as well as at high RPM. Again the speed of stirring needs to be determined empirically for the application. Most tumble stirring applications require a stir speed between 300 500 RPM. The VP 710E-3HM is capable of a maximum stir speed of 1000 RPM; however, note that there will be more noise at these upper speeds. This harmonic noise is normal and originates from the stepper motor. The control unit for the stirrer has an ON/OFF power switch and a rheostat speed control knob. The stirrer can be controlled either by computer software or the physical rheostat knob, but not both at the same time. A firmware download to the controller is needed to switch between manual or computer control via connecting the controller to a computer and using the software (see below). The controller connects to the computer through a RS-232 serial communication port or by USB if using the RS-232 to USB converter. Software Instructions In order to control the stirrer via software, the stirrer must be connected to a computer from the RS-232 connection. The software is provided on a USB drive and is a single executable file. Open the executable file named VP Scientific Stirrer Interface. Once the software program has opened and the stirrer is connected to the control box, turn on the power to the control box. Real-Time Control The stirrer can be directly controlled in real time under the tab Manual Control. Use the sliders to the right to control the speed and acceleration rate of the stirrer. The RUN and STOP buttons will start and stop the stirrer. Note that the acceleration cannot be set while the motor is running. V&P Scientific, Inc. 3 Technical Note 299 04/04/2013

Scheduler Control The stirrer can be setup to run at specific times, RPM, acceleration, and when to stop. This is done under the Scheduled List tab. Use the date and time fields to specify the time for a change state event (ON/OFF) Use the speed slider to specify the speed for this event Click the Add button to add the event to the list The Remove button will remove the selected event from the list The Clear button will remove all events from the list Use the acceleration slider to specify the acceleration (acceleration cannot be set for each individual event) Click the RUN button to run the scheduled program V&P Scientific, Inc. 4 Technical Note 299 04/04/2013

GENERAL PRODUCT CARE When not in use, turn the power switch off. Product Maintenance Do not place the control unit in chambers with high humidity or temperatures above 40 o C. The deck of the Tumble Stirrer is made of mica. To clean the deck use a mild detergent followed by wiping dry. The motor of the VP 710E-3HM series Tumble Stirrers is a powerful stepper motor. The motor is designed for high torque and long-term stirring applications. If technical assistance is required, contact: V&P Scientific, Inc. 9823 Pacific Heights Blvd., Suite T San Diego, CA 92121 Ph: 858-455-0643 Fax: 858-455-0703 sales@vp-scientific.com ADDITIONAL TUMBLE STIRRER SPECIFICATIONS Equipment Rating Ambient Environment: Safety Approval: 120V~, 1.6A, 60 Hz Indoor use UL Listed SAFETY PRECAUTIONS The use of Magnetic Tumble Stirrer, like that of all utilization of concentrated power, is potentially hazardous. The degree of hazard can be greatly reduced by proper design, selection, installation, and use, but all hazards cannot be completely eliminated. V&P Scientific assumes no liability for the customer s failure to comply with safety requirements and practices. V&P Scientific, Inc. 5 Technical Note 299 04/04/2013

WARRANTY V&P Scientific, Inc. warrants this product to be free from defects in material and workmanship when used under normal laboratory conditions for one year. This warranty begins from the date of delivery by V&P Scientific. In the event this product fails under normal laboratory conditions within the specified period of time because of a defect in material or workmanship, V&P Scientific will, at its option, repair or replace the product. Damage to the product caused by user negligence is not covered. Please keep the special shipping carton in case the unit needs to be shipped back to V&P Scientific. Please contact V&P Scientific at the above address for return authorization and shipping instructions. This warranty is made in lieu of other warranties expressed or implied including the warranties of merchantability and fitness for a particular purpose. V&P Scientific shall not be liable for loss or damages arising from the use of these products nor for consequential damages of any kind. V&P Scientific, Inc. 6 Technical Note 299 04/04/2013