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General Trial Information Title: RESEARCH ASSOCIATE Affiliation: NORTH CAROLINA STATE UNIVERSITY Postal Code: 27695 E-mail: TRAVIS_GANNON@NCSU.EDU Investigator: FRED YELVERTON Title: PROFESSOR Affiliation: NORTH CAROLINA STATE UNIVERSITY Postal Code: 27695 E-mail: FRED_YELVERTON@NCSU.EDU Trial Location City: WILMINGTON Trial Status: COMPLETED State/Prov.: NC Postal Code: 28411 Initiation Date: 10-1-03 Country: USA Directions: Objectives: Conclusions: Cooperator/Landowner Cooperator: Doug Lewis Country: USA Organization: Porters Neck Plantation Phone No: 910.686.7471 Address 1: 8403 Vintage Club Drive City: Wilmington State/Prov: NC Postal Code: 28411 Crop Description Crop 1: CYNDA Cynodon dactylon Variety: Tifway 419 BBCH Scale: BGRM BERMUDAGRASS Crop 2: LOLPE Lolium perenne RYEGRASS, PERENNIAL Variety: Quick Trans BBCH Scale: BGRM Planting Date: 10-15-03 Planting Method: BROADCAST Rate, Unit: 12 LB/1000FT2 Seed Bed: MOWED Soil Temperature, Unit: 66 F Soil Moisture: WET Site and Design Plot Width, Unit: 4 FT Site Type: GOLF COURSE TEE Plot Length, Unit: 6 FT Replications: 3 Study Design: Randomized Complete Block Trial Initiation Comments: Field Prep./Maintenance: Soil Description % OM: 0.27 Texture: MINERAL ph: 6.2 CEC: 3.8 Fert. Level: GOOD Moisture Conditions Overall Moisture Conditions: IRRIGATED AS NEEDED

Application Description A B C D Application Date: 10-1-03 10-10-03 12-15-03 1-5-04 Time of Day: 11-1 PM 11-12NOON 2-3:00 PM 12-1 PM Application Method: SPRAY SPRAY SPRAY SPRAY Application Timing: OCT 1 OCT 10 DEC 15 JAN 5 Application Placement: FOLIAR FOLIAR FOLIAR FOLIAR Applied By: T GANNON T GANNON T GANNON T GANNON Air Temperature, Unit: 75.5 F 70.2 F 61.3 F 81.6 F % Relative Humidity: 48 100 32 54 Wind Velocity, Unit: 1.6 MPH 1.2 MPH 1.2 MPH 2.9 MPH Dew Presence (Y/N): N Y N N Soil Temperature, Unit: 76 F 70 F 51 F 69 F Soil Moisture: GOOD WET WET GOOD % Cloud Cover: 50 100 5 10 Crop Stage At Each Application A B C D Crop 1 Code, BBCH Scale: CYNDA BGRM CYNDA BGRM CYNDA BGRM CYNDA BGRM Stage Scale Used: BBCH BBCH BBCH BBCH Stage Majority, Percent: MOWED MOWED DORMANT DORMANT Height, Unit: 0.63 IN 0.63 IN 0.63 IN 0.63 IN Crop 2 Code, BBCH Scale: LOLPE BGRM LOLPE BGRM LOLPE BGRM LOLPE BGRM Stage Scale Used: BBCH BBCH MOWED MOWED Stage Majority, Percent: N/A N/A Height, Unit: 0.63 IN 0.63 IN Application Equipment A B C D Appl. Equipment: CO2 SPRAY CO2 SPRAY CO2 SPRAY CO2 SPRAY Operating Pressure: 25 25 25 25 Pressure Unit: PSI PSI PSI PSI Nozzle Type: FLAT FAN FLAT FAN FLAT FAN FLAT FAN Nozzle Size: VS8002XR VS8002XR VS8002XR VS8002XR Nozzle Spacing, Unit: 10 INCH 10 INCH 10 INCH 10 INCH Nozzles/Row: 4 4 4 4 Boom Length, Unit: 40 INCH 40 INCH 40 INCH 40 INCH Boom Height, Unit: 10 INCH 10 INCH 10 INCH 10 INCH Ground Speed, Unit: 3 MPH 3 MPH 3 MPH 3 MPH Carrier: WATER WATER WATER WATER Spray Volume: 32.5 32.5 32.5 32.5 Volume Unit: GPA GPA GPA GPA Propellant: COMP CO2 COMP CO2 COMP CO2 COMP CO2

Crop Code LOLPE LOLPE LOLPE LOLPE LOLPE LOLPE LOLPE BBCH Scale BGRM BGRM BGRM BGRM BGRM BGRM BGRM Rating Date 10-28-03 11-12-03 12-2-03 12-15-03 1-5-04 1-22-04 2-3-04 Rating Data Type COVER COVER COVER COVER COVER COVER COVER Rating Unit % % % % % % % Days After Last Applic. 18 33 53 0 0 17 29 27 DA-A 42 DA-A 62 DA-A 75 DA-A 96 DA-A 113 DA-A 125 DA-A 1 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 38.3 a 85.0 a 78.3 a 78.3 a 73.3 a 65.0 a 65.0 a 2 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 33.3 a 86.7 a 81.7 a 78.3 a 75.0 a 66.7 a 70.0 a 3 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 38.3 a 85.0 a 76.7 a 76.7 a 75.0 a 65.0 a 68.3 a 4 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 31.7 a 86.7 a 81.7 a 78.3 a 73.3 a 66.7 a 66.7 a 5 TRANXIT 25 DF 2 oz/a OCT 1 A 33.3 a 85.0 a 76.7 a 76.7 a 71.7 a 65.0 a 68.3 a 6 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 35.0 a 86.7 a 78.3 a 80.0 a 71.7 a 66.7 a 65.0 a 7 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 30.0 a 85.0 a 71.7 a 76.7 a 71.7 a 66.7 a 66.7 a 8 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 36.7 a 86.7 a 81.7 a 80.0 a 75.0 a 68.3 a 68.3 a 9 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 30.0 a 86.7 a 78.3 a 78.3 a 75.0 a 66.7 a 66.7 a 10 CERTAINTY 75 WG 2 oz/a OCT 1 A 35.0 a 86.7 a 78.3 a 78.3 a 73.3 a 66.7 a 68.3 a 11 REVOLVER 0.19 SC 0.4 oz/1000 ft2 OCT 1 A 38.3 a 86.7 a 78.3 a 78.3 a 75.0 a 66.7 a 66.7 a 12 NONTREATED 33.3 a 85.0 a 73.3 a 78.3 a 75.0 a 66.7 a 66.7 a LSD (P=.05) 12.19 3.59 9.47 3.73 3.30 4.28 4.98 Standard Deviation 7.20 2.12 5.59 2.20 1.95 2.53 2.94 CV 20.9 2.46 7.17 2.82 2.64 3.8 4.37 Grand Mean 34.44 85.97 77.92 78.19 73.75 66.39 67.22 Bartlett's X2 9.17 1.238 14.165 2.176 0.0 0.0 2.109 P(Bartlett's X2) 0.606 0.99 0.224 0.988 1.00 0.001* 0.995 Friedman's X2 6.5 2.551 9.218 3.846 7.615 3.385 5.872 P(Friedman's X2) 0.838 0.995 0.602 0.974 0.747 0.985 0.882 Replicate F 3.309 7.592 4.200 11.286 2.200 0.762 3.453 Replicate Prob(F) 0.0554 0.0031 0.0285 0.0004 0.1346 0.4785 0.0497 Treatment F 0.536 0.493 0.927 0.766 1.650 0.436 0.759 Treatment Prob(F) 0.8578 0.8877 0.5331 0.6684 0.1528 0.9226 0.6745

Crop Code LOLPE LOLPE LOLPE LOLPE TURF TURF TURF BBCH Scale BGRM BGRM BGRM BGRM Rating Date 2-19-04 3-2-04 3-15-04 3-29-04 10-28-03 11-12-03 12-2-03 Rating Data Type COVER COVER COVER COVER QUALITY QUALITY QUALITY Rating Unit % % % % 1-9 1-9 1-9 Days After Last Applic. 45 57 70 84 18 33 53 141 DA-A 153 DA-A 166 DA-A 180 DA-A 27 DA-A 42 DA-A 62 DA-A 1 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 76.7 a 65.0 a 75.0 a 71.7 a 6.8 a 7.0 a 7.0 a 2 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 76.7 a 65.0 a 75.0 a 73.3 a 6.9 a 7.0 a 7.1 a 3 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 76.7 a 65.0 a 75.0 a 73.3 a 7.0 a 7.0 a 6.9 a 4 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 76.7 a 65.0 a 73.3 a 70.0 a 6.7 a 7.0 a 7.2 a 5 TRANXIT 25 DF 2 oz/a OCT 1 A 73.3 a 63.3 a 73.3 a 70.0 a 6.8 a 7.0 a 6.8 a 6 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 75.0 a 63.3 a 73.3 a 70.0 a 7.0 a 7.0 a 7.0 a 7 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 75.0 a 63.3 a 71.7 a 70.0 a 6.6 a 7.0 a 6.8 a 8 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 75.0 a 65.0 a 75.0 a 73.3 a 6.8 a 7.0 a 7.3 a 9 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 78.3 a 66.7 a 75.0 a 71.7 a 6.7 a 7.0 a 7.0 a 10 CERTAINTY 75 WG 2 oz/a OCT 1 A 76.7 a 65.0 a 73.3 a 71.7 a 6.8 a 7.0 a 7.1 a 11 REVOLVER 0.19 SC 0.4 oz/1000 ft2 OCT 1 A 75.0 a 66.7 a 73.3 a 71.7 a 6.9 a 7.0 a 6.8 a 12 NONTREATED 73.3 a 65.0 a 73.3 a 70.0 a 6.8 a 7.0 a 6.6 a LSD (P=.05) 4.87 3.10 4.81 4.15 0.39 0.00 0.57 Standard Deviation 2.88 1.83 2.84 2.45 0.23 0.00 0.34 CV 3.8 2.82 3.85 3.43 3.38 0.0 4.85 Grand Mean 75.69 64.86 73.89 71.39 6.81 7.0 6.96 Bartlett's X2 4.903 0.0 2.522 2.469 4.248 0.0 7.192 P(Bartlett's X2) 0.843 1.00 0.925 0.982 0.894. 0.783 Friedman's X2 5.128 3.962 2.821 7.077 6.846 0.0 8.936 P(Friedman's X2) 0.925 0.971 0.993 0.793 0.811 1.00 0.628 Replicate F 9.153 1.453 2.406 11.232 5.107 0.000 6.710 Replicate Prob(F) 0.0013 0.2555 0.1135 0.0004 0.0151 1.0000 0.0053 Treatment F 0.817 1.113 0.438 0.968 1.009 0.000 0.913 Treatment Prob(F) 0.6249 0.3967 0.9216 0.5007 0.4700 1.0000 0.5447

Crop Code TURF TURF TURF TURF TURF TURF TURF BBCH Scale Rating Date 12-15-03 1-5-04 1-22-04 2-3-04 2-19-04 3-2-04 3-15-04 Rating Data Type QUALITY QUALITY QUALITY QUALITY QUALITY QUALITY QUALITY Rating Unit 1-9 1-9 1-9 1-9 1-9 1-9 1-9 Days After Last Applic. 0 0 17 29 45 57 70 75 DA-A 96 DA-A 113 DA-A 125 DA-A 141 DA-A 153 DA-A 166 DA-A 1 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 7.2 a 7.0 a 6.0 a 6.0 a 6.0 a 6.0 a 7.0 a 2 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 7.2 a 7.0 a 6.0 a 6.0 a 6.0 a 6.0 a 7.0 a 3 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 7.1 a 7.0 a 6.0 a 6.0 a 6.0 a 6.0 a 7.0 a 4 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 7.2 a 7.0 a 6.0 a 6.0 a 6.0 a 6.0 a 7.0 a 5 TRANXIT 25 DF 2 oz/a OCT 1 A 7.1 a 7.0 a 6.0 a 6.0 a 6.0 a 6.0 a 7.0 a 6 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 7.3 a 7.0 a 6.0 a 6.0 a 6.0 a 6.0 a 7.0 a 7 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 7.2 a 7.0 a 6.0 a 6.0 a 6.0 a 6.0 a 6.8 a 8 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 7.3 a 7.0 a 6.0 a 6.0 a 6.0 a 6.0 a 7.0 a 9 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 7.2 a 7.0 a 6.0 a 6.0 a 6.0 a 6.0 a 7.0 a 10 CERTAINTY 75 WG 2 oz/a OCT 1 A 7.2 a 7.0 a 6.0 a 6.0 a 6.0 a 6.0 a 7.0 a 11 REVOLVER 0.19 SC 0.4 oz/1000 ft2 OCT 1 A 7.2 a 7.0 a 6.0 a 6.0 a 6.0 a 6.0 a 7.0 a 12 NONTREATED 7.2 a 7.0 a 6.0 a 6.0 a 6.0 a 6.0 a 7.0 a LSD (P=.05) 0.16 0.00 0.00 0.00 0.00 0.00 0.14 Standard Deviation 0.09 0.00 0.00 0.00 0.00 0.00 0.08 CV 1.32 0.0 0.0 0.0 0.0 0.0 1.19 Grand Mean 7.17 7.0 6.0 6.0 6.0 6.0 6.99 Bartlett's X2 0.0 0.0 0.0 0.0 0.0 0.0 0.0 P(Bartlett's X2) 0.001*...... Friedman's X2 3.692 0.0 0.0 0.0 0.0 0.0 0.846 P(Friedman's X2) 0.978 1.00 1.00 1.00 1.00 1.00 1.00 Replicate F 12.158 0.000 0.000 0.000 0.000 0.000 1.000 Replicate Prob(F) 0.0003 1.0000 1.0000 1.0000 1.0000 1.0000 0.3840 Treatment F 0.842 0.000 0.000 0.000 0.000 0.000 1.000 Treatment Prob(F) 0.6034 1.0000 1.0000 1.0000 1.0000 1.0000 0.4767

Crop Code TURF LOLPE LOLPE LOLPE LOLPE LOLPE LOLPE BBCH Scale BGRM BGRM BGRM BGRM BGRM BGRM Rating Date 3-29-04 12-2-03 12-15-03 1-5-04 1-22-04 2-3-04 2-19-04 Rating Data Type QUALITY PHYTO PHYTO PHYTO PHYTO PHYTO PHYTO Rating Unit 1-9 % % % % % % Days After Last Applic. 84 53 0 0 17 29 45 180 DA-A 62 DA-A 75 DA-A 96 DA-A 113 DA-A 125 DA-A 141 DA-A 1 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 7.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 2 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 7.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 3 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 7.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 4 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 7.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 5 TRANXIT 25 DF 2 oz/a OCT 1 A 7.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 6 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 7.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 7 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 7.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 8 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 7.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 9 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 7.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 10 CERTAINTY 75 WG 2 oz/a OCT 1 A 7.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 11 REVOLVER 0.19 SC 0.4 oz/1000 ft2 OCT 1 A 7.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 12 NONTREATED 7.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a 0.0 a LSD (P=.05) 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Standard Deviation 0.00 0.00 0.00 0.00 0.00 0.00 0.00 CV 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Grand Mean 7.0 0.0 0.0 0.0 0.0 0.0 0.0 Bartlett's X2 0.0 0.0 0.0 0.0 0.0 0.0 0.0 P(Bartlett's X2)....... Friedman's X2 0.0 0.0 0.0 0.0 0.0 0.0 0.0 P(Friedman's X2) 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Replicate F 0.000 0.000 0.000 0.000 0.000 0.000 0.000 Replicate Prob(F) 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Treatment F 0.000 0.000 0.000 0.000 0.000 0.000 0.000 Treatment Prob(F) 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000

Crop Code LOLPE LOLPE LOLPE BBCH Scale BGRM BGRM BGRM Rating Date 3-2-04 3-15-04 3-29-04 Rating Data Type PHYTO PHYTO PHYTO Rating Unit % % % Days After Last Applic. 57 70 84 153 DA-A 166 DA-A 180 DA-A 1 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 0.0 a 0.0 a 0.0 a 2 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 0.0 a 0.0 a 0.0 a 3 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 0.0 a 0.0 a 0.0 a 4 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 0.0 a 0.0 a 0.0 a 5 TRANXIT 25 DF 2 oz/a OCT 1 A 0.0 a 0.0 a 0.0 a 6 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 0.0 a 0.0 a 0.0 a 7 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 0.0 a 0.0 a 0.0 a 8 TRANXIT GTA 25 DF 1 oz/a OCT 1 A 0.0 a 0.0 a 0.0 a 9 TRANXIT GTA 25 DF 2 oz/a OCT 1 A 0.0 a 0.0 a 0.0 a 10 CERTAINTY 75 WG 2 oz/a OCT 1 A 0.0 a 0.0 a 0.0 a 11 REVOLVER 0.19 SC 0.4 oz/1000 ft2 OCT 1 A 0.0 a 0.0 a 0.0 a 12 NONTREATED 0.0 a 0.0 a 0.0 a LSD (P=.05) 0.00 0.00 0.00 Standard Deviation 0.00 0.00 0.00 CV 0.0 0.0 0.0 Grand Mean 0.0 0.0 0.0 Bartlett's X2 0.0 0.0 0.0 P(Bartlett's X2)... Friedman's X2 0.0 0.0 0.0 P(Friedman's X2) 1.00 1.00 1.00 Replicate F 0.000 0.000 0.000 Replicate Prob(F) 1.0000 1.0000 1.0000 Treatment F 0.000 0.000 0.000 Treatment Prob(F) 1.0000 1.0000 1.0000 10-8-03 - Unable to apply treatments today due to 2 inches of rainfall. Trial Comments 10-10-03 - Treatments that were to be applied 10-8 were applied today. Trial area was extremely wet and it was misting lightly 1 hour prior to applying treatments. 10-15-03 - Trial area was lightly verti cut, mown to 0.63 inch and clippings were blown off. Trial was overseeded with Quick Trans perennial ryegrass (Lot# B10-3-QT-1) which was purchased from Southern Seed. Seedling (1 leaf) annual bluegrass is beginning to appear in trial area.

10-28-03 - Trial was evaluated for perennial ryegrass cover and turf quality. Select treated plots exhibit reductions in perennial ryegrass cover. 11-12-03 - Trial was evaluated for perennial ryegrass cover and turf quality. 0.75 lb N per 1000 ft2 was applied to trial area. 12-2-03 - Trial was evaluated for perennial ryegrass cover, phytotoxicity, and turf quality. Ryegrass is thinner in select areas resulting in decreased ryegrass cover ratings, not neccesarily a result of treatments. Very little annual bluegrass is present in turf canopy. 12-15-03 - Trial was evaluated for perennial ryegrass cover, phytotoxicity, and turf quality. Annual bluegrass is beginning to be more prevalent in non-overseeded areas outside of test area. 1-5-04 - Trial was evaluated for perennial ryegrass cover, phytotoxicity, and turf quality. Annual bluegrass is beginning to be more prevalent in non-overseeded areas outside of test area. 1-22-03 - Trial was evaluated for perennial ryegrass cover, phytotoxicity, and turf quality. Annual bluegrass is beginning to be more prevalent in non-overseeded areas outside of test area. 2-3-04 - Trial was evaluated for perennial ryegrass cover, phytotoxicity, and turf quality. Annual bluegrass is beginning to be more prevalent in non-overseeded areas outside of test area. Cold periods have reduced overall turf quality. 0.75 lb N per 1000 ft2 was applied to trial area. 2-19-04 - Trial was evaluated for perennial ryegrass cover, phytotoxicity, and turf quality. Additional annual bluegrass is beginning to be more apparent within the trial area. Outside trial area in non-overseeded areas, a few (<10%) of the annual bluegrass plants have emerging seedheads. No seedheads are present in overseeded trial area at this point. 3-2-04 - Trial was evaluated for perennial ryegrass cover, phytotoxicity, and turf quality. Currently, there are no seedheads present in overseeded trial area, thus unable to rate for annual bluegrass control at this time. 3-29-04 - Trial was evaluated for perennial ryegrass cover, phytotoxicity, and turf quality. Poa annua is not dense enough in trial area to evaluate. 4-14-04 - Poa annua is not dense enough in trial area to evaluate, trial concluded.

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