Statewide Washington Oncorhynchus mykiss SNP baseline development Todd R. Seamons and Kenneth I. Warheit Washington Department of Fish and Wildlife Science Division
Partners/collaborators/funders/sample Maureen Small WDFW Todd Kassler WDFW Bonneville Power Administration Douglas County PUD Colville Tribe Seattle City Light Spokane Tribe Yakama Nation NOAA Upper Skagit Indian Tribe Bill McMillan WFC Skagit River system Cooperative collectors Holly McLellan Colville Tribe Chuck Lee WDFW Gabe Temple WDFW Chris Frederiksen Yakama Nation Charlie Snow WDFW Ben Goodman WDFW Steelhead Trout Club of Washington University of Washington Jeff Stephenson CRITFC WDFW general fund Quinault Tribe WDFW regional staff
WA State Oncorhynchus mykiss genetic resources Non native hatchery rainbow trout Two lineages of native steelhead/rainbow trout Hatchery broodstocks Wild anadromous/resident populations Rainbow trout isolated upstream of barriers 189 SNP loci (+ 3 loci that identify O. clarki and hybrids)
Two major lineages of native O. mykiss Interior redband O. mykiss gairdneri Summer run Resident Up and downstream of natural barriers Coastal O. mykiss irideus Summer run Winter run Resident Up and downstream of natural barriers
Coastal lineage Interior Redband lineage CA hatchery trout
Primary goal: collections from all DIPs, from all DPS
DPS Summerrun Winterrun Current genetic coverage Puget Sound 5 26 77% Olympic Peninsula 7 24 19% Southwest Washington 2 17 5% Lower Columbia River 5 14 6% Middle Columbia River 9 0 44% Upper Columbia River 4 0 50% Snake River 4 0 0%
Native hatchery broodstocks Steelhead Chambers early winter South Puget Sound Skamania early summer Lower Columbia Washougal River Tribal/Federal hatchery early winters North coast Cook Creek/Quinault River Other locally derived Resident rainbow trout Phalon Lake Redband rainbow trout Upstream of Grand Coulee Dam Ross Lake Upper Skagit River
Native hatchery broodstocks Stock Broodstock N Current genetic coverage Cook Creek early winters 5 100% Chambers early winters 22 36% Skamania early summers 11 36% Locally derived 26 12% native resident rainbows 2 50%
Non native hatchery rainbow trout Three strains currently propagated, all of N. California ancestry Mt. Whitney Goldendale Spokane S. Tacoma Also purchase Troutlodge Broodstock kept distinct space and time but strains all reared and stocked widely
S. Tacoma strain Troutlodge strain Spokane strain Strains are very different despite Goldendale strain common ancestry Among strains average Fst = 0.16 Mt. Whitney strain
Lower Columbia (Washougal R.) Skamania H. axis 1 = 4.6% axis 2 = 3.2% N Coastal Puget Sound Chambers Creek H. S
Twisp River Upper Columbia? N Wenatchee S Yakima
Interior Redband Coastal CA hatchery trout
Avg Fst = 0.13 Interior Redband Avg Fst = 0.31 Coastal Avg Fst = 0.28 CA hatchery trout
What is the ancestry of S.F. Skykomish rainbow trout? Puget Sound winters Puget Sound summers Skamania early summers S.F. Skykomish test samples
What is the ancestry of S.F. Skykomish rainbow trout? Puget Sound winters Puget Sound summers Skamania early summers S.F. Skykomish test samples Looks like mostly introduced Lower Columbia (Skamania early summers) Upper Tye River
What is the ancestry of S.F. Skykomish rainbow trout? CA hatchery trout Puget Sound winters S.F. Skykomish test samples Naches Interior Redband Skamania early summers Upper Tye River appear to be Interior Redband lineage
Introgression with CA hatchery lineages in some upper Columbia resident Redband rainbow populations Coastal CA hatchery trout Interior Redband rainbow trout Interior Redband
Expected Improvements over previous work Better spatial coverage Especially once we get collections genotyped Eventual inclusion of all hatchery stocks Better coverage of resident populations Better statistical power E.g., assignment tests