To provide barriers against skin, mucous membrane, and respiratory exposure to infectious agents to prevent the spread of contamination

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Criteria for Selection and Use of Personal Protective Equipment. Selection Objectives Ephy Khaemba International Livestock Research Institute Laboratory Management and Equipment Operations Workshop Use & Limitations Donning / Doffing Common Applications Why Use Personal Protective Equipment? Introduction To provide barriers against skin, mucous membrane, and respiratory exposure to infectious agents to prevent the spread of contamination Why Use PPE? Controlling exposure to hazards Elimination or substitution Engineering controls Work practice controls Personal protective equipment last resort (PPE) Routes of Exposure Forms Of Contaminants Inhalation Ingestion PARTICULATES Dust Mist GAS / VAPOUR Gas Absorption Puncture Fume Fibre Vapour 1

Class Activity: Identifying Types of PPE to Protect Against Exposure by Different Routes Requirements for PPE Program Ingestion Percutaneous Inhalation Contact Risk assessment Hazard identification Selecting the PPE Training Employees in Proper Use of PPE Example of a risk matrix for Biological Harzard Type of Form of Biohazard Known Routes of Known How is the How Biohazard Exposure Infective Dose Biohazard frequently is of Biohazard Handled in the Biohazard the Lab Handled Bacteria Culture, live Contact with Pipetted >5 times/day Virus Frozen Mucous Handled 2-4 times/day Prion Lyophilized Members/Eyes with Sharps once daily Human Fixed Inhalation of Centrifuged up to 4 Pathogen DNA Aerosols Poured times/week Animal RNA Ingestion Inoculated 1 time/week Pathogen- Recombinant DNA Contact with nonintact skin Used to 2-3 with Loop Non- Recombinant RNA Zoonotic Diagnostic Parenteral prepare slides times/month Animal Samples Inoculation Cultures monthly Pathogen- Blood Other Grown 2-4 times/year Zoonotic Serum Infected 5-11 times/year Other Sputum Animal less than once a Feces Other year CFS other Tissue(type) Environmental Source Other Quantity of Biohazard Handled at One Time ul/g quantities >1ml/g 1ml/g-100ml/g 100ml/g- 1000ml/g <1 litre/kilogram unknown How to select your PPE Familiarise with potential hazards and types of PPE available 1. Anticipate exposure 2. Durability and appropriateness of PPE to the task Consider the hazard association with Environment Select PPE that ensures greater level of protection than minimum requirement. Fit the worker with PPE and give instructions on use and care. Make workers aware of limitation of PPE. If several different type of PPE are worn together, make sure they are compatible Training Employees In proper use of PPE Why PPE is necessary. When PPE is necessary. What PPE is necessary. How to properly put on, take off, adjust and wear the PPE. The limitations of the PPE. Proper care, maintenance, useful life and disposal of PPE. 2

Personal Protective Equipment Policies and Procedures Policy and Procedure should include: 1. Selection 2. Training 3. Fit testing 4. Use and Maintenance Gloves Gloves help protect you when directly handling potentially infectious materials or contaminated surfaces. Wear gloves when directly handling potentially infectious materials or in contact with contaminated surfaces. Vinyl, latex, or nitrile gloves may be worn. (there are many other types of gloves other than the ones listed here) Change gloves when visibly soiled, torn or punctured. Wash hands upon removing gloves. Gloves do not replace the need for excellent hand washing! http://www.bestglove.com Gloves Purpose is to protect from infectious agents/chemicals being transmitted/absorbed via hands Body fluids, mucous membranes non-intact skin Contaminated equipment and surfaces Considerations Fit (tight fitting or loose fitting) Material Sterile, non sterile Use and reuse, disposable versus reusable Types of Protective Gloves Type of chemicals handled. Nature of contact (total immersion, splash, etc.). Duration of contact. Area requiring protection (hand only, forearm, arm). Grip requirements (dry, wet, oily). Thermal protection. Size and comfort. Abrasion/resistance requirements. Gloves made from a wide variety of materials are designed for many types of workplace hazards. In general, gloves fall into four groups: Insulating rubber gloves (See 29 CFR 1910.137 and the following section on electrical protective equipment for detailed requirements on the selection, use and care of insulating rubber gloves). Gloves made of leather, canvas or metal mesh; Fabric and coated fabric gloves; Chemical- and liquid-resistant gloves; Example: Doffing Gloves 1. 2. Gowns Gowns help protect you from the contamination of clothing with potentially infectious material. Wear a gown when contamination of clothing with potentially infectious material is possible. Your gown should fully cover the torso, fit close to the body and cover the arms to the wrists. Choose a gown appropriate to the situation: Disposable vs re-useable (requires laundering). Fluid-resistant vs non fluid-resistant. Sterile vs clean. 3. 4. 3

O.R. Gown Full Barrier Front Tyvek Suits, Coveralls Protect your skin and clothing when working with dirt, paint, solvents, chemicals, oil, grease. These multipurpose suits are durable and can be worn over and over. raised neckband, colour coded to identify size GORE LP Surgical Fabric protection on gown front and on sleeves from 4" above elbow to knitted cuff and yolk to hem three sets of inside centre back tape ties overlap back for better coverage outside tie closure at waist seam sealed sleeves Scrubs Head and body protection in a disposable coverall with elasticized cuffs to protect arms and legs. The durable Tyvek fabric is hard to tear or puncture, yet it functions as a breathable membrane that allows body heat and sweat to escape while preventing chemicals, paints and particles from getting in. (There are many other types of coveralls made of cloth, heavy PVC/Poly, etc..) Shoe and Head Covers Masks and Respirators Shoe covers Wear shoe covers to provide a barrier against possible exposure to airborne organisms or contact with a contaminated environment. Head covers Wear head covers to protect the hair and scalp from possible contamination when sprays or airborne exposure is anticipated. Surgical masks help protect your nose and mouth from splattered body fluids(such as blood, respiratory secretions, vomit, urine or feces). (a surgical mask is not a respirator) Respirators filter the air before you inhale it. Respirators Air Purifying Respirators Respirators filter the air you breathe to help protect you from microorganisms including bacteria and many viruses. Types of respirators include: Disposable Respirators (includes N95, N100) Powered Air Purifying Respirator (PAPR) Self-Contained Breathing Apparatus (SCBA) Respirators Non-powered ( Negative Pressure ) Powered ( Positive Pressure or PAPR ) Air-Mate TM HEPA PAPR System R-Series TM PAPR System 4

Negative Pressure Air Purifying Respirator HOW IT WORKS By inhaling, a negative pressure is created in the respirator. Ambient air flows through a filter or cartridge, which removes the contaminants. The clean air continues into the respirator and then the lungs. Positive Pressure Air Purifying Respirator (A.K.A. Powered Air Purifying Respirators (PAPRs)) The motor/blower unit pulls the ambient air through a filter or cartridge, which removes the contaminants, and then forces the purified air into the breathing zone. Motor/ Blower Particle Filter Gas/Vapour Media Filter Gas/Vapour Media Fit Testing Methods Scenes from a Respirator Fit Test QUALITATIVE: Subjective pass/fail method. Sensory response of the test subject to a test agent. QUANTITATIVE: Objective test. Number generated. Factors Affecting Face Seal Facial hair Weight loss/gain Wrinkles, scars, acne, make-up Facial structure Dentures NIOSH Particulate Filter Classifications N-Series: Not for oil Approved for non-oil particulate contaminants Use until increased breathing resistance or damaged (In Health Care settings, the infection control practices take precedence) R-Series: Resistant to oil Approved for all particulate contaminants Time restriction of 8 hours when oils are present P-Series: Oil Proof Approved for all particulate contaminants Manufacturer s time use restrictions apply 5

NIOSH Particulate Filter Classifications Limitations of Air Purifying Respirators Not for use in atmospheres containing less than 19.5% oxygen Not for use in Immediately Dangerous to Life and Health (IDLH) atmospheres Not for use when concentration exceeds Assigned Protection Factor (APF) X Exposure Limit Do not use with facial hair, or other conditions that interfere with the seal between the face and the respirator (N100, ½ or full face tight fitting respirator) Do not alter, abuse, or misuse the respirator Respiratory Protective Equipment Spectrum Any Questions? Surgical Mask Half Facepiece, Filtering Facepiece or Maintenance- Free Full-face Respirator Powered- Air Purifying Respirator Air- Line Self- Contained Breathing Apparatus Protection 6