Faculty/School: Psychology Initial Issue Date: 27/08/2015 Next Review Date: 27/08/2017 Risk Assessment Reference Number: LCMS_0001.

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Use this form to assist you to complete risk assessments for hazardous activities and processes. Any serious or ongoing hazards should be reported via RiskWare to ensure that appropriate corrective actions are tracked and completed. Faculty/School: Psychology Initial Issue Date: 27/08/2015 Next Review Date: 27/08/2017 Risk Assessment Reference Number: LCMS_0001 Risk Assessment Name: Prepared by: Operation of SHIMADZU LC/MS/MS 8030 Triple Quadrupole Liquid Chromatography Mass Spectrometer Jordyn Stuart Responsible supervisor/s: Iain McGregor Identify the activity and the location Activity or process: Operation of SHIMADZU LC/MS/MS 8030 Triple Quadrupole Liquid Chromatography Mass Spectrometer Identify who may be at risk This may include fellow workers, students, visitors, contractors and the public Persons at risk: Any worker or student who uses the machine- ONLY TRAINED PERSONALE SHOULD BE USING THE MACHINE Location: Badham Building A16 Level 2 Room 247 Risk assessment team (Who was consulted?): Jordyn Stuart John Holden List of Legislation, Code of Practice, Australian Standards, Guidance Materials used to determine control measures Risk Assessment Methodology Assessing the risk is a brainstorming exercise, which is most effectively carried out in a team environment with the people required to complete the activity or process. Most activities or processes are broken down into a variety of separate tasks. For each task, consider the hazards, the potential harm or negative outcomes and the conditions required for those negative outcomes to occur. Whenever assessing the health and safety risks associated with a task, always consider the following primary risk factors. The physical activities required to complete the task e.g. repetitive movement, high force, physical exertion, awkward posture The work environment e.g. lighting, work layout, traffic, thermal comfort, working in isolation The nature of the hazard itself e.g. working with chemicals, microorganisms, radiation, machinery, potentially violent clients The individual workers involved, e.g. level of training, skills, experience, health, age, physical capacity The information gathered from the risk assessment process must be used to develop a Safe Work Procedure (SWP).

Task or scenario Changing pump oil Hazard/s Burns/Slip Associated harm, e.g. what could go wrong? When the pump has been running for a number of days it gets very hot and can therefore burn you. Also if oil is spilled then it causes the floor to be very slick and could cause someone to fall. Existing Risk Controls Catch Tray to prevent leaking oil from the ground and pump turned off before changing oil Current risk rating Use the Risk Matrix Any additional controls are required? 1 Residual risk rating Use the Risk Matrix Changing gas cylinder Changing ion source Violent rupture Gas cylinder leaking Burns/ electric shock Potential violent rupture if knocked over when unsecured or dropped while moving Could cause asphyxiating atmosphere leading to drowsiness, unconsciousness or death High voltage is used as well as high heat so both must be turned off before opening the source window. Only trained personnel should handle gas cylinders Gas regulators to be checked regularly Check for leaks by using soapy water to look for bubbles Signs on machine warn of voltage and proper training to know how to use program Medium No Medium Making mobile phase Replacing mobile phase bottle Chemical burn or exposure Fire Spill Could spill an irritant on your skin Inhaling some chemicals can be dangerous Some chemicals are flammable Some are acidic The mobile phase dock is tall and therefore to reach it may have to reach a chemical filled bottle over their head and could drop it or spill it on them. We make all mobile phases in the fume hood to prevent inhalation, wear gloves and lab coat to prevent spills on skin, and chemicals are stored in proper places A foot stool is provided if cannot reach the mobile phase dock. Mobile phase bottles have lids that can be secured on to prevent spilling if someone were to tip the bottle on themselves. Also bottles are made out of a coated glass to prevent breaks in case dropped. Medium No Medium 1 Always consider whether or not it is possible to eliminated the hazard or hazardous task altogether. If this is not possible, refer to the hierarchy of risk controls.

Sample prep or disposing of samples Glass breaking If you drop an autosampler there is potential they could break and therefore be a cut hazard We use racks to try and prevent he ability to knock over the glass consumables Replacing broken autosampler needle Changing columns Stabbed with a needle Chemicals spill/skin irritaion Replacing needle could stab you if not trained in how to replace it If there are leaks throughout the HPLC system they could leak on your skin if not wearing protective gear Only trained personnel is able to do this (usually contracted out). Also needle only functions when the autosampler door is closed to prevent other stabbing. Must wear gloves, goggles and lab coat when in use. Turn the pumps off before attempting to stop the leak. Page 3

Implementation of Additional Risk Controls Additional controls needed Resources required Responsible person Date of implementation RiskWare Reference Write the Safe Work Procedure (SWP) Time (approx 1 hour) Supervisor N/A Train workers to complete process in accordance with SWP Time supervisor and workers Supervisor N/A List emergency controls for how to deal with fires, spills or exposure to hazardous substances and/or emergency shutdown procedures Fire- There are two fire extinguishers located by the sink next to the door, a Dry Chemical (white band) and Carbon Dioxide (black band). A fire blanket is also available. Except for fires within the fume hood, the fire extinguisher of choice is Carbon Dioxide. If fire is in fume hood then close the sash, hit the red power off button and if appropriate use the Dry Chemical fire extinguisher. If it cannot be contained hit the emergency power off button and evacuate. Spills/exposure- There is a spill kit under the sink near the door as well as an emergency shower and eye wash station. There is an electrical shut off button that shuts off all power to the room 247 if there is an emergency that is immediate. If there is time to manually power down the machine. REVIEW Scheduled review date 1 year 2 years 3 years Are control measures in place (YES/NO) Are controls eliminating or minimizing the risk (YES/NO) Are there any new problems with the risk (YES/NO) Reviewed by: Actual Review date:

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Risk Matrix. RISK ASSESSMENT FORM