Real-time gas analysis and software for the management of bioprocessing
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- Gerald Terry
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1 Understanding bioprocesses from the lab to the production understanding bioprocesses Real-time gas analysis and software for the management of bioprocessing
2 Understanding bioprocesses for more than 15 years The founders and managing directors of BlueSens Dr. Udo Schmale (left) and Dr. Holger Müller (right) BlueSens was founded 2001 in Germany and develops and manufactures analyzers and software for bioprocess applications. In 2015 the BlueSens Corporation was founded in Illinois. The motivation of BlueSens is to provide instruments and software so that their customers gain comprehensive understanding of their bioprocesses to get a maximum optimized process for maximum yield at maximum quality. To achieve that, BlueSens is offering a variety of gas analyzers to monitor key parameters of bioprocesses. In general, BlueSens gas analyzers are installed directly in the process and measure in real-time gases such as CH 4, O 2 /CO 2, CO, H 2 or Ethanol. Each sensor is individually tested and adjusted in calibration. BlueSens has developed a unique calibration process guaranteeing that every sensor is a hand-made unique and individually tested device completely Made in Germany! Products Gas Sensors and Analysis Systems BlueVary BlueInOne Yieldmaster BlueVCount BCpreFerm BCP sensors Software BlueVIS Key facts BlueSens No sampling real-time measurements Faster reaction times avoid extended run times for sampling just as any contamination. Gain scale up and down parameters Gain important parameters and measure accurately no matter if lab or production scale. Quality made in Germany All BlueSens sensors are developed, produced, calibrated and assembled in Germany under fair production conditions. Service and Maintenance Support 2
3 BlueVary: Maintenance free & flexible Key facts BlueVary Maintenance free No need for annual maintenance. Performing our established one-point calibration regularly is all you have to do. No specific training easy to install and handle Install and calibrate it yourself. PC housing and various connections guarantee easy handling. EtOH O2 CO2 CH4 H2 Maximum flexibility Combine two gases for parallel measuring (CO 2, O 2, H 2, CH 4, EtOH). Use it also for biogas applications. CO2 O2 H2 CH4 EtOH BLUEVARY.BLUESENS.COM The basis station with three plug-in positions and an integrated data display operated by control buttons. Link to BlueVIS and other third party software. Watch animation online: Three plug-in-cartridges: two for gases and one obligatory humidity and/or pressure cartridge. Connectable to any hose/ tube or pipe use it on your fermenter of choice. 3
4 BlueInOne: Combined CO 2 /O 2 sensor Key facts BlueInOne in 4 1 One device for the parallel measurement O 2 and CO 2, humidity and pressure. No minimal gas flow needed. Fluctuating pressure/humidity are autocompensated. Safe time and money The reasonably priced BlueInOne helps you to understand your process in real-time to save time. CER, OUR, RQ Biomass Growth rate µ max, X s, Y xs Perfect gas analyzer for microbial processes One analyzer per process means continuous real-time measurement without interruptions. BLUEINONE.BLUESENS.COM 4
5 BlueVCount: Volumetric flow-measurement 1 ml Key facts BlueVCount More process information Leads to less number of experiments and optimized yields. Less maintenance No water or oil refill necessary. bar C Auto compensation Shows standard volume because of integrated pressure and temperature compensation. BLUEVCOUNT.BLUESENS.COM 5
6 Yieldmaster: Gold Standard of BMP test systems Key facts Yieldmaster Modular Bio-Methane Potential (BMP) evaluation system Microprocessor controlled infrared-gas sensors, precision volumetric flow-meters BlueVCount and BlueVIS software. EtOH O2 CO2 CH4 H2 No gas treatment/filtering needed The analyzers might either be positioned directly at the headspace or in the off-gas line of any fermenter. Maximum flexibility Detection of other important gases besides CH 4 possible. BMP Bio-Methane Potential YIELDMASTER.BLUESENS.COM 6
7 BC pre Ferm BCpreFerm: OUR, CER, RQ for shake flasks Key facts BCpreFerm System for process optimization Designed for scale up from flasks up to large-scale fermenters. up to 12 Up to 12 gas sensors The gas sensors monitor vital parameters both on fermenters as well as on flasks. No limitations Dedicated process optimization without limitations (e.g. oxygen, nutrients etc.) BCPREFERM.BLUESENS.COM OUR, CER, RQ BCP series BCP sensors: Easy in-situ analysis Key facts BCP sensors EtOH O2 CO2 CH4 H2 Wide choice Each sensor is for a single gas: H 2, CO 2, CO, Ethanol, O 2, CH 4. BCP.BLUESENS.COM Different housing All BCP sensors are avaiable with PA or Aluminium housing (IP65) 7
8 Blue BlueVIS: One software for all Key facts BlueVIS software Parallel tracking of important process parameters Monitoring, acquisition and integration of data from all components and for process control in real-time. Digital and anlog data input implemented Analog devices can be connected via analog/digital converter. Open to other systems and brands Integrated OPC server/client for data exchange to other systems and programs. SENSORS & PROBES MEASUREMENT PROVIDES LOGGING & VISUALIZATION BlueVIS Calculates OUR, CER, RQ, growth rate and biomass*, offers math sensors and connects to MATLAB. * by EXPUTEC soft sensors BLUEVIS.BLUESENS.COM BIOPROCESS OPTIMIZES & CONTROLS PROCESS Blue SOFTWARE CALCULATES & PROCESSES PROCESS PARAMETERS 8
9 Blue BlueVIS: Take what you have Digital mass flow controllers for gas input control BlueInOne gas analyzer for monitoring exhaust gas: O 2, CO 2 Trace C2 Control for: glucose, lactate, methanol/ ethanol in the liquid phase Agitator control Pumps for feeding and ph control Integrated math sensors, connection to MATLAB via integrated OPC server/client ph, DO, and other probes for liquid phase measurements Most fermenter vessels and heating/ cooling systems digital analog Your advantages: Re-use equipment you already have. Or use BlueVIS to set-up your own bioprocessing system. You don t have equipment yet? Ask us for a complete pre-assembled system. Connections: Sensors and probes Measurement of Phase Connection type to BlueVIS BlueSens O 2, CO 2, H 2, CH 4, humidity pressure, Ethanol gas RS 485/USB TRACE Analytics glucose, lactate, methanol, and ethanol liquid Ethernet Hamilton probes ph, DO and more liquid RS485/USB, Bluetooth Soft sensors OUR,CER, RQ, growth rate, biomass various Internal calculations Analog sensors various various Connection via analog/digital converter 9
10 Everything at a glance: product overview Sensor CO2** CH4** CO EtOH** BlueInOne Ferm Measuring range 0 10 Vol. % Vol. % 0 30 Vol. % Vol. % Vol. % CO 2, Vol. % O Vol. % Vol. % 0 25 Vol. % CO 2, Vol. % O Vol. % Vol. % CO 2, 1 50 Vol. % O Vol. % CO 2, 1 50 Vol. % O 2 Measuring principle Infrared, dual wavelengths Infrared, dual wavelengths (CO 2) / ZrO 2 (O 2) Accuracy Long-term stability 2 < ± 0.2 % FS* ± 3% reading < ± 2% reading / year Lifetime sensor element > 3 years Approx. 3 years (CO 2), 15,000 operating hours (O 2) Housing Aluminum, IP 65 Dimension (WxDxH) mm Dimension (WxDxH) inch Weight Housing PA6 Dimension (DxH) mm Dimension (DxH) inch Weight Material in contact with gas 900 g (1.98 lb) 80 x g (1.98 lb) 80 x g (1.98 lb) 80 x g (6.61 lb) 80 x x 150 x x 5.91 x g (8.82 lb) Not available Steel / Sapphire / Viton / PTFE Stainless steel / Viton / Sapphire / PTFE / Polymer H.L. / Nitrile Connection 6 G 1¼, GL 45, Tri-Clamp, hose connection 4-12mm etc. ¼ 1 ¼ 6, hose connection 4-12mm etc. General Operating temperature Max C / F C / F Storage temperature 0 60 C / F Pressure range (absolute): bar / psi 6 absolute pressure Storage humidity 75% RH noncondensing > 75% RH noncondensing Operating humidity % RH % RH Integrated humidity compensation Power supply (max.) 12 or 24 VDC, 1-2 A 24 VDC, 1 A Output RS232, 4-20mA, USB, Ethernet 4 RS232, RS485 Modbus, 2x 4-20mA, USB, Modbus OPC server (with BlueVIS) Maintenance once a month Maintenance yearly 1-point calibration with ambient air or nitrogen (other conditions on request) Optional factory calibration with certified gases One point calibration with ambient air once a month (other conditions on request) CE EN :1997 +A2:1998 EN :2006 / FCC 15:2009 Subpart 107/109, ICES - 001:2006 * = full scale ** = also available as cartridge for BlueVary 1 = accuracy < ±.0.5 % FS* ± 5% reading 2 = with monthly 1-point calibration 3 = not compensated 4 = Ethernet with BACCom 5 = stainless steel housing, dimensions depends on flow adapter 6 = others on request 7 = binary mixture 10
11 Everything at a glance: product overview Sensor BlueInOne Cell O2** O2ec** H2** Measuring range 0 10 Vol. % CO 2, Vol. % O Vol. % Vol. % Vol. % Vol. % CO 2, 1 50 Vol. % Vol. % O 2 Measuring principle Infrared, dual wavelengths (CO 2) / Galvanic cell (O 2) Accuracy Long-term stability 2 Lifetime sensor element Housing Aluminum, IP 65 Dimension (WxDxH) mm Dimension (WxDxH) inch Weight Housing PA6 Dimension (DxH) mm Dimension (DxH) inch Weight Material in contact with gas Connection 6 General Approx. 3 years (CO 2), approx Vol. % h operating hours at 1 bar (14.5 psi) (O 2) 170 x 150 x x 5.91 x g (8.82 lb) ZrO 2 Galvanic cell Thermal conductivity < ± 0.2 % FS* ± 3% reading < ± 2% reading / year 15,000 operating hours Approx Vol.% h operating hours at 1 bar (14.5 psi) 900 g (1.98 lb) Not available 80 x 130 Stainless steel / Viton / Sapphire / PTFE / Polymer H.L. / Nitrile ¼ 1 ¼ 6, hose connection 4-12mm etc. 900 g (1.98 lb) 80 x 130 Steel / Viton / PTFE > 3 years 900 g (1.98 lb) 80 x 130 G 1¼, GL 45, Tri-Clamp, hose connection 4-12mm etc. Operating temperature C / F Max C / F 6 Storage temperature 0 60 C / F Pressure range (absolute): bar / psi 6 absolute pressure Steel / Viton Storage humidity % RH noncondensing > 75% RH noncondensing % RH noncondensing 75% RH noncondensing Operating humidity % RH noncondensing % RH % RH noncondensing % RH 3 Integrated humidity compensation Power supply (max.) 24 VDC, 1 A 12 or 24 VDC, 1-2 A 24 VDC, 1-2 A Output Maintenance once a month Maintenance yearly CE RS232, RS485 Modbus, 2x 4-20mA, USB, Modbus OPC server (with BlueVIS) One point calibration with ambient air once a month (other conditions on request) EN :2006 / FCC 15:2009 Subpart 107/109, ICES - 001:2006 * = full scale ** = also available as cartridge for BlueVary 1 = accuracy < ±.0.5 % FS* ± 5% reading 2 = with monthly 1-point calibration 3 = not compensated 4 = Ethernet with BACCom 5 = stainless steel housing, dimensions depends on flow adapter 6 = others on request 7 = binary mixture RS232, 4-20mA, USB, Ethernet 4 1-point calibration with ambient air or nitrogen (other conditions on request) Optional factory calibration with certified gases EN :1997 +A2:
12 Blue Visit us at BlueSens.com BlueSens gas sensor GmbH Snirgelskamp Herten Germany Phone: Fax: BlueSens.com
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