RECENT ADVANCES IN DOPING ANALYSIS (2)
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1 M. DONIKE H. GEYER A. GOTZMANN U. MARECK-ENGELKE (EDITORS) RECENT ADVANCES IN DOPING ANALYSIS (2) Proceedings of the 12th Cologne Workshop on Dope Analysis 10th to 15th April 1994 SPORT und BUCH StrauB Edition Sport - Koln 1995
2 Table of Contents I Blood Analysis - Expectations - Possibilities K. I. Birkeland, P. Hemmersbach, E. Haug: Blood Sampling in Doping Control - Which New Information may be Obtained? 9 S. Rendic: Binding of Anabolic Steroids to Human Serum Albumin and Possible Interactions with other Drugs 15 S. Homing, W. SchSnzer, M. Donike: Steroid Profiling in Human and Horse Blood: Some Results 39 S. Horning, W. SchSnzer, M. Donike: GC/HR MS Determination of the Serum/Plasma Testosterone /17-a-Hydroxyprogesterone Ratio and its Significance 51 X. De la Torre, J. Segura, A. Polettini: Detection of Testosterone Esters in Human Plasma by GC/MS and GC/MS/MS 59 C. Ayotte: Determination of Free Methyltestosterone in Plasma Samples 81 II Steroid Analysis W. SchSnzer, M. Donike: Synthesis of Deuterated Steroids for GC/MS Quantification of Endogenous Steroids 93 E. Nolteernsting, H. Geyer, U. Mareck-Engelke, W. SchSnzer, M. Donike: Standardisation of the T/E-Determination by Deuterated Internal Standards 113 U. Mareck-Engelke, H. Geyer, M. Donike: Stability of Steroid Profiles (3): Ratios and Excretion Rates of Endogenous Steroids in Male Urines Collected over 24 Hours 121 U. Mareck-Engelke, H. Geyer, M. Donike: Stability of Steroid Profiles (4): Ratios and Excretion Rates of Endogenous Steroids in Female Urines Collected four Times over 24 Hours 135
3 M. Donike, U. Mareck-Engelke, S. Rauth: Statistical Evaluation of Longitudinal Studies, Part 2: The Usefulness of Subject Based Reference Ranges 157 T.J.A. Seppenwoolde-Waasdorp, R.P.G. van Heeswijk, D. de Boer, R.A.A. Maes: Evaluation of Endogenous Steroid Profiles in Urine (1) Effect of Illness and the Use of Antibiotics 167 G. Debruyckere, R. de Sagher, C. Van Peteghem: Detection of Interferences in Urinary Anabolic Steroid Analysis 173 P. Hemmersbach, S. Tomten, S. Nilsson, H. Oftebro, 0. Havrevoll, B. Oen, K. Birkeland: Illegal Use of Anabolic Agents in Animal Fattening - Consequences for Doping Analysis 185 L. D. Bowers: Effect of Sulfinpyrazone on Urinary Secretion of Steroid Conjugates 193 H. Geyer, U. Mareck-Engelke, E. Nolteernsting, G. Opfermann, M. Donike: The Matrix Problem in Calibration of the GC/MS for Endogenous Steroids. The Search for an Artificial Urine 199 W. SchSnzer, H. Geyer, A. Gotzmann, S. Horning, U. Mareck-Engelke, R. Nitschke, E. Nolteernsting, M. Donike: Endogenous Production and Excretion of Boldenone (17S-hydroxyandrosta-1,4-dien-3-one), an Androgenic Anabolic Steroid 211 HI Detection of Peptide Hormones M. Saugy, C. Cardis, L Rivier: Urinary Human Growth Hormone (U-HGH) I. Measurements in Non-Athlete Volunteers after Intra-Muscular or Subcutaneous hgh Injections 213 M. Saugy, C. Cardis, L Rivier, G. Brisson, C. Ayotte, P. Hemmersbach, E. Haug, J. Segura: Urinary Human Growth Hormone (U-HGH): II. Measurements in Competition Tests: More the Expression of the Renal Function than the Endocrinological Status 223 C. LJu, L.D. Sowers. Studies toward Confirmation of HCG using HPLC/MS 235
4 IV Improvement of Analytical Techniques K.O. BOrnsen, E. Gassmann, M. SchSr, H.M. Widmer: Matrix Assisted Laser Desorption and lonization - Mass Spectrometry: A New Analytical Tool 243 M. Herold, H. Dollekamp, R. Grimm: Micropreparative Peptide Separation by Capillary Electrophoresis 251 L Sowers, V. Abbott: Evaluation of Extraction Methods and Third Generation HPLC columns for Analysis of Diuretics 261 A. Gotzmann, W. SchSnzer, H. Geyer, M. Donike: Detection of Trenbolone by HPLC 269 D. Thieme, J. Grolie, R. Lang, R.K. Mueller: Detection of Mesocarb Metabolite by LC - TS/MS 275 F.T. Delbeke: A Threshold Level for Theophylline in Urine: Preliminary Results 285 A. Breidbach, G. Sigmund, M. Donike: Combination of Screening Procedures - Mesocarb Detection as an Example 301 D. de Boer, R.D. van Ooijen, R.A.A. Maes: Thermostable Derivatives of Mesocarb and its p-hydroxy Metabolite 305 J. Grolie, R. Lang, R.K. Mueller, D. Thieme: Detection of Tamoxifen Metabolites in Urine by GC/MS 317 D. de Boer, R.M.A. van de Wai, K.L. Bos, R.A.A. Maes: Fluoroacyl-(S)-prolyl Derivatization for the Enantiomeric Profiling of 3,4-methylenedioxymetamphetamine, Methylphenidate, Ephedrines and their Major Metabolites in Urine 329 L. Rivier, M. Saugy: Coca and Cocaine: What can be done in Doping Analysis 339 A. Tsoutsoulova-Draganova: Investigation on Products of Thiazide Diuretics in Human Urine 357 A. Lisi, R. Kazlauskas, G. Trout, E. Nolteernsting, M. Donike: Buprenorphine by GC-MS after Derivatization by Direct Extractive Alkylation 367 C. Ayotte, A. Charlebois, D. Goudreault, C Livesque: Improvements of Drug Detection and Analysis by Appropriate Preparation of the Injection Port of the Gas Chromatograph 375
5 V Organisation of Doping Analysis and Doping Control P. Hemmersbach, B. Bjerke, K. Birkeland, E. Haug: Short Report from the Doping Analysis During the XVII Olympic Winter Games 393 J. Segura, R. de la Torre, J.A. Pascual, R. Ventura, M. Farr6, R.R. Ewin, J. Cami: Antidoping Control Laboratory at the Games of the XXV Olympiad Barcelona'92. Part I. Management of Resources -403 J. Segura, R. de la Torre, J.A. Pascual, R. Ventura, M. Farre~, R.R. Ewin, J. Cami: Antidoping Control Laboratory at the Games of the XXV Olympiad Barcelona'92. Part II. Strategies and Results 413 I.Bruunshuus, D. Cowan, G. Hill, H. Olesen: Quantities and Units in Drugs of Abuse 431
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