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pipettor, or other means can be used to control the flow of acid, to slowly add the acid into a container of water until the acid has been diluted. J$&(\,-&(:;(.+&2.(6(*$:(/&$$ %+.:3/&$;+55,&,$#,::(5,+&()48 <+,&-$.&'(/$)2&,$#&$*$$)5$%# 6(-$.(;.$*((5,#1%,&'+55,&,$#8 B,)2&(&'(.(/2)&,#13*$$)(5/$)2&,$# &$&'(-,#+)=$)2:(8 Place a stopper or lid securely on the flask and invert several times to thoroughly mix. "#$%&'%()*&% %I%%,-..-/01$/%2%34.-#5%6708/%,9$:07;</%,<;//%3=>%

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11 "#$%&'%()*&% %**%%,-..-/01$/%2%34.-#5%6708/%,9$:07;</%,<;//%3=>%,<-490#5\<(+.-,.(_-)+:(.(/,/&+#&)+6*$+&ERSSV *$&&$#6+/(5FM*$&&$#6+/(5 *)$&',#1_+&&,.(M-2)))(#1&' *)$/(G&$(5+#5*)$/(G'(()(5 /'$(/8 J$&(/ N.$*(52.(_D/(% >*+)( 9#1,#((.,#1 NN9E(4(3-+*(31)$=(/3*)$&',#1F N.$*(52.(>&(;/+#5N.(*+2&,$#/ d8d/($-/&.$#1 +*,5,#+.(+*&,$#8 D/($-/&.$#1 +*,5+/+*+&+)4/&,#+.(+*&,$#8 D;&$ RSS:P $-5,)2&( +*,5 /$)2&,$# $.%+/'8 L))%$.72/,#1*$#*(#&.+&(5 +*,5:2/&6(;(.-$.:(5,#+ =(#&,)+&(5-2:('$$58 D/(+;,;(&&$.$.5,/;$/+6)( 62.(&&(&,;/&$&.+#/-(.+*,58 KN$%B.-4$740-#\<(+.LJ>H+;;.$=(5&,1'&G -,&&,#1/+-(&41$11)(/$./+-(&41)+//(/%,&' /,5(/',()5/8 P;7$%B.-4$740-#\<(+.+-+*(/',()5%'(# #$&;.$&(*&(564+)+6'$$5/+/'8 Q<-1$/\J,&.,)(3#($;.(#(3$.62&4)1)$=(/ +.(.(*$::(#5(5%'(#%$.7,#1%,&' *$#*(#&.+&(5$.`RSV/$)2&,$#/8a)$=(/ :2/&6(,#/;(*&(5;.,$.&$2/(8D/(;.$;(. 1)$=(b/$2&(./2.-+*(F&$+=$,5/7,#*$#&+*& %,&'&',/;.$52*&8K(:$=(1)$=(/+//$$# +/*$#&+:,#+&(58 D/,#1;.$*(52.(R+6$=(35,)2&(&'( +*,5+/#((5(5,#&$5(/,.(5/$)=(#&8 L55+*,5,#+-2:('$$5&$.(+*&,$# =,+;,;(&&$.$.62.(&&(8B$26)( *'(*7&'+&&'(.(+*&,$#5$(/#$& #((5&$6(*$$)(5-,./&6(-$.(+*,5G *+&+)4?(5.(+*&,$#*$::(#*(/8 C+7(/2.(&'+&/$)=(#&,/ *$:;+&,6)(%,&'&'(+*,58 N.$*((5%,&'.(+*&,$#3(#/2.,#1 &'+&+))*$:;$#(#&/+.(/+-(&$2/( %,&'&'(+*,58

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

13 Notes *$#*(#&.+&(5/$)2&,$#/&$ *$#*(#&.+&(5*$::(.*,+)1.+5( 2/()+:,#+&(-,)::+&(.,+)3 #($;.(#(3$.;$)4=,#4)*')$.,5( 1)$=(/8H-&'(+*,5+&+#4&,:( *$#&+*&/&'(1)$=(/6(,#1%$.#3 *'+#1(&'(1)$=(/,::(5,+&()48,<-490#5\<(+.-,.(_-)+:(.(/,/&+#&)+6*$+&ERSSV*$&&$# 6+/(5FM*$&&$#6+/(5 *)$&',#1_+&&,.(M-2)))(#1&';+#&/ *)$/(G'(()(5/'$(/8<(+.+*,5.(/,/&+#&+;.$#%'(#;$2.,#1 *$#*(#&.+&(5+*,5$.%'(#&'(.(,/&'(;$&(#&,+)-$./;)+/',#18 Any deviation from this SOP requires approval from PI. d8l-&(.,#/2.,#1&'+&&'(+*,5'+/ #(2&.+),?(5&'(6+/(3+#5&'+&&'(;O,/ 6(&%((#W+#5RS&'(#(2&.+),?(5 /$)2&,$#:+46(5,/;$/(5$-5$%#&'( /,#78 "#$%&'%()*&% %*&%%,-..-/01$/%2%34.-#5%6708/%,9$:07;</%,<;//%3=>%

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b/$2&(./2.-+*(F&$+=$,5/7,#*$#&+*& %,&'&',/;.$52*&8B,/;$/($-*$#&+:,#+&(5 1)$=(/+-&(.2/(8,<-490#5\P$#1;+#&/3*)$/(5G&$(5+#5 *)$/(5G'(()(5/'$(/3*$&&$#G6+/(5 *)$&',#1_+&&,.(3)+6*$+&3L#+*,5.(/,/&+#& *'(:,*+) /;)+/'+;.$# :+5($2& $- #+&2.+).266(.3 #($;.(#(3$.=,&$#8 B(&+,)(5>QN*+#6(-$2#5+&DI e(.7()(49of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e2&4)$.e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g2/&:,0,#1/2)-2.,*+*,5%,&'o U Q U /$)2&,$#/%,)) '(+&&'(/$)2&,$#&$$=(.RSS I8D/(+&+))1)+// *$#&+,#(.3+#55$#h&-,))2;=(.4',1'F/$&'( *$..$/,=(-,??5$(/#h&1(&/;,))$=(.8 L)%+4/+55+*,5/)$%)4&$&'('45.$1(# ;(.$0,5(*$:;$#(#&8B$#$&+55&'('45.$1(# ;(.$0,5(&$&'(+*,58

16 "#$%&'%()*&% %*]%%,-..-/01$/%2%34.-#5%6708/%,9$:07;</%,<;//%3=>% +*,58 a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i+)/$-$.%+/&( 5,/;$/+)8 U8 A'(:,0&2.(,/&$6(:+5(-.(/'+#5,# $-/+:(5+48A'(/$)2&,$#:2/&6(:,0(5 +#57(;&,#+-2:('$$5+#56(%()) )+6()(5+&+))&,:(//,#*(:,0,#1%,&' -2.&'(.+*,536+/($.$.1+#,*:+&&(.:+4 )(+5&$(0;)$/,$#+#5%+&(.%,))-2.&'(.,#*.(+/(&'(=,1$.$2/.(+*&,$#8 X8 A'(.(+*&,$#,/,::(5,+&(2;$#:,0,#1 %,&'=,1$.$2/6$,),#1+#5'(+& 5(=()$;:(#&8A'(.(-$.(1)+///'$2)56(

17 %'(#;$2.,#1*$#*(#&.+&(5 /2)-2.,*+*,58 2/(5+#56(/(*2.(5/&+#5,#1$.,#+/&+#5 ),7()4%,))6(*$:(&$$'$&&$'$)58 d8 L)&'$21',&,/#$&&4;,*+))4#(*(//+.4&$ '(+&&'(/$)2&,$#3,&,/Q"&$'(+&2;&$ RXS I-$.-2))*)(+#,#1;$%(.8 W8 A',/,/+/&.$#1$0,5,?,#1/$)2&,$#3/$'+=(+ :(+#/$-,::(./,#1+#5.(:$=,#1&'( /+:;)(&'+&,/:+5($-1)+//E,(+6+/7(&F$. A(-)$#E),7(&%((?(./F8 T8 L)%+4/%+/'3.,#/(+#55.4+#41)+//%+.( E$G-.$&.(+&,#1%,&';,.+#'+/$)2&,$#8 Z8 J(=(./&$.(;,.+#'+/$)2&,$#+#5#(=(. ;)+*(%+.:/$)2&,$#,#*)$/(5*$#&+,#(.8 j8 L-&(.2/(3)(+=(&'(;,.+#'+/$)2&,$#,#+ *)(+.)4)+6()(53$;(#*$#&+,#(.,#&'(-2:(G '$$5$=(.#,1'&8L%+.:/$)2&,$#,//&,)) 5(=()$;,#11+/+#5,-*$#&+,#(53:+4*+2/( (0;)$/,$#52(&$$=(.;.(//2.,?,#18Q#*( *$)53&'(/$)2&,$#*+#6(&.+#/-(..(5&$+ 1)+//6$&&)()+6()(5-$.%+/&(+#5%,&' *$#&(#&8C+7(/2.(&$)+6()*)(+.)4&'+&#$ $&'(./$)2&,$#$./$),5/'$2)56(+55(5&$ &',/6$&&)(52(&$(0;)$/,$#.,/78 B,/;$/+)\D/(+/(;+.+&(1)+//6$&&)(3 /$),5,/+55(5&$*$#&+,#(.8 J$&(/ "#$%&'%()*&% %*+%%,-..-/01$/%2%34.-#5%6708/%,9$:07;</%,<;//%3=>%

18 Procedure/Use Scale Engineering Controls/Equipment 8. Neutralization of basic solutions Up to 1000 ml of dilute acid solution (5 M or less). All work using strong acids must be performed in a properly functioning ventilated fume hood. PPE (eye, face, gloves, clothing) Eye protection: Wear tight-fitting safety goggles or safety glasses with side shields. Face protection: Wear a face shield when not protected by a lab hood sash or where there is a potential for splashes. Hand Protection: For incidental contact protection wear nitrile rubber gloves, 8 mil minimum thickness. For potential contact with larger quantities of concentrated acid, wear fluorinated rubber (e.g., Aldrich Vitoject) or neoprene, 28 mil minimum thickness. Clothing: Wear fire/flame resistant lab coat (100% cotton based); cotton based clothing/attire; full length pants or equivalent; and close-toed, closeheeled shoes. Procedure Steps and Special Precautions for this Procedure Prior to neutralizing any base solutions, ensure they have been diluted to 5 M or less (see above). When neutralizing, perform in a properly functioning fume hood and use an ice bath to control heating if volumes exceed 250 ml. Maximum acid concentration to be used for neutralizations is 5 M. Test for neutrality using ph paper or ph meter. Notes Initials of individuals using this procedure Any deviation from this SOP requires approval from PI. "#$%&'%()*&% %*I%%,-..-/01$/%2%34.-#5%6708/%,9$:07;</%,<;//%3=>%

19 "#$%#&%'%()*'&$(+,(-.%#/.$&01%##%2$34256&#%(+7'$)28)42$+(,&4)94#2%((4:;*2&9#%3$)4 &#,$($(+&%0$2<04#:,=%#,&%#-94#2%((4:294'$>$'&%&040,?,#)2$(3%:34)$(.%#/$(+.$&0&0$2 A04"#$('$9,:B(342&$+,&%#;*2&9#%3$)40$2<04#:,=%#,&%#-94#2%((4:.$&0,'%9-%>&0$26C",(), B0,34#4,),()*()4#2&,()&04'%(&4(&%>&0$26C"G H,;4 6$+(,&*#4 B)4(&$>$4# D,&4 "#$%&'%()*&% %*+%%,-..-/01$/%2%34.-#5%6708/%,9$:07;</%,<;//%3=>%

20 6FF$#80G%2%H0/4%-A%,-..-/01$/%2%34.-#5%6708/%0#%3$44<$:$#4%65.$$:$#4%,9$:07;<%I;:$JK-.:"<;%,63L% I-)#%=#%;$','$) JKKLMNJKNO I-)#%'0:%#$','$) POQPNKJNK I-)#%>:*%#$','$) POOQNLRNL H$&#$','$) PORPNLPNS "4#'0:%#$','$) POKJNRKNL 6*:>*#$','$) POOQNRLNR "#$%&'%()*&% %()%%,-..-/01$/%2%34.-#5%6708/%,9$:07;</%,<;//%3=>%