Environmental Baseline Additional Studies: 2012 Freshwater Mussel Surveys Targeting Dwarf Wedgemussel Interim Report

Transcription

1 Environmental Baseline Additional Studies: 2012 Freshwater Mussel Surveys Targeting Dwarf Wedgemussel Interim Report Triangle Expressway Southeast Extension (TIP No R-2721/R-2828/R-2829) Wake and Johnston Counties, North Carolina Prepared for: H.W. Lochner, Inc Plaza Place, Suite 202 Raleigh, NC Prepared by: 410-B Millstone Drive Hillsborough, NC July 24, 2012

2 1.0 INTRODUCTION The North Carolina Turnpike Authority (NCTA) of the North Carolina Department of Transportation (NCDOT) proposes construction of the new road corridor from NC-55 (Apex) to US-64 Bypass (Knightdale). The Dwarf Wedgemussel (Alasmidonta heterodon, DWM), which is listed by the US Fish and Wildlife Service (USFWS) as a federally endangered species, occurs in Swift Creek within the proposed action area of the project. It was first documented to occur in Swift Creek in The Swift Creek population of the DWM has been identified as essential for the recovery of the species (USFWS 1993). Since the DWM is present within the proposed action area, potential direct, indirect and cumulative impacts to this species will need to be assessed as required by Section 7 of the Endangered Species Act of 1973, as amended. In a letter to the NCTA on February 17, 2011, the USFWS indicated that an updated Environmental Baseline on the DWM population in Swift Creek would be needed to determine if the proposed action could have the potential to jeopardize the continued existence of this species. The USFWS proposed a three-tiered study to be implemented by the NCTA to develop the updated Environmental Baseline: 1. Provide an accounting (compliance/success) of conservation measures that have been implemented in Swift Creek to protect the DWM 2. Assess the effectiveness of existing conservation measures and environmental protections in Swift Creek with regard to habitat and population stability 3. Assess historic trends and current viability of the DWM population, and its habitat, in Swift Creek The Catena Group Inc. (Catena) developed a detailed study design addressing these three components. This study was implemented in March 2012, and is in various stages of completion. This Interim Report provides the data gathered from mussel surveys conducted from March to May MUSSEL SURVEY DESIGN-2012 The overall goal of this larger study is to assess the viability of DWM in Swift Creek by examining numerous attributes, such as population density, distribution within Swift Creek, and habitat suitability. It is apparent that the DWM has always been rare in Swift Creek since it was first discovered in 1991, thus population data on this species is limited. As such, one metric to gauge the viability of the DWM population is to evaluate population trends of all mussel species that occur in Swift Creek over time. With regards to the freshwater mussel fauna, Swift Creek is one of the most species rich and extensively surveyed water-bodies in North Carolina. However, nearly all of the surveys employed an informal sampling design using timed qualitative searches for mussels at various locations. The primary objective of this type of sampling is to determine presence/absence of a particular species, and is not recommended for population density studies, or long term monitoring (Strayer and Smith 2003). Thus, conclusions on population trends derived by simply analyzing the existing The Catena Group, Job Number

3 dataset without accounting for sampling variance would have inherent flaws as the dataset provides no measure of uncertainty, such as survey effort, seasonality, surveyor experience, and survey conditions (water depth, visibility, flow etc.). To account for this, a probabilitybased design that involved a number of repeat surveys at selected sites was incorporated into the 2012 mussel surveys to develop detection probabilities for the mussel species occurring in Swift Creek. These detection probabilities will assist in making inferences of trends from previous survey data. While this will not totally eliminate the unknown biases of the informally sampled dataset, it will strengthen assumptions made with regard to previous survey data being representative of the overall population. This 2012 sampling design consisted of three components, surveying specific locations where DWM was known to occur (including Little Creek, a tributary to Swift Creek), conducting timed qualitative surveys near the DWM sites, and conducting surveys in sections where prior survey data was limited (Data Gap Surveys), as detailed below. Previous DWM Locations Eleven distinct sites in Swift Creek where DWM had previously been collected were surveyed for a length of 200 feet, with the channel being segmented into three survey lanes (right, center, left). The number of each species collected and survey time was recorded for each survey lane. Five of these sites were then randomly selected, and repeat surveys were conducted on different dates. The two sites in Little Creek where DWM was found in 2003 were also surveyed in this Manner; however, they were not selected to be surveyed twice, as they had been surveyed in late 2011, and did not yield DWM. Qualitative Surveys near DWM sites Timed qualitative surveys were conducted in stream reaches of varying length near specific DWM locations. These qualitative surveys were conducted as the survey teams were moving from one DWM site to another. The survey lengths of these sites were determined in the field, with the starting and ending points recorded using GPS. The number of each mussel species collected was recorded, as were habitat conditions (substrate, depth etc.). Nine sites in Swift Creek and one site in Little Creek were surveyed in this manner Data Gap Surveys Despite the extensive surveys that have been conducted in Swift Creek, fairly large reaches existed (1-2 miles) where little or no surveys had been conducted. These gaps in survey data primarily occurred within two general reaches of Swift Creek: between Cornwallis Road and Barber Mill Road and between Barber Mill Road and Steel Bridge (Lee) Road. Timed qualitative surveys were conducted at 15 selected locations within these two general reaches, with starting and end points, species collected, and habitat data recorded. Four of these sites were randomly selected and re-surveyed on a different date. These re-surveys, as with the re-surveys of DWM sites, will factor into the generation of detection probabilities. The Catena Group, Job Number

4 The surveys were conducted by Catena personnel on the following dates in 2012: Tim Savidge: March 02, 14, 15, 29; April 04, 09; May 02 Tom Dickinson: March 14, 15; May 02 Ivy Kimbrough: March 02, 15, 29; April 04, 09, 16; May 02 Chris Sheats: March 02, 15; April 09; May 02 John Roberts: March 14, 15, 29; April 04, 16 Michael Wood: March 15; April 16; May 02 Kate Montieth: May 02 Nancy Roberts: May 02 The scientific names of all freshwater mussel species mentioned in this report follow Turgeon et al. 1998). The common names also follow this reference, with the exception of capitalization of common names. For consistency, all common names in this report are capitalized. Williams et al. (2008) provides a justification for capitalizing common names stating Capitalization helps avoid confusion by identifying standardized common names. They explain that reference to a fragile papershell could apply to a number of thin-shelled (freshwater mussel) species or to Leptodea fragilis; by capitalizing Fragile Papershell, it can easily be recognized as the common name for a distinct species (Williams et al. 2008). 3.0 RESULTS A total of 47 sites (35 in Swift Creek, nine of which were surveyed twice, and three in Little Creek) were surveyed for a total of person hours of survey time. Fifteen species were found, including six live, and two relict shells of the DWM. Two of the six DWM were gravid. The Notched Rainbow was represented by shell material only. The total number and overall catch per unit effort () of each species found is shown in Table 1, and the results for each individual site are provided in Appendix A in sequential order. Table 1. Mussel Species Found 2012 Swift Creek Surveys Alasmidonta heterodon Dwarf Wedgemussel 6 live, 2 shells 0.05/hr Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio 8, /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike 1, /hr Elliptio lanceolata Yellow Lance /hr Elliptio sp. cf. mediocris n/a /hr Elliptio sp. cf. producta Atlantic Spike /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Pyganadon cataracta Eastern Floater /hr Strophitus undulatus Creeper /hr The Catena Group, Job Number

5 Utterbackia imbecillis Paper Pondshell /hr Villosa constricta Notched Rainbow shell 0.00/hr 4.0 DISCUSSION The brief analysis summarized below is cursory at this point, as the data collected during the 2012 surveys will be added to the existing 22 year dataset for further analyses that will be part of the later reports. As discussed earlier, detection probabilities will be generated for each mussel species occurring in Swift Creek. Population and habitat trends will be analyzed in detail and provided in the future final report that covers all aspects of this larger study of Swift Creek. As with previous survey efforts, the Eastern Elliptio and Variable Spike were the two most abundant mussel species found, and the DWM was detected in low numbers. Other rare mussel species occurring in Swift Creek, the Atlantic Pigtoe, Creeper, Eastern Lampmussel, Triangle Floater and Yellow Lance, were found in relatively low numbers, as they were in previous survey efforts. Two of the 11 previous DWM sites surveyed in 2012 yielded evidence (live or shell) of DWM, where one of the sites yielded a relict shell, and the other site yielded two live DWM, with one found on the first survey, and a different individual found on the resurvey. The population viability metric of recent reproduction and recruitment can be demonstrated by observing gravid individuals (reproduction) and multiple age classes, including young individuals (recruitment). While determining the exact age of an individual mussel in the field is difficult, age can be estimated by size (total length) and growth rests. The six live DWM individuals were 25, 28, 36, 38, 38 and 45 mm long and the two relict shells were both 34 mm. Based on age determinations of 43 DWM from the upper Tar River in North Carolina by Michaelson (1995), the DWM found in Swift Creek during the 2012 surveys likely represent at least three age classes, including relatively young (3-4 year old) individuals (Table 2). Aging individuals greater than 37 mm and 6 years old is difficult, as growth rates decline as individuals age (Michaelson 1995). Table 2. Percent Composition in Age Groups (yr) adapted from Michaelson (1995) Length (mm) 1 yr 2 yr 3 yr 4 yr 5 yr 6 yr > 6 yr ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ 100 ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ 100 ~ The Catena Group, Job Number

6 N = LITERATURE CITED Michaelson, D.L. and R.J. Neves Life History and Habitat of the Endangered Dwarf Wedgemussel (Alasmidonta heterodon) (Bivalvia: Unionidae). Journal of the North American Benthological Society 14(2): Strayer, D. L., and D. R. Smith A Guide to Sampling Freshwater Mussel Populations. American Fisheries Society, Monograph 8, Bethesda, Maryland. Turgeon, D.D., J.F. Quinn, Jr., A.E. Bogan, E.V. Coan, F.G. Hochberg, W.G. Lyons, P.M. Mikkelsen, R.J. Neves, C.F.E. Roper, G. Rosenberg, B. Roth, A. Scheltema, F.G. Thompson, M. Vecchione, and J.D. Williams Common and scientific names of aquatic invertebrates from the United States and Canada: Mollusks. 2nd Edition. American Fisheries Society Special Publication 26, Bethesda, Maryland: 526 pp. U.S. Fish and Wildlife Service Dwarf Wedgemussel (Alasmidonta heterodon) Recovery Plan. Hadley, Massachusetts. 527 pp. Williams, J.D., A.E. Bogan, and J.T. Garner of Alabama and the Mobile Basin in Georgia, Mississippi and Tennessee. University of Alabama Press, Tuscaloosa. The Catena Group, Job Number

7 APPENDIX A SURVEY RESULTS BY SITE tws 4.0 p-h , TS, CS, IK Alasmidonta heterodon Dwarf Wedgemussel /hr Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr tws 3.65 p-h , TS, CS, IK Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Pyganodon cataracta Eastern Floater /hr Strophitus undulatus Creeper /hr The Catena Group, Job Number

8 tws 1.25 p-h , TS, CS, IK Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio icterina Variable Spike /hr Fusconaia masoni Atlantic Pigtoe 1 shell ~ Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr Campeloma decisum Pointed Campeloma ~ Uncommon tcg 3.9 p-h , TS, TD, JR Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Fusconaia masoni Atlantic Pigtoe /hr Pyganodon cataracta Eastern Floater /hr Strophitus undulatus Creeper /hr tcg 2.05 p-h , TS, TD, JR Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio sp. cf. producta n/a /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr The Catena Group, Job Number

9 tcg 3.5 p-h , TS, TD, JR Alasmidonta undulata Triangle Floater 2 shells ~ Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio sp. cf. producta n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Lampsilis radiata Eastern Lampmussel /hr Pyganodon cataracta Eastern Floater /hr Campeloma decisum Pointed Campeloma ~ Rare Corbicula fluminea Asian Clam ~ Very abundant tcg 2.1 p-h , TS, TD, JR Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Pyganodon cataracta Eastern Floater /hr tcg 2.75 p-h , TS, TD, JR Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Lampsilis radiata Eastern Lampmussel /hr The Catena Group, Job Number

10 Pyganodon cataracta Eastern Floater /hr Strophitus undulatus Creeper /hr ted 3.95 p-h , TD, CS, MW Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr Campeloma decisum Pointed Campeloma ~ Common ted 2.45 p-h TD, CS, MW Alasmidonta heterodon Dwarf Wedgemussel 1 shell ~ Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr Villosa constricta Notched Rainbow 2 shells ~ The Catena Group, Job Number

11 ted 3.33 p-h , TD, CS, MW Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio sp. cf. producta n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Pyganodon cataracta Eastern Floater /hr Strophitus undulatus Creeper /hr Campeloma decisum Pointed Campeloma ~ Uncommon ted 0.8 p-h , TD, CS, MW Alasmidonta heterodon Dwarf Wedgemussel /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr ted 2.17 p-h , TD, CS, MW Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Lampsilis radiata Eastern Lampmussel /hr The Catena Group, Job Number

12 ted 2.0 p-h , TD, CS, MW Elliptio complanata Eastern Elliptio /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Lampsilis radiata Eastern Lampmussel /hr tws 1.7 p-h , TS, IK, JR Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio sp. cf. producta n/a /hr Fusconaia masoni Atlantic Pigtoe 1 shell ~ Pyganodon cataracta Eastern Floater /hr Strophitus undulatus Creeper /hr Campeloma decisum Pointed Campeloma ~ Uncommon tws 2.75 p-h , TS, IK, JR Alasmidonta heterodon Dwarf Wedgemussel /hr Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio sp. cf. producta n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr The Catena Group, Job Number

13 tws 1.6 p-h , TS, IK, JR Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Fusconaia masoni Atlantic Pigtoe /hr Pyganodon cataracta Eastern Floater /hr Strophitus undulatus Creeper /hr tws 1.2 p-h , TS, IK, JR Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Lampsilis radiata Eastern Lampmussel 1 shell ~ Strophitus undulatus Creeper /hr tws 1.35 p-h , TS, IK, JR Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio roanokensis Roanoke Slabshell /hr Strophitus undulatus Creeper 1 shell ~ Elimia catenaria Gravel Elimia ~ Common The Catena Group, Job Number

14 tws 2.3 p-h , TS, IK, JR Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Campeloma decisum Pointed Campeloma ~ Common tws 2.55 p-h , TS, IK, JR Elliptio complanata Eastern Elliptio /hr Elliptio icterina Variable Spike /hr Campeloma decisum Pointed Campeloma ~ Common tws 5.0 p-h , TS, JR, IK Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Strophitus undulatus Creeper /hr Campeloma decisum Pointed Campeloma ~ Common tws 1.5 p-h , TS, IK, JR Alasmidonta heterodon Dwarf Wedgemussel /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr The Catena Group, Job Number

15 Elliptio sp. cf. mediocris n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Lampsilis radiata Eastern Lampmussel /hr Campeloma decisum Pointed Campeloma ~ Patchy Common Elimia catenaria Gravel Elimia ~ Rare tws 1.65 p-h , TS, IK, JR Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr Campeloma decisum Pointed Campeloma ~ Patchy Common tws 2.2 p-h , TS, IK, JR Alasmidonta undulata Triangle Floater 3 shells ~ Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Lampsilis radiata Eastern Lampmussel /hr Campeloma decisum Pointed Campeloma ~ Patchy Common tws 2.35 p-h , TS, IK, JR Elliptio complanata Eastern Elliptio /hr The Catena Group, Job Number

16 Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr Campeloma decisum Pointed Campeloma ~ Uncommon tws 4.15 p-h , TS, IK, JR Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr Campeloma decisum Pointed Campeloma ~ Uncommon Elimia catenaria Gravel Elimia ~ Rare tws 2.35 p-h , TS, CS, IK Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Pyganodon cataracta Eastern Floater /hr The Catena Group, Job Number

17 tws 2.2 p-h , TS, CS, IK Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio roanokensis Roanoke Slabshell /hr Lampsilis radiata Eastern Lampmussel /hr tws 4.25 p-h , TS, CS, IK Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio lanceolata Yellow Lance /hr Elliptio sp. cf. producta n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr The Catena Group, Job Number

18 tws 2.85 p-h , TS, CS, IK Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Pyganodon cataracta Eastern Floater /hr Strophitus undulatus Creeper /hr tws 3.0 p-h , TS, CS, IK Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio lanceolata Yellow Lance /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr tws 1.67 p-h , TS, CS, IK Elliptio complanata Eastern Elliptio /hr Elliptio icterina Variable Spike /hr Elliptio roanokensis Roanoke Slabshell /hr Lampsilis radiata Eastern Lampmussel /hr The Catena Group, Job Number

19 tcg 3.5 p-h , MW, JR, IK Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Fusconaia masoni Atlantic Pigtoe /hr Strophitus undulatus Creeper /hr tcg 2.5 p-h , MW, JR, IK Alasmidonta heterodon Dwarf Wedgemussel /hr Elliptio complanata Eastern Elliptio /hr Elliptio icterina Variable Spike /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr tcg 2.75 p-h , MW, JR, IK Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio icterina Variable Spike /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr The Catena Group, Job Number

20 tcg 1.75 p-h , MW, JR, IK Elliptio complanata Eastern Elliptio /hr Elliptio icterina Variable Spike /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Utterbackia imbecillis Paper Pondshell /hr tcg 1.50 p-h , MW, JR, IK Elliptio complanata Eastern Elliptio /hr Elliptio icterina Variable Spike /hr tws 4.08 p-h , TS, CS, IK Alasmidonta heterodon Dwarf Wedgemussel 1 shell ~ Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio sp. cf. producta n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr Campeloma decisum Pointed Campeloma ~ Uncommon Elimia catenaria Gravel Elimia ~ Uncommon The Catena Group, Job Number

21 tws 2.25 p-h , TS, CS, IK Alasmidonta heterodon Dwarf Wedgemussel /hr Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr Campeloma decisum Pointed Campeloma ~ Patchy Common tws 1.75 p-h , TS, CS, IK Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr Campeloma decisum Pointed Campeloma ~ Rare tws 1.77 p-h , TS, CS, IK Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Lampsilis radiata Eastern Lampmussel /hr The Catena Group, Job Number

22 Strophitus undulatus Creeper /hr Campeloma decisum Pointed Campeloma ~ Patchy Common tws 2.65 p-h , TS, CS, IK Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr Campeloma decisum Pointed Campeloma ~ Uncommon ted 3.5 p-h , TD, MW, KM, NS Elliptio complanata Eastern Elliptio /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. producta n/a /hr Lampsilis radiata Eastern Lampmussel /hr ted 3.55 p-h , TD, MW, KM, NS Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr The Catena Group, Job Number

23 Elliptio icterina Variable Spike /hr Elliptio lanceolata Yellow Lance /hr Elliptio sp. cf. mediocris n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr Strophitus undulatus Creeper /hr ted 2.18 p-h , TD, MW, KM, NS Elliptio complanata Eastern Elliptio /hr Elliptio icterina Variable Spike /hr Fusconaia masoni Atlantic Pigtoe /hr Strophitus undulatus Creeper /hr ted 3.25 p-h , TD, MW, KM, NS Alasmidonta undulata Triangle Floater /hr Elliptio complanata Eastern Elliptio /hr Elliptio congarea Carolina Slabshell /hr Elliptio icterina Variable Spike /hr Elliptio sp. cf. mediocris n/a /hr Elliptio sp. cf. producta n/a /hr Elliptio roanokensis Roanoke Slabshell /hr Fusconaia masoni Atlantic Pigtoe /hr Lampsilis radiata Eastern Lampmussel /hr The Catena Group, Job Number