Acidification Issues in Coastal Waters of the SAML Region: Responses of Organisms Louis E. Burnett Grice Marine Laboratory College of Charleston Diel cycling of hypoxia and hypercapnia Possum Point, Corsica River St Mary s College, St. Mary s River Data from MD DNR s EyesOnTheBay.net Slide borrowed from M.S. Thesis Defense of Virginia Clark Univ. of Maryland. 1
Diel cycling of hypoxia and hypercapnia Possum Point, Corsica River St Mary s College, St. Mary s River Data from MD DNR s EyesOnTheBay.net Slide borrowed from M.S. Thesis Defense of Virginia Clark Univ. of Maryland. Additional Water Quality Data National Estuarine Research Reserve System Centralized Data Management Office (CDMO) http://cdmo.baruch.sc.edu/
Field Measurements James Island Creek, SC Location Po (torr) 3 Aug 1994 1800-1900) Channel surface ( cm) bottom (107 cm) Grass surface ( cm) bottom (39 cm) 14 78 133 53 ph Sal. Temp. Pco (torr) 7.64 7.48 7.11 7.8 5 13.8 5 11 3 30.5 31.5 30.5.7. 10.8 5.3 Total Alk..67 1.86 3.06.68 Cochran & Burnett 1996 Field Measurements James Island Creek, SC Location 9% air Po saturated (torr) 3 Aug 1994 1800-1900) Channel surface ( cm) bottom (107 cm) Grass surface ( cm) bottom (39 cm) 34% air saturated 14 78 133 53 ph Sal. Temp. Pco (torr) 7.64 7.48 7.11 7.8 5 13.8 5 11 3 30.5 31.5 30.5.7. 10.8 5.3 Total Alk..67 1.86 3.06.68 3,553 µatm 14,10 µatm Cochran & Burnett 1996 3
Field Measurements James Island Creek, SC Location Po (torr) 4 Aug 1994 0630-0730) Channel surface ( cm) bottom (88 cm) Grass surface ( cm) bottom (19 cm) 51 31 54 0 ph Sal. Temp. Pco (torr) 7.08 6.48 7.11 7.06 1 1.5 3 5 8 5 5 10.7 35.6 11.6 1.7 Total Alk..58 3.07.84 3.10 Cochran & Burnett 1996 Field Measurements James Island Creek, SC Location 33% air Po saturated (torr) 4 Aug 1994 0630-0730) Channel surface ( cm) bottom (88 cm) Grass surface ( cm) bottom (19 cm) 13% air saturated 51 31 54 0 ph Sal. Temp. Pco (torr) 7.08 6.48 7.11 7.06 1 1.5 3 5 8 5 5 10.7 35.6 11.6 1.7 Total Alk..58 3.07.84 3.10 46,840 µatm 16,710 µatm Cochran & Burnett 1996 4
Oxygen and CO Data provided by Phil Dustan, CofC; Burnett 1997 Am. Zool. How do organisms respond to hypercapnia? Tissue ph depends upon strong ion difference presence of weak acids and bases CO Hyercapnia generates tissue and cellular acidification. 5
% Survival % Survival % Survival 5/9/014 http://score.dnr.sc.gov/ High CO 1.14 torr 0.15 kpa 1,500 µatm Mid CO 0.49 torr 0.066 kpa 651 µatm hard clam Mercenaria mercenaria bay scallop Argopecten irradians Eastern oyster Crassostrea virginica Ambient CO 0.5 torr 0.035 kpa 354 µatm veliger pediveliger metamorphosed Days Talmage & Gobler 009 6
% Survival % Survival % Survival % Survival % Survival % Survival 5/9/014 High CO 1.14 torr 0.15 kpa 1,500 µatm hard clam Mercenaria mercenaria bay scallop Argopecten irradians Eastern oyster Crassostrea virginica Mid CO 0.49 torr 0.066 kpa 651 µatm Ambient CO 0.5 torr 0.035 kpa 354 µatm veliger pediveliger metamorphosed Days Talmage & Gobler 009 hard clam Mercenaria mercenaria bay scallop Argopecten irradians Eastern oyster Crassostrea virginica High CO 1.14 torr 0.15 kpa 1,500 µatm Mid CO 0.49 torr 0.066 kpa 651 µatm Ambient CO 0.5 torr 0.035 kpa 354 µatm veliger pediveliger metamorphosed Days Talmage & Gobler 009 7
Intertidal Adult Oysters CO increases to 14 torr = 1 kpa = 0,000 µatm Dwyer & Burnett 1996 Allen & Burnett 008 Case Study-Blue Crab in 1% CO Cameron, J. N. 1978. J. Comp. Physiol. 13:137-141. (University of Texas at Austin, Port Aransas Marine Laboratory = Marine Science Institute) Exposed crabs to 1% CO = 7.5 torr = 1 kpa = 9,868 µatm Measured blood acid-base status with time. 8
HCO 3 - (mmol/l) HCO 3 - (mmol/l) 5/9/014 Pco (torr) 1 10 8 6 0 4 15 0.5 0 h 10 7.6 7.8 8.0 8. ph Cameron 1978 Pco (torr) 1 10 8 6 0 48 4 15 3.5 0.5 0 h 10 7.6 7.8 8.0 8. ph Cameron 1978 9
HCO 3 - (mmol/l) 5/9/014 Pco (torr) 1 10 8 6 0 48 4 15 3.5 Partial compensation! 0.5 0 h 10 7.6 7.8 8.0 8. ph Cameron 1978 Treadmill Studies Today Show, Segment 1: http://www.today.com/id/7906984 Today Show, Segment : http://www.today.com/id/6184891/vp/184 484#7909114 Other treadmill links, crabs and shrimp: http://burnettl.people.cofc.edu/research/trea dmill.php 10
Shrimp on a Treadmill Blue Crab Performance 11
Blue Crab Performance Fatigue Behaviors in Blue Crabs 0% air sat. 50% air sat. 100% air sat. Stover et al. 013 1
Fatigue Behaviors in Blue Crabs 0% air sat. 50% air sat. 50% air sat. + % CO 100% air sat. Stover et al. 013 CO -specific Effect Pco 1.5 torr P 50 (torr) large in O affinity due to CO Pco 15 torr ph Mangum & Burnett 1986 13
Chronic Hypoxia Responses Increase concentration of respiratory pigment. Increase oxygen affinity of respiratory pigment. Blue crabs do this (defur et al., 1990); response takes days. Increase concentration of hemocyanin. Increase hemocyanin oxygen affinity. Pacific Whiteleg Shrimp Litopenaeus vannamei 14
Pacific Whiteleg Shrimp Genes to make hemocyanin are turned on within 4 hours on exposure to hypoxia. HOWEVER, exposure to hypercapnia mutes this response to hypoxia (Rathburn et al., 013) References Burnett, L.E. 1997. The challenges of living in hypoxic and hypercapnic aquatic environments. Amer. Zool. 37:633-640. Cameron, J. N. 1978. Effects of hypercapnia on blood acid-base status, NaCl fluxes, and trans-gill potential in freshwater blue crabs, Callinectes sapidus. J. Comp. Physiol. 13:137-141. Cochran, R.E. and L.E. Burnett. 1996. Respiratory responses of the salt marsh animals, Fundulus heteroclitus, Leiostomus xanthurus, and Palaemonetes pugio to environmental hypoxia and hypercapnia and to the organophosphate pesticide azinphosmethyl. J. Exp. Mar. Biol. Ecol. 195:15-144. defur, P.L., C.P. Mangum, and J.E. Reese. 1990. Respiratory responses of the blue crab Callinectes sapidus to long-term hypoxia. Biol. Bull. 178:46 54. Mangum, C.P. and L.E. Burnett. 1986. The CO sensitivity of the hemocyanins and its relationship to Cl sensitivity. Biol. Bull. 171:48-63. Melzner, F. et al. 009. Physiological basis for high CO tolerance in marine ectothermic animals: preadaptation through lifestyle and ontogeny? Biogeosciences 6:313-331. Rathburn, C.K. et al., 013. Transcriptomic responses of juvenile Pacific whiteleg shrimp, Litopenaeus vannamei, to hypoxia and hypercapnia. Physiol. Genomics 45:794-807. Stover, K.K. et al., 013. Locomotory fatigue during moderate and severe hypoxia and hypercapnia in the Atlantic blue crab, Callinectes sapidus. Biol. Bull. 4:68-78. Talmage, S.C. and C.J. Gobler. 009. The effects of elevated carbon dioxide concentration on the metamorphosis, size, and survival of larval hard clams (Mercenaria mercenaria), bay scallops (Argopecten irradians), and Eastern oysters (Crassostrea virginica). Limnol. Oceanogr. 54:07-080. http://cdmo.baruch.sc.edu/ http://mddnr.chesapeakebay.net/eyesonthebay/ 15