APPENDIX B THREE RIVERS SECOND NATURE RESULTS OF RAPID INVERTEBRATE BIOLOGICAL ASSESSMENT SAMPLING PHASE 2 APRIL 2002 A-19

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APPENDIX B THREE RIVERS SECOND NATURE RESULTS OF RAPID INVERTEBRATE BIOLOGICAL ASSESSMENT SAMPLING PHASE 2 APRIL 2002 A-19

Shades Run Squaw Run Sandy Quigley Indian Powers Run Phylum Arthropoda Insecta Ephemeroptera Baetidae Baetis sp. Heptageniidae Stenonema sp. Stenonema vicarium Epeorus sp. Stenacron sp. 1 Ephemerellidae Eurylophella sp. Ephemerella sp. Caenidae Caenis sp. Plecoptera Peltoperlidae Peltoperla sp. Perlodidae Isoperla sp. 1 Chloroperlidae Alloperla sp. 1 Leuctridae Leuctra sp. Nemouridae Amphinemura sp. 1 1 1 1 Trichoptera Hydropsychidae Cheumatopsych e sp. 13 Diplectrona sp. 23 21 7 19 Hydropsyche sp. 7 1 Hydropsyche morosa 4 Philopotamidae Dolophilodes sp. Wormalidia sp. 4 Hydroptilidae Hydroptila sp. Uenoidae Neophylax sp. 49 Rhyacophilidae Rhyacophila sp. 1 Polycentropodidae Neureclipsis sp. Polycentropus sp. 1 2 1 2 A-20

Shade Run Squaw Run Sandy Quigley Indian Powers Run Phylumn Megaloptera Corydalidae Nigronia sp. Silidae Sialis sp. 1 Odonata Zygoptera Calopterygidae Calopteryx sp Anisoptera Gomphidae Ophogomphus sp. Lanthus sp. Hemiptera Gerridae P P P Veliidae P Diptera 2 Chironomidae 14 20 15 45 19 229 Simuliidae Simulium sp. 6 2 Empididae Hemerodromi a sp. 1 Chelifera sp. 1 Clinocera sp. Ceratopogonidae Tipulidae 1 Tipula sp. 1 2 6 Hexatoma sp. Antocha sp. 1 1 1 Tabanidae Chrysops sp. Tabanus sp. Psychodidae Psychoda sp. Pericoma sp. Stratiomyidae Nematelus sp. Coleoptera 1 Elmidae Dubiraphia sp. Stenelmis sp. 30 Optioservus sp. 2 A-21

RESULTS OFAPRIL 2002 RAPID INVERTEBRATE Shade Run Squaw Run Sandy Quigley Indian Crek Powers Run Phylumn Coleoptera (cont) Psephenidae Psephenus sp. 10 Ectopria sp. 1 1 Crustacea Amphipoda Gammaridae Gammarus sp. 1186 11 12 20 3 Isopoda Asellidae Caecidotea sp. 43 6 7 Decapoda Cambaridae P Orconectes obscurus 1 Cambarus Bartoni P Mollusca Bivalvia Sphaeriidae Corbiculidae Gastropoda Physidae 1 1 1 Lymnaedae Ancylidae Annelida Oligochaeta 1 4 2 1 Tubificidae Hirudinea Nematoda Turbellaria Planaria 1 Collembola 1 Acariformes Hydrachnidae Total Number Taxa 10 15 17 9 5 9 Total Number of Organisms 1323 110 71 86 30 257 Total Number of EPT Taxa* 5 5 6 2 0 4 Total Number of EPT Organism 78 26 27 8 0 23 Percent ETP Organisms 5.9 23.64 38.03 9.3 0.0 8.95 Percent A & C Organisms** 1.06 19.09 19 47 66.67 89.11 A-22

Falling Springs Deer Plum Guys Run Run (Lower) Phylum Arthropoda Insecta Ephemeroptera Baetidae Baetis sp. 1 Heptageniidae Stenonema sp. Stenonema vicarium A-23 Little Deer Blacks Run Epeorus sp. 6 10 Stenacron sp. Ephemerellidae Eurylophella sp. Ephemerella sp. Caenidae Caenis sp. 1 2 Siphlonuridae Ameletus sp. 17 Plecoptera Peltoperlidae Peltoperla sp. 1 Perlodidae Isoperla sp. 4 Chloroperlidae 2 Alloperla sp. 19 Leuctridae Leuctra sp. 86 Nemouridae Amphinemura sp. 8 409 33 70 Trichoptera Hydropsychidae Cheumatopsych e sp. 126 2 11 7 Diplectrona sp. 3 16 1 1 7 Hydropsyche sp. 3 6 2 8 Hydropsyche morosa 67 20 7 Philopotamidae Dolophilodes sp. Wormalidia sp. Hydroptilidae Hydroptila sp. Uenoidae Neophylax sp. 4 Rhyacophilidae Rhyacophila sp. Polycentropodidae Neureclipsis sp. Polycentropus sp. 3

RESULTS OF APRIL, 2002 RAPID INVERTEBRATE Falling Springs Deer Plum Guys Run Run (Lower) Little Deer Blacks Run Phylum Trichoptera (cont) Lepidostomatidae Lepidostoma sp. 2 Megaloptera Corydalidae Nigronia sp. 5 1 2 1 Silidae Sialis sp. 1 Odonata Zygoptera Calopterygidae Calopteryx sp Anisoptera Gomphidae Ophogomphus sp. Lanthus sp. Hemiptera Gerridae P Veliidae Diptera 2 1 2-2 species Chironomidae 457 113 35 20 122 8 Simuliidae Simulium sp. 5 2 25 Empididae Hemerodromi a sp. 17 2 Chelifera sp. 4 Clinocera sp. 2 Ceratopogonidae 1 Tipulidae 1 2 1 6 Tipula sp. 3 1 2 1 Tipula sp. 2 2 Hexatoma sp. 1 Antocha sp. 5 1 2 2 Tabanidae Chrysops sp. Tabanus sp. 2 Psychodidae Psychoda sp. Pericoma sp. 1 Stratiomyidae Nematelus sp. Coleoptera 1 Elmidae Dubiraphia sp. Stenelmis sp. 7 1 13 Optioservus sp. A-24

Plum Guys Run Falling Springs Run Deer (Lower) Little Deer Blacks Run Phylum GenUs Coleoptera (cont) Psephenidae Psephenus sp. 9 1 Ectopria sp. 1 Crustacea Amphipoda Gammaridae Gammarus sp. 15 21 Isopoda Asellidae Caecidotea sp. 11 2 1 Decapoda Cambaridae Orconectes obscurus Cambarus Bartoni Mollusca Bivalvia Sphaeriidae Corbiculidae Gastropoda Physidae Lymnaedae Ancylidae Annelida Oligochaeta 5 6 157 1 2 Tubificidae Hirudinea Nematoda 1 Turbellaria 1 Planaria 1 Collembola 1 5 1 Acariformes Hydrachnidae 1 Total Number Taxa 21 26 5 15 10 20 Total Number of Organisms 746 697 200 99 185 164 Total Number of EPT Taxa* 6 11 0 5 5 7 Total Number of EPT Organism 208 553 0 36 56 112 Percent EPT Organisms 27.88 79.34 0.0 36.36 30.27 68.29 Percent A & C Organisms** 61.93 17.07 96.0 21.21 65.95 6.1 A-25

Tawney Run Riddle Run Pucketa Little Pucketa Crawford Run Bailey Run Phylum Arthropoda Insecta Ephemeroptera Baetidae Baetis sp. 4 2 Heptageniidae Stenonema sp. Stenonema vicarium 3 1 Epeorus sp. 7 3 1 Stenacron sp. 4 1 Ephemerellidae Eurylophella sp. 4 1 1 3 Ephemerella sp. 8 2 Caenidae Caenis sp. 2 11 Siphlonuridae Ameletus sp. 1 Oligoneuriidae Isonychia sp. 3 Plecoptera Peltoperlidae Peltoperla sp. Perlodidae Isoperla sp. 2 11 1 1 Chloroperlidae Alloperla sp. 3 1 Leuctridae Leuctra sp. 11 2 Nemouridae Amphinemura sp. 169 1056 9 12 8 9 Trichoptera Hydropsychidae Cheumatopsych e sp. 50 28 43 8 7 16 Diplectrona sp. 55 13 2 14 2 Hydropsyche sp. 40 4 3 6 11 Hydropsyche morosa 1 11 3 Philopotamidae Dolophilodes sp. Wormalidia sp. 1 Lepoceridae Ceraclea sp. Hydroptilidae Hydroptila sp. 1 Uenoidae Neophylax sp. 5 119 1 Rhyacophilidae Rhyacophila sp. 2 7 4 Polycentropodidae Neureclipsis sp. 1 Polycentropus sp. 2 1 A-26

Shade Run Squaw Run Sandy Quigley Indian Powers Run Phylumn Megaloptera Corydalidae Nigronia sp. Silidae Sialis sp. 1 Odonata Zygoptera Calopterygidae Calopteryx sp Anisoptera Gomphidae Ophogomphus sp. Lanthus sp. Hemiptera Gerridae P P P Veliidae P Diptera 2 Chironomidae 14 20 15 45 19 229 Simuliidae Simulium sp. 6 2 Empididae Hemerodromi a sp. 1 Chelifera sp. 1 Clinocera sp. Ceratopogonidae Tipulidae 1 Tipula sp. 1 2 6 Hexatoma sp. Antocha sp. 1 1 1 Tabanidae Chrysops sp. Tabanus sp. Psychodidae Psychoda sp. Pericoma sp. Stratiomyidae Nematelus sp. Coleoptera 1 Elmidae Dubiraphia sp. Stenelmis sp. 30 Optioservus sp. 2 A-27

Tawney Run Riddle Run Pucketa Little Pucketa Crawford Run Bailey Run Phylum Coleoptera (cont) Psephenidae Psephenus sp. 28 19 12 6 1 7 Ectopria sp. 7 Crustacea Amphipoda Gammaridae Gammarus sp. 1 2 Isopoda Asellidae Caecidotea sp. 1 11 2 Decapoda Cambaridae Orconectes obscurus Cambarus Bartoni P 1 1 Mollusca Bivalvia Sphaeriidae Corbiculidae Gastropoda Physidae 4 1 Lymnaedae 1 Ancylidae Annelida Oligochaeta 4 1 3 7 7 Tubificidae Hirudinea Nematoda Turbellaria Planaria Collembola 2 Acariformes Hydrachnidae Total Number Taxa 33 21 21 15 17 22 Total Number of Organisms 577 1371 170 331 216 206 Total Number of EPT Taxa* 15 11 12 5 7 14 Total Number of EPT Organism 364 1245 85 33 43 53 Percent EPT Organisms 63.08 90.81 50.0 9.97 19.91 25.73 Percent A & C Organisms** 23.22 3.21 36.47 43.81 75.0 31.07 A-28

Phylum THREE RIVERS SECOND NATURE Little Bull Bull Chartiers Run Buffalo Arthropoda Insecta Ephemeroptera Baetidae Baetis sp. 1 Heptageniidae Stenonema sp. 32 Stenonema vicarium Epeorus sp. 1 Stenacron sp. 1 1 18 Ephemerellidae Eurylophella sp. 1 Ephemerella sp. 1 Caenidae Caenis sp. 5 9 3 100 Siphlonuridae Ameletus sp. Oligoneuriidae Isonychia sp. 1 4 Plecoptera Peltoperlidae Peltoperla sp. Perlodidae Isoperla sp. 1 Chloroperlidae 1 Alloperla sp. Leuctridae Leuctra sp. 1 Nemouridae Amphinemura sp. 4 2 1 51 Trichoptera Hydropsychidae Cheumatopsych e sp. 45 47 45 84 Diplectrona sp. 4 Hydropsyche sp. 14 8 19 Hydropsyche morosa 6 12 40 Philopotamidae Dolophilodes sp. Wormalidia sp. Hydroptilidae Hydroptila sp. 1 1 Uenoidae Neophylax sp. 1 Rhyacophilidae Rhyacophila sp. 1 10 Polycentropodidae Neureclipsis sp. Polycentropus sp. A-29

Phylum THREE RIVERS SECOND NATURE RESULTS OF APRIL, 2002 RAPID INVERTEBRATE Little Bull Bull Chartiers Run Buffalo Trichoptera (cont) Lepidostomatidae Lepidostoma sp. Philopotamidae Chimarra sp. 28 3 3 Megaloptera Corydalidae Nigronia sp. 1 2 Silidae Sialis sp. Odonata Zygoptera Calopterygidae Calopteryx sp Coenagrionidae Argia sp. 5 Anisoptera Gomphidae Ophogomphus sp. 1 Lanthus sp. Hemiptera Gerridae Veliidae Diptera 1 3 2-2 species 2 Chironomidae 49 97 67 144 Chironominae Simuliidae Simulium sp. 3 26 Empididae Hemerodromia sp. 1 5 2 Chelifera sp. Clinocera sp. Ceratopogonidae Tipulidae 1 Tipula sp. 1 4 Hexatoma sp. Antocha sp. 2 1 Tabanidae Chrysops sp. Tabanus sp. 1 Psychodidae Psychoda sp. Pericoma sp. 1 Stratiomyidae Nematelus sp. Coleoptera Elmidae Dubiraphia sp. 1 1 Stenelmis sp. 18 19 21 77 Optioservus sp. 1 6 6 A-30

Phylum THREE RIVERS SECOND NATURE Little Bull Bull Chartiers Run Buffalo Coleoptera (cont) Psephenidae Psephenus sp. 18 11 18 99 Ectopria sp. 1 Crustacea Amphipoda Gammaridae Gammarus sp. 1 3 1 14 Isopoda Asellidae Caecidotea sp. 1 4 Decapoda Cambaridae Orconectes obscurus 1 1 Cambarus Bartoni Mollusca Bivalvia Sphaeriidae Corbiculidae P Gastropoda Physidae 1 2 Lymnaedae 1 Ancylidae 1 1 Annelida Oligochaeta 9 1 Tubificidae Hirudinea Nematoda Turbellaria Planaria 1 Collembola Acariformes Hydrachnidae Total Number Taxa 19 23 21 32 Total Number of Organisms 197 243 181 753 Total Number of EPT Taxa* 8 12 6 14 Organisms 104 91 55 362 Percent EPT Organisms 52.79 37.45 30.39 48.07 Percent A & C Organisms** 24.87 39.92 41.99 19.26 * EPT = Ephemeroptera, Plecoptera, and Trichoptera (mayflies, stoneflies, and caddisflies) ** AC = Annelida and Chironomidae (segmented worms and midges) P = Present A-31

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