Flow Cytometry Run Summary

Transcription

1 Purple_ xad Page 1 of 15 Flow Cytometry Run Summary Assay: C:\Program Files\Agilent\2100 bioanalyzer\2100 expert\assays\cells\red_to_blue Series II.xsy Assay Type: Comments Acquisition Mode: User Name: Cellular This assay is for applications that apply a red reference dye and analyze blue fluorescent cells within a red population. Default Version: 2.1 Samples Run: 6 Company Instrument: Instrument Name: DE Firmware: C Serial #: DE Type: G2938C Chip Comments: Sample Name Sample Comme nt Blue Staini ng Red Sta Staini tus ng Total Event s Mock Delta Delta Delta5 plus cut Delta3 Delta5 plus cut Delta3 under 30 sec UV EGFP % of Observation Gated

2 Purple_ xad Page 2 of 15 Data Acquisition Parameters Number of Available Sample Wells (Max.) : 6 Measurement Mode : Default Graph Settings Histogram Settings : Blue : Minimum : 0.01 Maximum : Scale : log Red : Minimum : 0.01 Maximum : Scale : log Flow Cytometry Assay Details Dot Plot Settings : Blue : Minimum : 0.01 Maximum : Red : Minimum : 0.01 Maximum : 10000

3 Purple_ xad Page 3 of 15 Dot Plot Summary Mock Dot plot statistics for sample 1 : Mock Region XMean YMean #Events %Total % of gated StdDevX StdDevY CV%X CV%Y X GMean Y GMean All Events N/A Region

4 Purple_ xad Page 4 of 15 Dot Plot Summary Continued... Delta5 Dot plot statistics for sample 2 : Delta5 Region XMean YMean #Events %Total % of gated StdDevX StdDevY CV%X CV%Y X GMean Y GMean All Events N/A Region

5 Purple_ xad Page 5 of 15 Dot Plot Summary Continued... Delta3 Dot plot statistics for sample 3 : Delta3 Region XMean YMean #Events %Total % of gated StdDevX StdDevY CV%X CV%Y X GMean Y GMean All Events N/A Region

6 Purple_ xad Page 6 of 15 Dot Plot Summary Continued... Delta5 plus cut Delta3 Dot plot statistics for sample 4 : Delta5 plus cut Delta3 Region XMean YMean #Events %Total % of gated StdDevX StdDevY CV%X CV%Y X GMean Y GMean All Events N/A Region

7 Purple_ xad Page 7 of 15 Dot Plot Summary Continued... Delta5 plus cut Delta3 under 30 sec UV Dot plot statistics for sample 5 : Delta5 plus cut Delta3 under 30 sec UV Region XMean YMean #Events %Total % of gated StdDevX StdDevY CV%X CV%Y X GMean Y GMean All Events N/A Region

8 Purple_ xad Page 8 of 15 Dot Plot Summary Continued... EGFP Dot plot statistics for sample 6 : EGFP Region XMean YMean #Events %Total % of gated StdDevX StdDevY CV%X CV%Y X GMean Y GMean All Events N/A Region

9 Purple_ xad Page 9 of 15 Histogram Summary Mock Histogram statistics for sample 1 : Mock - All Events N/A Gated by Red N/A Blue on all events N/A Blue on subset Histogram statistics for sample 1 : Mock - All Events N/A Red on all events N/A

10 Purple_ xad Page 10 of 15 Histogram Summary Continued... Delta5 Histogram statistics for sample 2 : Delta5 - All Events N/A Gated by Red N/A Blue on all events N/A Blue on subset Histogram statistics for sample 2 : Delta5 - All Events N/A Red on all events N/A

11 Purple_ xad Page 11 of 15 Histogram Summary Continued... Delta3 Histogram statistics for sample 3 : Delta3 - All Events N/A Gated by Red N/A Blue on all events N/A Blue on subset Histogram statistics for sample 3 : Delta3 - All Events N/A Red on all events N/A

12 Purple_ xad Page 12 of 15 Histogram Summary Continued... Delta5 plus cut Delta3 Histogram statistics for sample 4 : Delta5 plus cut Delta3 - All Events N/A Gated by Red N/A Blue on all events N/A Blue on subset Histogram statistics for sample 4 : Delta5 plus cut Delta3 - All Events N/A Red on all events N/A

13 Purple_ xad Page 13 of 15 Histogram Summary Continued... Delta5 plus cut Delta3 under 30 sec UV Histogram statistics for sample 5 : Delta5 plus cut Delta3 under 30 sec UV - All Events N/A Gated by Red N/A Blue on all events N/A Blue on subset Histogram statistics for sample 5 : Delta5 plus cut Delta3 under 30 sec UV - All Events N/A Red on all events N/A

14 Purple_ xad Page 14 of 15 Histogram Summary Continued... EGFP Histogram statistics for sample 6 : EGFP - All Events N/A Gated by Red N/A Blue on all events N/A Blue on subset Histogram statistics for sample 6 : EGFP - All Events N/A Red on all events N/A

15 Purple_ xad Page 15 of 15 Run Logbook Description Number Source Category Sub Category Time Stamp Time Zone User Host Run ended on port 1 (Number of wells acquired: 6) Run started on port 1 (File: C:\Program Files\Agilent\21 00 bioanalyzer\210 0 expert\data\ \2100 expert_red to Blue_DE _ _ xad) Instrument Run 10/7/2010 6:23:17 PM Instrument Run 10/7/2010 5:56:57 PM Product Number : G2938C Instrument Run 10/7/2010 5:56:57 PM Name : Instrument Run 10/7/2010 5:56:57 PM Vendor : Agilent Technologies Serial# : DE Firmware : C Cartridge : Pressure Instrument Run 10/7/2010 5:56:57 PM Instrument Run 10/7/2010 5:56:57 PM Instrument Run 10/7/2010 5:56:57 PM Instrument Run 10/7/2010 5:56:57 PM