Int. Res J Pharm. App Sci., 2012; 2(6): ISSN:

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1 Research Article International Research Journal of Pharmaceutical and Applied Sciences (IRJPAS) Available online at Int. Res J Pharm. App Sci., 2012; 2(6): METHOD DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ESTIMATION OF ALFUZOSIN AND DUTASTERIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM BY RP- HPLC M. Sirisha*, A. Ravi Kumar Department of Pharmaceutical Analysis and Quality assurance, Bapatla College of pharmacy, Bapatla , Andhra Pradesh, India (Received: 18 December 2012; Accepted: 20 December, 2012; Published: 29 December, 2012) Corresponding Author s averineniravikumar@rediffmail.com Abstract: A new simple, precise, accurate and selective RP- HPLC method has been developed and validated for estimation of alfuzosin and dutasteride in pharmaceutical formulation. The detection was carried out at 216nm for both drugs. The retention time for alfu and duta were found to be 2.939min. and 7.37min. respectively. The method was validated for precision, recovery, robustness, specificity, and detection and quantification limits, in accordance with International Conference on Harmonization guidelines. Linearity was observed in the concentration range from 25% to 150% of nominal concentration of alfuzosin and dutasteride. Correlation coefficient was for both the drugs. The % recovery was found to be within the limits of the acceptance criteria with average recovery of % for alfuzosin and % for dutasteride. The % RSD below 2.0 shows the high precision of proposed method. Key words: RP-HPLC, alfuzosin and dutasteride. INTRODUCTION HPLC has gained valuable position in the field of analysis due to ease of performance, specificity, sensitivity and the analysis of sample of complex nature. This technique is commonly used for the quantitative estimation of the drug from their formulation as well as for studying their metabolites and their estimation in the biological fluids. This method offers advantages of estimating the constituents for the multi component system without prior separation and even nano quantities can be estimated. Now days, drugs are commonly used clinically and analyst is required to develop suitable method for their analysis. Alfuzosin hydrochloride (ALH) is chemically known as N-[3-[(4-amino-6, 7- dimethoxy-quinazolin-2-yl)-met- hyl amino] propyl] tetrahydrofuran-2-carboxamide with an empirical formula of C19H27N5O4 HCl and molecular weight is g/mol. ALH is alphaadrenergic blockers 1 and relaxes the muscles in the prostate and bladder neck, making it easier to urinate. It is used to improve urination in men with benign prostatic hyperplasia 2. Dutasteride (DTS), selective inhibitor of both, type 1 and type 2 isoforms of 5á-reductase (5- AR) enzyme that converts testosterone to 5ádihydrotestosterone (DHT) which is responsible for enlargement of prostate, is used in treatment of benign prostatic hyperplasia, frequently occurring in men over the age of 50 years 3. Chemically, DTS is (5á,17â)-N-{2,5 bis (trifluoromethyl)phenyl}-3- oxo-4-azaandrost-1-ene-17-carboxamide with an empirical formula C 27 H 30 F 6 N 2 O 2, representing a molecular weight of g/mol 4. Several analytical methods that have been reported for determination of ALH in biological fluids and pharma-ceutical formulation which includes Spectrophotometric and Colorimetric methods 5-6, voltammetry method 7, Direct highperformance liquid chromatographic method is also reported for the determination of enantiomers of ALH in plasma 8-9. RP-HPLC method 10-11, Liq-uid chromatography tandem mass spectrometry method 12, validated HPLC and HPTLC stabilityindicating methods and RP-HPLC method for simultaneous estimation of Alfuzosin Hydrochloride and Dutasteride MATERIALS AND METHODS Reagents and chemicals All the chemicals used of HPLC Grade collected from E. Merck, Darmstadt, Germany and milliq water was used for mobile phase preparation. Apparatus: Waters e2695alliance HPLC system connected with PDA Detector 2998 and Empower2 Software. M. Sirisha et al,

2 Commercial Formulation Alfuzosin and dutasteride Tablets are available in the market as alfusin-d in composition of alfuzosin hydrochloride (10mg), dutasteride (0.5mg). The samples were properly checked for their manufacturing license numbers, batch numbers, production, expiry dates and stored properly. Preparation and Selection of mobile phase The preliminary isocratic studies on a reverse phase C18 column with different mobile phase Combination of water and Acetonitrile were studied for Simultaneous separation of both the drugs. The optimal composition of mobile phase determined.mixed phosphate Buffer: Acetonitrile: (20:80) and filtered through 0.45μ membrane filter. Preparation of standard solution 10mg alfuzosin and 0.5mg Levosulpiride was dissolve in 50 ml of diluent (methanol) and was further diluted to get stock solution of alfuzosin and dutasteride concentration. Solution containing mixture of alfuzosin and dutasteride of six different concentrations (25%, 50%, 75%, 100% 125%, and 150% of target concentration) was prepared in the same way. Preparation of Sample Solution Sample solution containing both the drugs was prepared by dissolving tablet powder into diluent (water) ten tablets were weighed separately. Their average weights were determined. Equivalent to one tablet weight were weighed and taken in a 50 ml volumetric flask, dissolved in diluent and shaken and sonicated for about 10 minutes then filtered through 0.45μ membrane filter. The filtered Solution was further diluted in the diluent to make the final concentration Chromatographic Conditions The mobile phase, a mixture of mixed phosphate buffer and Acetonitrile (20:80) pumped at a flow rate of 1.5 ml/min through the column (Nucleosil100-5(4.6mmx125mm 5µm)) at ambient temperature.the Mobile phase was degassed prior to use under vacuum by filtration through a 0.45μ membrane filter. Both Drugs showed good absorbance at 220nm, which was selected as wavelength for further analysis. Development and validation of HPLC method Present study was conducted to obtain a new, affordable, cost-effective and convenient method for HPLC determination of alfuzosin and dutasteride in tablet dosage form. The method was validated for the Parameters like system suitability, selectivity, linearity, accuracy, precision. System Suitability System suitability study of the method was carried out by six replicate analysis of solution containing alfuzosin and dutasteride. Various chromatographic parameters such as retention time, peak area tailing factor, theoretical plates (Tangent) of the column and resolution between the peaks were determined and the method was evaluated by analyzing these parameters. Selectivity Selectivity test determines the effect of excipients on the assay result. To determine the selectivity of the method, standard sample of alfuzosin and dutasteride were injected first. Then commercial Product, blank and excipients solution were run in the instrument one after another. Linearity Linearity of the method was determined by constructing calibration curves. Standard solutions of dutasteride and alfuzosin ofdifferent concentrations level (25%, 50%, 75%, 100%, 125%, and 150%) were used for this purpose. Each measurement was carried out in six replicates and the peak Areas of the chromatograms were plotted against the concentrations to obtain the calibration curves and correlation coefficients. Accuracy (Recovery Studies) To check the degree of accuracy of the method, recovery studies were performed in triplicate by Standard addition method at 50%,100% and 150%. Known amounts of standard alfuzosin and dutasteride were added to preanalyzed samples and were subjected to the proposed HPLC method. Precision Precision was evaluated by carrying out six independent sample preparation of a single lot of formulation. The sample solution was prepared in the same manner as described in sample Preparation. Percentage relative standard deviation (%RSD) was found to be less than 2%. This proves that method is precise. RESULTS AND DISCUSSION Results of system suitability study are summarized. Six consecutive injections of the Standard solution showed uniform retention time, theoretical plate count, tailing factor and resolution For both the drugs which indicate a preise anaylsis. Chromatograms shown in figure1 and figure 2 explain that retention time for standard sample and commercial product of Alfuzosin and Dutasteride are same. This proves that, excipients have no effect on the analytical method. On the other hand, blank peak did not overlap drug peak. So the method is highly selective. A linear relationship between peak areas (average peak M. Sirisha et al,

3 areas of six replicates) versus concentrations was observed for Alfuzosin and Dutasteride in the range of 25% to 150% of nominal concentration. Correlation coefficient was for both the drugs which prove that the method is linear. Calibration curve of Alfuzosin and Dutasteride are shown in Fig 3 and 4. Name Retention Time Area 1 alfuzosin dutasteride Fig-1: Typical chromatogram of alfuzosin and dutasteride in marketed formulation. Name Retention Time Area 1 alfuzosin dutasteride Fig-2: Typical Chromatogram of standard alfuzosin and dutasteride Table 1: Result of system suitability tests of Alfuzosin and Dutasteride Alfuzosin HCl Conc(µg/ml) Area Stastical Analysis Slope: Intercept: Correlation coefficient: Dutasteride Conc(µg/ml) Area Stastical analysis Slope: Intercept: Correlation coefficient: M. Sirisha et al,

4 Method precision Six sample solutions were prepared individually using single batch of Dutasteride and Alfuzosin tablets as per the test method and injected each solution into HPLC as per methodology. Acceptance criteria The results were found to be within the acceptance criteria ; hence the method is accurate throughout the selected range. Results of accuracy study are presented in table 3. The measured value was obtained by recovery test. Spiked amount of both the drug were compared against the recovery amount. % Recovery was 99.19% for alfuzosin and % for dutasteride. All the results indicate accuracy. Linearity of Dutasteride y = 4E+06x Linearity of Alfuzosin y = 25654x R² = R² = Fig-3: Result of linearity of alfuzosin and dutasteride Table-2: Results of Method precision Method Precision Alfuzosin HCl S.No. RT Area avg stdev %RSD Dutasteride S.No. RT Area avg stdev %RSD M. Sirisha et al,

5 Table 3: Results of Recovery Studies for alfuzosin and dutasteride Alfuzosin HCl Dutasteride Accuracy standard % Avg amt.recovered %Recovery % Avg amt.recovered %Recovery CONCLUSION The new HPLC method developed and validated for simultaneous determination of alfuzosin and dutasteride pharmaceutical dosage forms and assured the satisfactory precision and accuracy and also determining lower concentration of each drug in its solid combined dosage form by RP-HPLC method. The method was found to be simple, accurate, economical and rapid and they can be applied for routine analysis in laboratories and is suitable for the quality control of the raw materials, formulations, dissolution studies and can be employed for bioequivalence studies for the same formulation. Acknowledgement: The authors are thankful to bio-leo labsb, Kukatpally, Hyderabad and Management Bapatla College of Pharmacy, Bapatla. References 1. D. Yu, et al., Research Highlights, Prostate News Pros- tate Cancer and Prostatic Diseases, 2006;9: S. A. Mc Neill, T. B. Hargreave, C. Geffriaud-Ricouard, J. Santoni and C. G. Roehrborn, Postvoid Residual Urine in Patients with Lower Urinary Tract Symptoms Sugges- tive of Benign Prostatic Hyperplasia: Pooled Analysis of Eleven Controlled Studies with Alfuzosin, Urology, 2001;57(3): Sweetman SC. editor. Martindale, The Complete Drug Reference 34th ed. London: The Pharmaceutical Press; 2005.; p Budavari, S. editor. The Merck Index, 13th ed. Whitehouse station, NJ: Merck& Co., Inc; p B. M. Ishaq, K. V. Prakash, C. H. Kumar, G. U. Rani and P. Ramakrishna, Colorimetric Determination of Alfuzosin HCl in Pharmaceutical Formulations, Journal of Pharmacy Research, 2011;4(1): B. Uslu, Voltammetric Analysis of Alfuzosin HCl in Pharmaceuticals, Human Serum and Simulated Gastric Juice, Electroanalysis, 1990; 14(12),: A. Rouchouse, M. Manoha, A. Durand and J. P. Thenot, Direct High Performance Liquid Chromatographic Determination of the Enantiomers of Alfuzosin in Plasma on a Second- Generation Α1-Acid Glycoprotein Chiral Sta-tionary Phase, Journal of Chromatography A, 1990; 506: P. Guinebault, M. Broquaire, C. Colafranceschi and J. P. Thénot, High- Performance Liquid Chromatographic Determination of Alfuzosin in Biological Fluids with Fluo- rimetric Detection and Large-Volume Injection, Journal of Separation Science, 2011; 29(15): K. S. B. Kumar, V. A. Ranjani and D. Sathyavathi, New RP-HPLC Method Development and Validation for As-say of Alfuzosin Hydrochloride in Tablet Dosage M. Sirisha et al,

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