2. Original / Resubmission: Indicate if this is the initial application or a resubmission of an application previously submitted this year.

Transcription

1 1 of 41 INSTRUCTIONS TO COMPLETE THE APPLICATION FOR A LICENCE TO FISH FOR SCIENTIFIC PURPOSES IN THE WATERS OF THE NORTHWEST TERRITORIES, YUKON NORTH SLOPE, AND NUNAVUT TERRITORY IN ACCORDANCE WITH SECTION 52 OF THE FISHERY GENERAL) REGULATIONS OF THE FISHERIES ACT) 1. Project Title: Provide a concise title reflecting the nature of the proposed work. To expedite licencing, ensure that the Project Title wording remains consistent through all applications Federal, Provincial or Territorial). 2. Original / Resubmission: Indicate if this is the initial application or a resubmission of an application previously submitted this year. 3. Date of Submission / Resubmission: Indicate the date the licence application is submitted to the DFO Office. 4. Project Information: Indicate the length of time over which the field work will be conducted. If the project has a multi-year plan, indicate if a Licence to Fish for Scientific Purposes was obtained for this project in the previous years), the previous licence number and if a postfield report was submitted. 5. Name of Organization: Provide the name of the affiliated organization of the Project Authority. 6. Project Authority: Provide the name of the person in charge of the project. 7. Address: Provide the complete address of the affiliated organization and/or Project Authority applying for the licence. 8. Vessel/Platform: If applicable, provide the name, CFV/ registration number, country of registration of vessel or platform used to conduct work activities. If no vessel will be used, leave fields empty. 9. Field Personnel: Provide the names of all individuals who will participate in the project in the field. 10. Project Objectives: Provide a brief overview of the research objectives of the project 2-3 sentences). 11. Project Description: Provide a description maximum of one page) outlining the proposed work activities, data to be recorded, samples to be retained and anticipated impacts on the environmental and fishery resources, including bycatch estimates. If this project involves tagging, include the tag type, tag series and colour Detailed SARA Impact Description: Provide a detailed description maximum of two pages), including anticipated or potential disturbances to each SARA-listed, impacts on habitats) used by the, nature of harm, and the likelihood of harm or encounters high, medium, low). If impacts are likely, specify alternatives to proposed research methodology, mitigation measures, and potential impacts on the survival or recovery of the Location: Provide the exact location of water bodies to be sampled using proper names and map coordinates. Legal names and coordinates in degrees and minutes) can be obtained from Natural Resources Canada If a water body is not listed, then a local name if and estimated coordinates must be provided. If there are several unnamed water bodies to be sampled within a small, geographic area, provide a detailed map as an attachment) showing at least three coordinates which, when connected,

2 2 of 41 enclose the proposed water bodies. If an area within a large water body is to be sampled e.g. Great Slave Lake), provide the four corner coordinates that enclose the study area or specific sampling co-ordinates. 18. Duration of Field Project: Provide the start and end date for the study for each water body. 19. Species: For each water body, provide a list of the proposed for collection and any that might be taken as incidentals. Identify any anticipated catch intentional or otherwise) of SARA. Where applicable, use both scientific and common names for each. 20. Estimated Sample Size: For each water body and, indicate the proposed sample number. If appropriate, as in the case of some invertebrates, sample size may be estimated as a weight kg). Specify number of anticipated SARA mortalities. Indicate the number / weight of fish that will be sampled and released live back into the water body and the number that will be retained and/or dead sampled. Sample size should be no larger than what is necessary for the purpose at hand. NOTE: live sample = fish caught live, briefly retained to obtain data measurements, tagging, fin clipping, scale sampling etc.) and subsequently released live at the site of capture with a reasonable expectation of survival; dead sample = fish caught live and euthanized or retrieved dead and retained for the purpose of the study. 21. Sampling: For each water body and, list the method used biopsy, fin clip etc) or type of gear, mesh sizes), and, if applicable, soak/tow times and the number of sets or tows.

3 3 of 41 APPLICATION FOR A LICENCE TO FISH FOR SCIENTIFIC PURPOSES IN THE WATERS OF THE NORTHWEST TERRITORIES, YUKON NORTH SLOPE, AND NUNAVUT TERRITORY IN ACCORDANCE WITH SECTION 52 OF THE FISHERY GENERAL) REGULATIONS OF THE FISHERIES ACT) Any person that is fishing for scientific purposes is required to obtain a Licence to Fish for Scientific Purposes. This includes activities involving fishing, or attempting to catch fish, or other activities where the potential exists for the incidental catch of fish. 1. Project Title: Gahcho Kué Project International Polar Year Project: 2. Original Licence Application: Resubmission of Application: 3. Date of Submission: dd/mm/yy) 02/18/ Project Information: Single Year Multi-Year Year 2 Date of Resubmission: dd/mm/yy) Project Information Multi-Year): Project Licenced in a previous year? Yes No Previous Licence number: S-14/ YK-A1,A2,A3 Report of Previous Field Season Submitted? Yes No 5. Duration of Field Project yyyy/mm/dd) From: 2015/05/01 To: 2015/09/30 6. Name of Organization: De Beers Canada Inc. 7. Project Authority: First Name: Veronica Last Name: Chisholm 8. Address: Number/Street/P.O. Box: City: Province/Territory/State: Country: Postal/Zip Code: Suite 300, th Street Yellowknife NT Canada X1A 1P8 Phone #: Fax #: address: Veronica.Chisholm@debeerscanada.com

4 Pg. 4 of Vessel/Platform if : Name: CFV/Registration #: Country of Registration: 10. Field Personnel: attach additional sheets) if space insufficient) First Name: Last Name: Jason Linh Corby Tatiana Paul Leah Collin Crystal Dave Kyle Andre Sarah Leanne Erik Mason Fregoe Nguyen Shurgot Le Clerc Vecsei James Arens Hyatt Hasek Artym Bachteram Lord Baker Allen Elwood Up to 7 other Golder or De Beers or University of Waterloo personnel will be identified as needed, under the supervision of the above listed personnel for all programs combined. Up to 12 Aboriginal assistants to be hired by De Beers for all programs combined. These individuals will be under the supervision of Golder or De Beers or University of Waterloo personnel.

5 Pg. 5 of 41 Project Objectives 2-3 sentences): De Beers Canada Inc. De Beers) is developing the Gahcho Kué Mine Mine) at Kennady Lake, Northwest Territories. To access the ore-bodies, the main basins of Kennady Lake Areas 2 to 7), will be dewatered, as well as a small unnamed lake Lake D1) within the watershed. Dykes will be built at the narrows between Area 7 and 8 of Kennady Lake and to divert the upper watersheds. De Beers has acquired an Authorization for Works or Undertakings Likely to Result in Serious Harm to Fish under Section 35 2)b) of the Fisheries Act for the Gahcho Kué Project from DFO Authorization No. 03-HCAA-CA ). Fish-Out Phase I of the fish-out was completed in Phase II will be necessary to remove additional fish from the areas that will be dewatered Areas 2-7 of Kennady Lake and Lake D1, Figure 1). One portion of Phase II of the fish-out of Areas 2 to 7 of Kennady Lake will be to conduct under-ice set lining and gill netting for targeted removal of Burbot and other large-bodied fish, this portion was applied for in an earlier permit request and granted in Licence # S-14/ YK on 28 January The remaining spring and summer portions of Phase II are included here in this request. The Fish-out is required prior to de-watering of the main basins of Kennady Lake as well as Lake D1. Fish Health and Fish Tissue Concurrent studies, such as the Fish health and fish tissue study are required as part of the Aquatic Effects Monitoring Program AEMP) under the Type A Water Licence MV2005L2-0015). The AEMP includes monitoring of fish health and fish tissue during the 2015 calendar year. These data will be used for comparisons with post-construction, operations, and closure fish health and tissue data. Lower Trophic Surveys The AEMP also requires monitoring of the patterns of abundance and diversity of benthic invertebrates, phytoplankton, and zooplankton in These data will be used for comparisons with post-construction, operations, and closure fish health and tissue data. Arctic Grayling Movement and Habitat Occupancy De Beers is collaborating with the University of Waterloo on an arctic grayling study that will examine timing and duration of arctic grayling spawning migrations, watershed movements, overwintering habitat, and environmental factors influencing these parameters. This study addresses the requirement to collect scientific information as part of the overall fish-out and to collect information needed to minimize impacts of mine operations on the downstream.

6 Pg. 6 of Project Description includes attachments): The Mine is located approximately 280 km northeast of Yellowknife. The diamond-bearing kimberlite deposits are vertical pipes generally located beneath Kennady Lake. The ore will be extracted by open-pitmining methods, requiring the dewatering of the main basins of Kennady Lake Areas 2 to 7), as well as a small unnamed lake Lake D1) within the watershed. Prior to construction and operation of the Mine, completion of the fish-out will be necessary to remove fish from these areas. The Project has undergone an extensive environmental impact review process though the Mackenzie Valley Environmental Review Board Panel). The Panel recommended that the Project be approved subject to the mitigation and follow-up measures outlined in their report. The Responsible Minister agreed to adopt the recommendation of the Panel subject to the implementation of the measures, developer commitments and follow-up programs contained in that report. With Project approval, De Beers submitted applications and received permits to advance construction of the Project, including Land Use Permit MV2005C0032) and a Type A Water Licence MV2005L2-0015). The 2015 sampling program will include Fish Out, Fish Health Monitoring and Fish Tissue Chemistry Surveys, Lower Trophic Surveys, and Arctic Grayling Movement and Habitat Occupancy work discussed in more detail below. Fish Out The fish-out will continue to follow DFO s General Fish-out Protocol for Lakes and Impoundments in the Northwest Territories and Nunavut; additional project-specific protocols will be developed in continued consultation with DFO. The fish-out has been discussed with DFO and with local communities over the past few years. During the spring and summer portions of Phase II of the fish-out, intensive fish sampling will be conducted to remove fish from the main basins of Kennady Lake Areas 2 to 7) as well as Lake D1. Fish will be captured by gill netting, angling, boat/backpack electrofishing, trap/fyke nets, seining and minnow trapping. All passive gear will be checked and redeployed as needed, minimum daily. After capture, all fish can be sacrificed. Fish will be sacrificed by a stunning blow to the head, followed by pithing or cervical dislocation by experienced personnel. All fish will be identified to, enumerated and weighed. For a subset of fish, length, sex and maturity may be recorded, and otoliths may be removed for age determination. Tissue samples may also be removed from a subset of fish for laboratory analysis. The particular details of sample sizes for the subsets will be determined during the development of the detailed sampling design. Fishing and processing will be conducted by fisheries technicians/biologists, assisted by local community members. To ensure that fish are not wasted, large fish in good condition will be filleted, and the fillets will be frozen, vacuum-sealed, and provided to local communities. All small fish and carcasses will be provided to local communities, incinerated on site or disposed of in the Water Management Pond in Kennady Lake.

7 Pg. 7 of Project Description continued): Fish Health Monitoring and Fish Tissue Chemistry Surveys During 2015, a fish health program will be required as part of the AEMP for Gahcho Kué. The specific objectives of this plan include the collection of fish health and fish tissue chemistry data in two core lakes Lake N11 and Area 8 of Kennady Lake) and two of three reference lakes Reference Lake 2, Reference Lake 3, East Lake) Figure 2) and to confirm sentinel selection and optimal timing of fish health collections to best quantify reproductive endpoints in the sentinel. The fish health program will involve a lethal and non-lethal field survey of small-bodied fish from two core lakes and two of three reference lakes. The survey will involve four crew members and occur in the latespring and, if necessary an early-fall program. The spring fish health survey is proposed to meet the objective of confirmation of sentinel selection and optimal timing of fish collection, while also collecting baseline fish health data for use in the future decision process. The fish health program will preferentially target one of three small-bodied fish, Lake Chub, Ninespine Stickleback or Slimy Sculpin. The program will initially target Lake Chub in the spring, but if adequate numbers cannot be captured, it will be required to return in the fall to target Ninespine Stickleback or Slimy Sculpin. All non-target fish will be released immediately unharmed. For target individuals, lethal sampling will include collection of biological information including length, weight, fish health observations, documentation of internal pathology including stomach contents, and weighing organs. Fish carcasses will be submitted for tissue chemistry, gonads will be submitted for histology and fecundity analyses, and ageing structures will be collected and archived. For non-lethal target individuals, length and weight measurements will be collected as well as recording fish health observations subsequent to being released alive. Lower Trophic Surveys The benthic invertebrate sampling program consists of collecting benthic invertebrate samples at two core AEMP lakes Lake N11 and Area 8 of Kennady Lake), two reference lakes East Lake and Reference Lake 3), two raised lakes Lake D2 and Lake D3), and five streams downstream of Kennady Lake streams K5, L2, L3, M2 and M4). Five stations will be sampled in each core lake and each reference lake. Two stations will be sampled in Lake D2 and three stations will be sampled in Lake D3, for a total of five stations combined in Lake D2 and Lake D3. One station will be sampled in each of the streams. Benthic invertebrate samples will be collected during late summer 2015 i.e., end of August to beginning of September). For benthic invertebrate lake sampling, six Ekman grab samples will be collected at each lake station using a standard Ekman grab 15 x 15 x 15 cm; with a bottom sampling area of m²). Stations will be located in water depths ranging between 4 to 8 m. Samples will be sieved individually through a 250 µm mesh screen and material retained in the mesh will be placed into a single sample container, thus creating a single composite sample consisting of six Ekman grabs for each lake station. This will result in a total of 25 lake composite samples 4 lakes x 5 stations/ lake, plus 5 samples for the D lakes). A total of 120 Ekman grabs for the core and reference lakes 4 lakes x 5 stations/lake x 6 grabs/station = 120 grabs), and 30 grabs for the D lakes 5 stations x 6 grabs/station). This will mean a total of 150 Ekman grabs collected for all lakes.

8 Pg. 8 of Project Description continued): For benthic invertebrate stream sampling in the L and M watershed, erosional stream habitat will be sampled at five stations using a Surber sampler with a bottom sampling area of m², equipped with a 250 µm mesh collecting net. Five samples will be collected at each station and samples will be preserved individually. This will result in a total of 25 stream samples 5 streams x 1 station/stream x 5 samples/station). Plankton monitoring will be conducted in two core AEMP lakes i.e., Lake N11 and Area 8), two reference lakes i.e., East Lake and Reference Lake 3), and two raised lakes i.e., Lakes D2 and D3). Five stations will be sampled in each lake of the reference and core lakes, as well as five additional stations spread across Lakes D2 and D3, for a total of 25 stations conducted by a team of two crew members. The specific objectives of the baseline plankton field program are to collect monthly plankton data i.e., three sampling events) in reference lakes and water bodies that may be impacted by the proposed Mine i.e., core lakes and raised lakes) to characterize phytoplankton and zooplankton communities throughout the open-water season and collect monthly chlorophyll a and depth-integrated nutrient data in the reference, core, and raised lakes i.e., Lakes D2 and D3) to support the monthly plankton community data. Phytoplankton samples will be collected as a depth-integrated composite sample from the euphotic zone two-times the Secchi depth). To prepare a composite sample, discrete water samples will be collected at 2-m intervals within the euphotic zone using a PVC Kemmerer water sampler. At four out of five of the sampling stations, a single phytoplankton sample will be collected. At the fifth sampling station in each lake, two phytoplankton samples will be collected and analyzed separately to provide a measure of the combined amount of variation resulting from small scale spatial variation at the station, field sampling and laboratory analysis. A total of six phytoplankton samples will be collected at each of the reference and core lakes, as well as six additional samples collected in Lakes D2 and D3 30 samples). Zooplankton samples will be collected as a single vertical haul from the full-depth of the water column using a Wildco plankton net. Zooplankton samples will be collected at the same frequency as phytoplankton 30 samples). At four out of five of the sampling stations, one depth-integrated composite water sample will be collected from the euphotic zone and filtered for chlorophyll a analysis. At the fifth sampling station, two depth-integrated composite water samples will be collected, filtered, and analyzed separately for QC purposes. A total of six chlorophyll filters will be prepared at each of the reference and core lakes, as well as six additional samples collected in Lakes D2 and D3 30 samples). A single depth-integrated nutrient sample will be collected from the euphotic zone at each sampling station, resulting in 25 samples for each parameter during each sampling event. For nutrients, one duplicate sample, one field blank and one filtering blank sample will be collected during each sampling program for QC purposes, representing 10% of nutrient samples.

9 Pg. 9 of Project Description continued): Arctic Grayling Movement and Habitat Occupancy The Arctic Grayling Thymallus arcticus) movement and habitat occupancy study, led by Dr. Heidi Swanson at the University of Waterloo, is designed to address the need to collect scientific information as part of DFO s No Net Loss Compensation program. Data will be collected on habitat use, migration and overwintering patterns in streams downstream of Kennady Lake. The information will be used to inform mitigation strategies during and after the life of mine, as well as to contribute to the scientific body of literature on these topics. This work is covered by the Animal Use Project Proposal permit held by the University of Waterloo. The movement and habitat occupancy studies will take place in both reference and affected watersheds, before and after dewatering. Telemetry will be used to track movements with a longitudinal acoustic receiver array that stretches from Kennady Lake to Kirk Lake. Fish tagging will occur in four downstream streams: K5, L1A, M4, and 410. Habitat occupancy work will be carried out in both the downstream Stream K5 to M1) and a reference watershed P1-8, and Walmsley Lake streams 1-5). The movement component of the program involves capturing fish within the affected watershed, surgically implanting them with coded acoustic transmitters Vemco V9 tags), then tracking their movement to gather information on the timing and location of annual life history stages spawning, feeding, and overwintering). A maximum of 60 Arctic Grayling will be captured and tagged. Fish will be captured at K5 stream using a fish fence installed across the narrow point of the stream wheras angling and fyke nets will be used to capture fish from the L1a, M4, and 410 streams. Tagging will be accomplished using surgical insertion, conducted by an experienced crew. The surgical procedure takes less than five minutes while the fish is under anaesthesia, after which the fish are given 5 hours to recover in an in-stream pen prior to release. To limit the exposure to stress, tagging will cease if water temperatures exceed 15ºC and no tagging will occur after July 1 st. Length, weight, sex if possible), and fish health observations will be recorded. Two scales will be collected from behind the right pectoral fin of each fish. This research is the first acoustic telemetry program conducted on adfluvial Arctic Grayling migrations in a barrenland chain-lake system. Habitat occupancy work will be conducted from July 1 st until September 24 th and will consist of randomized repeat surveys of downstream and reference stream systems. Repeat surveys are necessary to estimate false absences. From method efficacy experiments conducted in the pilot season 2014), we determined that backpack-electrofishing is the most effective way to survey for Arctic Grayling presence or absence Young of year included). When the netter captures fish, we estimate length and occasionally take a picture for ID verification, then return the fish to the stream system. By catch is expected to include Northern Pike, Lake Trout, Round Whitefish, Burbot, Lake Chub, Slimy Sculpin, and Ninespine Stickleback. When this occurs, we record the name and return the fish to the stream. Low conductivity in this area means that backpack electrofishing is non-lethal. No mortalities occurred during the 2014 pilot season. Arctic Grayling presence and absence will be correlated with 40 other environmental covariates to deduce which environmental factors contribute to stream habitat occupancy. This component of the research contributes to the rapidly expanding discipline of habitat occupancy modeling-demonstrating applicability, challenges, and innovations in an ecosystem where this method has not been applied.

10 Pg. 10 of Describe anticipated or potential disturbances to SARA-listed ; include impacts on habitats) used by the, nature of harm, and the likelihood of harm or encounters high, medium, low) add attachment if required). There are no SARA-listed documented in the water bodies proposed for sampling. 13. In accordance with Section 73 of SARA, if impacts on a listed are likely, the Project Authority should specifyadd attachment if required): a. What alternatives to the proposed method of conducting the activity have been considered? How is the chosen method the best solution to reduce impacts to the? Not applicable. b. What mitigation measures have been included, and how do they minimize the potential impacts on listed and/or habitats? What mitigation measures have been considered and not included, and for what reasons were they rejected? Not applicable. c. Will the sampling program jeopardize survival or recovery of the, in light of responses to A and B above? If not, why not? Not applicable.

11 Pg. 11 of Legal Name if : Kennady Lake 15. Local Name if : Kennady Lake N W Number or weight) Arctic Grayling large and small mesh BSM and standard gill nets up to 100 m long; 13, 19, 25, 32, 38, 51, 64, 76, 89, 102, 114, and 127 mm mesh sizes) 6-24 sets per day for up to 28 hours per set); angling; boat/backpack electrofishing; fyke/trap nets; hoop nets; minnow traps; seining Burbot As above. Lake Trout As above. Northern Pike As above. Round Whitefish As above. Lake Chub 400 Area 8 for 5100 includes 100 from As above. fish health) Area 8 for fish health) Ninespine Stickleback 400 Area 8 for 600 includes 100 from As above. fish health) Area 8 for fish health) Slimy Sculpin 400 Area 8 for 600 includes 100 from As above. fish health) Area 8 for fish health) Phytoplankton 0 ~ 1 kg PVC Kemmerer water sampler 6 integrated samples collected every 2 m of depth) Zooplankton 0 ~ 1 kg Wildco plankton net 6 single haul samples; 20 cm diameter, 80 µm mesh) Chlorophyll a 0 < 1 g 6 filtered water samples nutrient sample 0 < 1 g 5 water samples Benthic Invertebrates 0 ~ 1 kg Ekman grab 6 samples)

12 12 of Legal Name if : unknown 15. Local Name if : Lake D N W Number) Arctic Grayling large and small mesh BSM and standard gill nets up to 100 m long; 13, 19, 25, 32, 38, 51, 64, 76, 89, 102, 114, and 127 mm mesh sizes) 6-24 sets per day for up to 28 hours per set); angling; boat/backpack electrofishing; fyke/trap nets; hoop nets; minnow traps; seining Burbot As above. Lake Trout As above. Northern Pike As above. Round Whitefish As above. Lake Chub As above. Ninespine Stickleback As above. Slimy Sculpin As above.

13 13 of Legal Name if : unknown 15. Local Name if : Lake D N W Weight) Phytoplankton 0 ~ 1 kg PVC Kemmerer water sampler 6 integrated samples collected every 2 m of depth) Zooplankton 0 ~ 1 kg Wildco plankton net 6 single haul samples; 20 cm diameter, 80 µm mesh) Chlorophyll a 0 < 1 g 6 filtered water samples nutrient sample 0 < 1 g 5 water samples Benthic Invertebrates 0 ~ 1 kg Ekman grab 6 samples)

14 14 of Legal Name if : unknown 15. Local Name if : Lake D N W Weight) Phytoplankton 0 ~ 1 kg PVC Kemmerer water sampler 6 integrated samples collected every 2 m of depth) Zooplankton 0 ~ 1 kg Wildco plankton net 6 single haul samples; 20 cm diameter, 80 µm mesh) Chlorophyll a 0 < 1 g 6 filtered water samples nutrient sample 0 < 1 g 5 water samples Benthic Invertebrates 0 ~ 1 kg Ekman grab 6 samples)

15 Pg. 15 of Legal Name if : unknown 15. Local Name if : East Lake N W Number or Weight) Lake Chub small mesh BSM and standard gill nets up to 100 m long; 13, 19, 25, 32, and 38 mm mesh sizes) 6-24 sets per day for up to 28 hours per set); angling; boat/backpack electrofishing; fyke/trap nets; hoop nets; minnow traps; seining Ninespine Stickleback As above. Slimy Sculpin As above. Phytoplankton 0 ~ 1 kg PVC Kemmerer water sampler 6 integrated samples collected every 2 m of depth) Zooplankton 0 ~ 1 kg Wildco plankton net 6 single haul samples; 20 cm diameter, 80 µm mesh) Chlorophyll a 0 < 1 g 6 filtered water samples nutrient sample 0 < 1 g 5 water samples Benthic Invertebrates 0 ~ 1 kg Ekman grab 6 samples)

16 Pg. 16 of Legal Name if : unknown 15. Local Name if : Lake N N W Number or Weight) Lake Chub small mesh BSM and standard gill nets up to 100 m long; 13, 19, 25, 32, and 38 mm mesh sizes) 6-24 sets per day for up to 28 hours per set); angling; boat/backpack electrofishing; fyke/trap nets; hoop nets; minnow traps; seining Ninespine Stickleback As above. Slimy Sculpin As above. Phytoplankton 0 ~ 1 kg PVC Kemmerer water sampler 6 integrated samples collected every 2 m of depth) Zooplankton 0 ~ 1 kg Wildco plankton net 6 single haul samples; 20 cm diameter, 80 µm mesh) Chlorophyll a 0 < 1 g 6 filtered water samples nutrient sample 0 < 1 g 5 water samples Benthic Invertebrates 0 ~ 1 kg Ekman grab 6 samples)

17 Pg. 17 of Legal Name if : unknown 15. Local Name if : Reference Lake N Number) W Lake Chub small mesh BSM and standard gill nets up to 100 m long; 13, 19, 25, 32, and 38 mm mesh sizes) 6-24 sets per day for up to 28 hours per set); angling; boat/backpack electrofishing; fyke/trap nets; hoop nets; minnow traps; seining Ninespine Stickleback As above. Slimy Sculpin As above.

18 Pg. 18 of Legal Name if : unknown 15. Local Name if : Reference Lake N W Number or Weight) Lake Chub small mesh BSM and standard gill nets up to 100 m long; 13, 19, 25, 32, and 38 mm mesh sizes) 6-24 sets per day for up to 28 hours per set); angling; boat/backpack electrofishing; fyke/trap nets; hoop nets; minnow traps; seining Ninespine Stickleback As above. Slimy Sculpin As above. Phytoplankton 0 ~ 1 kg PVC Kemmerer water sampler 6 integrated samples collected every 2 m of depth) Zooplankton 0 ~ 1 kg Wildco plankton net 6 single haul samples; 20 cm diameter, 80 µm mesh) Chlorophyll a 0 < 1 g 6 filtered water samples nutrient sample 0 < 1 g 5 water samples Benthic Invertebrates 0 ~ 1 kg Ekman grab 6 samples)

19 Pg. 19 of Legal Name if : unknown 15. Local Name if : Stream K Number or Weight) Benthic Invertebrates 0 ~ 1 kg Surber Sampler 5 samples) Arctic Grayling fish fence checked every 24 hours, angling 2 rods per crew), electro-fishing, fyke net 1-6 set for 3-24 hours).

20 Pg. 20 of Legal Name if : unknown 15. Local Name if : Stream L N W Number or Weight) Benthic Invertebrates 0 ~ 1 kg Surber Sampler 5 samples) Arctic grayling Backpack Electrofishing 4 visit)

21 Pg. 21 of Legal Name if : unknown 15. Local Name if : Stream L N W Number or Weight) Benthic Invertebrates 0 ~ 1 kg Surber Sampler 5 samples) Arctic Grayling Backpack Electrofishing 4 visit), Angling 2 rods per crew)

22 Pg. 22 of Legal Name if : unknown 15. Local Name if : Stream L1b 63 28' 7.8 Number or Weight) ' 25.3 Arctic Grayling Backpack Electrofishing 4 visit)

23 Pg. 23 of Legal Name if : unknown 15. Local Name if : Stream L1a 63 28' 16.1 Number or Weight) ' 0.4 Arctic Grayling Backpack Electrofishing 4 visit), Angling 2 rods per crew), Fyke net 1-6 nets set for 3-24 hrs)

24 Pg. 24 of Legal Name if : unknown 15. Local Name if : Stream M N W Number or Weight) Benthic Invertebrates 0 ~ 1 kg Surber Sampler 5 samples) Arctic Grayling Backpack Electrofishing 4 visit), Angling 2 rods per crew)

25 Pg. 25 of Legal Name if : unknown 15. Local Name if : Stream M ' 53.7 Number or Weight) ' 39.4 Arctic Grayling Backpack Electrofishing 4 visit)

26 Pg. 26 of Legal Name if : unknown 15. Local Name if : Stream M N W Number or Weight) Benthic Invertebrates 0 ~ 1 kg Surber Sampler 5 samples) Arctic Grayling Backpack Electrofishing 4 visit)

27 Pg. 27 of Legal Name if : unknown 15. Local Name if : Stream M Number or Weight) Arctic Grayling Backpack Electrofishing 4 visit)

28 Pg. 28 of Legal Name if : unknown 15. Local Name if : Stream ' 38.1 Number or Weight) ' 17.4 Arctic Grayling Backpack Electrofishing 4 visit), Angling 2 rods per crew)

29 Pg. 29 of Legal Name if : Unknown 15. Local Name if : Stream P ' 48.2 Number or weight) ' 55.2 Arctic Grayling Backpack Electrofishing 4 visit)

30 Pg. 30 of Legal Name if : Unknown 15. Local Name if : Stream P ' 9.4 Number or weight) ' 40.4 Arctic Grayling Backpack Electrofishing 4 visit)

31 Pg. 31 of Legal Name if : Unknown 15. Local Name if : Stream P ' 22.7 Number or weight) 109 2' 58.2 Arctic Grayling Backpack Electrofishing 4 visit)

32 Pg. 32 of Legal Name if : Unknown 15. Local Name if : Stream P ' 53.2 Number or weight) 109 3' 46.5 Arctic Grayling Backpack Electrofishing 4 visit)

33 Pg. 33 of Legal Name if : Unknown 15. Local Name if : Stream P ' 18.4 Number or weight) 109 3' 36.3 Arctic Grayling Backpack Electrofishing 4 visit)

34 Pg. 34 of Legal Name if : Unknown 15. Local Name if : Stream P ' 50.7 Number or weight) 109 4' 24.8 Arctic Grayling Backpack Electrofishing 4 visit)

35 Pg. 35 of Legal Name if : Unknown 15. Local Name if : Stream P ' 25.2 Number or weight) 109 4' 48.4 Arctic Grayling Backpack Electrofishing 4 visit)

36 Pg. 36 of Legal Name if : Unknown 15. Local Name if : Stream P ' 21.8 Number or weight) 109 5' 2.1 Arctic Grayling Backpack Electrofishing 4 visit)

37 Pg. 37 of Legal Name if : Unknown 15. Local Name if : Walmsley Stream ' 10.4 Number or weight) ' 11.9 Arctic Grayling Backpack Electrofishing 4 visit)

38 Pg. 38 of Legal Name if : Unknown 15. Local Name if : Walmsley Stream ' 6.1 Number or weight) ' 38.6 Arctic Grayling Backpack Electrofishing 4 visit)

39 Pg. 39 of Legal Name if : Unknown 15. Local Name if : Walmsley Stream ' 15.6 Number or weight) ' 15.7 Arctic Grayling Backpack Electrofishing 4 visit)

40 Pg. 40 of Legal Name if : Unknown 15. Local Name if : Walmsley Stream ' 13.9 Number or weight) ' 2.2 Arctic Grayling Backpack Electrofishing 4 visit)

41 Pg. 41 of Legal Name if : Unknown 15. Local Name if : Walmsley Stream ' 4.2 Number or weight) ' 20.6 Arctic Grayling Backpack Electrofishing 4 visit)

42 N A3 L2 N16 N8 L13 D7 N18 B1 Area 2 L A Area 1 Kennady Lake Area 3 A1 A9 J1 D5 D3 N14a D2 Area 5 Area 4 Area N17 N14 D10 D1 Gahcho Kué Project I E1 E2 Area 7 Area 6 I:\CLIENTS\DE_BEERS\ \Mapping\MXD\Fish\B2014-Fish-001-GIS.mxd LEGEND Gahcho Kué Project Watercourse Waterbody Kennady Lake Watershed Boundary Kennady Lake Sub-watershed Boundary Contour 10m interval) NOTES Base data source: National Topographic Base Data NTDB) 1:50, PROJECTION: UTM Zone Kennady Lake Study Area Metres FILE No: B2014-Fish-001-GIS JOB NO: OFFICE: GOLD-CAL GAHCHO KUÉ PROJECT Scale: 1:35,000 REVISION NO: DRAWN: CW DATUM: NAD83 1 CHECK: JR DATE: November 13, 2014 Figure 1

43 Margaret Lake Stream P2 Stream M4 East Lake Stream L1a Stream L3 Stream L1b D3 D2 Stream K5 Walmsley Stream 1 Stream L2 D1 Area 8-1 Project Walmsley Stream 2 Kennady Lake i Walmsley Lake Walmsley Stream Stream Gahcho Kué P8 Stream M2 Stream M3 Stream P7 P7 Stream P8 Fletcher Lake Stream 410 N11-1 Stream P6 Stream P3 Stream P4 Stream P5 Stream P6 Stream P7 Stream P8 Stream M1 ± P6 Stream P1 Walmsley Stream 4 Reference Lake 3 Lake 3-1 Lake 410 Walmsley Stream 5 Reference Lake 2 Lake 2-1 Inset Scale: 1:400,000 Cook Lake Stream M1 M1 Stream M2 M2 Stream M3 M3 N11 M4 N Stream M4 L2 East Lake East Lake Stream L1a L1b L1a Stream L1b Stream L2 Stream L3 L3 Stream K5 D3 D3 D2 D2 D1 Kennady Lake D1 Gahcho Kué I:\CLIENTS\DE_BEERS\ \Mapping\MXD\Fish\B2015-Fish-001-GIS.mxd i Project Area GAHCHO KUÉ PROJECT LEGEND Sample Site i Gahcho Kué Project Gahcho Kué Project Area Aquatic Sampling Locations Contour 10m interval) Watercourse Winter Access Road PROJECTION: Main Map Extent 1 Waterbody Inset Extent NOTES Base data source: National Topographic Base Data NTDB) 1:50,000 DATUM: UTM Zone FILE No: OFFICE: GOLD-CAL 1 Kilometres B2015-Fish-001-GIS JOB NO: Scale:1:1,750,000 NAD83 Scale: 1:65,000 DATE: REVISION NO: DRAWN: AB February 19, CHECK: JR Figure 2