Osvaldo Jhonatan Sepulveda Villet, Ph.D.

Transcription

1 Osvaldo J. Sepulveda Villet Curriculum Vitae 1 Osvaldo Jhonatan Sepulveda Villet, Ph.D. osvaldo.sepulvedavillet@gmail.com SUMMARY OF QUALIFICATIONS 13 years of combined Molecular Ecology and genetics research experience, including mitochondrial and nuclear DNA, and RNA analyses. Proficient in theory and technical aspects of population genetics.. Experienced in theory and design of Ecology and wildlife management analyses, comprehensive knowledge of Great Lakes biotic and abiotic issues and challenges. Direct, hands-on experience in reviewing and monitoring federally-funded Great Lakes Restoration Initiative (GLRI) projects. 6 years of experience in maintaining and operating a Molecular Ecology laboratory. Proficient in training and mentoring laboratory personnel. Extensive publication record and demonstrated ability to coordinate and create collaborative research products across academic, state, and federal institutions and agencies. Experienced in theories and practices of aquaculture techniques. Designed and conducted research on nutrition, physiology, and reproductive performance in economically- and ecologically-important species. PROFESSIONAL PREPARATION Ph.D. Biology (Ecology track). University of Toledo Population genetic structure and biogeographic patterns in the yellow perch Perca flavescens: An analysis of mitochondrial and nuclear DNA markers. M.S. Biology. Central Michigan University Development of hematopoietic tissue in the penaeid shrimp Sicyonia ingentis. B.S. Aquacultural Engineering. Universidad Autonoma de Guadalajara, Mexico Influence of fatty acids derived from experimental diets on the egg quality of the prawn Macrobrachium tenellum. PROFESSIONAL EXPERIENCE USDA ARS, Milwaukee, WI USDA ARS Postdoctoral Fellow April 2012-Present Supervisor: Dr. Brian Shepherd, brian.shepherd@ars.usda.gov, (414) Producing collaborative research on yellow perch nutrition, genetic characterization of broodstocks, and probiotic enrichment of early larval feeds. Developing life-support and environmental control systems for flow-through and recirculating research aquaculture facilities. Developing experimental feeds and diet trial design in collaboration with University of Wisconsin Milwaukee faculty and USDA staff. Providing outreach to professional and advocacy groups, developing research collaborations with local and regional non-governmental stakeholders. NOAA, Silver Spring, MD January 2011 February 2012 Sea Grant Knauss Fellow, Office of Habitat Conservation Supervisor: John Iliff, john.iliff@noaa.gov, (301) Reviewed restoration project proposals from NOAA-managed Federal Funding Opportunities. Produced GIS-based documents designed to optimize project focus and selection. Collaborated with scientific staff in NOAAʼs Great Lakes Environmental laboratory, and provided expert advice on effective habitat restoration techniques, viral diseases and monitoring and evaluation of federally-funded restoration projects in the Great Lakes region. Provided guidance to Under Secretary of Commerce for Oceans and Atmosphere on future research

2 Osvaldo J. Sepulveda Villet Curriculum Vitae 2 priority areas. National Science Foundation/ University of Toledo, Toledo, OH May 2008 May 2010 NSF GK-12 STEM Fellow Supervisor: Dr. Carol Stepien, carol.stepien@utoledo.edu, (419) Co-developed science-based class activities and curriculum focused on increasing STEM awareness and enthusiasm among urban high-school students. Co-sponsored and mentored individual student research projects for local, state and regional competitions, and provided guidance on scientific principles and experimental design concepts. Achieved a two-year increase in 10 th grade Ohio Graduation Test (OGT), from a three-year average of 65% science proficiency to a two-year average of 81%, contributing to an excellent designation for the school (up from continuous improvement ). The University of Toledo, Toledo, OH January 2005 December 2010 Graduate Research Assistant Supervisor: Dr. Carol Stepien, carol.stepien@utoledo.edu, (419) Developed research on population genetics and ecology of yellow perch in the Great Lakes region and across its North American native range. Identified population genetic structure of spawning groups using mitochondrial DNA sequences and nuclear microsatellite markers. Determined lack of congruency among fishery management units and genetic structure of spawning groups in Lake Erie, and instructed fishery management staff on proper use of genetic analysis data for wildlife management decisions. Trained new students and visiting staff in molecular Ecology techniques, co-developed and updated laboratory manual, and optimized scaling of laboratory methods to increase throughput. Taught undergraduate-level environmental science curriculum in biodiversity to major- and non-major students (2 semestersʼ experience). Pfizer@Manpower, Ann Arbor, MI April 2002 December 2004 Scientist Supervisor: Jeff Brabant (no longer at company) Carried out 2-D electrophoresis-based protein analysis at large-scale, high throughput pharmaceutical research facility. Processed, catalogued, and archived tissue samples, protein extractions, 2-D gels, and analytical data. Maintained and operated protein electrophoresis and fluorescent data acquisition equipment. Maintained and ordered stock, and replenished reagent and supplies inventories for laboratory. Developed and optimized 2-D protein electrophoresis methods, trained new and visiting staff, and optimized data analysis methods on large-scale protein research projects (published in Proteomics (2008) 8: ). Central Michigan University, Mt. Pleasant, MI August 1999 April 2002 Graduate Research Assistant Supervisor: Dr. Philip Hertzler, hertz1pl@cmich.edu, (989) Developed research on embryonic development of hematopoietic tissue in crustaceans, and characterized the sequence of a novel subunit of integrin in shrimp. Maintained, ordered and replenished inventories for DNA/RNA research laboratory, and operated electrophoresis, PCR, and data acquisition equipment. Trained new students on molecular biology techniques, including messenger RNA isolation, reverse

3 Osvaldo J. Sepulveda Villet Curriculum Vitae 3 transcriptase-based DNA synthesis, PCR amplification, restriction enzyme digestion, DNA cloning (using plasmids), medium and large-scale recovery of amplified plasmids, and RFLP analysis. PUBLICATIONS Shepherd, B.S., Rees, C.B., Sepulveda-Villet, O.J., Palmquist, D.E. and Binkowski, F.P Identification of Gender in Yellow Perch by External Morphology: Validation in four Geographic Strains and Effects of Dietary Estradiol. North American Journal of Aquaculture 75: Sepulveda-Villet, O.J., and Stepien, C.A Waterscape genetics of the yellow perch (Perca flavescens): patterns across large connected ecosystems and isolated relict populations. Molecular Ecology 21: Sepulveda-Villet, O.J., and Stepien, C.A Fine-scale population genetic structure of the yellow perch Perca flavescens in Lake Erie. Canadian Journal of Fisheries and Aquatic Sciences 68: Stepien, C.A., Murphy, D.J., Lohner R.N., Haponski, A.E., and Sepulveda-Villet, O.J Status and delineation of walleye genetic stock structure across the Great Lakes. In Status of Walleye in the Great Lakes: proceedings of the 2006 symposium. Great Lakes Fishery Commission Technical Report 69, Grzybowski, M., Sepulveda-Villet, O.J., Stepien, C.A., Rosauer, D., Binkowski, F., Klaper, R., Shepherd, B., and Goetz, F Genetic variation of 17 wild populations of yellow perch from the Midwest and East coast United States using microsatellites Transactions of the American Fisheries Society. 139: Sepulveda Villet, O.J., Ford, A.M., Williams, J.D., and Stepien, C.A Population genetic diversity and phylogeographic divergence patterns of the yellow perch (Perca flavescens). Journal of Great Lakes Research. 35: Parker, A.D., Uzarski, D.G., Sepulveda-Villet, O.J., Stepien, C.A., Ruehl, C.B., and Burton, T.M Phenotypic plasticity of age-1 Great Lakes yellow perch inhabiting wetland and nearshore habitats. Transactions of the American Fisheries Society. 138: Stepien, C.A., Murphy, D.J., Lohner R.N., Sepulveda-Villet, O.J., and Haponski, A.E Signatures of vicariance, postglacial dispersal, and spawning philopatry: Population genetics of the walleye Sander vitreus. Molecular Ecology. 18: Sepulveda Villet, O.J., Ford, A.M., and Stepien, C.A A landscape genetic analysis of Great Lakes yellow perch populations in relation to their variation across North America. Ohio Journal of Science. 108: A14-A15 Bandow, J.E., Baker, J.D., Berth, M., Painter, C., Sepulveda Villet, O.J., Clark, K.A., Kilty, I., and VanBogelen, R.A Improved image analysis workflow for 2-D gels enables large-scale 2-D gelbased proteomics studies--copd biomarker discovery study. Proteomics 8: PRESENTATIONS, TRAINING EVENTS, AND COMMUNICATIONS International Association for Great Lakes Research (IAGLR) annual Conference, Ann Arbor, MI: Genetic structure of Lake Erie yellow perch populations: A comparison of two molecular tools by O.J. Sepulveda-Villet, A.M. Ford, & C.A. Stepien. Oral (Also gave this presentation at the Sigma Xi annual student research conference at the University of Toledo on ) Ohio Division of Wildlife (ODW) Annual Meeting: Genetic structure of Lake Erie yellow perch populations as discerned by microsatellite DNA by O.J. Sepulveda-Villet & C. Stepien. Poster International Association for Great Lakes Research (IAGLR), Pennsylvania State University, State College, PA: A landscape genetic analysis of yellow perch variation across North America by O.J. Sepulveda-Villet & C.A. Stepien. Oral

4 Osvaldo J. Sepulveda Villet Curriculum Vitae 4 Ohio academy of Sciences (OAS) annual meeting, Toledo, OH: A landscape genetic analysis of Great Lakes Yellow Perch populations in relation to their variation across North America by O.J. Sepulveda-Villet & C. Stepien. Oral University of Toledo Graduate Student Symposium: Genetic Stock Structure of Lake Erie Yellow Perch Spawning Groups by O.J. Sepulveda-Villet & C. Stepien. Poster American Fisheries Society (AFS) annual meeting, Ottawa, Canada: Assessing genetic diversity and divergence levels of wild yellow perch and walleye populations and applications to improving broodstock by O.J. Sepulveda- Villet & C. Stepien. Oral Sigma Xi Scientific Honorary Society: Population genetic basis for Lake Erie yellow perch stock structure and management units by O.J. Sepulveda-Villet & C. Stepien. Oral Lake Erie Fishery Genetics Managers Workshop: Population genetic basis for Lake Erie yellow perch stock structure and management units by O.J. Sepulveda- Villet & C. Stepien. Oral Midwest Fish and Wildlife Conference, Columbus, OH: Genetic history of walleye and yellow perch by A. Haponski, O.J. Sepulveda-Villet, & C. Stepien. Oral Ohio Fish and Wildlife Managers Association (OFWMA) annual meeting, Columbus, OH: Population genetic basis for Lake Erie yellow perch stock structure and management units by O.J. Sepulveda-Villet & C. Stepien. Poster Great Lakes Fishery Commission (GLFC) Lake Erie Walleye and Yellow Perch Task Groups annual meeting: Population genetic basis for Lake Erie yellow perch stock structure and management units by O.J. Sepulveda-Villet & C. Stepien. Oral GLFC Lake Erie Walleye and Yellow Perch Task Groups annual meeting: Temporal comparisons of Lake Erie yellow perch by A. Haponski, O.J. Sepulveda-Villet, & C. Stepien. Oral Lake Erie Fishery Commission Lake Erie Committee Meeting: What DNA tells us about walleye & yellow perch in Lake Erie by C. Stepien, O.J. Sepulveda-Villet, A. Haponski, & J. Banda. Oral International Association for Great Lakes Research (IAGLR) annual Conference, Toledo, OH: Genetic structure of Great Lakes yellow perch: A landscape genetic approach by O.J. Sepulveda- Villet & C. Stepien. Oral International Association for Great Lakes Research (IAGLR) annual Conference, Toledo, OH: Spatial and temporal comparisons of Lake Erie yellow perch by A. Haponski, O.J. Sepulveda-Villet, & C. Stepien. Oral Sigma Xi Scholarʼs Celebration Student Research Symposium: Toledo, OH. Genetic structure of Lake Erie yellow perch: A landscape genetic approach to test management unit congruency by O.J. Sepulveda-Villet & C. Stepien. Oral Ohio Fish Wildlife Management Association, OFWMA, Columbus, OH: Population genetic basis for Lake Erie yellow perch stock structure and Management Units by O.J. Sepulveda-Villet & C. Stepien. Poster Ohio Fish Wildlife Management Association (OFWMA) annual meeting, Columbus, OH: Spatial and temporal genetic patterns of Lake Erie yellow perch by A. Haponski, O.J. Sepulveda-Villet & C. Stepien. Poster Great Lakes Fishery Commission Lake Erie Walleye and Yellow Perch Task Groups, What DNA tells us about walleye and yellow perch in Lake Erie by C. Stepien, O.J. Sepulveda-Villet, A. Haponski, & J. Banda. Oral Lake Erie Millennium, Windsor, Canada: Population genetic basis for Lake Erie yellow perch stock structure and Management Units by O.J. Sepulveda-Villet & C. Stepien. Poster American Fisheries Society (AFS) annual conference, Pittsburgh, PA: Finescale population genetic structure of Lake Erie yellow perch Perca flavescens (Teleostei: Percidae): Any relation to management units? by O.J. Sepulveda-Villet & C. Stepien. Poster

5 Osvaldo J. Sepulveda Villet Curriculum Vitae 5 Ohio State Research Review, Columbus, OH: Fine-scale population genetic structure of Lake Erie yellow perch Perca flavescens, T. Sullivan, O.J. Sepulveda-Villet, & C. Stepien, Oral Ohio Fish Wildlife Management Association (OFWMA) annual meeting, Columbus, OH: Spatial and temporal variation of Lake Erie yellow perch (Perca flavescens) spawning groups, T. Sullivan, O.J. Sepulveda-Villet, A. Haponski, & C. Stepien. Poster International association for Great Lakes Research annual meeting, Duluth, MN: Monitoring and evaluation of coastal habitat restoration projects in the Great Lakes region, O.J. Sepulveda-Villet and T.A. Hollweg. Poster World Aquaculture Society triennial conference, Nashville, TN: Live feed enrichment with probiotics: Current methods and future perspectives on increasing finfish larviculture success, O.J. Sepulveda-Villet, B. Shepherd, F. Binkowski. Invited oral presentation AWARDS AND DISTINCTIONS 2012 USDA ARS HQ Postdoctoral Fellowship NOAA Sea Grant Knauss Fellow National Science Foundation GK-12 (DGE ) Fellow 2009 Best Oral Presentation, University of Toledo Sigma Xi Symposium 2007 International Association for Great Lakes Research (IAGLR) Scholarship International Association for Great Lakes Research Student Travel Award 2005 American Society of Ichthyologists and Herpetologists Student Travel Award 2001 World Aquaculture Society Student Travel Award 2001 College of Science and Technology Deanʼs Student Research Award, Central Michigan University 2001 College of Science and Technology Deanʼs Student Presentation Award, Central Michigan University College of Graduate Studies Student Research and Creative Endeavors Award, Central Michigan University SYNERGISTIC AND OUTREACH ACTIVITIES Aquaculture Roundtable, Wisconsin Aquaculture Association, Pewaukee, WI 2012 Site visit to USDA ARS Trout Grains Program research facility, Bozeman, MT 2012 Site visits to Bell Aquaculture (Albany and Redkey, IN) and Freshwater Farms (Urbana, OH), 2012 Toledo Area of Concern Summit, NOAA, 2011 Staff Search Committee, Lake Erie Center/ Health Sciences Campus, U. of Toledo, 2010 Information Technology and Branding Committees, IAGLR Annual Meeting, 2009 Secretary, NSF K-12 STEM Program, University of Toledo, 2009 Judge, State Science Day, The Ohio State University, 2009 Peer Reviewer: Transactions of American Fisheries Society, Journal of Great Lakes Research Faculty Search Committee, Department of Environmental Sciences, U. of Toledo, 2006 Staff Search Committee, Lake Erie Center, U. of Toledo, 2005, 2007, 2008 Mentor, NSF Research Experience for Undergraduates Program, U. of Toledo, 2007 Field Mentor, Montessori Middle School Career Week, 2006 Fassett Middle School Career Day Presenter, 2006

6 Osvaldo J. Sepulveda Villet Curriculum Vitae 6 PROFESSIONAL SKILLS Office productivity: MS Office Suite, OpenOffice.org, Google Docs, Microsoft SharePoint GIS analysis: ArcGIS suite Advanced content creation: Adobe Acrobat, Illustrator, Photoshop, Distiller, InDesign, Premiere DNA/RNA Sequencing and alignment: Bioedit, Clustal, MEGA, Sequencher, Seqscape DNA/RNA analysis: Genepop, Arlequin, Genalex, Structure, Geneclass, Barrier, Genemapper Phylogenetic analysis: Phylip, BEAST, jmodeltest, Mesquite, MrBayes Database management: Microsoft Access, Filemaker Pro Statistical Analysis: SAS, R, SPSS. Laboratory Equipment operation, management and maintenance: ABI 3130XL Bioanalyzer, GE Healthcare Ettan 2-D electrophoresis systems, GE Healthcare Typhoon 9400 scanner, PCR thermocyclers, gel-based electrophoresis, light, laser confocal, and electron microscopy.