Cell Respiration Laboratory PSI Biology
|
|
- Bethanie Wiggins
- 5 years ago
- Views:
Transcription
1 Cell Respiration Laboratory PSI Biology Name Objective Students will understand the relationship between temperature, pressure, and gas volume and will predict the effect of temperature and germination state of peas on the rate of cell respiration. Materials Each student will need an apron, gloves, and safety goggles. Each group will need the following: 50 dry peas 50 germinating peas 100 plastic (pea-sized) beads 3 Glass vials with metal washers glued on for weight 3 #3 one-hole rubber stoppers 3 1 ml graduated pipets Absorbant cotton Nonabsorbant cotton 1 plastic tray 1 water bath 1 bucket of ice 1 100ml graduated cylinder 1 thermometer Paper towels Marking pen Masking tape Stopwatch or clock with second hand Water Other materials that can be shared: Dropper bottle containing 15% KOH Sealant Time Requirements: Pre-lab prep (For teachers): See teacher notes for pre-lab procedures Lab activity: min. Procedure 1. Take the temperature of the room temperature water bath and record it in Table Label 3 pieces of paper towel as follows: 1a, 2a, 3a. These numbers will correspond to the respirometers with the same numbers. 3. Fill the graduated cylinder with 50 ml of water. 4. Place 25 germinating peas into the water in the graduated cylinder and record the total volume of peas and water inside the cylinder in Table Subtract the initial 50 ml from the final total volume. This is the volume of the germinating peas. Record this value in Table 1 for Respirometer 1a. 6. Pour out the water from the graduated cylinder and place the germinating peas onto the paper towel labeled 1a. 7. Fill the graduated cylinder with 50 ml of water again.
2 8. Place 25 dry peas into the water in the graduated cylinder. Drop plastic beads into the graduated cylinder until the total volume equals the final volume from step 4. This is to make sure that the volume of germinating peas in respirometer 1a and the volume of peas and beads in respirometer 2a are the same. 9. Pour out the water and place the dry peas and plastic beads onto paper towel 2a. 10. Fill the graduated cylinder with 50 ml of water again. 11. Add plastic beads to the water in the graduated cylinder until the total volume equals the final volume from steps 4 and Empty out the water and place the beads on the paper towel labeled 3a. 13. Assemble the respirometers. Label the glass vials 1a, 2a, and 3a. Insert the non-tapered end of one pipet into the upper surface of a rubber stopper. Put a layer of sealant around the junction between the pipet and the rubber stopper. Make sure that there is a weight attached to the vial. Repeat this to make two more respirometers. 14. Place a piece of absorbent cotton in the bottom of each of the vials. Push the cotton firmly into the bottom of the vial. 15. Saturate the cotton in the vials with a few drops of KOH. Make sure you use the same number of drops of KOH in each vial. Do not let the KOH drip onto the sides of the vial. Caution: KOH is caustic. Avoid direct skin contact. Make sure you are wearing gloves, aprons, and goggles when performing this step. 16. Place a piece of nonabsorbent cotton on top of the saturated cotton in each vial and push each piece of cotton to the bottom of each vial. 17. Add the peas, peas and beads, or beads to the appropriate respirometer by placing them on top of the cotton. 18. Place the stoppers on each vial and make sure they are on tight to prevent any leaks. 19. Place a strip of masking tape across the narrow width of the water bath about 2/3 of the way from one end and make sure the tape is anchored firmly on the outside of the pan. The tape will support the respirometers during the equilibration period. 20. Place the three respirometers into the room temperature water bath with the pipets resting o the masking tape. Start timing for a total of 7 minutes. This is the equilibration period. 21. At the end of the equilibration period, completely submerge each respirometer in the water bath. It is normal for some water to enter the pipet but if it should stop quickly. If it doesn t, you need to check for leaks. You should be able to read the volume on the pipets without moving the respirometers. 22. Once the respirometers are submerged, begin timing for 3 minutes. After the 3 minute calibration period, note the position of the water in each pipet and record this in Table 2. This is Time Take further readings at 5, 10, 15, and 20 minutes and record the values in Table Remove the respirometers from the water bath, empty them, rinse them, and dry them thoroughly as you will be reusing them. 25. Add ice to the water bath to lower the temperature to 10 0 C. Periodically check the temperature to be sure it remains constant throughout the experiment. Add ice as necessary to ensure the temperature remains at 10 0 C. 26. Repeat steps 2 through 23 with the remaining peas and beads except that the paper towels and respirometers will now be labeled 1b, 2b, and 3b. Record the data you collect from these respirometers in the appropriate places in Table Calculate the Δ Volume in the pipet at each time interval and record these values in Table 2. To do this, subtract the reading at Time 0 from the reading at each of the subsequent times for each respirometer. 28. Calculate the corrected Δ Volume by subtracting the corresponding correction factor (room temperature or 10 0 C) from the Δ Volume for each respirometer. The correction factor will be found in Table 2. You will plot these data on a graph illustrating oxygen consumption in germinating peas.
3 Table 1 Final volume (ml) Initial volume (ml) Volume of germinating peas (Final Initial, ml.) Temperature of Water Calculation of Germinating Pea Volume Respirometer 1a Respirometer 1b Table 2 Calculation of Oxygen Consumption in Germinating Pea Seeds at Room Temperature and 10 0 C Respirometer 3a: Room Temperature, Beads only (To obtain correction factor) Time interval (min): (Reading Time 0) = Room Temp. correction factor Respirometer 1a: Room Temperature, Germinating Peas (Δ Volume Room Temp. Correction factor) Respirometer 2a: Room Temperature, Dry Peas (Δ Volume Room Temp. Correction factor) Respirometer 3b: 10 0 C, Beads Only (to obtain correction factor) Time interval (min): (Reading Time 0) = 10 0 C correction factor Respirometer 1b: 10 0 C, Germinating Peas (Δ Volume 10 0 C Correction factor)
4 Respirometer 2b: 10 0 C, Dry Peas (Δ Volume 10 0 C Correction factor) Graphing Using the data from the tables, graph the rate of oxygen consumption by the germinated peas and dry peas at room temperature and at 10 0 C. Plot the dependent variable on the Y axis and the independent variable on the X axis. Label each axis and be sure to include the proper units. Don t forget to title your graph. You should have four separate lines on your graph so don t forget to label the lines appropriately or to include a legend. Analysis 1. What was the purpose of using the beads in the experiment? Why did you use the beads only readings for the correction factors? 2. In this experiment, you studied the relationship between temperature, pressure, and gas volume. You should recall that this is the ideal gas law PV = nrt where P = gas pressure, V = gas volume; n = number of gas molecules; R = gas constant; and T = gas temperature (in Kelvin). Identify each part of the ideal gas law with regards to the respirometers. Why did the water move down the pipets in your respirometers?
5 3. If temperature continues to rise, at a certain point we would expect the rate of cell respiration to decline. Why do you think this happens? 4. What do you think would happen to your rate of cell respiration if the environmental temperature dropped to 5 0 C? Why? 5. What do you think would happen to a snake s rate of cell respiration if the environmental temperature dropped to 5 0 C? Why?
6 Teacher notes: Pre-Lab Prep: Two days before the lab, place ½ of the peas to be used in a shallow pan and completely cover the peas with warm water. Cover the pan loosely to prevent the peas from drying out. Allow the peas to soak overnight. The following morning, drain the water from the peas. Saturate several pieces of paper towel with water and wring out the excess. Wrap the soaked peas in the wet paper towels and place them back into the shallow pan. Once again, loosely cover the pan. Let the peas germinate until you are ready to begin the lab activity. Check the paper towels periodically to ensure that they are not drying out. If they are, moisten them again. The day before the lab, fill one shallow pan per group with water and allow them to come to room temperature overnight.
AP Biology Lab - Cell Respiration
AP Biology Lab - Cell Respiration This investigation uses respirometry techniques to calculate the rate of oxygen consumption (cellular respiration) in germinating pea seeds. The effect of temperature
More informationAP Biology 12 Cellular Respiration Lab
AP Biology 12 Cellular Respiration Lab Background: Each individual cell is responsible for the energy exchanges necessary to sustain its ordered structure. Cells accomplish this task by breaking down nutrient
More informationLab 5- Cellular Respiration
Lab 5- Cellular Respiration Background: Many cellular processes require energy. Aerobic cellular respiration supplies energy by the oxidation of glucose. This is a complex process involving a number of
More informationC 6 H 12 O 6 + 6O 2 6H CO kilocalories of energy/mole of glucose
Objectives Before doing this lab you should understand respiration, dormancy, and germination. After doing this lab you should be able to relate gas production to respiration rate. Introduction Aerobic
More informationAPBiology Unit 2, Chapter 8
APBiology Unit 2, Chapter 8 Research Question What factors affect the rate of cellular respiration in multicellular organisms? Background Living systems require free energy and matter to maintain order,
More information1. Read the overview. What is the difference between germinating and nongerminating
Pre-lab Cell Respiration (# 5) 1. Read the overview. What is the difference between germinating and nongerminating seeds? 2. Why do seeds need oxygen? And, what would measuring the oxygen consumption of
More informationBefore doing this lab you should understand:
RVE CELL RESPIRATION OVERVIEW In this experiment you will work with seeds that are living but dormant. A seed contains an embryo plant and a food supply surrounded by a seed coat. When the necessary conditions
More informationLAB 06 Organismal Respiration
LAB 06 Organismal Respiration Objectives: To learn how a respirometer can be used to determine a respiration rate. Identify and explain the effect of seed germination on cell respiration. To design and
More informationWhat factors affect the rate of cellular respiration in multicellular organisms?
INV~t:;TIGATION 6 CELLULAR RESPIRATION* What factors affect the rate of cellular respiration in multicellular organisms? BACKGROUND Living systems require free energy and matter to maintain order, to grow,
More informationAdditional Reading General, Organic and Biological Chemistry, by Timberlake, chapter 8.
Gas Laws EXPERIMENTAL TASK Determine the mathematical relationship between the volume of a gas sample and its absolute temperature, using experimental data; and to determine the mathematical relationship
More informationBoyle s Law VC 09. Experiment 9: Gas Laws. Abstract
Experiment 9: Gas Laws VC 09 Abstract In this laboratory activity, you will experimentally confirm Boyle s Law, determine absolute zero from Gay-Lussac's Law, and determine the molecular weight of acetone,
More informationBiology Unit 2, Structure of Life, Lab Activity 2-3
Biology Unit 2, Structure of Life, Lab Activity 2-3 Cellular respiration is the release of energy from organic compounds by metabolic chemical oxidation in the mitochondria within each cell. Cellular respiration
More informationMARK SCHEME for the October/November 2013 series 9700 BIOLOGY. 9700/53 Paper 5 (Planning, Analysis and Evaluation), maximum raw mark 30
CAMBRIDGE INTERNATIONAL EXAMINATIONS GCE Advanced Subsidiary Level and GCE Advanced Level MARK SCHEME for the October/November 2013 series 9700 BIOLOGY 9700/53 Paper 5 (Planning, Analysis and Evaluation),
More informationPRESSURE-TEMPERATURE RELATIONSHIP IN GASES
PRESSURE-TEMPERATURE RELATIONSHIP IN GASES LAB PS2.PALM INTRODUCTION Gases are made up of molecules that are in constant motion and exert pressure when they collide with the walls of their container. The
More informationR: The Ideal Gas Constant Pre-Lab Assignment
R: The Ideal Gas Constant Pre-Lab Assignment Read the entire laboratory investigation and the relevant pages in your textbook, then answers the questions that follow in the space provided below. 1 Describe
More informationC6Hi (g) 6 H2O + 6 C02(g) + energy
Experient Cell Respiration 110 Cell respiration refers to the process of converting the cheical energy of organic olecules into a for iediately usable by organiss. Glucose ay be oxidized copletely if sufficient
More informationAerobic Respiration. Evaluation copy
Aerobic Respiration Computer 17 Aerobic cellular respiration is the process of converting the chemical energy of organic molecules into a form immediately usable by organisms. Glucose may be oxidized completely
More informationTarget Density Lab SCIENTIFIC. Density Inquiry Lab Activities. Introduction. Concepts. Materials. Safety Precautions. Preparation
Target Density Lab Density Inquiry Lab Activities SCIENTIFIC Introduction The concept of density is reinforced as students measure the volume and mass of an unknown liquid in a graduated cylinder, graph
More informationBOYLE S / CHARLES LAW APPARATUS - 1m long
BOYLE S / CHARLES LAW APPARATUS - 1m long Cat: MF0340-101 (combination Boyle s and Charles without mercury) DESCRIPTION: The IEC Boyle's & Charles Law apparatus is a high quality instrument designed to
More informationExploring the Properties of Gases
Exploring the Properties of Gases LabQuest 30 The purpose of this investigation is to conduct a series of experiments, each of which illustrates a different gas law. You will be given a list of equipment
More information1. Determining Solution Concentration
In this exercise you will determine the concentration of salt solutions by measuring samples with known concentration and making a calibration curve. You will review units of concentration, and how to
More informationExperiment 13 Molar Mass of a Gas. Purpose. Background. PV = nrt
Experiment 13 Molar Mass of a Gas Purpose In this experiment you will use the ideal gas law to calculate the molar mass of a volatile liquid compound by measuring the mass, volume, temperature, and pressure
More informationEnzyme Activity Lab. Wear safety goggles when handling hydrogen peroxide.
Enzyme Activity Lab This laboratory involves the use of an enzyme that will react with hydrogen peroxide. The enzyme is catalase and hydrogen peroxide (H2O2) is the substrate. The reaction is as follows:
More informationVapor Pressure of Liquids
Experiment 10 In this experiment, you will investigate the relationship between the vapor pressure of a liquid and its temperature. When a liquid is added to the Erlenmeyer flask shown in Figure 1, it
More informationIntroduction. Objectives. Hazards. Procedure
Experiment: Exploring Gases Note to Students: Check with your instructor to see which parts of this lab (Parts A, B, or C) you will complete. Introduction Gases are made up of molecules that are in constant
More informationVapor Pressure of Liquids
Vapor Pressure of Liquids In this experiment, you will investigate the relationship between the vapor pressure of a liquid and its temperature. When a liquid is added to the Erlenmeyer flask shown in Figure
More informationVapor Pressure of Liquids
Vapor Pressure of Liquids Calculator 10 In this experiment, you will investigate the relationship between the vapor pressure of a liquid and its temperature. When a liquid is added to the Erlenmeyer flask
More informationEvaluation copy. Vapor Pressure of Liquids. computer OBJECTIVES MATERIALS
Vapor Pressure of Liquids Computer 10 In this experiment, you will investigate the relationship between the vapor pressure of a liquid and its temperature. When a liquid is added to the Erlenmeyer flask
More informationVapor Pressure of Liquids
Vapor Pressure of Liquids In this experiment, you will investigate the relationship between the vapor pressure of a liquid and its temperature. When a liquid is added to the Erlenmeyer flask shown in Figure
More informationEXPERIMENT 8 Ideal Gas Law: Molecular Weight of a Vapor
EXPERIMENT 8 Ideal Gas Law: Molecular Weight of a Vapor Purpose: In this experiment you will use the ideal gas law to calculate the molecular weight of a volatile liquid compound by measuring the mass,
More informationPRE LABORATORY ASSIGNMENT: Lab Section Score: /10 READ THE LAB TEXT BEFORE ATTEMPTING THESE PROBLEMS!
EXPERIMENT # 6 Name: PRE LABORATORY ASSIGNMENT: Lab Section Score: /10 READ THE LAB TEXT BEFORE ATTEMPTING THESE PROBLEMS! 1. Calculate the height of a corresponding column of mercury (in mm) that is at
More informationExperiment #12. Gas Laws.
Goal To observe gas laws in the laboratory. Experiment #12. Gas Laws. Introduction All ideal gases, regardless of molar mass or chemical properties, follow the same gas laws under most conditions. Gas
More informationThe Decomposition of Hydrogen Peroxide
The Decomposition of Hydrogen Peroxide Calculator 12 The decomposition of hydrogen peroxide in aqueous solution proceeds very slowly. A bottle of 3% hydrogen peroxide sitting on a grocery store shelf is
More informationCHM111 Lab Gas Laws Grading Rubric
Name Team Name CHM111 Lab Gas Laws Grading Rubric Criteria Points possible Points earned Lab Performance Printed lab handout and rubric was brought to lab 3 Safety and proper waste disposal procedures
More informationMARK SCHEME for the October/November 2013 series 9700 BIOLOGY
CAMBRIDGE INTERNATIONAL EXAMINATIONS GCE Advanced Subsidiary Level and GCE Advanced Level MARK SCHEME for the October/November 2013 series 9700 BIOLOGY 9700/51 Paper 5 (Planning, Analysis and Evaluation),
More informationGeneral Chemistry I Percent Yield of Hydrogen Gas From Magnesium and HCl
Introduction For chemical reactions involving gases, gas volume measurements provide a convenient means of determining stoichiometric relationships. A gaseous product is collected in a long, thin graduated
More informationGas Laws. Figure 1: Experimental Set-up with Leveling Bulb. GCC CHM 151LL: Gas Laws GCC, 2019 page 1 of 8
Gas Laws Introduction Although we cannot see gases, we can observe their behavior and study their properties. This lab will apply several concepts from Ideal Gas Laws. You will use your knowledge of chemical
More informationExperiment 12: MOLAR VOLUME OF AN IDEAL GAS
Experiment 1: MOLAR VOLUME OF AN IDEAL GAS Purpose: Determine the molar volume of a gas at standard temperature and pressure (STP, 0 C and pressure of 1 atm) Performance Goals: Collect and measure the
More informationSalt Lowers the Freezing Point of Water
Salt Lowers the Freezing Point of Water Topic Sodium chloride (NaCl), salt, lowers the freezing point of water. Introduction Salt is added to ice in ice cream freezers because salt lowers the freezing
More informationExploring the Properties of Gases
Computer 30 The purpose of this investigation is to conduct a series of experiments, each of which illustrates a different gas law. You will be given a list of equipment and materials and some general
More informationLABORATORY TECHNIQUES. Pouring Liquids
LABORATORY TECHNIQUES Working in the chemistry laboratory you will be handling potentially dangerous substances and performing unfamiliar tasks. This section provides you with a guide to the safe laboratory
More informationDetermination of the Gas-Law Constant (R) using CO2
Determination of the Gas-Law Constant (R) using CO2 EXPERIMENT 11 Prepared by Edward L. Brown and Miranda Raines, Lee University The student will become familiar with ideal gases and how their properties
More informationVapor Pressure of Liquids
Vapor Pressure of Liquids Experiment 10 In this experiment, you will investigate the relationship between the vapor pressure of a liquid and its temperature. When a liquid is added to the Erlenmeyer flask
More informationSOLUBILITY OF A SOLID IN WATER
1516L Experiment 2 SOLUBILITY OF A SOLID IN WATER Objectives In this experiment you will determine the solubility of potassium nitrate (KNO 3 ) in water at various temperatures. You will prepare a plot
More informationThe Decomposition of Potassium Chlorate
The Decomposition of Potassium Chlorate Small quantities of molecular oxygen (O 2 ) can be obtained from the thermal decomposition of certain oxides, peroxides, and salts of oxoacids. Some examples of
More informationBefore you start sampling, be sure to read
6. DISSOLVED OXYGEN MONITORING: MONITORING: Using the Titration Method Before you start sampling, be sure to read the following pages to familiarize yourself with the equipment and the procedures that
More informationTEMPERATURE S RELATIONSHIP TO GAS & VAPOR PRESSURE
TEMPERATURE S RELATIONSHIP TO GAS & VAPOR PRESSURE Adapted from "Chemistry with Computers" Vernier Software, Portland OR, 1997 ELECTRONIC LABORATORY NOTEBOOK (ELN) INSTRUCTIONS Read the directions and
More informationPart II. Under Construction Station Instructions. Lab Station A - Blue Print: There is O 2 Here!
Lab Station A - Blue Print: There is O 2 Here! Description: In this lab, you will consider the problem: What happened to the oxygen in the air we breathed in? Air that enters the body upon inhalation contains
More informationFigure 1. Example of volume of water required for an unknown sample
Experiment Three Density Procedure Part 1.The density of a solid Obtain a solid unknown sample from your instructor. Write down the number of the unknown in your notebook. Determine the of your unknown
More informationExperimental Procedure
1 of 6 10/3/2018, 1:37 PM https://www.sciencebuddies.org/science-fair-projects/project-ideas/microbio_p009/microbiology/yeast-metabolism-aerobic-anaerobic (http://www.sciencebuddies.org /science-fair-projects/project-ideas/microbio_p009/microbiology/yeast-metabolism-aerobic-anaerobic)
More informationExploring the Properties of Gases. Evaluation copy. 10 cm in diameter and 25 cm high)
Exploring the Properties of Gases Computer 30 The purpose of this investigation is to conduct a series of experiments, each of which illustrates a different gas law. You will be given a list of equipment
More informationCHM250 Calibration and Measurement Lab. Balance Calibration
CHM250 Calibration and Measurement Lab Green Profile Balance Calibration Introduction: Balances that are properly operated, calibrated and maintained are crucial for laboratory operations. The accuracy
More informationIntermolecular Forces
Experiment 2 Intermolecular Forces Prepared by Ross S. Nord, Eastern Michigan University with large parts adapted from Chemistry with Computers by Dan D. Holmquist and Donald D. Volz PURPOSE The purpose
More informationGas Laws: Boyle s and Amonton s Laws Minneapolis Community and Technical College v.9.08
Gas Laws: Boyle s and Amonton s Laws Minneapolis Community and Technical College v.9.08 I. Introduction The purpose of this experiment is to test the extent real gases (to the limits of our measurements)
More information2. investigate the effect of solute concentration on water potential as it relates to living plant tissues.
In this lab you will: 1. investigate the processes of diffusion and osmosis in a model membrane system, and 2. investigate the effect of solute concentration on water potential as it relates to living
More informationProcedure for Operation of the Gilson Respirometer
Procedure for Operation of the Gilson Respirometer Principle of Measurements The Gilson respirometer measures gas exchange of materials in a closed system, at a constant temperature. Glass flasks or reaction
More informationAP Biology. Investigation 6: Cellular Respiration. Investigation 6: Cellular Respiration. Investigation 6: Cellular Respiration
AP Biology Learning Objectives Investigation 6: Cellular Respiration To learn how a respirometer system can be used to measure respiration rates in plant seeds or small invertebrates, such as insects or
More informationHydrostatics Physics Lab XI
Hydrostatics Physics Lab XI Objective Students will discover the basic principles of buoyancy in a fluid. Students will also quantitatively demonstrate the variance of pressure with immersion depth in
More informationGas Laws. 2 HCl(aq) + CaCO 3 (s) H 2 O(l) + CO 2 (g) + CaCl 2 (aq) HCl(aq) + NaHCO 3 (s) H 2 O(l) + CO 2 (g) + NaCl(aq)
Gas Laws Introduction: Although we cannot see gases, we can observe their behavior and study their properties. For example, we can watch a balloon filled with helium gas floating in air and conclude that
More informationLABORATORY INVESTIGATION A Study of Yeast Fermentation - Teacher Instructions
LABORATORY INVESTIGATION A Study of Yeast Fermentation - Teacher Instructions In this laboratory investigation, students measure the rate of fermentation in yeast cells. Data are collected showing that
More informationStudying Carbon Dioxide
Activity 3 Studying Carbon Dioxide GOALS In this activity you will: Generate CO 2 by various methods, then collect and characterize it. Explore how the volume of a gas varies with temperature. Compare
More informationSOLUBILITY OF A SOLID IN WATER
1516L Experiment 1 SOLUBILITY OF A SOLID IN WATER Objectives In this experiment you will determine the solubility of potassium nitrate (KNO 3 ) in water at various temperatures. You will prepare a plot
More informationBASIC LABORATORY TECHNIQUES (Revised )
BASIC LABORATORY TECHNIQUES (Revised 1-2-16) (See Appendix II: Summary for making Spreadsheets and Graphs with Excel and Appendix III parts C, C1 and C2: Significant figures, scientific notation and rounding)
More informationGas Laws. 2 HCl(aq) + CaCO 3 (s) H 2 O(l) + CO 2 (g) + CaCl 2 (aq) HCl(aq) + NaHCO 3 (s) H 2 O(l) + CO 2 (g) + NaCl(aq)
Gas Laws Introduction: Although we cannot see gases, we can observe their behavior and study their properties. For example, we can watch a balloon filled with helium gas floating in air and conclude that
More informationIt s Cold Outside: Exploring the Effects of Temperature on GloFish Activity
Name Class Period Date It s Cold Outside: Exploring the Effects of Temperature on GloFish Activity Objective The learner will determine how a low temperature will affect the behavior of a GloFish fluorescent
More informationStudents measure the change in pressure by varying the volume of trapped air in a syringe while:
How Does a Trapped Gas Behave? Teacher Information Objective Students investigate the effect of changes in the volume of a confined gas on pressure at constant temperature. Using the pressure sensor, students
More informationExperiment 18 Properties of Gases
Experiment 18 Properties of Gases E18-1 E18-2 The Task In this experiment you will investigate some of the properties of gases, i.e. how gases flow, their phase changes and chemical reactivity. Skills
More informationClean toilet plunger Sensor extension cable. Add this important safety precaution to your normal laboratory procedures:
How Does a Trapped Gas Behave? Student Activity Worksheet Driving Question How does a change in volume of a confined gas affect its pressure? Materials and Equipment For each student or group: Data collection
More informationBASIC LABORATORY TECHNIQUES (Revised )
BASIC LABORATORY TECHNIQUES (Revised 1-6-13) A. WEIGHING The determination of the quantity of matter in a sample is most directly determined by measuring its mass. The process by which we determine the
More informationmass of container full of air = g mass of container with extra air = g volume of air released = cm 3
1992 Q32 The air pressure inside the passenger cabin of an airliner is 9 x 10 4 Pa when the airliner is at its cruising height. The pressure of the outside atmosphere at this height is 4 x 10 4 Pa. Calculate
More informationEvaluation copy. Interdependence of Plants and Animals. computer OBJECTIVES MATERIALS
Interdependence of Plants and Animals Computer 14 Plants and animals share many of the same chemicals throughout their lives. In most ecosystems, O 2, CO 2, water, food and nutrients are exchanged between
More informationCONCEPTUAL PHYSICS LAB
PURPOSE The purpose of this lab is to determine the density of an unknown solid by direct calculation and by graphing mass vs. volume for several samples of the solid. INTRODUCTION Which is heavier, a
More informationLab 4: Transpiration
Lab 4: Transpiration Water is transported in plants, from the roots to the leaves, following a decreasing water potential gradient. Transpiration, or loss of water from the leaves, helps to create a lower
More information11.1 Dumas Method - Pre-Lab Questions
11.1 Dumas Method - Pre-Lab Questions Name: Instructor: Date: Section/Group: Show all work for full credit. 1. If a 275-mL gas container has pressure of 732.6 mm Hg at -28 C, how many moles of gas are
More informationLABORATORY INVESTIGATION
LABORATORY INVESTIGATION MEASURING THE RATE OF PHOTOSYNTHESIS Light and Photosynthesis About 2.5-3 billion years ago a new chemical process, photosynthesis, was evolved by a unicellular life form. This
More informationSTAGE 2 BIOLOGY PHOTOSYNTHESIS TASK SHEET BASIC METHOD
STAGE 2 BIOLOGY PHOTOSYNTHESIS TASK SHEET BASIC METHOD Materials: Fresh green leaves (e.g. Ivy) 50mL plastic syringe 50mL measuring cylinder 2x100mL glass beaker distilled water 6% sodium bicarbonate solution
More informationAdaptations of Desert Plants
Adaptations of Desert Plants Topic The shape and surface area of a leaf are related to its ability to gain and release water. Introduction Deserts are biomes that present great difficulties to their inhabitants.
More informationBottle Rocket Launcher P4-2000
WWW.ARBORSCI.COM Bottle Rocket Launcher P4-2000 BACKGROUND: The Bottle Rocket Launcher allows for the exploration of launching rockets using commonly available materials such as plastic soda bottles and
More informationHeat-Trapping Gases Lab
Heat-Trapping Gases Lab Before performing the lab, show this video to the students. http://spark.ucar.edu/greenhouse-effect-movie-scott-denning Objective: The main goal of this activity is instrumentally
More informationResearch Question How does the concentration of catalase affect the speed of the decomposition reaction of Hydrogen Peroxide into oxygen and water?
Research Question How does the concentration of catalase affect the speed of the decomposition reaction of Hydrogen Peroxide into oxygen and water? Aim To observe the effect of increasing enzyme (catalase)
More informationGases. Unit 10. How do gases behave?
Gases Unit 10 How do gases behave? Gases are perhaps the most mysterious of all of the phases of matter. For the most part gases are invisible to us, and it was once believed that in the air there is no
More informationProperties of Gases Observing Atom Pressure of a Gas Measuring Gas Products of Chemical Inferring Molecule Reactions
It s a Gas! In a gas, molecules or atoms move constantly and spread far apart. If a gas cannot escape its container, it applies pressure on the container. For example, gas pressure inflates a balloon.
More informationThe Gas Laws: Boyle's Law and Charles Law
Exercise 6 Page 1 Illinois Central College CHEMISTRY 130 Name The Gas Laws: Boyle's Law and Charles Law Objective The simple laws governing the properties of gases can be readily demonstrated experimentally.
More informationSRI Model H2-40 Hydrogen Generator Operation and Maintenance Nov 2006
Nov 2006 Place the H2-40 on a benchtop surface which will not be damaged by water in the event of a leak. Fill a bottle with clean water. Tapwater is OK in most large cities, but bottled drinking water
More informationLab Session #4 AN Physical Properties
Lab Session #4 AN Physical Properties The main goal of this laboratory session is to provide a practical experience in the determination of the physical properties for AN and ANFO. The students will determine
More informationFlorida Method of Test for MEASUREMENT OF WATER PERMEABILITY OF COMPACTED ASPHALT PAVING MIXTURES
Florida Method of Test for MEASUREMENT OF WATER PERMEABILITY OF COMPACTED ASPHALT PAVING MIXTURES 1. SCOPE Designation: FM 5-565 1.1 This test method covers the laboratory determination of the water conductivity
More informationConclusions: 1. What happens to the volume of the gas inside the dropper as you put pressure on the container?
Gas Stations Chemistry Gas Station 1 Pressure Purpose: To observe the affect of pressure of a gas Safety: Wear goggles and aprons!!! Obtain an empty canister. Fill it half way with water and ½ an alka
More informationNAME BLOCK Density Lab PROBLEM: How can we determine the densities of different substances?
NAME BLOCK Density Lab PROBLEM: How can we determine the densities of different substances? PART 1 Determining relative density procedure 1. Designate an eyedropper for each beaker. Do not mix them up
More informationLocation and Use of Safety Equipment. Extinguisher Shower Fire Blanket
Lab Safety General Guidelines No food or drink at any time Never work alone Follow all teacher instructions Read Lab Procedures before performing lab Keep work areas and aisles clear No HORSE PLAY! Location
More informationGas Laws: Boyle s and Amonton s Laws MCTC Chemistry v.9.17
Gas Laws: Boyle s and Amonton s Laws MCTC Chemistry v.9.17 Objective: The purpose of this experiment is confirm Boyle's and Amontons' Laws in the laboratory. Prelab Questions: Read through this lab handout
More informationSTRUCTURED INQUIRY: Investigating Surface Area to Volume Ratio in Cells
STRUCTURED INQUIRY: Investigating Surface Area to Volume Ratio in Cells Introduction: All organisms are composed of cells. The size and shape of a cell determines how well it can deliver nutrients to its
More informationPURDUE UNIVERSITY TEFLON CORE PCB THRU-HOLE PREPARATION FOR BLACK HOLE APPLICATION RF LAB. Professor in charge: William Chappell MSEE 289
RF LAB Professor in charge: William Chappell MSEE 289 AUTHOR: BOB SALISBURY DATE: 12/5/2007 12/6/2007 page 1 of 7 TABLE OF CONTENTS 1 SCOPE... 3 2 PURPOSE... 3 3 REFERENCE DOCUMENTS... 3 4 MATERIALS...
More informationCLASS COPY-DO NOT WRITE ON
Save Our Shells - Central Question: How does carbon dioxide affect salt water? CLASS COPY-DO NOT WRITE ON Overview of experiment: Exhaling carbon dioxide into a beaker of salt water mimics the gas exchange
More informationObjective To identify a pure liquid substance using the physical properties of solubility, density, and boiling point.
Chemistry 1020 Identification of an Unknown Liquid Objective To identify a pure liquid substance using the physical properties of solubility, density, and boiling point. Text reference solubility, density,
More informationAnalysis of a KClO3 Mixture and Determination of R
Experiment 10 Analysis of a KClO3 Mixture and Determination of R Pre-Lab Assignment Before coming to lab: Read the lab thoroughly. Answer the pre-lab questions that appear at the end of this lab exercise.
More informationCooling Gases Phase Changes and Phase Diagrams
Cooling Gases Phase Changes and Phase Diagrams SCIENTIFIC Introduction What happens to gases when the temperature is dropped way down? As this activity illustrates, it all depends on the gas. Concepts
More informationRead ENTIRE lab up to Disposal Section. MAKE NOTES!!! **For Procedures, Highlight equipment used and circle quantities measured out.
Lab Ch 2 Mass, Volume, & Density Lab Partners: READ Prelab!!! Read ENTIRE lab up to Disposal Section. MAKE NOTES!!! **For Procedures, Highlight equipment used and circle quantities measured out. Density
More informationCHEM 321 Experiment 1
CHEM 321 Experiment 1 Basics Review and Calibration of Volumetric Glassware There are three types of containers used in lab to contain or deliver liquids: volumetric, ordinary and disposable glassware.
More informationMODELING RADIOACTIVE DECAY WITH FLUID DYNAMICS
DEVIL PHYSICS BADDEST CLASS ON CAMPUS MODELING RADIOACTIVE DECAY WITH FLUID DYNAMICS Note: Due to material and space constraints, you will work in teams of three to collect data Each individual will be
More informationThe Ideal Gas Constant
Chem 2115 Experiment # 8 The Ideal Gas Constant OBJECTIVE: This experiment is designed to provide experience in gas handling methods and experimental insight into the relationships between pressure, volume,
More information