Multiplex screening solutions for Growth Promoters and Synthetic Steroids

Transcription

1 Multiplex screening solutions for Growth Promoters and Synthetic Steroids DISTRIBUIDOR / DISTRIBUTOR: ww

2 Multiplex Drug Residue Screening Solution Randox present the worlds only multi-analyte semi-quantitative Drug Residue analyser. The use of the Evidence Investigator allows simultaneous detection of multiple analytes from a single sample. Biochip Array Technology Benefits: Massive cost savings and minimal consumable costs Less labour (Minimal labour costs could be reduced by at least 5 fold by employing multiplex biochip arrays) Faster Turnaround Time Higher Throughput (45 samples or 675 tests in under 2 hours) Faster Adaptability to new regulations and new drug residue targets (up to 65 new antibodies in development) APPLICABLE TO MULTIPLE MATRICES Feed Urine Tissue Honey Milk IDEAL FOR DRUG RESIDUE LABORATORIES Multiplex screening for Veterinary Drug Residues Consolidation of testing to provide multiple test results from a single sample No requirement for deuterated standards Requires minimum technical expertise Same sample preparation method can be applied across a number of our arrays Extensive menu of drug residue tests developed

3 Growth Promoter Arrays Growth-promoting drugs have been used by the livestock industry for over 30 years to improve an animal s ability to efficiently use nutrients and produce leaner, more affordable meat. Concerns over the use of hormones in food-producing animals first arose when a number of incidents linked hormone residues in meat with various medical conditions after consumption. As a result, the use of growth-promoting hormones in livestock production is either prohibited or strongly regulated. Compliance with these regulations is ensured by national monitoring programmes. Randox provides a reliable and cost effective screening solution for multiple growth promoters, which conforms to all major regulatory requirements. Simultaneous measurement of multiple growth promoter classes in a single sample GROWTH PROMOTER ARRAYS Growth Promoter b-agonists Rapid Urine Screen Array Boldenone (EV3521) Corticosteroids Ractopamine Growth Promoter b-agonists Multiple Matrix Screen Array Boldenone (Urine, Tissue, Feed) (EV3726) Corticosteroids Nandrolone Ractopamine Stilbenes Urine Urine Tissue Feed GROWTH PROMOTER KEY BENEFITS: Comprehensive screening panel for growth promoters Urine, tissue and feed applications Choice of rapid dilution or Immunoaffinity column method with enhanced LODs* for Urine Rapid analysis; simple sample dilution Excellent analytical performance Excellent generic antibodies * Limits of Detection

4 Sample preparation Multi-analyte sample preparations for urine, tissue and feed are provided for use with this unique multiplex system. Due to the nature of this collection of growth promoter compounds, multiple sample preparations would be required. Randox however offer one single preparation to cover all 9 of these compounds (35 including cross reacting analytes). The methods provide a simple clean up without the use of costly solvents. URINE PREPARATION Dilute centrifuged urine 10 fold in assay diluent and apply directly to the Biochip MULTIPLE MATRIX SCREEN From 5ml of a centrifuged and filtered urine Deconjugation and neutralisation Dilution and centrifugation TISSUE PREPARATION From 1g of homogenised sample Addition of neutral aluminium oxide along with methanol/1m NaOH Sample is vortexed, rolled and centrifuged Supernatant is decanted Process repeated with remaining pellet Supernatants are frozen, centrifuged and diluted with column wash buffer FEED PREPARATION From1g ground feed sample Addition of methanol/h2o Sample is vortexed and centrifuged Supernatant is decanted Addition of extraction buffer vortex and centrifugation Supernatant is decanted 125µl of each supernatant are then combined and diluted in wash buffer SAMPLE IS NOW READY FOR APPLICATION TO THE IMMUNOAFFINITY COLUMN SAMPLE PURIFICATION - IMMUNOAFFINITY COLUMNS (IAC) Sample clean up is an essential part of drug residue testing, whether it is performed by ELISA, LC/MS or any other technique. To complement the Randox range for measuring drug residues, Randox offers a wide range of immunoaffinity columns for sample purification. The Randox Growth Promoter Immunoaffinity column ensures rapid sample purification reducing analysis time. RANDOX IAC BENEFITS: Very efficient and highly specific THE PRINCIPLE OF SAMPLE PURIFICATION BY IMMUNOAFFINITY COLUMNS Addition of crude sample Removal of interferents by washing step Elution of bound analyte KEY Analyte Antibody specific for analyte Interferent Simultaneous sample clean up Higher analyte recovery Improved safety with reduced waste and disposal costs Faster due to fewer purification steps Can be automated Convenient available in packs of 5 with buffers Cost-effective each column can be used up to 10 times Purified sample ready for further analysis Long shelf life 2 years from date of manufacture

5 Growth Promoter Analytical performance: Limits of Detection (LOD) Growth Promoter Rapid Urine Screen Array (EV3521) Analyte b-agonists 0.74 Boldenone 1.83 Corticosteroids 0.91 Ractopamine LOD* (ppb) 06/503/GP *Limit of Detection (LOD) = Mean +3SD, results include assay dillution factor. Growth Promoter Multiple Matrix Screen Array (EV3726) Analyte Urine LOD* (ppb) Tissue LOD* (ppb) Feed LOD (ppb) b-agonists Boldenone Corticosteroids Nandrolone Ractopamine Stilbenes /063, 077/384, 09/196, 197, 198, 202, 204, 205/384, 09/479, 480, 483, 493/295 * Limit of detection (LOD) = mean +3SD SPECIFICITY Analyte Growth Promoter Specificity* b-agonists Clenbuterol Mapenterol Carbuterol Salbutamol Cimbuterol Mabuterol Terbutaline Methyl-clenbuterol Pirbuterol 113% 104% 70% 54% 41% 22% 20% 15% Boldenone Corticosteroids 17b Boldenone 1,4 Androstadiene-3,17-dione 17a Boldenone Boldenone glucuronide Dexamethasone Betamethasone-21-acetate Flumethasone Betamethasone Dexamethasone-21-acetate Nandrolone 19-Nortestosterone (17β) 19-Nor-4 androstene,3, 17-dione acetate 17β 19-Nortestosterone (17β) sulphate 19-Nortestosterone (17α) 19-Nortestosterone β glucuronide *06/466, 470, 476, 485, 488, 489, 490, 504, 505, 507/GP Standardised material. 55% 15% 15% 133% 57% 31% 27% 143% 109% 70% 55% 27% 26% Analyte Growth Promoter Specificity* Ractopamine Ractopamine Ractopamine hydrochloride Stilbenes 16b Hydroxystanozolol Hexestrol Diethylstilbestrol glucuronide Diethylstilbestrol Dienestrol 17b 17a α - Zearalenol β - Zearalanol Zearalenone Zearalanone 45% 289% 105% 72% 21% 10% 5.3% <1.4% <0.4% 315 tests over 7 analytes in 2 ½ hours (rapid urine screen) 405 tests over 9 analytes in 2 ½ hours (multiple matrix screen) Typical calibration range: 0-5 ppb Typical intra-assay precision: CV 10% at around 2 ppb Typical inter-assay precision: CV 15% at around 2 ppb

6 Synthetic Steroids Array Synthetic steroids are used in food producing animals as growth promoters to produce leaner, better quality meat. However, the use of these steroids is prohibited in food producing animals as they have been found to have damaging health implications in humans. Therefore accurate screening of synthetic steroids is crucial. SYNTHETIC STEROIDS ARRAY Synthetic Steroids Array 17b-Clostebol/ CLAD (Urine) (EV3694) Ethinylestradiol Gestagens (generic) Methandriol Methyltestosterone Urine Randox provide a reliable and cost effective screening solution for synthetic steroids. KEY BENEFITS: Simultaneous measurement of multiple synthetic steroids from a single sample, saving time and resources Suitable for both bovine and equine samples Broad recognition of Gestagens Excellent analytical performance Simple Solid Phase Extraction method Conforms to regulatory requirements Specificity/Cross-reactivity (CR) Assay Targets Detected %CR 17β-Clostebol 4-Chloro-androsten-3, 17-dione (CLAD) 17β-Clostebol 34.8% Ethinylestradiol Ethinylestradiol Gestagens Chlormadinone acetate Medroxyprogesterone acetate Megestrol acetate Melengestrol acetate 149.3% 105.1% 56.9% Methandriol Methandriol Methyltestosterone 08/131, 147, 154/304 Methyltestosterone Methylboldenone Limit Of Detection (LOD) 40.7% Assay Equine Urine LOD** (ppb) Bovine Urine LOD** (ppb) 17β-Clostebol Ethinylestradiol Gestagens Methandriol Methyltestosterone /153/304, 09/217/304 *This is a typical calibration range and may vary slightly with the batch of calibrators **Following sample purification by solid phase extraction

7 Drugs of Abuse and Veterinary Drug Residue Arrays ANTIMICROBIAL ARRAYS ANTHELMINTICS ARRAY (EV3772) Aminobenzimidazoles Avermectins Benzimidazoles Levamisole Moxidectin Thiabendazole Triclabendazole ANTIMICROBIAL ARRAY I (EV3523) Sulphachlorpyridazine Sulphadiazine Sulphadimethoxine Sulphadoxine Sulphamerazine Sulphamethazine Sulphamethizole Sulphamethoxazole Sulphamethoxypyridazine Sulphamonomethoxine Sulphapyridine Sulphaquinoxaline Sulphathiazole Sulphisoxazole Trimethoprim ANTIMICROBIAL ARRAY II (EV3524) Ceftiofur Florphenicol/ Thiamphenicol Quinolones (generic) Streptomycin/Dihydrostreptomycin Tetracyclines (generic) Tylosin/ Tilmicosin ANTIMICROBIAL ARRAY III (EV3695) AHD AMOZ AOZ SEM Chloramphenicol DRUGS OF ABUSE ARRAYS DRUGS OF ABUSE ARRAY I ( EV URINE EV WHOLE BLOOD EV ORAL FLUID EV URINE SQ) Amphetamine Barbiturates Benzodiazepine 1 Benzodiazepine 2 Cannabinoids Cocaine Methadone Methamphetamine Opiates Phencyclidine DRUGS OF ABUSE ARRAY I PLUS ( EV URINE EV WHOLE BLOOD) Amphetamine Barbiturates Benzodiazepine 1 Benzodiazepine 2 Buprenorphine Cannabinoids Cocaine MDMA Methadone Methamphetamine Opiates Phencyclidine Tricyclic Antidepressants (TCAs, generic) DRUGS OF ABUSE ARRAY II ( EV URINE EV WHOLE BLOOD) Buprenorphine Fentanyl Generic Opioids Ketamine LSD MDMA Methaqualone Oxycodone 1 Oxycodone 2 Propoxyphene GROWTH PROMOTERS ARRAYS GROWTH PROMOTER RAPID URINE SCREEN (EV3521) b-agonists Boldenone Corticosteroids Ractopamine GROWTH PROMOTER MULTIPLE MATRIX SCREEN (EV3726) b-agonists Boldenone Corticosteroids Nandrolone Ractopamine Stilbenes SYNTHETIC STEROIDS ARRAY (EV3694) 17b-Clostebol Ethinylestradiol Gestagens (generic) Methandriol Methyltestosterone FUTURE DEVELOPMENTS b-agonists Array Coccidiostats Array Drugs of Abuse Array III Drugs of Abuse Array IV Anti-Inflammatory Drugs Array

8 Publications Examples of Randox Poster Presentations Development of a rapid sample preparation method for the extraction of 85 drug residues in feed matrices. Rabiller, D., et al. 6th International Symposium on Hormone and Veterinary Drug Residue Analysis, Simultaneous determination of synthetic steroids using biochip array technology. Finnegan, C., et al. ICRAV Simultaneous measurement of growth promoters with biochip array technology. Finnegan, C., et al. ICRAV Multiplex screening for Veterinary Drug Residues The ultimate cost saving Drug Residue Screening solution Randox Laboratories Limited, 55 Diamond Road, Crumlin, County Antrim, BT29 4QY, United Kingdom T +44 (0) F +44 (0) E drugresidues@randox.com I AUG10 LT170 Information correct at time of print. All Randox products made in the UK. Randox Laboratories Limited is a company registered within Northern Ireland with company number N.I VAT Registered Number: GB Product availability may vary from country to country. Please contact your local Randox representative for information.