Developing a genetic baseline for Chinook salmon in the Copper River. Alaska Department of Fish and Game Native Village of Eyak National Park Service

Transcription

1 Developing a genetic baseline for Chinook salmon in the Copper River Alaska Department of Fish and Game Native Village of Eyak National Park Service

2 Questions What is the genetic diversity of Chinook salmon in the system?

3 Questions What is the genetic diversity of Chinook salmon in the system? What is the migration timing of Chinook salmon stocks in the Copper River system?

4 Questions What is the genetic diversity of Chinook salmon in the system? What is the migration timing of Chinook salmon stocks in the Copper River system? What is the composition of Chinook salmon stocks in ocean fisheries near the Copper River delta?

5 Objectives 1. Develop DNA database to identify spawning populations use SNPs and microsatellites contribute to species-wide database 2. Investigate run-timing and entry patterns within the river 3. Investigate contributions to ocean fisheries

6 Microsatellite markers simple sequence repeats - Allele designations related to fragment size Fish 2 Fish Fish 4 Fish 3

7 SNP markers single nucleotide polymorphism - Alleles designate a single base change G C G C Fish 1 What SNP? A, C, G or T? Fish 2

8 Field Collections

9 Total Sampling Effort 2003 Baird Canyon Fishwheel (radio tags) Baird Canyon Fishwheel (radio tags). 296 Tributary Spawners....1, Tributary Spawners Cordova Ocean Fishery Samples 1,612 Baird Canyon Mixture Samples 1,418 Project Total.5,232

10 Copper River Chinook Sampling Sites Middle Fork West Fork Gulkana R Chistochina River Paxson Fork Gakona River Sinona Creek Slana River, Bone Ck East Fork Indian Creek Upper Copper R 2004 Mendeltna Creek Tazlina R Kiana Creek Chetaslina River St. Anne Creek Klutina R Manker Creek Tonsina R Chitina R Nizina River Tebay River Greyling Creek Valdez Bremer River Tasnuna River Baird Canyon Miles Lake

11 Copper River Chinook Sampling Sites Middle Fork West Fork Gulkana R Chistochina River Paxson Fork Gakona River Sinona Creek Slana River, Bone Ck East Fork Indian Creek Otter Cr. Upper Copper R 2005 Mendeltna Creek Tazlina R Kiana Creek Chetaslina River St. Anne Creek Klutina R Manker Creek Tonsina R Chitina R Nizina River Tebay River Greyling Creek Valdez Bremer River Tasnuna River Baird Canyon Miles Lake

12 Numbers of fish Collections Tributary Spawners Baird 03 = 288 Baird 04 = 296 Trib 04 = 1,266 Trib 05 = 352 Total = 2,202 0 Chitina (Tebay) 1 Tonsina Klutina Tazlina Gulkana Upper Copper Tributary 6

13 Laboratory Analysis

14 -0.05 Multidimensional Scaling, 31 SNPs Upper Copper Gulkana Middle Lakes Chitina 1 4 Dimension Tonsina Tazlina Klutina Tazlina (Mendeltna) Dimensio n Dimension 2

15 -0.05 Multidimensional Scaling, 31 SNPs Upper Copper Gulkana Mendeltna Middle Lakes Chitina 1 4 Dimension Tonsina Tazlina Klutina Tazlina (Mendeltna) Dimensio n Dimension 2

16 Dimension 1 0 Dimensi on Upper Copper Gulkana Mendeltna Middle Lakes Chitina Multidimensional Scaling, 13 MSATs Dimension

17 Dimension Upper Copper Gulkana Mendeltna Middle Lakes Chitina Major Genetic Lineages Dimensio n Miles Lake Dimension 2

18 *Copper River Basin Geology Proglacial Lake Lightly Modified Moraine Bed Rock Volcanic *Map Courtesy of Ecotrust

19 Result of 100% simulations Gulkana R. 96% 98% Mendeltna Cr. 97% Middle Lakes 99% Upper Copper 94% Chitina R. Baird Canyon Miles Lake

20 Klutina River Guide Samples Where does genetics put them? 2% Mendeltna 90% Middle Lakes Chitina R. Baird Canyon 8% Miles Lake

21 Gulkana River Guide Samples Where does genetics put them? Upper Copper R 99% Gulkana R 1% Chitina R.

22 What Next? Finishing touches on baseline -Evaluate radio tag samples for use in baseline -Evaluate guide samples for use in baseline Investigate run timing using Baird Canyon mixture samples Perform mixed stock analysis on Cordova ocean fishery samples

23 Thanks Native Village of Eyak Ahtna LGL Eric Veach - National Park Service James Savereide ADF&G Sportfish Klutina River Association Guides Tom Taube ADF&G Sportfish

24 Tributary Sampling Results Location Total Chitina River Tebay River Tonsina Little Tonsina (J*) 20 NS 20 Little Tonsina Greyling Creek 16 NS 16 Klutina Main Stem (Guides) 168 NS 168 Manker Creek Tazlina Kaina Creek (J*) 133 NS 133 Kaina Creek 75 NS 75 Mendeltna Creek 144 NS 144 Gulkana Mainstem (Guides) 130 NS 130 Main Stem (ADFG) 46 NS 46 Middle Fork Paxson Fork Upper Copper Bone Creek Chistochina River 145 NS 145 Indian Creek Sinona Creek Ahtell/Slana Otter Creek NS Total Fish 1, ,618 *Juvenile Samples