Investigation into the patchy coastal distribution of Chromis chromis in Revellata Bay, Corsica

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1 Investigation into the patchy coastal distribution of Chromis chromis in Revellata Bay, Corsica Abstract Astrid Leitner and Stephanie Nguyen Chromis chromis is one of the most important species in the Mediterranean rocky littoral zone; it is not only the most abundant fish but also one of the few planktivorous water column feeders. We found that this species forms large aggregations in consistent areas, mainly over the transition zone where sloping granite meets Posidonia oceanica meadows. We also showed that these fish consistently school around the densest copepod aggregations available as predicted by the optimal foraging theory. We then attempted to correlate daily copepod fluctuations with the observation of these consistent plankton patches, but this yielded only preliminary results that highlight the need for further exploration. Introduction The distribution of marine organisms is highly variable and is often influenced by predator-prey interactions (Benoit-Bird 29). Many organisms rely on schooling and aggregated prey species for feeding (Benoit-Bird 29). A vital aggregated prey species in the marine environment are plankton. Recent advances have allowed us to investigate feeding behavior in relation to phytoplankton by utilizing vertical towing methods. This method, different from the standard horizontal tows, have allowed for scientists to observe plankton distribution within the water column by using nets that are capable of being towed vertically. This method has revealed that phytoplankton form high-concentrated smaller aggregations that stretch horizontally in the ocean called thin layers (Leising 21). These thin layers also contain organisms called zooplankton that exist in dynamic 3-dimensional structures. These structures are organized in pancake-like stacks that are thought to stimulate fish schooling (Benoit-Bird 29). In addition to these natural aggregations, feeding patterns of plankton that fluctuate daily also change their positioning within the water column throughout the day (Noda et al. 1992). To further investigate what encourages fish schooling, we chose to use the optimal foraging theory, which states that predators aggregate in areas of high prey densities (Krebs 1978). Previous studies of the effects of plankton distribution on fish schooling have been focused on larger scale patterns. The goal of our project was to test smallscale horizontal patchiness in zooplankton communities and the response of planktivorous fish to this 3-dimensional patchiness. We focused our study on the planktivorous fish Chromis chromis, the most common schooling fish in the Mediterranean Sea (Bracciali et al 212). The goals of this work were to: (1) observe fish schooling behavior; (2) determine whether fish accumulation was a result of plankton aggregation; (3) track diel fluctuations of copepod densities. The general

2 question we asked was: What factors influence the distribution of C. chromis schools? The specific questions that were asked were: (1) Are there areas where C. Chromis schools occur consistently? (2) Are these regions correlated to the densest plankton aggregations? (3) How do plankton densities fluctuate over a 24 hour cycle? Methods Study Site This study was conducted at the Station de Recherches Sous-Marines et Océanographiques (STARESO) located on the Mediterranean Sea near Calvi, France during the month of October, 212. All tows, surveys, and observations were made at depths ranging from the surface to 15 m and within 4 m of the mouth of the STARESO harbor with study sites both to the north and the south of the harbor. In this region, granitic walls and boulders drop steeply into the water, transitioning approximately 6 m in depth to flat substrate, mostly sand, covered by Posidonia oceanica, a seagrass endemic to the Mediterranean Sea and very common in this part of Corsica. Figure-1: This figure shows the island of Corsica and our study site at STARESO on the northwest side of the island. The last inlet shows our 9 survey transects and our larger study area.

3 Study Species In a study near the Island of Ischia, Chromis chromis was the most abundant fish species, comprising 38% of all fish collected (Zupo et al. 21). In another area of the Southern Adriatic Sea, Fasola et al. (1997) found C. chromis made up 52% of observed fish species. The sheer abundance of the species, despite its small size, makes C. chromis a key species in the coastal ecosystem of the western Mediterranean Sea (Bracciali et al. 212). C. chromis through the process of consumption and excretion stimulate nutrient transport from the pelagic zone by driving down the carbon cycle to the benthic system in the water column. This nutrient export is the reason why C. chromis are important to the Mediterranean ecosystem. Reaching up to 25 cm in length, with an average length of 13cm, C. chromis form large dense schools that exhibit diel feeding patterns (Bracciali et al. 212, Loris et al. 199). Schools feed in the water column during daytime and resting at night within the shelter of rocks. These patterns are variable and are affected by the amount of light attenuation observed daily (Bracciali et al 212). School Distribution In order to test the idea that schools were patchily distributed and that certain locations supported consistent schools, a series of parallel 3 m fish transects were run twice per day at specific times in three regions (1) the north side of the STARESO harbor (2) the mouth of the harbor, and (3) the south side of the harbor. All transects were conducted from a 9 N bearing in the late morning and late afternoon. Late morning transects were run between 9 and 11AM, and late afternoon transects were performed between 2-5PM. Presence or absence of fish schools were marked along each meter, and a map of the school distribution was developed for each time by measuring the length of the schools and estimating the horizontal extent the approximate area along the transect where the schools were observed. The physical conditions and the vertical location of the patches within the water column were also noted. If the average depth of the school was within 2m of the bottom the school was considered over the bottom; any other school was marked as within the water column. Patch area was calculated instead of school density because the large number of individuals in each school was too big to accurately estimate. Plankton Tows For our study we conducted phytoplankton tows at various depths with a 2- micron phytoplankton net. All surveys were completed using SCUBA, swimming either north or south of the harbor and searching for large, dense schools of feeding planktivorous fish. A picture of every school was taken before sampling, and the

4 depth of the middle of the school was noted as well as the presence or absence of other schooling species in the sampling and their depths. We would then run out a weighted transect tape along the length of the fish school and tow through the school for a minimum of 6m. The first tow would always be done directly through the school. The net would then be twisted shut and brought to the surface where the sample would be initially processed. This was done by removing the cot-end and emptying the contents into a cleaned jar. The cot-end was then rinsed several times with filtered seawater and emptied into the same sample jar. The cot-end was then reattached onto the plankton net, twisted shut, and brought back down to the depth of the school. Subsequent tows would then be conducted above, below, right, and/or left depending on the positioning of the school relative to the geomorphology of the sample area. Horizontal tows were always at least 5m from the edge of the school to ensure no overlap. Tows above and below the school were only conducted if the school was in water deep enough and the school was compressed enough to make it possible to tow without overlap. All samples were preserved in 2.5% formalin within two hours of sampling. The samples were filtered down to less than 5 ml using the cod-end of the phytoplankton net. For processing, the exact volume of the sample was determined by pouring the filtered down vial into a graduated cylinder. Then two 1mL subsamples were extracted from the homogenized samples. The zooplankton within each subsample were counted under a dissecting microscope using broad categories: copepods, mollusks, larva, amphipods, chaetognaths, and cladoceres. Nauplii were counted as copepods. These large categories were chosen because the goal of quantifying zooplankton was not to note compositional changes in the plankton community but merely to quantify the concentration of prey items with schools relative to surrounding water. The total number of zooplankton in each sample was extrapolated from the average number of each category between the two subsamples. This total was then divided by the total volume of water sampled by the plankton tow (the area of the net opening multiplied by the total length of the each tow). Fluctuations in Prey Availability To test the hypothesis that the consistently present schools take advantage of a relatively permanent local concentration of copepods, we sampled one area two hours after sunset, just before sunrise, and every three hours subsequently for 1 hours. We chose this region based on the results of our surveys, choosing one transect based on a region of significantly higher than expected presence of C. chromis schools and another transect 1 m offshore from the first in an area of lower than expected presence of C. chromis. These samples were processed using an identical methodology to the one described in the plankton tows section.

5 Statistical Analyses In order to get a picture of the distribution of C. chromis schools, we first found the overall probability of a school being at each meter mark within the grid. We then ran a binomial probability analysis to determine what number of successes is counted as a significant number of positive and negative deviations from the overall expected probability of school presence. From this calculated value we created a map of our sample area from expected, greater than expected, and less than expected regions. In order to test the hypothesis that the clearly defined vertical and horizontal location and orientations of the C. chromis schools were associated with the densest aggregations of prey items in the region as predicted by the optimal foraging theory, we ran ANOVA tests on the plankton tows done within the schools, vertically outside the schools, and horizontally outside the schools. In order to get a picture of the distribution of C. chromis schools, we first found the overall probability of a school being at each meter mark within the grid (.279). We then ran a binomial probability analysis to determine what number of successes is counted as a significant number of positive and negative deviations from the overall expected probability of school presence (.556). From this calculated value we created a map of our sample area from expected, greater than expected, and less than expected regions. Results School Distribution As shown in figures one and two, schools were generally more likely to be found near the rocks and less likely to be found further offshore over the Posidonia oceanica. Schools were consistent from to 7 meters along each transect in our sampled grid with the noteworthy exception at the 3 S transect. This transect was slightly different than the others as it began at the Posidonia oceanica meadow cobble interface instead of at the foot of the sloping granite. This occurred due to the position of the transect at the mouth of a small bay lined on either side by granite slopes (sampled by the 2 S and 4 S transects). Another notable exception to the general pattern was a consistent school at the offshore end of the furthest northern transect. There was an interesting feature on the benthos at this point. Here there was a ledge approximately two meters in height formed by disturbances to the Posidonia oceanica meadow. Stability of Schools

6 3 25 Metermark a) Transect (meters) b) Figure 2: a) On this chart.279 is the overall probability of encountering a school on our sampled grid. The red meter marks represent those marks that have a significantly higher probability of schools than the overall probability, and the blue meter marks highlight which regions have a significantly lower probability of schools. b) This is a three dimensional visualization of the same statistical data where red areas represent the highest probability of encountering a school and blue areas represent the lowest probability. This image is oriented so that the observer is looking into shore with the negative transects representing the southern transects and the positive ones representing the northern transects. Meter mark is closest to shore and meter mark 4 is furthest offshore. The Y axis shows the probability of encountering a Chromis school. Plankton Tows We found that copepod densities were significantly higher within schools than outside C. Chromis schools (see Fig 3a; p-value =.11). We also found that copepod densities were highest within schools, slightly lower on average below

7 schools, lower to the side of schools, and lowest above schools (2588, 283, 2245, 1138). 5, Copepod Densities 5, Copepod concentration 4, 3, 2, 1, Copepod concentration 4, 3, 2, 1, a) Location (relative to school) b) Type III Tests for Fixed Effects In Out In Side above below Location (relative to school) Effect Numerator df Denominator df F-Ratio p-value Code Figure 3: a) This chart shows the average densities of copepods per square meter inside schools and outside schools with standard error bars. b) This chart shows a more detailed view of copepod densities relative to school locations. The side category is a combination of left and right tows. Fluctuations in Prey Availability During an exploratory one-day trial where five tows were conducted within a 24-hour period, we found a peak of copepod.

8 35 Diel Chromis and Copepod Patterns 1.2 Copepods per square meter Copepod Numbers Chromis Abundance 21:3 7:3 11:3 2:3 5:3 Time of Day Figure 4: This graph shows the average number of copepods per square meter during each Chromis Presence Discussion School Distribution We found consistent schools of C. chromis day after day hugging the geomorphology of the coastline and much less stable aggregations out in mid-water over the Posidonia oceanica meadows. We believed this could have important implications for the nutrient flux supporting the diverse benthic assemblages that exist under regions of the most consistent schools of C. chromis. C. chromis were found to school consistently over the rock-meadow interface feeding on copepods and excreting nitrogen, phosphorous, and carbon (Pinnegar et al. 27). Pinnegar et al. found that via respiration, excretion, and feces, one 1.25 g C. chromis releases 37.2 mg/day carbon, 8.89 mg/day nitrogen, and 1.7 mg/day of phosphorous (27). Therefore large aggregations of C. chromis can drastically change nutrient levels down current of their consistent schooling locations. In fact schools of the closely related planktivorous damselfish C. punctipinnis have been shown to consistently increase ammonium levels down stream of their aggregations (Bray 1986). Ammonium is crucial to the growth many primary producers such as macroalgae[al1]. Moreover, many Mediterranean phytoplankton are phosphorous limited making the phosphorous excreted by these schools even more important to the base of the food web (Pinnegar 27[AL2] ). Additionally, due to their diel feeding pattern, C. chromis also bring nutrients back to their nighttime shelters within the rocks (Pinnegar 27). These shelter areas can house 5-1 individuals per square meter (Pinnegar 27). The elevated nutrients here could serve to

9 support the diverse invertebrate and algae communities that exist on the underside of these boulders and rocks[al3] (Beltran et al in prep). As the most abundant fish in terms of both number and biomass in the Posidonia system, the nutrient fluxes induced by C. chromis schools could be crucial to the benthic communities thriving under consistent schooling locations (Pinnegar et al 25, 27; Bracciali et al. 212). Plankton Tows Copepod densities were found to be significantly higher within C. chromis aggregations than outside the schools as predicted by the optimal foraging theory which maintains that predators will forage longer on denser prey patches to maximize energy intake. It is interesting to subsequently consider why there are consistent copepod aggregations in these areas in the water column over the rock/meadow interface. We attempted to test whether water motion set up a sort of entrapping eddy that focused the zooplankton there; however, when we attempted to measure the current with a handheld flow-meter the water motion was too slight to detect anywhere along our 3 m transect (3m directly offshore starting from the rock edge). Even when attempting to qualitatively observe water motion by releasing dyes into the water column, no directional flow was detectable. While the plankton aggregation could still be maintained by the physical processes occurring at the water s edge, it may also be maintained by biological processes. The C. chromis schools excrete nutrients into the water, including phosphorous, an important limiting nutrient for the region s phytoplankton (Pinnegar et al. 27). This could cause very small scale phytoplankton blooms in precisely the areas that C. chromis aggregate around to feed. This in turn would result in copepods maintaining their position in these regions as well, perpetuating the local consistent C. chromis aggregations. This hypothesis is purely speculation derived from our observations and the energy budget analysis done by Pinnegar et al. in the same region; it would be an interesting future project to consider. Another added benefit that C. chromis derive from feeding above the interface zone is proximity to shelter. Almost daily during our school surveys we observed pairs of hunting jack species (Serioli lalandi) swimming through the C. chromis schools. The C. chromis responded by swimming down toward the boulders for shelter. In fact, one group of jacks could make an entire school disappear momentarily into the rocks only a few meters away emerging seconds to minutes later to reclaim their previous feeding position. Schools over Posidonia oceanica meadows seemed to have a much longer response time, often needing to swim five meters or more to hide on the benthos within the seagrass and taking longer to regroup afterwards. Again this needs to be examined further and tested empirically. Fluctuations in Prey Availability Our results showed that C. chromis presence did not mirror copepod densities as expected. In fact they appear be directly opposite. However, it was to be expected that there were no copepods during the night tow or the early morning tow because of the diel feeding pattern of the C. chromis despite the high density of copepods during this time. The peak density of C. chromis actually corresponded to the lowest

10 copepod density however, because this density was less than half of all other densities recorded at that time of day (collected during the daily tows), we believe that this is not an accurate view of copepod densities for this time of day. This could be confirmed if weather had allowed for multiple sampling days for this question. The highest copepod density in this location occurred at 2:3 in the afternoon, a time period where many schools of C. chromis had been observed previously. Again the lower C. chromis presence here is most likely an artifact of our insufficient sample size. Pinnegar et al reports a general behavior of zooplankton in the bay of Calvi where plankton rise to the surface at dusk and remain in the surface waters until dawn where they sink to their day depth; however, this behavior may not apply to the shallow coastal system we are working in. Conclusion C. chromis are the most abundant fish in the Posidonia oceanica community. During daylight hours they form large schools that feed on dense copepod aggregations influencing the nutrient levels in these localized regions as well as the nutrient levels in their night-time resting places. We found schools consistently aggregated over the interface between the Posidonia oceanica meadows and the rocky slope. This may be due to food availability as well as proximity to shelter. Copepod densities were significantly higher within schools than outside of schools. This could be due to entrapment and retention of plankton in this region by physical processes or maintained by biological processes. C. chromis presence did not mirror copepod densities as expected but this may be an artifact of limited sample size. Many questions and hypotheses remain to be examined, as this species is crucial to the trophic web in this system. References Benoit-Bird, K. J. (29). Dynamic three-dimensional structure of zooplankton thin layers is affected by foraging fish. Mar. Ecol. Prog. Ser, 393, Bracciali C, Campobello D, Giacoma C, Sarà G (212). Effects of Nautical Traffic and Noise on Foraging Patterns of Mediterranean Damselfish (C. chromis C. chromis). PLoS ONE 7(7): e4582. doi:1.1371/journal.pone.4582 Bray, R.N., Purcell, L.J., Miller, A.C., (1986). Ammonia excretion in a temperate-ref community by a planktivorous fish, C. chromis punctipinnis (Pomacentridae) and potential uptake by young Giant Kelp, Macrocystis pyrifera (Laminales). Mar. Biol. 9: Fasola, M., Canova, L., Foschi, F., Novelli, O. and Bressan, M. (1997). Resource Use by a Mediterranean Rocky Slope Fish Assemblage. Marine Ecology, 18: doi: /j tb426.x

11 Krebs J.R. (1978). Optimal foraging, predation risk and territory defense. Ardea 68: Leising, A. W. (21). Copepod foraging in patchy habitats and thin layers using a 2- D individual-based model. Marine Ecology Progress Series, 216, Loris, D. and J. Rucabado (199). Pomacentridae. p In J.C. Quéro, J.C. Hureau, C. Karrer, A. Post and L. Saldanha (eds.) Check-list of the fishes of the eastern tropical Atlantic (CLOFETA). JNICT, Lisbon; SEI, Paris; and UNESCO, Paris. Vol. 2. Noda M., Kawabata K., Gushima K., Kakuda S. (1992). Importance of zooplankton patches in foraging ecology of the planktivorous reef fish Chromis chrysurus (Pomacentridae) at Kushinoerabu Island, Japan. Marine Ecology Progress Series 87: Pinnegar JK, Polunin NVC (26) Planktivorous damselfish support significant nitrogen and phosphorus fluxes to Mediterranean reefs. Mar Biol 148: Pinnegar JK, Polunin NVC, Videler JJ, de Wiljes JJ (27) Daily carbon, nitrogen and phosphorous budgets for the Mediterranean planktivorous damselfish C. chromis C. chromis. J Exp Mar Biol Ecol 352: Zupo V., Stubing D (21) Diet of fish populations in Posidonia oceanica meadows off the Island of Ischia (Gulf of Naples, Italy): assessment of spatial and seasonal variability. Nat Sci 2: