7.1A Col ection of data for veal calves on farm (measured on farm) Welfare Criteria Measures Good feeding Good housing Good health Quality

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1 7.1A Collection of data for veal calves on farm (measured on farm) Welfare Criteria Measures Good feeding 1 Absence of prolonged hunger Body condition 2 Absence of prolonged thirst Provision of water Good housing 3 Comfort around resting Lying position, cleanliness of calves 4 Thermal comfort Wet calves 5 Ease of movement Slipperiness of the floor Good health 6 Absence of injuries Spots of hard skin, claw lesions, joint lesions, bursae, bitten tail/ear, lameness 7 Absence of disease Coughing, abnormal breathing, nasal discharge, ocular discharge, liquid manure, bloated rumen, dull calves, obviously sick calves, mortality 8 Absence of pain induced by Tail docking management procedures Appropriate behaviour 9 Expression of social behaviours Social behaviours 10 Expression of other behaviours Other behaviours, abnormal behaviours 11 Good human-animal relationship Avoidance distance 12 Positive emotional state Qualitative behaviour assessment 7.1A.1 Good feeding 7.1A.1.1 Absence of prolonged hunger Body condition score according to 7.1A.5 The following criteria are taken into account: Calves should be assessed on the basis of the quantity of muscle, the estimated weight, and physical appearance characteristics including: (i) visibility of the ribs, (ii) the extent to which the backbone protrudes, and (iii) the size of the belly. Calves are compared with the mean level of the batch Calves are compared within the breed/type level within the batch Calves are compared to the mean estimated weight and condition of the rest of the batch within their breed. Animals are scored according to the following indicators (see photopraphic illustration): Normal: the calf is of the same weight and condition as the average of the batch Lower condition: the calf is between 15 and 30% below the average weight or condition of the batch Severe lower condition: the calf is 30% below the average weight or condition of the batch 0 Normal condition 1 Lower condition (approx % less than average) 2 Severe lower condition (approx. 30% less than average) Percentage of lean animals (i.e. score 1) Percentage of very lean animals (i.e. score 2). 114 Welfare Quality Assessment protocol for cattle - 7. applied to veal calves

2 Score 0 Normal condition 7.1A.1.2 Absence of prolonged thirst Score 1 Lower condition (middle ) (approx % less than average) 2006 Van Reenen ASG Score 2 Severe lower condition (left) (approx. 30% less than average) 2006 Van Reenen ASG Provision of water Resource-based measure: Veal calves Animal unit At farm level the availability of water and timing of water supply to calves are assessed. 10 pens are randomly selected in the barn to judge the provision of water. Provision of water is assessed through several indicators: Water supply (assess whether water is provided) Timing Duration when water is available during the day (h) Cleanliness (We consider a water provision point dirty if there is manure on it.) Number of water points per animal Group level: Water supply 0 Water supplied 2 Water not available for animals Timing of water availability 0 Ad libitum access (i.e. more than 12 h a day) 1 Intermediate access (i.e. water is available for 6 to 12 h a day) 2 Restricted access (i.e. water is available for less than 6 h a day) Cleanliness of water points 0 Clean 2 Dirty and Number of water points Welfare Quality Assessment protocol for cattle - 7. applied to veal calves 115

3 7.1A.2 Good housing 7.1A.2.1 Comfort around resting Lying position according to 7.1A.5 Observe the position of calves which are lying. A calf scored as lying with no leg stretched is defined as a calf lying on the sternum with all 4 legs under its body. Observations are performed from the corridor. Per pen 3 bouts of observations of 10 minutes are performed at 3 times of the day (in the morning, at noon and in the afternoon). The observation method used is instantaneous and scan sampling with a sample interval of 2min. For information on the method of observation see 7.1A.5. Group level: Number of scans 1 where calves are lying Number of scans 1 where calves are lying with no leg stretched Percentage of calves 2 lying with no leg stretched out of all calves lying 1 A scan refers to the observation of an individual calf at any given time point. The total number of scans during the behavioural observations is equal to the total number of time points multiplied by the total number of calves observed. 2 Percentage refers to the number of scans where calves lying with no legs stretched is observed expressed as percentage of the total number of scans where calves are observed lying. 7.1A.2.2 Thermal comfort Cleanliness of calves according to 7.1A.5 Individual observation of calves. Calves are assessed for cleanliness of the coat. The coat of calves is observed and scored as clean, moderately dirty or dirty. 0 Clean: no manure 1 Moderately dirty: less then 25% of the surface is covered by manure 2 Dirty: more then 25% of the surface is covered by manure Herd level: Percentage of dirty calves (i.e. score 2) Wet calves according to 7.1A.5 A calf is considered wet when its coat is wet all along its back. (see photographic illustration) 0 Calf not wet 2 Wet calf Percentage of wet calves (i.e. score 2) Not wet Wet 116 Welfare Quality Assessment protocol for cattle - 7. applied to veal calves

4 7.1A.2.3 Ease of movement Slipperiness of the floor Resource-based measure: Veal calves Animal unit Assess 10 pens randomly selected in the barn, in which each pen is given a qualitative slipperiness score based on the assessment of the floor properties by the observer: slightly slippery, moderately slippery, slippery, very slippery, or dangerously slippery. These scales are defined as follows: Not at all slippery Slightly slippery: Slipperiness is limited to a large extent, observed with e.g. dry concrete (slatted) floors, floors covered with a reasonable (e.g. 10 cm) layer of straw Moderately slippery: e.g. dry hard wooden slats with flat surfaces and with (aluminium) antiskid features; wet concrete (slatted) floors Slippery: e.g. hard wooden slats with flat surfaces wet due to urine and faeces and with or without (aluminium) antiskid features Very slippery: It is difficult to stand on the floor, calves cannot move easily and safely. This is observed e.g. on old hard wooden slats with a round surface instead of a flat one, which are wet due to urine and faeces, and with or without antiskid features Dangerous slippery: floors with openings in the floor that are big enough that a leg of calf can fall through, e.g. missing slats Group level: 0 Floor is not slippery 1 Floor is slightly slippery 2 Floor is moderately slippery 3 Floor is slippery 4 Floor is very slippery 5 Floor is dangerously slippery 7.1A.3 Good health 7.1A.3.1 Absence of injuries Spots of hard skin according to 7.1A.5 Spots of hard skin are defined as thickened skin (often wrinkles with loss of hair), often at the shoulders/withers. (see photographic illustration). 0 No evidence of spots of hard skin 2 Evidence of spots of hard skin This measure is combined with all lesions (claw lesions, joint lesions, bursae, spots of hard skin, bitten tail/ear) No spot Spot of hard skin Welfare Quality Assessment protocol for cattle - 7. applied to veal calves 117

5 Claw lesions according to 7.1A.5 Claw lesions are defined as an inflammation with red and swelling skin at the claw border (see photographic illustration). 0 No evidence of claw lesions 2 Evidence of claw lesions This measure is combined with all lesions (claw lesions, joint lesions, bursae, spots of hard skin, bitten tail/ear). No lesion Claw lesion Joint lesions according to 7.1A.5 Joint lesions are defined as clear thickening of the joint border due to accumulation of extra fluids (swelling). (see photographic illustration). 0 No evidence of joint lesions 2 Evidence of joint lesions This measure is combined with all lesions (claw lesions, joint lesions, bursae, spots of hard skin, bitten tail/ear). No lesion Joint lesion 118 Welfare Quality Assessment protocol for cattle - 7. applied to veal calves

6 Bursae according to 7.1A.5 A bursa is defined as a swelling of the front knee (see photographic illustration). 0 No evidence of bursae 2 Evidence of bursae This measure is combined with all lesions (claw lesions, joint lesions, bursae, spots of hard skin, bitten tail/ear). No bursa Bursa Bitten tail/ear according to 7.1A.5 Bitten tail and/or ear is defined as damaged tail/ear with/without presence of fresh blood or scab (see photographic illustration). 0 No evidence of bitten tail and/or ear 1 Evidence of bitten tail/ear without blood and scab 2 Evidence of bitten tail/ear with blood or scab This measure is combined with all lesions (claw lesions, joint lesions, bursae, spots of hard skin, bitten tail/ear). No bitten tail Bitten tail 2006 Van Reenen WUR 2006 Van Reenen WUR Welfare Quality Assessment protocol for cattle - 7. applied to veal calves 119

7 Combined lesions according to 7.1A.5 All lesions are considered altogether according to their severity: Two degrees of severity of external lesions are distinguished: bursa, spot of hard skin, bitten tail/ear without blood or scab are considered mild lesions claw or joint lesion, bitten tail/ear with blood or scab are considered severe lesions 0 No lesion 1 Presence of at least one mild lesion but no severe ones 2 Presence of at least one severe lesion Percentage of animals with no lesion (i.e. score 0) Percentage of animals with mild lesions (i.e. score 1) Percentage of animals with no lesion (i.e. score 2) Lameness according to 7.1A.5 Lameness describes an abnormality of movement and is most evident when the legs are in motion. It is caused by reduced ability to use one or more limbs in a normal manner. Lameness can vary in severity from reduced ability to inability to bear weight. Assess the animal for presence of one of the indicators mentioned below, according to the description for either standing or moving animals. Indicators in moving animals: Reluctance to bear weight on a foot Uneven temporal rhythm between hoof beats, weight not borne for equal time on each of the four feet Indicators in standing animals: Resting a foot (bearing less/no weight on one foot). Frequent weight shifting between feet ( stepping ), or repeated movements of the same foot Standing on the edge of a step 0 No evidence of lameness 2 Evidence of lameness Percentage of animals with lameness (i.e. score 2) 120 Welfare Quality Assessment protocol for cattle - 7. applied to veal calves

8 7.1A.3.2 Absence of disease Coughing according to 7.1A.5 A coughing calf is defined as a calf making audible expulsion of air from the mouth. 0 No evidence of coughing 2 Evidence of coughing Percentage of animals coughing (i.e. score 2) Abnormal breathing according to 7.1A.5 Abnormal breathing rate is defined as a calf performing belly movements, respiratory frequency > 40/min, pumping, excessive nasal movements, and an overall generalised sick appearance. 0 No evidence of abnormal breathing 2 Evidence of abnormal breathing Percentage of animals with abnormal breathing (i.e. score 2) Nasal discharge according to 7.1A.5 Nasal discharge is defined as obvious visible drops from the nostrils; transparent to yellow/green and often with a thick consistency; obvious because the calf does not lick its muzzle (see photographic illustration). 0 No evidence of nasal discharge 2 Evidence of nasal discharge Percentage of animals with nasal discharge (i.e. score 2) No nasal discharge Nasal discharge Welfare Quality Assessment protocol for cattle - 7. applied to veal calves 121

9 Ocular discharge according to 7.1A.5 Ocular discharge is defined as clearly visible flow/discharge (wet or dry) from the eye, at least 3 cm long. 0 No evidence of ocular discharge 2 Evidence of ocular discharge Percentage of animals with ocular discharge (i.e. score 2) Liquid manure (diarrhoea) according to 7.1A.5 In small groups, manure is not observed directly on the calves but on the floor; the absence/presence of liquid manure is recorded pen by pen. In small pens the % of calves affected by liquid manure is assumed to be half of the % pens affected (e.g. if liquid manure is observed in 10% of pens with equal numbers of animals in each pen then the % calves affected is determined to be 5%). In large groups, the presence of liquid manure is observed on all individäl calves (see photographic illustration). Individual level or group level: 0 No evidence of liquid manure 2 Evidence of liquid manure Percentage of animals with liquid manure (i.e. score 2) Normal manure Liquid manure 122 Welfare Quality Assessment protocol for cattle - 7. applied to veal calves

10 Bloated rumen according to 7.1A.5 Bloated rumen is defined as a calf with an obviously tensed belly (top/below/left/ right/both); obviously more convex than the shape of the ribs. (see photographic illustration). 0 No evidence of bloated rumen 2 Evidence of bloated rumen Percentage of animals with bloated rumen (i.e. score 2) Normal Dull calf according to 7.1A.5 A dull calf is defined as a calf with the entire coat being dull, not shiny or glossy, sometimes with the coat additionally appearing as dry and/or long (see photographic illustration). 0 Calf not dull 2 Dull calf Percentage of dull calves (i.e. score 2) Bloated Rumen Normal coat Dull coat Welfare Quality Assessment protocol for cattle - 7. applied to veal calves 123

11 Obviously sick calf according to 7.1A.5 Assess for presence of obviously sick calves. An obviously sick calf is defined as an apathetic calf and/or an animal showing hanging ears and/or a body position indicating pain (e.g. rounded back). (see photographic illustration). 0 Calf not obviously sick 2 Obviously sick calf Percentage of obviously sick calves (i.e. score 2) Not obviously sick Obviously sick Mortality Management-based measure: Veal calves Animal unit Mortality is defined as the uncontrolled death of animals as well as cases of euthanasia and emergency slaughter. The animal unit manager is asked about the number of animals which died on the farm, were euthanized due to disease or accidents or were emergency slaughtered during the last 12 months. Additionally the average number of animals with a weight of more than 200 kg live weight in the animal unit is asked. Farm records may also be used. Percentage of animals dead on the farm during the last 12 months (beginning or end of fattening period). Optional additional information 124 Welfare Quality Assessment protocol for cattle - 7. applied to veal calves

12 7.1A.3.3 Absence of pain induced by management procedures 7.1A.4 Appropriate behaviour Tail docking Management-based measure: Veal calves Animal unit The animal unit manager is asked about tail docking practices on the farm with regard to the following items: Procedures for tail docking Use of anaesthetics Use of analgesics Herd level: 0 No tail docking 1 Tail docking using rubber rings 2 Tail docking using surgery and 0 Use of anaesthetics 2 No use of anaesthetics and 0 Use of analgesics 2 No use of analgesics 7.1A.4.1 Expression of social behaviours Social behaviours according to 7.1A.5 Assess the veal calves for presence of the two social behaviours mentioned below: Social licking is defined as licking, nibbling and sniffing a pen mate at the part of the body where the hair is long: head, shoulders, flanks, back and tail but not the lower parts (legs, under the belly). Head bumping is defined as a calf that has a head to head fight or is horning or displaces another calf. Observations are performed from the corridor, in bouts of 10 minutes per pen at 3 times of the day (in the morning, at noon and in the afternoon). The observation method used is instantaneous scan sampling with a sample interval of 2min (5 scans per 10 min). For information on the method of observation see 7.1A.5. Group level: Number of scans 1 when social licking is observed Number of scans when head bumping is observed Total number of scans made Percentage of scans when social licking is observed out of the total number of scans made Percentage of scans when head bumping is observed out of the total number of scans made 1 A scan refers to the observation of an individual calf at any given time point. The total number of scans during the behavioural observations is equal to the total number of time points multiplied by the total number of calves observed. Welfare Quality Assessment protocol for cattle - 7. applied to veal calves 125

13 7.1A.4.2 Expression of other behaviours Play behaviours according to 7.1A.5 Assess the veal calves for the occurrence of running, jumping and frolic behaviour: Behaviour Running/Jumping/Frolic behaviour Description Calf runs and/or jumps with 2 or more legs off the ground. Observations are performed from the corridor. 3 bouts of 10 minutes per pen of observations are performed at 3 times of the day (in the morning, at noon and in the afternoon). The observation method used is instantaneous scan sampling with a sample interval of 2 min (5 scans per 10 min). For information on the method of observation see 7.1A.5. Group level: Number of scans 1 when running/jumping/frolic behaviour is observed Number of scans when running/jumping/frolic behaviour is observed Total number of scans where calves were not lying Percentage of scans where running/jumping/other frolic behaviour was observed out of total number of scans where calves were not lying 1 A scan refers to the observation of an individual calf at any given time point. Abnormal oral behaviours according to 7.1A.5 Assess the veal calves for the occurrence of the following abnormal behaviours: Behaviour Tongue rolling Manipulating substrates Cross-sucking Description Calf makes repeated movements with its tongue in or outside its mouth Calf licks, sucks or bites at the fence, wall, bucket, trough, floor or any other non-food and inanimate object. Calf drinks or licks the urine of a penmate, or calf sucks or licks the prepuce of a penmate. Observations are performed from the corridor. 3 bouts of 10 minutes per pen of observations are performed at 3 times of the day (in the morning, at noon and in the afternoon). The observation method used is instantaneous scan sampling with a sample interval of 2min (5 scans per 10 min). For information on the method of observation see 7.1A.5. Group level: Number of scans 1 when abnormal oral behaviour is observed Number of scans when abnormal oral behaviour is observed Total number of scans made Percentage of scans 1 where abnormal oral behaviour was observed out of scans where calves were not lying 1 A scan refers to the observation of an individual calf at any given time point. 126 Welfare Quality Assessment protocol for cattle - 7. applied to veal calves

14 7.1A.4.3 Good human animal relationship Avoidance distance/calf escape test according to 7.1A.5 The assessor enters the pen, or stays in the pen if the calves have to be woken up, and waits 1 minute to let the calves get used to the presence of the assessor. The assessor chooses a calf (in small groups: any calf / in large groups: one of the calves on the list) which stands in front of the assessor at a distance of about two steps. The assessor tries to make eye contact. The assessor slowly (1 step per second) takes 1 step toward the calf (with a stretched arm) and stands still with two feet next to each other for 1 second. He notes whether the calf is standing still or not (i.e. forelegs moved or not). The assessor takes the second step (again 1 step per second) and stands still with two feet next to each other for 1 second. Again the assessor notes whether the calf stands still or not. If the calf stands still the assessor tries to touch the calf on the muzzle (or the calf approaches the assessor and makes contact). The maximum time to try this is 5 seconds. The assessor notes if contact was successful or not. Parameters: Note the ear tag number of the calf Give a score to the calf using the following grid: Score a b c d e Description no eye contact eye contact succeeded, step 1 results in a withdrawal step 1 succeeded, step 2 results in a withdrawal step 2 succeeded, calf can not be touched calf can be touched Up to 3 attempts are made per calf to make eye contact successfully. If the assessor did not achieve eye contact (active avoiding by the calf) after these 3 chances, this will be noted. In large groups, if it is not possible to approach the calf (the calf remains in the opposite part of the pen, hides behind other calves) the calf will be recorded as no eye contact (score a). Ethogram: Standing still is defined as the calf remaining oriented toward the human after the human has made one step or has touched the calf (i.e. it did not move its forelegs). Escape behaviour is defined as when the calf makes a move to move away from the human by moving backward or turning around (i.e. no longer oriented towards the human). In small groups all calves in a pen are tested, in large groups a random selection of the calves in a pen is tested, depending on the number of pens at a farm. Percentage of calves which allow the assessor to approach for two steps as described in the protocol 7.1A.4.4 Absence of fearfulness Qualitative Behaviour Assessment Animal unit (depending on number of observation points, see method description) Qualitative Behaviour Assessment (QBA) considers the expressive quality of how animals behave and interact with each other and the environment, i.e. their body language. Welfare Quality Assessment protocol for cattle - 7. applied to veal calves 127

15 Select between one and eight observation points (depending on the size and structure of the farm) that together cover the different areas of the farm. Decide the order to visit these observation points, wait a few minutes to allow the animals to return to undisturbed behaviour. Watch the animals that can be seen well from that point and observe the expressive quality of their activity at group level. It is likely that the animals will initially be disturbed, but their response to this can be included in the assessment. Total observation time should not exceed 20 minutes, and so the time taken at each observation point depends on the number of points selected for a farm: Number of observation points Duration of observation per observation point in minutes When observation at all selected points has been completed, find a quiet spot and score the 20 descriptors using the visual analogue scale (VAS). Please note that scoring is not done during observation, and that only one integrative assessment is made per farm. Each VAS is defined by its left minimum and right maximum point. Minimum means that at this point, the expressive quality indicated by the term is entirely absent in all of the animals you have seen. Maximum means that at this point this expressive quality is dominant across all observed animals. Note that it is possible to give more than one term a maximum score; animals could for example be both entirely calm and entirely content. To score each term, draw a line across the 125 mm scale at the appropriate point. The measure for that term is the distance in millimetres from the minimum point to the point where the line crosses the scale. Do not skip any term. Please be aware when scoring terms that start with a negative pre-fix, such as unsure or uncomfortable. As the score gets higher, the meaning of the score gets more negative, not more positive. The terms used for veal calves QBA assessment are: Active Indifferent Boisterous Relaxed Frustrated Uneasy Fearful Friendly Sociable Agitated Bored Apathetic Depressed Playful Happy Calm Lively Distressed Tense Inquisitive Herd level: Continuous scales for all body language parameters from minimum to maximum 7.1A.5 Sampling and practical information Organization of the visit The visit takes place at weeks after arrival of the calves in the veal unit. The assessor arrives in the veal unit, takes 5 minutes to become familiar with the barn and checks with the animal unit manager the number of calves in the sickbay or separated from the batch. The assessor takes then 5 minutes to become familiar with the mean weight and condition of calves of the batch (for the criteria body condition score). He then performs the different observations on clinical health and behaviour of the calves (see Table 21). 128 Welfare Quality Assessment protocol for cattle - 7. applied to veal calves

16 At the end of the observation, the assessor fills in a short questionnaire with the animal unit manager asking about on-going collective treatments, the health status of the batch on arrival, the appetite of the animals on arrival and on the week of the visit, the mortality since the last visit and the causes of mortality. Parameter Time needed approximately 1 Qualitative behaviour assessment (QBA) All calves in the batch min 2 Resource checklist All pens 30 min Number of calves in the pen Provision of water 10 pens Slipperiness of floor 10 pens 3 Clinical measures 200 to 300 animals: 120 min Abnormal breathing Nasal discharge If size of the batch <200: all animals Ocular discharge Liquid manure Bloated rumen If size of the batch >200: 300 animals Dull calf Wet calves Claw lesions Joint lesions Bursae Bitten tail/ear Spots of hard skin Body condition score Coughing calves Obviously sick calves Cleanliness of calves 4 Scan sampling In large groups: 3 x 1-3 pens 3 x 30 min Social behaviour In small groups: 3 x 12 pens Other behaviour Abnormal behaviour Lying positions 5 Avoidance distance/calf escape test 100 animals selected randomly 60 min 6 Management questionnaire Animal unit (interview with animal unit manager) 15 min Tail docking Mortality TOTAL min hours Table 21 Order for carrying out measures, sample size and time required for veal calves on farm. Selecting veal calves for clinical assessment Sampling method Visual observation of each individual calf takes place in the corridor (for calves housed in small groups) or in the home pen (for calves housed in large groups) for the measures body condition score, wet calves, claw lesions, joint lesions, bursae, lameness, spots of hard skin, bitten tail/ear, all respiratory disorders, all enteric disorders, dull calves and obviously sick calves and dirty calves. Furthermore, for all clinical health measures sample sizes are defined as follows: In small groups: When size of the batch = 200 calves, all calves are observed (all pens are observed) When size of the batch is > 200 calves a maximum of 300 calves are observed. Up to 60 pens (depending on number of animals per pen) are randomly chosen using their numbers in a list). Welfare Quality Assessment protocol for cattle - 7. applied to veal calves 129

17 Remark: the lsize limit of the batch, as when all or a part of the calves on the unit have to be observed, could be extended to 300. Observations are performed at the pen level. The assessor stands in the corridor in front of the pen. The assessor takes 3 minutes to observe each pen and to record all of the information at individual and pen level. At the end of the observation the assessor moves to the next pen until the full sample is observed. In large groups: 100 calves are observed. They are randomly chosen in the batch and equally distributed amongst pens Observation is performed at the individual level. The assessor enters the pen and randomly chooses a calf. He takes 1 minute to record all the information. At the end of the observation he chooses another calf until 100 calves are observed (the number of calves observed should be equally distributed amongst pens) Selecting veal calves for behaviour assessment Scan sampling observations Sampling method: For these measures sample sizes are the following: In small groups 36 pens (or if less pens available, all pens) are observed. In large groups a maximum of 9 pens is observed (between 20 and 100 calves per pen). Preparation: Ensure that the lights are on. In small groups, 4 pens are observed at the same time. In large groups, 1 pen is observed. Observations take 10 minutes with 5 scans at 2 min intervals. Pens which are observed and the order in which they are observed are previously randomly selected. 10 min observations are performed three times with different pens (i.e. 3x 4 pens) for small groups. For large groups it depends on the number of pens on the farm. If possible repeat observations three times with different pens (i.e. 3 x 1 pen). Observation: The assessor takes place in the middle of the 4 pens, or at the front of the pen, with large groups. The assessor waits five minutes before starting the observation, so that the calves can get used to him/her. After these 5 minutes, the stopwatch is started and the observation starts with a scan of the first pen, further observations are made in a clockwise direction around the pens. Every 2 minutes the behaviour of all calves is scan sampled using the ethogram. The total number of observations per pen is 5 scans within 10 minutes. Repetition At the end of the 10 min observation period the assessor walks to the next four pens (in small pen group systems) or to the next large pen (in large pen group systems) and the procedure starts again. Observations as outlined above take place three times during the day (morning, noon, afternoon) using different pens if available. Avoidance distance / Calf escape test Preparation: Pens are randomly selected and so the test order is defined. Small groups: all calves in a pen will be observed Large groups: a percentage of the calves (randomly selected before the test; using a list of ear tag numbers) will be observed Ensure that the lights are on. In small groups, all the calves have to get up, if necessary woken up by the assessor. For large groups it is not necessary to rouse all calves at the beginning of the test. Calves will be tested when they are standing. The approach of each calf is focussed on the head, i.e. the assessor tries to touch the snout /muzzle with an arm that is stretched out a little. 130 Welfare Quality Assessment protocol for cattle - 7. applied to veal calves

18 Qualitative behaviour assessment (QBA) The assessor should first familiarize him/herself with the unit, decide on the observation point/s, fills out the form (name, date, time of the day, farm, housing unit, number of animals in unit, breed) and describe the system. The assessor then leaves the unit for 5-10 minutes in order to let the animals go back to their normal undisturbed behaviours. The assessor enters the unit and observes how the animals behave for 10 to 2.5 minutes at each observation point (10 min if only one area is observed). After all the observation points have been visited, the assessor walks out of the unit and scores the QBA descriptors on a scale from 0 to 125 mm. 7.1B Collection of data for veal calves on farm welfare (measured at slaughterhouse) These measures are assessments of disease which are made at the slaughterhouse but they reflect disease conditions indicating the farm life of the veal calves and are not reflections of the slaughter process. Therefore they are calculated together with the previous on-farm assessment, and jointly form the basis for the overall assessment for veal calves on farm. Welfare Criteria Measures Good feeding 1 Absence of prolonged hunger This criterion is not applied in this situation 2 Absence of prolonged thirst This criterion is not applied in this situation Good housing 3 Comfort around resting This criterion is not applied in this situation 4 Thermal comfort This criterion is not applied in this situation 5 Ease of movement This criterion is not applied in this situation Good health 6 Absence of injuries This criterion is not applied in this situation 7 Absence of disease Lung lesions, abomasal lesions, rumenal plaque 8 Absence of pain induced by This criterion is not applied in management procedures this situation Appropriate behaviour 9 Expression of social behaviours This criterion is not applied in this situation 10 Expression of other behaviours This criterion is not applied in this situation 11 Good human-animal relationship This criterion is not applied in this situation 12 Absence of fearfulness This criterion is not applied in this situation 7.1B.1 Good feeding 7.1B.1.1 Absence of prolonged hunger This criterion is not applied in this situation. 7.1B.1.2 Absence of prolonged thirst This criterion is not applied in this situation. Welfare Quality Assessment protocol for cattle - 7. applied to veal calves 131

19 7.1B.2 Good housing 7.1B.2.1 Comfort around resting This criterion is not applied in this situation. 7.1B.2.2 Thermal comfort This criterion is not applied in this situation. 7.1B.2.3 Ease of movement This criterion is not applied in this situation. 7.1B.3 Good health 7.1B.3.1 Absence of injuries This criterion is not applied in this situation. 7.1B.3.2 Absence of disease Lung lesions according to 7.1B.5 For this measure 100 to 200 lungs per batch are assessed for the presence of lung lesions. Visual observations take place for each individual lung on the slaughter line. The assessor identifies the beginning of the batch of calves to observe. He stands just before the meat inspection control were the lungs are detached from the carcass and put onto the line. He observes the first 100 or 200 lungs from the batch or all lungs if the batch is less than 100 calves. He performs an inspection of the right and left sides of the lung (5 cranial lobes) spending about 10 seconds per lung. Each lung is given a score for pneumonia discoloration (0 to 3) and for attached tissues due to pleuritis (absent or present). For pneumonia discoloration, the left and right side of the lungs (cranial lobes) are observed and scored using the scales below: Individual level Discoloration Description 0 No Normal pale orange colour 1 Minimal One spot of grey-red discoloration 2 Mild/moderate One larger or several small spots of grey-red discoloration (less than one lobe) 3 severe Grey-red discoloration area (at least one full lobe) and/or presence of abscesses The worst score obtained by one of the two sides of the lung is attributed to the lung (see photographic illustration). For attachment ot tissues (pleuritis), the whole lung is observed and scored using the following grid: 132 Welfare Quality Assessment protocol for cattle - 7. applied to veal calves Individual level Attached tissues Description 0 No All lobes can be separated 2 Yes Lobes are attached together or to other tissues (e.g. pericardium)

20 Lung lesion Score 0: no pneumonia Normal pale orange colour Lung attached tissues: The percentage of lungs with attached tissues and the mean discoloration score and percentage of lungs for each score of discoloration of the batch are calculated. See photographic illustration. Herd level: Percentage of lungs for each score of discoloration and Percentage of lungs with attached tissues (i.e. score 2) Score 1 : minimal pneumonia 1 spot of grey-red discoloration Score 2 : mild/moderate pneumonia 1 larger or several small spots of grey-red discoloration (less than 1 lobe) Score 3 : severe pneumonia Grey-red discoloration area (at least one full lobe) and/or presence of abscesses Score 0: absent All lobes can be separated Score 2 : present Lobes are attached together or to other tissues (e.g. pericardium) Welfare Quality Assessment protocol for cattle - 7. applied to veal calves 133

21 Number 0 1 Abomasal lesions according to 7.1B.5 For this measure 60 abomasums per batch are assessed for the presence of abomasal lesions. Visual observation of each abomasum is performed to evaluate the incidence of mucosal lesions (see photographic illustration). The assessor identifies the beginning of the batch of calves to observe, while standing in the room where the bowels are cleaned. For the 60 abomasum observed, a cm section of the pyloric area is cut and placed in a separate box. These sections are longitudinally cut and cleaned with water by the assessor. He observes the sections and counts the number of lesions (from erosions to open ulcers) using the following grid: Size of lesion <0,5cm 2 0,5 to 1cm 2 >1 cm 2 count 0, 1, 2, 3, 4 and + 0, 1, 2, 3, 4 and + 0, 1, 2, 3, 4 and + After observation, the abomasal sections are put into the animal products bin with the rest of the waste. Mean lesion score: Mean lesion score (0 to 24)= [(no. of lesions size 1 x 1) + (no. of lesions size 2 x 2) + (no. of lesions size 3 x 3)] The % of abomasums with at least one large lesion (size 3) and the mean lesion score are calculated for each batch. Size 1 : < 0,5 cm 2 2 : 0,5 to 1 cm 2 3 : > 1 cm 2 2 and Welfare Quality Assessment protocol for cattle - 7. applied to veal calves

22 Rumenal plaque according to 7.1B.5 For this measure 60 rumens per batch are assessed for presence of plaque. Plaque is a pathological condition of the rumenal wall, consisting of multiple patches on the rumen mucosa with coalescing and adhering papillae covered by a sticky mass of feed, hair and cell debris (which feel like spots of chewing gum trampled into a door mat). (see photographic illustration). Record whether or not there was any plaque present. 0 Rumenal plaque not present 2 Rumenal plaque present Herd level: The percentage of rumens with at least one plaque. Score 0: absent 7.1B.3.3 Absence of pain induced by management procedures This criterion is not applied in this situation. 7.1B.4 Appropriate behaviour 7.1B.4.1 Expression of social behaviours This criterion is not applied in this situation. 7.1B.4.2 Expression of other behaviours This criterion is not applied in this situation. 7.1B.4.3 Good human animal relationship This criterion is not applied in this situation. Score 2: present 7.1B.4.4 Absence of fearfulness This criterion is not applied in this situation. Welfare Quality Assessment protocol for cattle - 7. applied to veal calves 135

23 7.1B.5 Sampling and practical information Sampling Observations are performed at commercial slaughter houses where the observed batches are slaughtered. Batches can be slaughtered in one group or on several days. In the second case, one or two dates are chosen for observations depending on the size of the groups of calves slaughtered. For each batch 100 or 200 lungs, 60 abomasum and 60 rumens are observed. Furthermore, see Table 22. Parameter Time needed approximately Clinical measures Lung lesions 100 to 200 lungs Min 60 min Abomasal lesions 60 abomasums Min 120 min Rumenal plaque 60 rumens Table 22 and time required for measures for veal calves (on farm) collected at the slaughterhouse. 7.2 Calculation of scores for veal calves on farm Criterion-scores Absence of prolonged hunger The % of lean calves and the % very lean calves are used to calculate an index: index = (w 1 (%lean) + w 2 (%lean))/w 2 with w 1 = 0.35 and w 2 = 1 Then the index is transformed into a score using I-spline functions (Figure 29): When I 75 then Score = ( x I) + ( x I 2 ) + ( x I 3 ) When I 75 then Score = ( x I) ( x I 2 ) + ( xi 3 ) Score % lean calves (weighted for severity) Figure 29 Calculation of scores for absence of hunger according to % lean animals (Weight: 0.35 for lean calves and 1 for very lean calves). 136 Welfare Quality Assessment protocol for cattle - 7. applied to veal calves