CERTIFICATE OF ANALYSIS - GC PROFILING
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1 Date : March 07, 2018 CERTIFICATE OF ANALYSIS - GC PROFILING SAMPLE IDENTIFICATION Internal code : 18B20-PLG29-1-CC Customer identification : Allspice Type : Essential oil Source : Pimenta officinalis Customer : Plant Guru ANALYSIS Method: PC-PA J19 - Analysis of the composition of an essential oil, or other volatile liquid, by FAST GC-FID (in French); identifications validated by GC-MS. Analyst : Sarah-Eve Tremblay, M. Sc. A., Chimiste Analysis date : March 06, 2018 Checked and approved by : Alexis St-Gelais, M. Sc., chimiste Note: This report may not be published, including online, without the written consent from Laboratoire PhytoChemia. This report is digitally signed, it is only considered valid if the digital signature is intact. Page 1/9
2 PHYSICOCHEMICAL DATA Physical aspect: Light yellow liquid Refractive index: ± (20 C) CONCLUSION No adulterant, contaminant or diluent has been detected using this method. Page 2/9
3 ANALYSIS SUMMARY Identification DB-5 (%) DB-WAX (%) Classe Toluene tr tr Simple phenolic (3Z)-Hexenol tr tr Aliphatic alcohol α-thujene Monoterpene α-pinene Monoterpene β-pinene tr 0.01 Monoterpene Myrcene Monoterpene α-phellandrene Monoterpene Δ3-Carene Monoterpene α-terpinene Monoterpene para-cymene Monoterpene Limonene 0.21* 0.06 Monoterpene 1,8-Cineole [0.21]* 0.16* Monoterpenic ether β-phellandrene [0.21]* [0.16]* Monoterpene (Z)-β-Ocimene Monoterpene (E)-β-Ocimene Monoterpene γ-terpinene Monoterpene Terpinolene Monoterpene trans-linalool oxide (fur.) Monoterpenic alcohol Linalool Monoterpenic alcohol Terpinen-4-ol * Monoterpenic alcohol para-cymen-8-ol Monoterpenic alcohol α-terpineol Monoterpenic alcohol Methylchavicol * Phenylpropanoid Chavicol Phenylpropanoid Eugenol Phenylpropanoid α-copaene Sesquiterpene β-elemene * Sesquiterpene α-gurjunene Sesquiterpene Methyleugenol Phenylpropanoid β-caryophyllene 5.83 [5.99]* Sesquiterpene β-copaene Sesquiterpene Aromadendrene 0.06 [0.16]* Sesquiterpene trans-α-bergamotene 0.02 [5.99]* Sesquiterpene α-humulene 1.04 [1.03]* Sesquiterpene allo-aromadendrene Sesquiterpene Selina-4,11-diene Sesquiterpene γ-muurolene * Sesquiterpene α-amorphene 0.03 [0.08]* Sesquiterpene β-selinene Sesquiterpene α-selinene 0.61* 0.47 Sesquiterpene Viridiflorene [0.61]* 0.15 Sesquiterpene α-muurolene Sesquiterpene γ-cadinene * Sesquiterpene trans-calamenene 0.31* 0.03 Sesquiterpene δ-cadinene [0.31]* [0.32]* Sesquiterpene Zonarene [0.31]* [0.32]* Sesquiterpene trans-cadina-1,4-diene Sesquiterpene α-cadinene Sesquiterpene Page 3/9
4 α-calacorene Sesquiterpene Caryophyllene oxide isomer 0.18* 0.03 Sesquiterpenic ether Spathulenol [0.18]* 0.01 Sesquiterpenic alcohol Caryophyllene oxide [0.18]* 0.16 Sesquiterpenic ether Globulol Sesquiterpenic alcohol Viridiflorol Sesquiterpenic alcohol Cubeban-11-ol Sesquiterpenic alcohol Eudesm-5-en-11-ol * Sesquiterpenic alcohol Methoxyeugenol Phenylpropanoid Unknown Oxygenated sesquiterpene Rosifoliol 0.04 [0.04]* Sesquiterpenic alcohol τ-muurolol 0.07* 0.06 Sesquiterpenic alcohol τ-cadinol [0.07]* [82.55] Sesquiterpenic alcohol α-cadinol 0.22* 0.07 Sesquiterpenic alcohol Selin-11-en-4α-ol [0.22]* 0.15 Sesquiterpenic alcohol Total identified 98.72% 98.82% *: Two or more compounds are coeluting on this column [xx]: Duplicate percentage due to coelutions, not taken account in the identified total tr: The compound has been detected below 0.005% of total signal. Note: no correction factor was applied Page 4/9
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8 FULL ANALYSIS DATA Identification Column DB-5 Column DB-WAX R.T R.I % R.T R.I % Toluene tr tr (3Z)-Hexenol tr tr α-thujene α-pinene β-pinene tr Myrcene α-phellandrene Δ3-Carene α-terpinene para-cymene Limonene 4.48* ,8-Cineole 4.48* 1022 [0.21] 3.49* β-phellandrene 4.48* 1022 [0.21] 3.49* 1167 [0.16] (Z)-β-Ocimene (E)-β-Ocimene γ-terpinene Terpinolene trans-linalool oxide (fur.) Linalool Terpinen-4-ol * para-cymen-8-ol α-terpineol Methylchavicol * Chavicol Eugenol α-copaene β-elemene * α-gurjunene Methyleugenol β-caryophyllene * 1547 [5.99] β-copaene Aromadendrene * 1555 [0.16] trans-α- Bergamotene * 1547 [5.99] α-humulene * 1614 [1.03] allo- Aromadendrene Selina-4,11- diene γ-muurolene * α-amorphene * 1638 [0.08] β-selinene α-selinene 11.34* Viridiflorene 11.34* 1484 [0.61] α-muurolene γ-cadinene * trans * Page 8/9
9 11-ol Methoxyeugenol Unknown [m/z 43, 81 (97), 135 (71), 95 (62), 204 (61), 71 (59), 207 (56) (3)] * 1642 [0.22] Calamenene δ-cadinene 11.74* 1514 [0.31] 10.68* 1706 [0.32] Zonarene 11.74* 1514 [0.31] 10.68* 1706 [0.32] trans-cadina-1,4- diene α-cadinene α-calacorene Caryophyllene oxide isomer 12.44* Spathulenol 12.44* 1568 [0.18] Caryophyllene oxide 12.44* 1568 [0.18] Globulol Viridiflorol Cubeban-11-ol Eudesm-5-en * Rosifoliol * 2055 [0.04] τ-muurolol 13.22* τ-cadinol 13.22* 1632 [0.07] [82.55] α-cadinol 13.36* Selin-11-en-4αol Total identified 98.72% 98.82% Total reported 98.80% 98.89% *: Two or more compounds are coeluting on this column [xx]: Duplicate percentage due to coelutions, not taken account in the identified total : Peaks apexes were resolved, but peaks overlapped and were summed for analysis tr: The compound has been detected below 0.005% of total signal. Note: no correction factor was applied R.T.: Retention time (minutes) R.I.: Retention index Page 9/9
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