CONSTANT PRESSURE SYRINGE PUMP

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1 A NEW TECHNOLOGY BREAKTHROUGH CONSTANT PRESSURE SYRINGE PUMP ENABLES CONTINUOUS PRESSURE-CONTROLLED INFUSION USE IN CONSTANT FLOW OR CONSTANT PRESSURE MODE ENHANCES SAFETY FOR SENSITIVE EXPERIMENTATION COMPATIBLE WITH A WIDE RANGE OF PRESSURE TRANSDUCERS AUTOMATICALLY ADJUSTS FLOW RATE TO MAINTAIN CONSTANT PRESSURE F O R YO U R F LU I D I C ULTIMATE PUMP A P P L I C A T I O N

INTRODUCTION PHDULTRA CP Constant Pressure Syringe Pump Technology Breakthrough!

This pump is the first of its kind to allow operation under constant pressure or constant flow.

output, results in a constant pressure dispensing system.

Our user-friendly touch screen interface allows for simple control of either constant pressure or constant flow-based

88-3016 4400 Infusion/Withdrawal Programmable 88-3015 Infusion/Withdrawal Programmable Superior Functionality Using Harvard

2 INTRODUCTION PHDULTRA CP Constant Pressure Syringe Pump Technology Breakthrough! Harvard Apparatus is proud to introduce the syringe pump. This pump is the first of its kind to allow operation under constant pressure or constant flow. Historically, the only means available to dispense at constant pressure has involved the use of various amplifiers and other accessories/ software. The, when combined with virtually any commercially available pressure transducer/amplifier combination with 0-10V DC analog output, results in a constant pressure dispensing system. This sytem can deliver fluids with an applied force up to 1,000 lbs. Our user-friendly touch screen interface allows for simple control of either constant pressure or constant flow-based applications Infusion/Withdrawal Programmable Infusion/Withdrawal Programmable Superior Functionality Using Harvard Apparatus syringe pump technology and software-controlled pressure monitoring, the is able to maintain a user-defined system pressure ±2%. A user-adjustable sensitivity setting allows for the customization of the system response time necessary to attain the set pressure. The allows the user to set pressures in the units of their choice including mmhg, kpa, and psi. Constant Pressure Applications Include: Short-term organ perfusion studies that require the maintenance of physiologic conditions. Introduction of chemical reactants in a controlled manner. Ocular injections and perfusions. Small animal whole-body perfusions. Constant pressure microfluidic mixing. Administration of genetic material into organs without viral vectors. And Many More! To order call techsupport@harvardapparatus.com

3 SYRINGE PUMP SOFTWARE PRECISION MONITORING OF PRESSURE & FLOW Harvard Apparatus Constant Pressure Monitoring Software. Pressure & Flow Rate Data Data can be monitored via RS-232 from the to a PC In practice, the 0-10V analog output of a pressure transducer amplifier is connected to the analog input on the rear panel of the pump. The amplifier or signal conditioner can be provided by the customer, or accomplished with various tranducers and amplifiers available from Harvard Apparatus. The pressure range may be scaled to fit the available transducer voltage output for systems that output less than 10 V. While in constant pressure mode, in addition to the set and actual pressure, the pump displays the flow rate. This data may be output for further analysis with a variety of data acquisition packages. Accuracy & Reproducibility Of course, the may be used in flow mode with its worldrenowned accuracy and reproducibility. All pumps are infuse/withdraw models. When used in flow mode, these offer features such as method storage and flow programming functions to allow the user to create simple to complex methods. The is available in a variety of configurations to suit the desired pressure or flow rate ranges. To order call techsupport@harvardapparatus.com

ACCESSORIES SUPERIOR SELECTION OF ACCESSORIES Essential Application Tools The can work with any pressure transducer and amplifier that will output a 0-10V or similar signal.

Our most popular physiological pressure transducer is our APT-300, a fluid-filled unit with a pressure range of ±300mmHg and a sensitivity of 5µV/V/mmHg (±1%).

This pressure transducer has a pressure range of ±75mmHg and a sensitivity of 1mV/mmHg.

As a PLUGSYS module, this DC Bridge Amplifier requires the use of a PLUGSYS housing with a power supply. Cases holding 2, 5, or 10 modules are available.

compatible. Millar Transducers interface directly with the TAM-A amplifier.

These include Harvard Apparatus Stainless Steel syringes for high pressure applications as well as Hamilton microliter and Gastight syringes in a variety of sizes.

4 ACCESSORIES SUPERIOR SELECTION OF ACCESSORIES Essential Application Tools The can work with any pressure transducer and amplifier that will output a 0-10V or similar signal. Harvard Apparatus carries a wide variety of pressure transducers and amplifiers to suit your particular application. Our most popular physiological pressure transducer is our APT-300, a fluid-filled unit with a pressure range of ±300mmHg and a sensitivity of 5µV/V/mmHg (±1%). This has an easily replaceable transducer head. For low pressure applications, such as maintaining the pressure in an eye, we have the P75 Venous Pressure Transducer. This pressure transducer has a pressure range of ±75mmHg and a sensitivity of 1mV/mmHg. When the customer requires a separate amplifier, the unit of choice is the model 601 Transducer Amplifier Module, or TAM-A. As a PLUGSYS module, this DC Bridge Amplifier requires the use of a PLUGSYS housing with a power supply. Cases holding 2, 5, or 10 modules are available. APT-300 with Minicase Blood Pressure Transducer P75 Samba Sensor Applications requiring the use of solid-state pressure catheters (as small as 1Fr up to 7Fr) are also compatible. Millar Transducers interface directly with the TAM-A amplifier. Harvard Apparatus provides a full line of accessories to complement any fluidics experiment, as well as syringes in a variety of styles and sizes. These include Harvard Apparatus Stainless Steel syringes for high pressure applications as well as Hamilton microliter and Gastight syringes in a variety of sizes. Tubing choice is essential to any fluidics set-up. The length, type and stiffness of your tubing will affect the flow quality and accuracy of any experiment. Harvard Apparatus provides a full line of Tygon tubing, PEEK tubing and even can provide custom lengths of stainless steel tubing. Please contact us for more information. 200 ml 100 ml Harvard Apparatus High Pressure Stainless Steel Syringes Research Grade Blood Pressure Transducer 50 ml 2.5 ml 20 ml 8 ml To order call techsupport@harvardapparatus.com

SPECIFICATIONS & ORDERING INFORMATION FAMILY OF SYRINGE PUMPS Model PHD ULTRA CP Remote 4400 4400 Remote HPSI Remote PHD ULTRA XF-CP Remote Type single syringe,

5 µl to 140 ml 0.5 µl to 140 ml 0.5 µl to 140 ml 0.5 µl to 140 ml 20 ml to 200 ml 20 ml to 200 ml # Syringes 2-10 2-10 1 1 4 4 Flow Rate: 88-3316 4400 Remote 88-3113 HPSI Remote Minimum 1.

1 ml/min Connectors: USB (PC-to-Pump) Type B Type B Type B Type B Type B Type B RS-232 (PC-to-Pump) 9-Pin D-Sub 9-Pin D-Sub 9-Pin D-Sub 9-Pin D-Sub 9-Pin D-Sub

15-Pin D-Sub 15-Pin D-Sub 15-Pin D-Sub 15-Pin D-Sub 15-Pin D-Sub Average Linear Force 34 kg (75 lbs) 34 kg (75 lbs) 91 kg (200 lbs) 91 kg (200 lbs) 200 kg (433

6 cm 30.5 x 21.6 x 10.6 cm 30.5 x 21.6 x 18.5 cm 30.5 x 21.6 x 10.6 cm 30.5 x 21.6 x 10.6 cm Control Box (12 x 8.5 x 7 in) (12 x 8.5 x 4.3 in) (12 x 8.5 x 4.3 in) (12 x 8.5 x 7.3 in) (12 x 8.5 x 4.3 in) (12 x 8.5 x 4.3 in) Dimensions 30.

5 x 12 x 7.5 in) Weight 4.5 kg (10 lb) 4.8 kg (10.5 lb) 5.4 kg (12 lb) 7.7 kg (17 lb) 13.6 kg (30 lb) 20.

VAC; 50/60 Hz 100-240 VAC; 50/60 Hz Order # 88-3015 88-3315 88-3016 88-3316 88-3113 88-3314 * Option for RS-232 (RJ-11, Pump-to-Pump) must be ordered at time the

5 SPECIFICATIONS & ORDERING INFORMATION FAMILY OF SYRINGE PUMPS Model PHD ULTRA CP Remote Remote HPSI Remote PHD ULTRA XF-CP Remote Type single syringe, single syringe, Flow Accuracy ±0.25% ±0.25% ±0.35% ±0.35% ±0.5% ±0.5% Pressure Accuracy < ±2% < ±2% < ±2% < ±2% < ±2% < ±2% Syringe Size, min/max 0.5 µl to 140 ml 0.5 µl to 140 ml 0.5 µl to 140 ml 0.5 µl to 140 ml 20 ml to 200 ml 20 ml to 200 ml # Syringes Flow Rate: Remote HPSI Remote Minimum 1.56 pl/min 1.56 pl/min 3.06 pl/min 3.06 pl/min nl/min nl/min Maximum ml/min ml/min ml/min ml/min ml/min ml/min Connectors: USB (PC-to-Pump) Type B Type B Type B Type B Type B Type B RS-232 (PC-to-Pump) 9-Pin D-Sub 9-Pin D-Sub 9-Pin D-Sub 9-Pin D-Sub 9-Pin D-Sub 9-Pin D-Sub RS-485* (Pump-to-Pump) IEEE-1394, 6 pos IEEE-1394, 6 pos IEEE-1394, 6 pos IEEE-1394, 6 pos IEEE-1394, 6 pos IEEE-1394, 6 pos I/O & TTL 15-Pin D-Sub 15-Pin D-Sub 15-Pin D-Sub 15-Pin D-Sub 15-Pin D-Sub 15-Pin D-Sub Average Linear Force 34 kg (75 lbs) 34 kg (75 lbs) 91 kg (200 lbs) 91 kg (200 lbs) 200 kg (433 lbs) kg (1,000 lbs) Maximum Pressure (with 100 ml Syringe) 50 psi 50 psi 135 psi 135 psi 290 psi 670 psi Dimensions 30.5 x 21.6 x 17.8 cm 30.5 x 21.6 x 10.6 cm 30.5 x 21.6 x 10.6 cm 30.5 x 21.6 x 18.5 cm 30.5 x 21.6 x 10.6 cm 30.5 x 21.6 x 10.6 cm Control Box (12 x 8.5 x 7 in) (12 x 8.5 x 4.3 in) (12 x 8.5 x 4.3 in) (12 x 8.5 x 7.3 in) (12 x 8.5 x 4.3 in) (12 x 8.5 x 4.3 in) Dimensions 30.5 x 14 x 16.5 cm 30.5 x 14 x 17.8 cm 41.9 x 30.5 x 19.1 cm 41.9 x 30.5 x 19.1 cm N/A N/A Remote Box (12 x 5.5 x 6.5 in) (12 x 5.5 x 7 in) (16.5 x 12 x 7.5 in) (16.5 x 12 x 7.5 in) Weight 4.5 kg (10 lb) 4.8 kg (10.5 lb) 5.4 kg (12 lb) 7.7 kg (17 lb) 13.6 kg (30 lb) 20.9 kg (46 lb) CONSTANT PRESSURE S Y R I N G E P U M P Voltage Range VAC; 50/60 Hz VAC; 50/60 Hz VAC; 50/60 Hz VAC; 50/60 Hz VAC; 50/60 Hz VAC; 50/60 Hz Order # * Option for RS-232 (RJ-11, Pump-to-Pump) must be ordered at time the pump is ordered. ** Maximum pressure will vary with syringe size and applied force To order call techsupport@harvardapparatus.com

Contact Information CONSTANT PRESSURE S Y R I N G E P U M P UNITED STATES Harvard Apparatus 84 October

0664 toll free 800.599.4203 (USA Only) fax 203.776.1278 support@warneronline.com www.harvardapparatus.

1R9, Canada phone 514.335.0792 800.361.1905 (Canada only) fax 514.335.3482 sales@harvardapparatus.ca www.

Attn: Sales Department 6 Avenue des Andes Miniparc Bat. 8 91952 Les Ulis Cedex, France phone 33.1.64.46.

Germany phone +49 (0)7665 92000 fax +49 (0)7665 920090 info@hugo-sachs.de www.hugo-sachs.de SPAIN Panlab, S.

697 (International Sales) phone 934.190.709 (Sales in Spain) fax 34.934.750.699 info@panlab.com www.

6 Contact Information CONSTANT PRESSURE S Y R I N G E P U M P UNITED STATES Harvard Apparatus 84 October Hill Road Holliston, MA, 01746, USA phone toll free (USA Only) fax techsupport@harvardapparatus.com Warner Instruments 1125 Dixwell Avenue Hamden, CT, 06514, USA phone toll free (USA Only) fax support@warneronline.com CANADA Harvard Apparatus Canada Attn: Sales Department 6010 Vanden Abeele Saint-Laurent, Quebec, H4S 1R9, Canada phone (Canada only) fax sales@harvardapparatus.ca FRANCE Harvard Apparatus, S.A.R.L. Attn: Sales Department 6 Avenue des Andes Miniparc Bat Les Ulis Cedex, France phone fax info@harvardapparatus.fr GERMANY Hugo Sachs Elektronik / Harvard Apparatus, GmbH Gruenstrasse 1 D March-Hugstetten, Germany phone +49 (0) fax +49 (0) info@hugo-sachs.de SPAIN Panlab, S.L. / Harvard Apparatus Spain C/Energia, Cornellà, Barcelona, Spain phone (International Sales) phone (Sales in Spain) fax info@panlab.com SWEDEN CMA Microdialysis, AB Box 2 SE Solna phone fax cma@microdialysis.se UNITED KINGDOM Harvard Apparatus, Ltd. Attn: Sales Department Fircroft Way, Edenbridge Kent TN8 6HE, United Kingdom phone fax sales@harvardapparatus.co.uk

Research Grade Blood Pressure Transducer

Research Grade Blood Pressure Transducer USER S MANUAL Research Grade Blood Pressure Transducer, 110 VAC/60 Hz Research Grade Blood Pressure Transducer, 220 VAC/50 Hz MA1 72-4496 MA1 72-4497 Publication