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1 Checklist for validation of Biosafety Level 2 Laboratory (P2) (according to annexe 2 of the Directive concerning the internal procedure for projects subject to tification/authorisation according to the ContainO, RO and OPTM Ordinances Polylex 1.5.3) DESCRIPTION OF ACTIVITY Title Person responsible Building and room/laboratory/premises number Type of premises COSEC in charge of premises Project tified to Federal Coordination Centre for Biotechlogy Notification number: See EPFL Biosafety decision INFRASTRUCTURES 1 Access control via CAMIPRO Requisition N o : 2 Signs: Biohazard symbol 3 No entry for unauthorised persons symbol 4 List of authorised persons displayed on door 1
2 5 Door chart 6 Air pressure on premises NB: Not authorised to be in positive pressure in relation to adjoining corridor. Positive pressure Atmospheric pressure Negative pressure 7 Sealed windows NB: ContainO and ATCC regulation. If windows cant be sealed, they must be closed while work is being carried out in P2 zone 8 Autoclave in the building with maintenance procedure 9 Floor that can be easily cleaned with a disinfectant See possible alternatives (Anios or Biosanitizer). 10 Bench resistant to chemicals: Water, acids, bases, solvents, disinfectants, decontaminants 11 Clean work areas for deposit of items or materials before exiting P2. EQUIPMENT & SAFETY EQUIPMENT 12 Man down system (DATI) Requisition N : 13 Microbiological safety cabinet (BSC Class II for human pathogen manipulation) BSC Class I BSC Class II BSC Class III 2
3 with maintenance procedure None Check completed on: 14 Vacuum pump and autoclave-resistant bottles Treatment: 15 Rotors and buckets with safety lid NB: Reminder all buckets or rotors for centrifugation must be used with their safety lids. If the tubes were closed during centrifugation and problems were experienced during the process, it is t necessary to transport the buckets and rotors under a hood to open them. 16 Pipets and pipet-tips with aerosol protection 17 Autoclavable bins and bags NB: Alternatively, it is possible to use medical sachets for waste products. When the trials are finished, the sachets must be sealed and discarded in the main bin. 18 Telephone 19 Emergency eyewash unit 20 Fire extinguisher NB: Fire extinguisher is available in the corridor outside the laboratory. 21 Decontamination and cleaning equipment WARNING: Biocidal is t effective against nenveloped viruses. 22 Tracking system for cleaning (logbook or other) 3
4 PERSONAL PROTECTION 23 Cotton lab coat or surgical gown with long sleeves and tight cuffs and procedure to clean and autoclave them 24 Respiratory protection NB: For activities during which use of a BSC is t possible, it may be requested that a mask is worn, e.g. stereotaxic injections. Reason: 25 Safety goggles or face mask Reason: 26 Gloves (EN 374/2 and EN 374/3) NB: if possible, begin using nitrile gloves and stop using latex gloves. 27 Hand disinfectant Work organisation 28 Training courses Training instructor: 29 Written work instructions provided to users either electronically or on paper, or displayed in the laboratory. 30 Organism inventory 31 Management of the storage of organisms 4
5 32 Other visitors NB: visitors are followed by the Biosafety Officer or staff members EPFL BIOSAFETY DECISION Laboratory fulfils requirements Authorisation for omission of safety measures must be requested Substitute measure: Activity authorised by EPFL Biosafety conditional Justification for conditional or nauthorised activity: Work or tasks to be carried out Point Description of work or tasks to be carried out In charge Date Signature, EPFL Biosafety Name : 5
1. The laboratory supervisor must enforce the institutional policies that control access to the laboratory.
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