Laboratoires Dom AVMM (Suisse) Inc. New Innovation in Peptide Therapy for Slimming

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1 Laboratoires Dom AVMM (Suisse) Inc New Innovation in Peptide Therapy for Slimming

2 Globally, Fat Facts 1 billion overweight adults 300 million obese adults A major risk for chronic diseases: Type 2 diabetes Cardiovascular disease Hypertension Stroke Cancer

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4 Biomed Environ Sci 2002;15: 83 95

5 What is Myostatin? Cytokine - small signaling protein Produced in skeletal muscles Controls cell proliferation and differentiation Restrains muscle growth

6 Cytokines - Intercellular Signals Coordinate the function of individual tissues for the entire organism. Control tissue growth, development and function. Molecular signals lead to a ultimate response in or by the cell.

7 Three Muscle Systems 1 Smooth Muscle 2 Cardiac Muscle 3 Skeletal Muscle

8 Myostatin Blocks Myogenesis Increases Adipogenesis Myogenesis- the formation of muscle tissue Adipogenesis- the formation of fat tissue

9 Clinical Study: Myostatin Inhibits Myogenesis and Promotes Adipogenesis Myostatin promotes the differentiation of multipotent mesenchymal cells into the adipogenic lineage and inhibits myogenesis. Division of Endocrinology Metabolism and Molecular Medicine (J.N.A., S.B., S.R.-P., R.S., N.F.G.- C.), RCMI DNA Molecular Core, Charles R. Drew University of Medicine and Science, Los Angeles, California 90059

10 Myostatin promotes Fat Growth

11 Role of Myostatin in Slimming Myostatin negatively regulates muscle growth and fat growth Inhibitors targeting myostatin are actively being sought Therapeutics treatment of muscle-wasting, cachexia, muscular dystrophy and sarcopenia (Bradley et al, 2008; Tsuchida, 2008).

12 Myostatin blocking in Mice In 1997, Se-Jin Lee of Johns Hopkins University School of Medicine in Baltimore, Maryland. The animals grew muscles twice as big as normal.

13 Myostatin blocking Muscle Dissection

14 Se-Jin Lee Johns Hopkins University School of Medicine mice engineered to lack myostatin exhibit dramatic increases in skeletal muscle mass throughout the body. If you could increase or maintain muscle strength as people age, you could have a tremendous impact on health and well-being.

15 Myostatin Blocked in Dogs

16 This bull has been bred to be predisposed to Myostatin blocking

17 Double Muscled Sheep

18 Super Strong Kids May Hold Genetic Secrets By LAUREN COX ABC News Medical Unit April 2, 2009 At 5 months old, Liam Hoekstra of Grand Rapids, Mich. started doing an expert move called the iron cross. By 8 months he could do a pull up.

19 Myostatin Inhibitor Peptide Follistatin Isoform: Follistatin blocks myostatin. Research has shown that the blocking of myostatin, by genetic elimination or by increasing the amount of follistatin, results in greatly decreased adipogenesis.

20 Clinical study: Changes in muscle myostatin expression in obese subjects after weight loss myostatin-deficient mice have a significant reduction in fat deposits and a depression of adipogenesis Journal of Clinical Endocrinol Metabolism Jun;89(6):

21 Increased muscle mass of senescent (24-month-old) mice with Myostatin blocker (Myopep)

22 Toxicity of Myostatin Blocker (Myopep) Sub acute toxicity trials of Myopep daily in rats (at 100 times recommended dose) showed no gross organ pathology or negative behavioral effects

23 Does Mysostatin deficiency effect the heart muscles? no cardiac developmental abnormality has been reported in myostatin-null mice (1) There was no statistically significant difference between the heart weight/body weight ratios of myostatin-null mice when compared to wild-type mice (2) (1) McPherron AC, Lawler AM., S.J. L Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature. 1997;387: (2) Myostatin Does not Regulate Cardiac Hypertrophy or Fibrosis Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland

24 Clinical Study: Organization of human myostatin gene and expression in healthy men and HIV-infected men with muscle wasting. concentrations of myostatin are increased in HIVinfected men with weight loss compared with healthy men myostatin is an attenuator of skeletal muscle growth in adult men and contributes to muscle wasting in HIVinfected men. * Division of Endocrinology, Charles R. Drew University, Los Angeles, CA 90059; Division of Endocrinology, Washington University School of Medicine, St. Louis, MO 63110; University of Toronto, Toronto, Ontario, M5S1A1 Canada; and ** Karolinska Institutet, Stockholm, SE Sweden

25 Clinical study: Myostatin blocker reduces body fat, fasting blood sugar, bad cholesterol and triglycerides The mice with deleted myostatin gene: 30% less body fat 80% lower fasting insulin levels. 50 % lower low-density-lipoprotein ("bad") cholesterol 30 to 60 percent lower levels of total cholesterol and triglycerides *These results indicate protection against the development of atherosclerosis. Taylor WE, Bhasin S, Artaza J, Byhower F, Azam M, Willard DH Jr, Kull FC Jr, Gonzalez-Cadavid N. Myostatin inhibits cell proliferation and protein synthesis in C2C12 muscle cells. Artaza JN, Bhasin S, Magee TR, Reisz-Porszasz S, Shen R, Groome NP, Fareez MM, Gonzalez-Cadavid NF. Myostatin inhibits myogenesis and promotes adipogenesis in C3H 10T(1/2) mesenchymal multipotent cells. Endocrinology.

26 Clinical Study: AAV-dependent targeting of myostatin function: Follistatin strikes back at muscular dystrophy A single injection of the myostatin inhibitor proteins enhanced locally the muscle mass for approximately 2 years. Haidet AM, Rizo L, Handy C, Umapathi P, Eagle A, Shilling C et al. Long-term enhancement of skeletal muscle mass and strength by single gene administration of myostatin inhibitors. Proc Natl Acad Sci USA 2008; 105:

27 In conclusion: Myopep induced muscle growth. The hypertrophic effect results from inhibition of Myostatin The striking ability of Myopep to enhance muscle growth warrants consideration as a regulator of muscle mass

28 Myopep-Myostatin Peptide Injectable Peptide: Australian Manufactured Accelerates Muscle Growth Blocks the Enzyme that Limits Muscle Growth Self Limiting No Side effects Myostatin Deficiency

29 Myopep for Slimming Myopep Turns off the Creation of Fat Myopep Turns on the Building of Muscle

30 Myopep for weight loss: One Subcutaneous injection a week 1ML for 10 weeks Sharon lost 25 lbs. in 10 weeks With no exercise or dietary changes

31 Myopep for Slimming BEFORE AFTER

32 Myopep for Slimming BEFORE AFTER

33 Myopep for Weight Loss BEFORE AFTER Kathy has lost 25lbs in two months. Her body fat is down from 36.4 to 29.7 as of 11 th January Her diet is the same as before. She does 20 minutes of jump rope every other day and some light exercises. She reports having more energy and being able to walk twice as far as before.

34 One of the most promising ways of increasing strength is by blocking a protein called myostatin Given all that biological evidence, a drug that blocks myostatin would seem like a slam dunk as a treatment for muscular dystrophy

35 Myostatin Drugs are possibly 4 times more effective than high doses of steroids in growing muscle The inoculations prevented muscle deterioration in mice as old as 2 years years in human terms The shots even regenerated muscle, restoring some of the lost strength and size. Old mice regained 27 percent of muscle lost to age; younger mice experienced a 15 percent increase

36 Body Building Testimonial After 4 weeks on Myopep: My muscles are looking full and hard, maturity ever so evident. I can see further fat loss. I believe that more neuron pathways have been recruited my muscle fibers are growing and repairing at an incredible rate. No DOMS (Delayed onset muscle soreness) HAVE BEEN EXPERIENCED THUS FAR post workout.

37 BEFORE AFTER

38 Myopep and Anti-aging

39 Sarcopenia- Degenerative loss of skeletal muscle mass and strength with aging -.5-1% per year Source: J Nutr Health Aging Aug-Sep

40 Aging Body Fat Increases/ Muscles Diminish Cohn SH. New concepts of body composition. In: Ellis KJ, Yasumura S, Morgan WD (eds). In Vivo Body Composition studies. The Institute of Physical Sciences in medicine: London, 1987, pp1-14.

41 Aging Gracefully

42 Veterinarian Applications BEFORE AFTER

43 Applications in Veterinarian Medicine

44 Treatment for Obese Pets

45 *Myopep will be officially launched in An oral supplement form will be commercially available in May 2012*

46 For more information on Myopep visit: or us at

47 Legal Disclaimer Laboratoires Dom AVMM (Suisse) Inc. has taken strict measures to safeguard the reliability of the information contained herein. Neither Laboratoires Dom AVMM (Suisse) Inc. nor its partners can provide any assurance or warranty, including to third parties, in relation to the accuracy, reliability or completeness of the presented information. All information contained in this presentation is intended for informational and educational purposes. The information is not intended nor suited to be a substitute for professional medical treatment or for professional medical advice. We urge you to always seek the advice of your physician or medical professional with respect to your medical condition or questions. 47

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