Yevgeni Tomsov My Dad
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- Beverly Stanley
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3 Yevgeni Tomsov My Dad
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5 1 million people!
6 61 million people!
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8 Male pattern baldness by the age of 35 45% 55% N=470 Suffer from baldness symptoms Don't suffer Practices
9 How effective are the solutions available? 7% 93% Practices Effective Not effective N=470
10 #trumpcat Hair tattoo Jill Carlson, flickr Hagai Dekel, ynet
11 Surgical Non-surgical
12 Non-surgical treatment Other 44.3% Topical indirect 28.2% Oral direct 27.5% Relevant Research, Inc. International Society of Hair Restoration Surgery 2014
13 Non-surgical treatment Topical indirect Relevant Research, Inc. International Society of Hair Restoration Surgery 2014
14 Non-surgical treatment Oral direct Side effects Relevant Research, Inc. International Society of Hair Restoration Surgery 2014
15 The cause 5α-reductase Testosterone Dihydrotestosterone
16 Dihydrotestosterone (DHT)
17 Surgical Non-surgical
18 NADH/NADPH NAD + /NADP + 3α-hydroxysteroid dehydrogenase DHT 3α-Androstanediol Human 3-alpha hydroxysteroid... The Journal of Steroid Biochemistry and Molecular Biology 141,
19 Bacillus subtilis: 15.4% The most widespread bacteria on the scalp Secretes Roia, F.C.; Vanderwyk, R., R. W.. Journal of The Society of Cosmetic Chemists. 1969, 20,
20 Would balding men use a product which includes genetically modified bacteria? 22% 78% Practices Yes No/don t know/other N=470
21 NADH/NADPH NAD + /NADP + 3α-hydroxysteroid dehydrogenase DHT 3α-Androstanediol Human 3-alpha hydroxysteroid... The Journal of Steroid Biochemistry and Molecular Biology 141,
22 For B. subtilis
23 NADH/ NADPH NAD + / NADP + 3α-hydroxysteroid dehydrogenase DHT 3α-Androstanediol Human 3-alpha hydroxysteroid... The Journal of Steroid Biochemistry and Molecular Biology 141,
24 F. Canonaco, et al. FEMS Microbiology Letters 204 (2001)
25 F. Canonaco, et al. FEMS Microbiology Letters 204 (2001)
26 F. Canonaco, et al. FEMS Microbiology Letters 204 (2001)
27 F. Canonaco, et al. FEMS Microbiology Letters 204 (2001)
28 F. Canonaco, et al. FEMS Microbiology Letters 204 (2001)
29 NADPH NADPH NADPH NADPH NADPH NADPH NADPH F. Canonaco, et al. FEMS Microbiology Letters 204 (2001)
30 For BL21 For MG1655
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32 Bald DHT Chassis Cofactor Results Comb Practices For B. subtilis For MG1655
33 Secretion Assay Principle MIT OpenCourseWare evrogen.com
34 Normalized fluorescence (a.u.) Secretion with signal peptide apre from B. subtilis mcherry signal peptide+mcherry Time (hour) BBa_K
35 Activity assay principle In silico model: MIT OpenCourseWare George H. Patterson et al. PNAS 2000;97:
36 Fluorescence (a.u.) Activity of 3αHSD in E. coli BL21 lysate, DHT=40 µm αHSD no 3αHSD In silico model: Time (min) BBa_K
37 Reaction Rate (1/sec) Bald DHT Chassis Cofactor Results Comb Practices Reaction rate of NADPH consumption in different DHT concentrations 0.4 3αHSD no 3αHSD In silico model: DHT (µm) BBa_K
38 Cofactor assay principle Extracellular Intracellular MIT OpenCourseWare
39 Fluorescence/OD (a.u.) Fluorescence/OD (a.u.) Bald DHT Chassis Cofactor Results Comb Practices Normalized NADPH fluorescence vs. Time for E. coli BL21 strains BL21 wildtype BL21 with zwf BL21 wildtype BL21 with zwf Time (hour) 0 BBa_K
40 Combined experiment principle Intracellular Extracellular MIT OpenCourseWare
41 Reaction Rate (1/sec) Bald DHT Chassis Cofactor Results Comb Practices Reaction rate of consumption of NADPH from enriched supernatant Combined experiment αHSD no 3αHSD BBa_K BBa_K
42 ?
43 The Comb Design Clean Reusable Safe Uniform application
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48 Optimal storage conditions: -18 C in 80% glycerol medium Practices
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50 Benefits of our solution Purified enzyme solution vs. bacterial secretion NADPH User experience
51 Achievements New Application BioBricks Design Modeling Collaboration Practices
52 Our Mentors: Dr. Roee Amit Dr. Orna Atar Noa Katz Inbal Vaknin Lior Levy Alex Ereskovsky
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