Proceedings of the 11th International Congress of the World Equine Veterinary Association
|
|
- Alvin Dixon
- 5 years ago
- Views:
Transcription
1 Proceedings of the 11th International Congress of the World Equine Veterinary Association September 2009 Guarujá, SP, Brazil Next Meeting : Nov. 2-6, Hyderabad, India Reprinted in IVIS with the Permission of the Meeting Organizers
2 Contemporary aspects of Embryo Transfer; a Brazilian perspective. MP Reprodução Eqüina Ltda Bauru-São Paulo- Brasil MV Marcelo de Araújo Pessôa MV André de Paula Cannizza MV Gabriel Felício Introduction: MP Reprodução Equina is a partnership, localized in Bauru, middle-west of São Paulo State, which works mainly with equine reproduction. History: The commercial embryo transfer program, was started in Brazil, by João Fleury, in 1985, using the surgical ET Technique. In 1996, the non-surgical technique became more popular and, now, we do not have any news about someone using surgical technique in Brazil. In 2008/09 breeding season approximately of embryo transfers were made, becoming Brazil one of the leaders in number of ET in the world. We started, in 1996, our commercial program using non-surgical transfer. That year, we got our first ET pregnancy after 13 flushing and 7 transfers. It was a little frustrating. In 1997, we got better results, and then our program started getting more clients, making more flushing e getting more pregnancies. Last breeding season we finish with 523 pregnancies at 40 days, after 1152 flushing. From 1997 to 2008, we change the main breed worked, going from PH to QH. We started making a good number of pregnancies, using clients recipients, and flushing a lot of mares out of our breeding center. At this time bigger we were more problems we found. The Routine: In Brazil, depending the breed involved the procedure to manage the donor mares to be flushed is different. The most vets involved with Mangalarga, Mangalarga Marchador and Campolina breeds manage the donor mares in the client s Ranch. The most of those Ranches own their recipients and all the procedure is realized there. In the QH, PH, Jumping breeds the most of donor mares, are shipped to breeding center to be managed. We are working on both type of management. We are 3 vets, involved on the service. We own our breeding center, localized in the family Ranch owned, and we take care of 16 more different Ranches. Our routine includes: palpations, inseminations, collection of semen to ship or fresh use, flushing mares and transferring embryos. In all of those places, we work in an every other day schedule. In small ranches, where we have a more malleable schedule, the visit is not so frequent. On those Ranches we flush some mares, but the most of then will finish the year pregnant. We manage some popular QH stallions and around 200 doses of semen are shipped every month from August to February. We also received about 40 % from the semen we to use to inseminate the mares. We have to work every day, even Sundays and holidays from august to February. It happens, with the most of vets involved with equine reproduction in Brazil. In our practice, we still were not able to change that, to provide us at least one day off every week. The Breeding Center routine: We worked at our Breeding Center with about 120 client mares and 15 owned by the Ranch. About 35 of them finished the breeding season carrying your own baby, some after were flushed to produce ET pregnancies. The recipient mares owned by us are kept in our breeding center. We can keep around 160 recipient mares at the same time. They are kept in dry lots, with 15 mares housed in each
3 one. The recipient herd has been feed with hay, grain and barley. 328 ET pregnancies were done in this herd in 2008, including embryos collected in the breeding center or collected outside and transported to be transferred at the Center. In the morning we evaluate the mares, collect the stallions to inseminate or ship the semen, flush the mares, and do the transfers. Every veterinary involved, has their schedule, previously, determined. Everything has to be done on this day. We palpate in the non-breeding day only mares to be bred with shipped or frozen semen. Also the recipients which will be transferred in non-breeding day, have to be checked on breeding day. In the other day, we work in a big Ranch that kept around a 100 recipient mares at the same time, and about 170 breeding mares. On this place were made 150 ET pregnancies and 110 mares are carrying your own babies. This Ranch owns his stallions, donor mares, recipient herd, and also provides services for his clients. In the afternoon, we have to visit the small ranches, palpating mares, collecting stallions, doing some clinic and flushing mares. Almost all these embryos collected, are transported to be transferred at our breeding center. The Brazilian model of producing embryos: - Pre-Breeding Season Management (Artificial Light Treatment ): Around April 15 th, 150 recipient mares are available to begin our program. They are divided in 15 mares by lot and kept in a dry lot. Were chosen following the criteria: returning mares which have foaled an ET foal or new mares bought at least 90 days earlier and adapted to our management. Had been fed in the same way and they will not change their lot until get pregnant. The light program started on May 20 th, every year since from We tried, in 2004, start the lights in winter solstice (around June, 10th) as is recommended by literature, but the recipients started cycling around 60 days later. It was a little late to our program, because in middle of July we already have some mares to flush. The light program has worked pretty well for us. We strongly think that the adaptation time is responsible for the good results we have early in the breeding season. We chose the mares which we think were the best ones, they are cycling early, they are adapted, and this is the only time in the breeding season, we have a lot of ovulated recipients to choose. In breeding season 120 recipient mares were put on lights on May, 20th. In the first palpation between July, 10th and 21st almost 80% of them were cycling, 80% got pregnant in the 1st ET and more than 90% get pregnant at all. (see table 1) Table 1. No. of mares Rate Total mares 120 Cycling middle 94 78,3% of July Not cycling 26 21,66% middle of July Do not cycle at 5 4,16% all Pregnant 1 st. ET 96/119 80,6 % Pregnant 2 nd.et 21/22 95,45% EED 1 st. ET 8/96 7,39% - Reproductive management of donor mares: The donor mares nutritional management and housing is Ranch responsibility. We
4 orientate it, looking for the best, but daily board is charged by the Ranch. We also put the most of donor mares housed in the Ranch on the lights. The donor mares are evaluated every other day. All of management is recorded. When ready to be bred, the ovulation is induced using the most of the times Deslorelin (1mg, IM). If fresh semen is to be used, they will be inseminated on the same day, if cooled semen is the choice it will be done on the same day or the next day depending of the stallion s schedule. The ovulation is induced earlier, if the cooled semen will take more than 12 hours to be used after the collection. The goal, to this cooled semen, is the mare ovulate no more than 24 hr post AI. The necessary uterine treatments can be done in the morning or in the afternoon, depending of the problem of the donor mare. Between 7 and 9 days, after the ovulation is detected, we flush those mares to look for an embryo. On this way we work, we try pay a lot of attention when are checking the follicles. If we think the mare can ovulate soon, we plan to flush her 7 days after the ovulation is detected by palpation. Sometimes the donor mare had detected its ovulation late and a big embryo can be flushed (until 6 mm of diameter). -Reproductive management of recipient herd: The recipient mares feeding management and housing is the Breeding Center responsibility. The recipients are identified by numbers forged in the croup. They are evaluated every other day. We record the results from the palpations, and mainly the ovulation. As we do not palpate every day, we avoid use mares recorded with 8 days pos-ovulation, at least a fresh ovulation was palpated. If the schedule is so tight, we plan to transfer the embryos on these mares 7 days after they have the ovulation determined. As a lot of transported embryos are transferred, and it is done normally on the nonbreeding day, the recipients are checked one day earlier in the breeding day. We do not make any uterine treatment in recipient mares. We just use prostaglandin (as a luteolytic agent ), oxytocin (as a ecbolic agent ) and deslorelin ( to induce ovulation ). Most of the mares that not are pregnant after the second ET are eliminated from the program. In a few occasions we can give them one more chance. It includes mares that foaled by ET before, mares recently bought who had a hard time (a lot of stress) to adapt to our program, or when we do not get buy mares to replace the pregnant ones. But we try all the time be so selective. More chance we give worse will be our results. We are absolutely conscientious that the stress of the recipient mares is the most important deleterious problem in any ET program. It can be caused by nutrition, environmental temperature, rain, diseases, or other factors do not determined yet. In our program, as we can not keep at the same time more than 150 mares, we have to replace the pregnant mares around 90 days after we initiate the breeding season (middle of October). It causes a big problem to us. At this time we have to buy mares in bad body condition, they never been in a management like ours, eating hay, grain and barley, and the most are not cycling. And a lot of them will experiment Strangles Disease. Quarantine can not be done, because at this time of the year about 65% of the recipients that started the breeding season are already pregnant, and a lot of donors are available to be worked. Commonly in November, the pregnant rate goes abruptly down. After that some increase happens, but never more as the breeding season were initiated. ( Graph 1 ). Graph 1
5 Comparison between months 100% 80% 60% 40% Pregnancy rate EED rate 20% 0% aug/08 sep/08 oct/08 nov/08 dec/08 jan/09 feb/09 mar/09 - ET procedures from collection to transfer: The technique is familiar to everybody. In our practice, mare s flushing, embryo manipulation and transferring it to the recipient is almost 100% of the times done for the same vet. We do not use to sedate the donor mares. A filter in line which is opened in the top is our preference. We had problem before recovering embryos ruptured during the flushing and decided to do not use negative pressure in the filter. The cuff of the collection catheter is inflated with 35 cc air approximately, that becomes easier if the repositioning of the catheter in uterus need be done. To flush the mare we use Lactated Ringer solution warmed between C. One liter each flush is used, no more than 3 flush. As much as possible, of the solution, needs to be recovered. After finish the flush, if the fluid recovered is cloudy we wash it with another liter of LR, until it looks clear. 30 cc of fluid is kept in the filter; it is rotate in a non-clock wise and deposited in a petri dish. More 30 cc of LR is deposited in the filter and the procedure is repeated. We also rotate the petri dish in a non-clock wise for many times trying to move the embryos for the center of the dish. Smaller is the embryo easier to move it to center. If embryo can not be localized at the center of the petri dish we search in all the dish. If an embryo is in the petri dish most of the times it is not necessary. After the embryo is visualized it will be manipulated. A holding solution named Holding Plus (Vitrocell) is our preference. It s 0,4% BSA solution. We wash the embryo on this solution for seven times. If the fluid recovered was not clear we prefer wash a lot of more times, at least 20. Then the embryo is kept in a cryogenic tube with 2,0 cc of holding. The embryo can be maintained on this tube, at room temperature, until 4 hours. If it will take longer we prefer refrigerate it until degree Celsius. The embryo transfer is realized in a recipient mare previously examined. We look for a good uterine and cervix tonus, and a homogeneous image of the uterus. We prefer mares that are d4 to d7 post-ov. All of our recipients receive a dose of acepromazine ( 20 mg i.m. ) just before the transfer. The embryos until 1400 microns of diameter are transferred using a stainless steel reusable insemination gun from IMV, embryos from microns of diameter are transferred using AI pipette. Bigger than that we have adapted swab devices. The transfer procedure must be realized fast and without any damage to the cervix and uterus of the recipient mare. We think easier and fast you can do is better. We localize the cervix, do not introduce the finger in the external os, just the insemination gun. If necessary, the cervix
6 can be manipulated from the vagina and the embryo is left into the uterine body. No attempt to reach the uterine horn is done. The mares are checked 4 to 6 days later and if they are pregnant they are taken out from the lot and go to a pregnant lot. Our intention is decrease the movement of those mares. They are check again once time a week until 40 days, and then they go to new home. It is important to say, a lot of clients in Brazil do not wish take their mares to a Breeding Center, then all the service has to be provide in their Ranch. No laboratory does exist in most of these places. The work has to be done in the same way, and it is true to the most of vets involved with equine embryo transfer in Brazil. Results and comparisons: Between breeding centers we manage: During 2008/09 breeding season we managed a large number of recipient mares in two different places. One was on our breeding center where we manage the mares like we think would be better. On the other place, the recipient herd was kept in a low quality pasture with no supplementation. Around 100 mares are kept together in the same pasture. Has no division between the herds until they get pregnant. In both places the recipient replacement is done with mares in bad body condition. But they take longer to get better in the second place. Also we had at this farm a lower pregnancy rate and higher EED. ( see table 2 ). It was considered by us, as a result of poor general management. Table 2. MP Reprodução 2º. Breeding Total Equina Center Center No. ( % ) No. ( % ) No. ( % ) ET Preg 12 days 370 ( 75,35 ) 178 ( 69,26 ) 598 ( 73,70 ) Preg 40 days 328 ( 66,80 ) 150 ( 58,36 ) 523 ( 64,48 ) EED 42 ( 11,35 ) 28 ( 15,73 ) 75 ( 12,54 ) Lactating, cycling, non-cycling mares: When available, a lactating mare can be used as a recipient. Normally they are recipients that foaled in clients farm where we follow then. It saves money to clients, because will feed a mare pregnant and lactating and does not need introduce a new mare in their herd. Also we do not have to look for new recipients to replace our herd, increasing their use in our program. A smaller pregnant rate in lactating group was observed comparing with cycling nonlactating group, but the EED rate was similar. In all the places we had used the lactating recipient; they were kept with the breeding mares of the farm. They are kept on good pastures, are supplemented with grain, and we do not transfer embryos on them until they have ovulated twice pos-partum. Even the results are little lower than with non-lactating mares the advantages surpasses the disadvantages. Embryos are also transferred in non-cycling progesterone ( P4 ) supplemented mares. They were used in large number at our program, until Then, when the artificial lighting program were started, the number of P4 treated mares decreased a lot. However, after four years, with the increase in number of transfers, the number of non-cycling P4 treated mares increase again. Follow bellow a comparison between the results using cycling non-lactating, non-cycling P4 treated and lactating mares. (table 3) Table 3.
7 Cycling recipients Non-cycling recipients P4 treated Lactating recipients total No. % No. % No. % No. ( % ) ET 517 ( 63,7 ) 209 ( 25,7 ) 85 ( 10,5 ) 811 ( 100 ) Pregnant 12 days 415 ( 80,2 ) 130 ( 62,20 ) 53 ( 62,35 ) 598 ( 73,73 ) EED 48 ( 11,56 ) 21 ( 16,15 ) 6 ( 11,32 ) 75 ( 12,54 ) Pregnant 40 days 367 ( 70,98 ) 109 ( 52,15 ) 47 ( 55,29 ) 523 ( 64,48 ) Our protocol involved the use of Estradiol Benzoate ( 3mg IM, s.i.d for 2 days ) and then a shot of long-acting progesterone ( 10cc of 300mg/ml P4- Biotech ). This year, the shot of progesterone was repeated every 14 days and maintained until around 105 days of gestation. The pregnancy rate at 12 and 40 days was lower when comparing with the cycling mares, also higher was the EED in P4 treated mares. It can be explained, because on this year the most of non-cycling mares used in P4 treatment were recently bought, were in a bad body condition and had no time to adapt to our management. It can be clearly noted when we compare the pregnancy rate monthly. ( graph 2 ) Until middle of October a pregnant rate was higher than 70% then came down in November for less than 40 %. Until October, we were transferring mares which were in our management for at least 90 days, but they were not cycling. After November the pregnant rate could not sustain. At this time, the mares recently bought were introduced into the program. Those are in bad body shape, were not cycling (which denote they were not in good condition). The program got up and down. It did not decrease the importance in using this protocol, because without this we would not be able to produce these pregnancies. Graph 2. P4 supplemented mares ET number ,00 80,00 70,00 60,00 50,00 40,00 30,00 20,00 10,00 0,00 % ET preg rate 12 d EED aug/08 sep/08 oct/08 nov/08 dec/08 jan/09 feb/09 mar/09 Fresh and cooled semen: As previously described around 40 % of donor mares are inseminated with cooled semen. This semen comes from different places of the Country. It is normally package in a insulated Styrofoam box which keeps the temperature around 16 degrees Celsius. It can be transported by mail, bus, air or Moto boys. The Moto boys are very common in Brazil. We believe the problems find out in some cooled semen are responsible for the smaller recovery rate in comparison with the fresh semen. We also work with a small amount of frozen semen.
8 Follow table 4 for comparison of the embryo recovery rate between fresh, cooled and frozen semen. Table 4 Fresh semen Cooled semen Frozen semen total No. of AI Flushs Recovery rate 72, 2 % 58,9 % 43,8 % 65,5% Training and non-training mares: Also is common in Brazil, transfer embryos from mares that are training and competing in shows. In our area, a lot of Ranches breeds cutting, reining and barrel racing horses. They also keep mares in training and competition. During 2008/09 breeding season 39 mares in training, including 31 mares been showed at competitions, some of then top level competitors were flushed. Those mares are riding at least 4 times a week, and the schedule for the barrel racing mares includes be showed at least once a month. All those mares produced at least one embryo and one pregnancy during this the breeding season. The results were so similar when comparing with the breeding mares worked into the breeding season. (See table 5) Table 5 Training mares ( n= 39 ) Breeding mares Total No. ( % ) No. ( % ) No. % flush Recovery rate 96 ( 69,50 ) 661 ( 65,18 ) 757 ( 65,70) ET Pregnant 12 d 82 ( 78,09 ) 516 ( 73,08 ) 598 ( 73,73 ) Pregnant 40 d 73 ( 69,52 ) 450 ( 62,74 ) 523 ( 64,48 ) EED 9 ( 10,97 ) 66 ( 12,96 ) 75 ( 12,54 ) The Brazilian Perspective: From 1986 to 2008 breeding season the embryo transfer commercial program in Brazil has increased a lot. Alvarenga and Losinno (2006) reported that approximately ET were performed in Brazil in On last breeding season ( ) we estimated that ET were performed in our country. Since João Fleury started his program in 1985, the ET in Brazil has changed a lot. In the first 10 years the most of ET pregnancies were produced in Margalarga and Mangalarga Marchador breeds. With the time Campolina was incorporated and then the Jumping horses. From 1997 to 2004 a lot of ET pregnancies were done in Paint Horse breed using QH mares as a donor. At that time Quarter Horse was not allowing a multiple foals for the same mare in the same year. In 2005 ABQM changed the rules and allowed the register for the multiple foals
9 from the same mare. The number of ET in PH went down. The number of ET in QH has increased a lot from that. About 2500 ET pregnancies were produced this breeding season in QH. Below is the evolution on our commercial program since beginning. (See Table 6) Table 6 year Flush No. Recovery rate Preg. 12 days rate Preg. 40 days rate EED rate Efficiency rate % 14,3% 14,3% 0 7,6 % % 50 % 50 % 0 16,6 % ,5 % 75 % 65 % 13,3 % 40,6 % ,9 % 69,6 % 64 % 8 % 43,2 % ,6 % 73,1 % 60,8 % 16,75 % 38 % ,5 % 69,7 % 58,5 % 15,9 % 39 % ,8 % 74,1 % 63,7 % 14,1 % 42 % % 66 % 58 % 11,4 % 40,6 % % 72,66 % 62,68 % 13,73 % 41 % % 71% 63,1% 11,6% 40,4% ,9% 73,2 % 67,1 % 8,3 % 39,5 % ,71 % 73,7 % 64,48 % 12,54 % 45,39 % Total no ,54% Next year, with the new rules from ABQM it probably will decrease. Basically the association decided to charge more for embryos produced in mix breed mares, trying to stimulate the use of the pure breed recipient mares. Unfortunately, we are not able at this time to estimate the impact of this new rules on ET number. Anyway the Crioulo breed has just allowed the use of ET, and it is looking so promissory. Crioulo is the third breed in number of registered horses in Brasil. Also other smaller breeds, as Lusitano, are starting to use the technique.
1 Embryo shipped to ERC 3 Embryo Recovered by ERC. 2 Embryo Recovered by ERC 4 Embryo Recovered by ERC
EMBRYO TRANSFER - Options, Fees and Descriptions- Options 1 Embryo shipped to ERC 3 Embryo Recovered by ERC Donor stabled - none Recipient - supplied by mare owner Donor and recipient stabled - at ERC
More informationmore inside... TRACK TO HACK and AUSTRALIAN MINIATURE PONIES The best for your horse July 2014 EMBRYO TRANSFER JEN HAMILTON Part 6!
The best for your horse July 2014 Part 6! TRACK TO HACK Consolidating the Basics AUSTRALIAN MINIATURE PONIES EMBRYO TRANSFER What you need to know AU$4.95 ISSN 2200-3754 Show Jumping with JEN HAMILTON
More informationArtificial Insemination
Artificial Insemination Artificial Insemination (A.I) is a technique used to transfer semen from a stallion into the uterus of a mare during the correct stage of her oestrus cycle. A.I has become very
More informationBREEDING YOUR FIRST FOAL - A GENERAL OUTLINE OF WHAT IS INVOLVED. Written by Astek Stud, Nelson, New Zealand.
BREEDING YOUR FIRST FOAL - A GENERAL OUTLINE OF WHAT IS INVOLVED Written by Astek Stud, Nelson, New Zealand. When people come to us interested in breeding a foal, our first question to them is what sort
More informationCourse: Equine Science. Unit Title: Horse Breeding Techniques TEKS: 130.5(C)(3)(C) Instructor: Ms. Hutchinson. Objectives:
Course: Equine Science Unit Title: Horse Breeding Techniques TEKS: 130.5(C)(3)(C) Instructor: Ms. Hutchinson Objectives: After completing this unit of instruction, students will be able to: A. Explain
More informationCSU/ERL Embryo Transfer Contract 2013 Breeding Season
CSU/ERL Embryo Transfer Contract 2013 Breeding Season Department of Biomedical Sciences Animal Reproduction & Biotechnology Laboratory DONOR MARE INFORMATION Registered Name: Barn Name: Breed: Age: Registration
More informationBlau Sport Horses & Ponies.
Blau Sport Horses & Ponies TELEPHONE: 0488 163 511 EMAIL: alison@blau.com.au WEBSITE: www.blau.com.au BREEDING CONTRACT 2016 SEASON COMMANDER COHEN LIVE COVER Mare to be bred: Name of Mare: Breed: Registration
More information(a) The Breeder may not assign or transfer this Agreement or any interest in this Agreement. 2. STALLION.
2-Year Equine Breeding Agreement This Equine Breeding Agreement (the Agreement ) is entered into as of by and between Hilltop Farm, Inc., a Maryland corporation with its principal place of business at
More informationCSU/ERL Embryo Transfer Contract 2015 Breeding Season
CSU/ERL Embryo Transfer Contract 2015 Breeding Season Department of Biomedical Sciences Animal Reproduction & Biotechnology Laboratory DONOR MARE INFORMATION Registered Name: Barn Name: Breed: Age: Registration
More information(a) The Breeder may not assign or transfer this Agreement or any interest in this Agreement. 2. STALLION.
2-Year Equine Breeding Agreement This Equine Breeding Agreement (the Agreement ) is entered into as of by and between Hilltop Farm, Inc., a Maryland corporation with its principal place of business at
More information1. BREEDER: Equine Breeding Agreement
Equine Breeding Agreement This Equine Breeding Agreement (the Agreement ) is entered into as of by and between Hilltop Farm, Inc., a Maryland corporation with its principal place of business at 1089 Nesbitt
More informationCSU/ERL EMBRYO TRANSFER AGREEMENT 2018
Today s date: CSU/ERL EMBRYO TRANSFER AGREEMENT 2018 **Please include a copy of the mares registration papers when returning this contact MARE INFORMATION Registered Name: Breed: Registration No: Barn
More informationThis contract made by and between Watermark Farm ( The Rawles ) and
This contract made by and between Watermark Farm ( The Rawles ) and for breeding of the Mare named: Registration No: (Purchaser) to the Stallion named Showtime WF for the 2014 breeding season ( Present
More informationStallion Breeding Contract
Stallion Breeding Contract Fresh and/or Frozen Semen Breeding Agreement for the Hanoverian Stallion Waterford This Agreement is made this day of, by and between Lynn McEnespy of P.O. Box 773, Chico, Calif.
More informationCSU/ERL MARE BREEDING AGREEMENT 2018
CSU/ERL MARE BREEDING AGREEMENT 2018 Today s date: **Please include a copy of the mares registration papers when returning this contact MARE INFORMATION Registered Name: Breed: Registration No: Barn Name:
More informationTERMS AND CONDITIONS
Frozen Semen Breeding Contract, Paul Schockemoehle Hengsthaltung GmbH and Yancey Farms U.S. Contract is for the 2019 Breeding Season TERMS AND CONDITIONS Mare Owner Name: Phone: Address: Email:, hereby
More information2) Should the mare booked herein fail to conceive and carry a pregnancy the first season, substitution of another mare in her place may be allowed at
.BREEDING CONTRACT for frozen semen (for USA and Canada only!!) @ Team Nijhof, Kulsdom 9, Geesteren, Holland, all rights reserved. Subject to the following terms and conditions, I the undersigned (hereafter
More informationSTANDING STALLIONS TO THE PUBLIC
STANDING STALLIONS TO THE PUBLIC There are multiple measures of successfully standing a stallion to the public. The ultimate measure of success is progeny of the stallion that perform well in the arena
More informationSTALLION COOLED SEMEN CONTRACT
Page 1 Glenview Farm LLC Glenn Bouvier 6574 Dover Rd Shelbyville, KY 40065 (502) 647-0298 Alliance Stud LLC 1630 Todds Point Rd Simpsonville, KY 40067 (502) 722-0203 office (502) 817-5956 Linda Bennett
More informationEquine Practice Management
Equine Practice Management Power erful Softw twar are For The Equine Industr try Equine Reproduction Module Introducing the NEW The Equine Reproduction Module provides equine breeding facilities and equine
More informationMare owner understands that each shipment of semen will contain one dose of semen. A second
Date 477 Breeding Contract for 2018 Friesian Connection Stallion Station Inc. 2685 144 th Avenue Dorr Michigan 49323 Amy Kroll and Janice VerMerris, Stallion Managers Amy s Cell: 616-893-2964 Janice s
More informationFeeding the Broodmare
Reviewed August 2009 Agdex 460/50-6 Feeding the Broodmare Mares are expected to grow a healthy foal inside them for 11 months and produce enough milk for that foal for up to 6 months. Furthermore, many
More informationKALAMAZOO COUNTY 4-H HORSE PROJECT
KALAMAZOO COUNTY 4-H HORSE PROJECT Each year 4-H Members may choose to do either: 1. A notebook/ poster or tri-fold including all of the requirements for each appropriate year. (may be organized by topic
More informationCONTRACT FOR BREEDING
Reigate Cleveland Bay & Sporthorse Stud Michelle Williams PO Box 62, Dartmoor VICTORIA 3304 Mobile: 0412 385 274 Email: reigatestud@gmail.com Website: www.reigatestud.com CONTRACT FOR BREEDING Ad Design:
More informationIn the event that the mare dies, Mare Owner shall provide the Stallion Station with a veterinarian s certificate of death.
Tymen 503 2019 Breeding Contract for Frozen Semen Friesian Connection Stallion Station Inc. 2685 144 th Avenue Dorr Michigan 49323 Amy Kroll and Janice VerMerris, Stallion Managers Amy s Cell: 616-893-2964
More information4) No semen will be shipped without mare owner providing us with a signed contract and payment in full.
Julius 486 Sport Breeding Contract for Frozen Semen for 2018 Friesian Connection Stallion Station Inc. 2685 144 th Avenue Dorr Michigan 49323 Amy Kroll and Janice VerMerris, Stallion Managers Amy s Cell:
More informationBRITISH BREEDERS DINNER & AWARDS CEREMONY
BRITISH BREEDERS DINNER & AWARDS CEREMONY Saturday 14th January 2017 The Grange City Hotel AUCTION ITEMS Craig Payne - Photoshoot & digital portfolio of images Craig visits your home or yard at a mutually
More informationLive Foal Guarantee Breeding Contract for:
Live Foal Guarantee Breeding Contract for: (Frozen Semen) U.S. and Canada Contract is for the 2017 Breeding Season TERMS AND CONDITIONS Mare Owner Name: Phone: Address: Email:, hereby agrees to breed the
More informationGroup Project. Horse Leader:
Group Project. Names, ages, years in project: Table of Content: Excellent Good Incomplete Comments: Personal Story: Excellent Good Incomplete Comments: 1. Topic: Presented in a clean and professional manner
More informationMontana Rancher Record Notebook
Montana Rancher Record Notebook Megan Van Emon, Extension Beef Cattle Specialist Emily Glunk, Extension Forage Specialist This notebook is modified for Montana ranchers from the Florida Beef Cattle Ranch
More informationSTALLION COOLED SEMEN CONTRACT
P a g e 1 This form/contract certifies that STALLION COOLED SEMEN CONTRACT 1630 Todd s Point Road Simpsonville, KY 40067 502-722-0203 office / 502-817-5956 cell alliancestud@aol.com, herein referred to
More information28 NOVEMBER NOVEMBRE 2009
www.ivis.org BELGIAN EQUINE PRACTITIONERS SOCIETY (BEPS) XXVI de XXVI ème 28 NOVEMBER 2009-28 NOVEMBRE 2009 Met de medewerking van Avec la collaboration de Auditorium BMW Auditoire BMW UZ LEUVEN CHU Leuven
More informationBREEDING CONTRACT FROZEN SEMEN
Foothills Farm of Sedro-Woolley LLC Breeding Manager/Stallion Agent Kari Klingenberg 360-202-9008 BREEDING CONTRACT FROZEN SEMEN This contract dated this day of, 20 is between herein referred to as Mare
More information2011 BREEDING CONTRACT Sea Accounts
2011 BREEDING CONTRACT Sea Accounts This contract is made by and between Cynthia and Christopher DeBracy dba DeBracy Sport Horses (hereinafter DSH ) owners of the stallion SEA ACCOUNTS, and, (hereinafter
More informationWindswept Stables, LLC Empire s Power 2010 Contract 5033 John Wayland Hwy EVA Negative & Annual Vaccine
Windswept Stables, LLC Empire s Power 2010 Contract 5033 John Wayland Hwy EVA Negative & Annual Vaccine Dayton, VA 22821 AI only (540)820-2509 (540)879-5055 fax Please sign and complete. Include a copy
More informationEquine Science & Technology. Unit Title: Horse Reproduction and Parturition CIP:
Equine Science & Technology Unit Title: Horse Reproduction and Parturition CIP: 020221-09 Instructor: Objectives: After completing this unit of instruction, students will be able to: A. Describe the physiology
More informationHigher National Unit specification. General information for centres. Unit title: Equine Studies: The Brood Mare. Unit code: DP24 34
Higher National Unit specification General information for centres Unit code: DP24 34 Unit purpose: This Unit is designed to enable candidates to develop knowledge and utilise skills in the care and management
More informationScheckel Paint & Quarter Horses
Date Scheckel Paint & Quarter Horses STALLION SERVICE CONTRACT This contract certifies that a breeding exists for the 2012 season, between Scheckel Paint & Quarter Horses (Stallion Owner), Iowa State University
More informationSTALLION SERVICES AGREEMENT
STALLION SERVICES AGREEMENT This agreement for services is made and entered into as of 2014, between No Limit Farm and, the mare owner or lessee (hereafter referred to as Mare Owner ). 1. Services. Upon
More informationMare Breeding / Foaling Agreement 2018 Breeding Season. Mare Information. Owner Information
opline quine eterinary are Jennifer Teigen, D M 3087 130 th Ave Glenwood ity, WI 54013 (715) 684-9442 Mare Breeding / Foaling Agreement 2018 Breeding Season Today s Date: ** Please include a copy of the
More informationOsceola County 4-H Record Book Horse Intermediate (10-14)
Osceola County 4-H Record Book Horse Intermediate (10-14) Place Picture Here Name: Birthdate: Fair Age (as of Jan. 1) Address: Club: Years in 4-H: Years in Project: Member s Signature: Parent s Signature:
More informationEmpire's Power 2012 Contract EVA Negative & Annual Vaccine AI only
Windswept Stables, LLC 5033 John Wayland Hwy Dayton, VA 22821 (540)820-2509 (540)879-5055 fax Empire's Power 2012 Contract EVA Negative & Annual Vaccine AI only Please sign and complete. Include a copy
More informationDirections for completing contract
CHILLED SEMEN CONTRACT Directions for completing contract 1. Please print two copies of the contract or request two copies, read thoroughly, sign both and return to us. We will in turn sign both contracts
More informationSTALLION SERVICES AGREEMENT. 1. Mare Owner may be contacted at the following location: Street Address: City: State: Zip Code: Home Phone: Work Phone:
STALLION SERVICES AGREEMENT This Agreement entered into this day of, 2018, by and between Avalon Equine, hereinafter referred to as Stallion Owner &, hereinafter referred to as Mare Owner. In consideration
More informationOsceola County 4-H Record Book Horse Senior (15-19)
Osceola County 4-H Record Book Horse Senior (15-19) Place Picture Here Name: Birthdate: Fair Age (as of Jan. 1) Address: Club: Years in 4-H: Years in Project: Member s Signature: Parent s Signature: Leader
More informationDirections for Completing Contract
FROZEN SEMEN CONTRACT Directions for Completing Contract 1. Please print two copies of the contract or request two copies, read thoroughly, sign both and return to us. We will in turn sign both contracts
More informationRiverside Ranch Cindy McCraw
Riverside Ranch Cindy McCraw 34515 140 th St SE Sultan Washington USA 98294 Ph: 817-304-0800 Email: riversideranchtexas@gmail.com Frozen Semen Only Breeding Contract MACHINE MADE Owned by Dr. Cory Seebach
More informationKATE S RIDING CENTRE LTD STALLION SERVICE CONTRACT FOR LIVE COVER AND ARTIFICIAL INSEMINATION
KATE S RIDING CENTRE LTD STALLION SERVICE CONTRACT FOR LIVE COVER AND ARTIFICIAL INSEMINATION Between KATE S RIDING CENTRE LTD and Name: Address: Phone: Mobile: Email: THIS IS TO CERTIFY A BOOKING FOR:
More informationBull management. Growing bulls 116. Selecting the best bulls to use 117. Managing working bulls 118. Assessing bull performance 120
12 Growing bulls 116 Selecting the best bulls to use 117 Can I skimp on feed for the bulls until the break comes? 117 Managing working bulls 118 Sometimes bulls can be really hard to handle on the farm.
More informationCoquat Ranch 2016 Cooled Shipped Semen Breeding Contract
Coquat Ranch 2016 Cooled Shipped Semen Breeding Contract Owner of Mare Owner of Stallion Name: Sarah Coquat Address: 5454 CR 4491 Address: Aransas Pass, Texas 78336 Phone: (361) 319-2733 Email: sarahmc09@yahoo.com
More informationDate in effect: 7/13/2016. Page: 1 of 5
Author: Jennifer Brazil 1 of 5 Responsible faculty: (Signature/Date) PERSONNEL Phil Smith Training All personnel will be trained via IACUC and TTEC training in 1. Husbandry needs 2. Handling and restraint
More informationBREEDING AGREEMENT THEREFORE, THE PARTIES AGREE AS FOLLOWS: 1. RECITALS. The above Recitals are made a part of this Agreement.
BREEDING AGREEMENT THIS AGREEMENT is made and entered into on this day of 20, by and between WILD TURKEY FARM (hereinafter referred to as WT ) and located at (hereinafter referred to as Owner ). RECITALS:
More informationTHE PITTMAN RANCH 1693 HIGHWAY 277, CHIPLEY, FLORIDA (850) (727) FAX (850) VS CODE BLUE 2018 BREEDING CONTRACT
THE PITTMAN RANCH 1693 HIGHWAY 277, CHIPLEY, FLORIDA 32428 (850) 774 4477. (727) 207-6384 FAX (850) 638-9887 VS CODE BLUE 2018 BREEDING CONTRACT This agreement, made on the date last shown below, by and
More informationShenandoah Gypsy Vanner Horses, LLC. Stallion Service Agreement
Shenandoah Gypsy Vanner Horses, LLC. Stallion Service Agreement This contract is for the breeding season of 2017 between Shenandoah Gypsy Vanner Horses, LLC. (stallion owner, and hereinafter referred to
More informationHORSE. Program. Kentucky 4-H. Achievement. Level 4. 4AF-04PO
4AF-04PO Kentucky 4-H HORSE Achievement Program Level 4. University of Kentucky College of Agriculture Cooperative Extension Service Agriculture Home Economics 4-H Development Congratulations on arriving
More informationProf. Dr. Alexandre Augusto de Oliveira Gobesso Departamento de Produção e Nutrição Animal Faculdade de Medicina Veterinária e Zootecnia Universidade
Prof. Dr. Alexandre Augusto de Oliveira Gobesso Departamento de Produção e Nutrição Animal Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo - Brasil (55) 19 3565 4222 Gobesso.fmvz@usp.br
More informationAttachment 2. HERD HEALTH REVIEW OF EQUINE RANCHES Review 1 Date This Herd Heath Review covers the period from March 1, 2013 February 28, 2014
HERD HEALTH REVIEW OF EQUINE RANCHES Review 1 Date This Herd Heath Review covers the period from March 1, 2013 February 28, 2014 Contract Name Contract Number Veterinarian (Name) Veterinarian Phone Number
More informationDay 1 Worksheet Foaling History and Due Date Calculation
Day 1 Worksheet Foaling History and Due Date Calculation Foaling History One of the most useful tools you can have in your belt when it comes to foaling a mare is knowledge of her foaling history. Each
More informationValleyfield Farm. Ellicia Edgar rr14 Parkland County, ABT7Y 2N2 (780) (780)
Breeding Contract Valleyfield Farm Ellicia Edgar 50515 rr14 Parkland County, ABT7Y 2N2 (780)994-9887 (780) 968-3925 valleyfieldfarm@icloud.com This contract is made (day/month) of 2019 between Valleyfield
More informationRoyal Vista Southwest PO Box 673 Purcell, OK Dear Client,
Royal Vista Southwest PO Box 673 Purcell, OK 73080 Dear Client, I have enclosed the Embryo Transfer contract(s) for the 2011 season. Please complete, sign and return together with the $1000 Booking Fee.
More informationRoyal Vista Southwest th Street Purcell, OK Dear Client,
Royal Vista Southwest 26213 170 th Street Purcell, OK 73080 Dear Client, I have enclosed the Embryo Transfer contract(s) for the 2019 season. Please complete, sign and return together with the $1000 Annual
More informationRoyal Vista Southwest th Street Purcell, OK Dear Client,
Royal Vista Southwest 26213 170 th Street Purcell, OK 73080 Dear Client, I have enclosed the Embryo Transfer contract(s) for the 2019 season. Please complete, sign and return together with the $1000 Annual
More informationLevel 2. University of Kentucky College of Agriculture Cooperative Extension Service Agriculture Home Economics 4-H Development
Level 2. University of Kentucky College of Agriculture Cooperative Extension Service Agriculture Home Economics 4-H Development Welcome to Level 2 of your 4-H Horse Achievement Program. You have successfully
More informationRebedon Farm. Rebecca McGregor STALLION CONTRACT GENERAL TERMS
Rebedon Farm Rebecca McGregor 43 Colby Place, Phillipsburg NJ 08865 610-905-2851 beckypbb@hotmail.com STALLION CONTRACT GENERAL TERMS This contract, for the breeding season of 2012, is made and entered
More informationBroodmares represent a large financial and time
A l a b a m a A & M a n d A u b u r n U n i v e r s i t i e s Broodmare Management ANR-1306 Broodmares represent a large financial and time commitment, even when the mare conceives quickly and maintains
More information2018 BREEDING CONTRACT
Dated.../.../... 2018 BREEDING CONTRACT This contract certifies that, (herein referred to as the Mare Owner/s), has entered into this contract with Trish Wettenhall t/a Triandibo Quarter Horse Stud (herein
More informationBIG STAR 2019 Frozen Semen Breeding Contract
BIG STAR 2019 Frozen Semen Breeding Contract This agreement confirms the nomination to the stallion BIG STAR (hereinafter referred to as the Stallion ) for the 2018 breeding season by the owner of lessee
More informationHORSE PRODUCTION BREEDING RECORD
C0858E HORSE PRODUCTION BREEDING RECORD Add this sheet to your regular 4-H Record Book. Keep all your records in one book. Project enrollment number Years in 4-H* Years in Horse Project* Years in Breeding
More informationPASTURES FOR HORSES: CHALLENGES AND OPPORTUNITIES
PASTURES FOR HORSES: CHALLENGES AND OPPORTUNITIES Bob Coleman Department of Animal and Food Sciences University of Kentucky Horses were born to eat grass and how they evolved has allowed them to be efficient
More informationCOMPARISON OF FIXED & VARIABLE RATES (25 YEARS) CHARTERED BANK ADMINISTERED INTEREST RATES - PRIME BUSINESS*
COMPARISON OF FIXED & VARIABLE RATES (25 YEARS) Fixed Rates Variable Rates FIXED RATES OF THE PAST 25 YEARS AVERAGE RESIDENTIAL MORTGAGE LENDING RATE - 5 YEAR* (Per cent) Year Jan Feb Mar Apr May Jun Jul
More informationBreeding Contract. This contract is made (day/month) of 2012 between Dreamscape Farm and. , Purchaser, for the breeding of the Mare,
Dreamscape Farm Jennifer and Armin Arnoldt 7307 Telegraph Trail, Langley, BC V1M-2M2, Canada 604-881-6038 (Phone) 604 881-6093(Fax) dreamscapefarm@shaw.ca Breeding Contract This contract is made (day/month)
More information2018 BOARDING AND BREEDING SERVICES CONTRACT
Premier Breeding Services, LLC PO Box 660, Kiowa, CO 80117 Tel: 719-240-1598 Brad Ray, M.S., Owner/Manager E-mail: eqreproman@aol.com THIS BOARDING AND BREEDING SERVICES CONTRACT is made and entered into
More informationFLORIDA 4-H. Horse Project Record Book 4H HSR 02. for use by all Southwest Florida and Lee County Fair Horse Exhibitors Years in Club Work
4H HSR 02 FLORIDA 4-H Horse Project Record Book for use by all Southwest Florida and Lee County Fair Horse Exhibitors 2019 Name Address County Date of Birth Years in Club Work Name of Club Leader # of
More informationUnsure of exact breeding dates. Minimal labour for the breeder. Stallion may focus on a favourite mare/ignore Extra handlers not required.
MHCO Spring Clinic 2005 Reproduction for the Miniature Horse By: Michelle Courtemanche D.V.M. The following handout is based on information from reputable sources as well as the experience and opinions
More informationHastening the onset of the breeding season. by Julie Skaife, of Select Breeders Services
Hastening the onset of the breeding season. by Julie Skaife, of Select Breeders Services The horse is a seasonal breeder meaning that natural mating occurs during certain times of the year to ensure that
More informationR & R KNUDSEN STUD HORSE BREEDING CONTRACT THIS AGREEMENT is made between the party named in Item 1 of Schedule 1 (Stud) and the party named in Item
R & R KNUDSEN STUD HORSE BREEDING CONTRACT THIS AGREEMENT is made between the party named in Item 1 of Schedule 1 (Stud) and the party named in Item 2 of Schedule 1 (Mare Owner). BACKGROUND: 1. The Stud
More informationMare Owner: Address: City: State: Zip Code: Telephone #: Alternate #: Address: Mare Name: Registration #: Registry: CAHR Other:
This agreement is made this day of, 2018, by and between Gretchen M. Love --Stallion Owner; Abraham s Equine Clinic (AEC) Stallion Manager; Strand s Arabian Stables (SAS) Agent/Trainer; and: Mare Owner:
More information2017 Cooled Semen Breeding Contract United States
This certifies, hereafter referred to as MARE OWNER, has engaged in one service to The Big Sensation, APHA Registration #742,822, for the mare, Breed, Registration # for the 2017 breeding season. BREEDING
More informationFoaling Schedule and Newborn Foal Guideline 2003 by Dr. Robert L. Leonard, DVM, AAEP at
Foaling Schedule and Newborn Foal Guideline 2003 by Dr. Robert L. Leonard, DVM, AAEP at www.horsedoc.org I. Prenatal: Preparations before and leading up to foaling A. 2 months in advance... 1. remove mares
More information**Stallion Service Contract** Presidential Order
This agreement is made this day of, 2018, by and between Abraham s Equine Clinic (AEC), and: Mare Owner: Address: City: State: Zip Code: Telephone #: Alternate #: Mare Name: Registration #: Registry: AQHA
More information1. ENGAGEMENT OF SERVICE. Mare Owner hereby engages one service by shipped. Registration No.: Breed:
1 THE PITTMAN RANCH 1693 HIGHWAY 277, CHIPLEY, FLORIDA 32428 (850) 774 4477 JAN PITTMAN FAX (850) 638-9887 VS CODE BLUE 2019 CONGRESS SPECIAL-$1500.00 Stud Fee only RETURNING CUSTOMER will receive additional
More informationFeeding the Broodmare.
Feeding the Broodmare. This subject is enough for a book in its own right and could cover everything from avoiding OCD to managing the overweight mare at weaning. Here we will stick to basics and try to
More information2016 / 17 BREEDING CONTRACT
Dated.../.../... 2016 / 17 BREEDING CONTRACT This contract certifies that, (herein referred to as the Mare Owner/s), has entered into this contract with Trish Wettenhall t/a Triandibo Quarter Horse Stud
More informationBRUNSON EQUESTRIAN, LLC
14078 State Hwy 110N, Tyler, Texas 75704 (903) 882-8696 Fax (903) 881-0228 www.texasrosehorsepark.com WELCOME TO BRUNSON EQUESTRIAN, LLC Brunson Equestrian, LLC provides riding lessons in the disciplines
More informationVARIAN ARABIANS Farm Text STALLION SERVICE CONTRACT FOR TRANSPORTED SEMEN
VARIAN ARABIANS Farm- 805-489-5802 Text 805-441-1229 Email angelaalv@aol.com STALLION SERVICE CONTRACT FOR TRANSPORTED SEMEN Stallion Name: Registration # Mare Name: Registration # Mare s date of birth:
More informationWhoever said a horse. The Key to the Future: Broodmare and Stallion Care
The Key to the Future: Broodmare and Stallion Care While they only make up a small segment of the equine industry, breeding stock is the future of our business Bryant Craig, DVM Whoever said a horse is
More informationClose this window to return to IVIS
www.ivis.org Management of Dystocia Michelle M LeBlanc Rood and Riddle Equine Hospital Lexington, KY Approach a dystocia with a consistent plan aimed at delivering the foal in the shortest possible time
More informationFEEDING THE PREGANT MARE
FEEDING THE PREGANT MARE So you just received the results of the 14 day ultra sound and your mare is in foal; congratulations, you have successfully negotiated the first hurdle. Now for the long wait.
More informationAbrahams Equine Clinic
Abrahams Equine Clinic 2013 Shipped Semen Contract Mare owner is responsible for returning the shipping container to AEC within 5 days of receiving.this agreement is made this day of, 2012/2013, by and
More informationA TOUCH OF SUDDEN Stallion Service Contract
This agreement is made this day of,, by and between (Sherri Banta) Stallion Owner; Abraham s Equine Clinic (AEC); and: Mare Owner: Address: City: State: Zip Code: Telephone #: Alternate #: Mare Name: Registration
More informationBREEDING CONTRACT Stallion Service for Transported Cooled Semen
BREEDING CONTRACT Stallion Service for Transported Cooled Semen Stallion name: IXL Noble Express Registration #: Mare name: Registration #: Mare date of birth: Breed: Email: Today s Date: 538771 THIS BREEDING
More informationBREEDING AGREEMENT (New Zealand)
BREEDING AGREEMENT (New Zealand) THIS AGREEMENT is made and entered into on this day of, 200, by and between WILD TURKEY FARM LLC a Oregon Limited Liability Company (hereinafter referred to as WT and,
More informationGK Performance Horses, LLC Fresh Cooled Semen Contract 2018 Breeding Season
GK Performance Horses, LLC Fresh Cooled Semen Contract 2018 Breeding Season MARE OWNER Name: Address: City: State: Zip: Phone: Email: DESCRIPTION OF MARE Name: Age: Breed: Registration: Sire: Breed: Dam:
More information2018 Calf Challenge Record Book
2018 Calf Challenge Record Book Exhibitor: State: County: IMPORTANT DATES DATE November 18, 2017 January 1, 2018 March 1, 2018 May 1, 2018 July 1, 2018 August 31, 2018 September 1, 2018 September 27-30,
More informationBREEDING CONTRACT FOR FROZEN SEMEN
BREEDING CONTRACT FOR FROZEN SEMEN This agreement confirms the reservation to the stallion (hereinafter referred to as the Stallion ) for the 2015 breeding season by the owner or lessee of the mare whose
More informationThank you for attending and we look forward to hearing of your breeding successes in the future!
INTRODUCTION 2 We would like to take this opportunity to welcome you to our Equine Reproduction Short Course. As breeders ourselves, we appreciate the difficulties that can be faced when trying to run
More information2. This horse breed has two different color patterns: tobiano and overo. a. Appaloosa b. American Paint c. Paso Fino d. American Quarter Horse
2015 State FFA Horse Evaluation and Selection CDE Iowa FFA Association FFA Enrichment Center 1055 SW Prairie Trail Parkway Ankeny, IA 50023 Please put all answers on the Answer Sheet 1. If a horse measured
More informationGoat Care and Maintenance Goat Care and Maintenance
Goat Care and Maintenance Goat Care and Maintenance Goats have to be one of the most entertaining farm animal to own. Knowing how to perform the required goat care is the most important first step to take,
More information