Bioreactor System ERT 314. Sidang /2011
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1 Bioreactor System ERT 314 Sidang /2011
2 Chapter 2:Types of Bioreactors Week 4
3 Flow Patterns in Agitated Tanks The flow pattern in an agitated tank depends on the impeller design, the properties of the fluid, and the size and geometric proportions of the vessel, baffles and agitator In circular flow, liquid moves in a streamline fashion and there is little mixing between fluid at different heights in the tank
4 Circular flow in an unbaffled stirred tank viewed from above (a) Vortex formation during circular flow (b)
5 Radial-flow impellers These impellers have blades which are parallel to the vertical axis of the stirrer shaft and tank such as the six-flat-blade disc turbine (Rushton turbine) Liquid is driven radially from the impeller against the walls of the tank where it divides into two streams, one flowing up to the top of the tank and the other flowing down to the bottom These streams eventually reach the central axis of the tank and are draw back to the impeller Radial flow impellers also set up circular flow which must be reduced by baffles
6 Flow pattern produced by a radial flow impeller in a baffled tank, (a) side view; (b) bottom view
7 Axial-flow impellers These impellers have blades which make an angle of less than 90 to the plane of rotation and promote axial top-to-bottom motion such as propellers pinched blade turbine Fluid leaving the impeller is driven downwards until it is deflected from the floor of the vessel Then, it spreads out over the floor and flows up along the wall before being drawn back to the impeller The impellers are useful when strong vertical currents are required i.e if the fluid contain solids, strong axial flow of liquid leaving will discourage settling at the bottom of the tank
8 Pinched blade turbine The angle is <90
9 Flow pattern produced by a axial flow impeller in a baffled tank, (a) side view; (b) bottom view
10 Mechanism of Mixing For mixing to be effective, fluid circulated by the impeller must sweep the entire vessel in reasonable time The velocity of fluid leaving the impeller must be sufficient to carry material into the most remote parts of the tank which development of turbulence is also necessary to ensure better mixing Three physical processes are among important factors in mixing: distribution dispersion diffusion
11 Mechanism of Mixing (cont d) Distribution is called macromixing, diffusion is called micromixing whereas dispersion can be classified as either micro or macromixing depending on the scale of fluid motion In large vessels, the size of circulation paths is large and the time taken to tranverse them is long, therefore distribution is often the slowest step in mixing process If the rotational speed of the impeller is high, distribution step become turbulence where fluid no longer travel in streamlines but move erratically in form of cross-current
12 The pattern of bulk fluid flow in vessel stirred by a radial-flow impeller as shown in the figure High turbulence region is developed near the impeller where current converge and exchange materials The flow become slower and large streamline is developed as current move away from the impeller which mixing in these region is less intense than near the impeller Flow pattern developed by centrally-positioned radial-flow impeller
13 Mechanism of Mixing (cont d) The process of breaking up bulk flow into smaller eddies is called dispersion, where it facilitates rapid transfer of material The degree of homogeneity of the broth by dispersion is limited by the size of smallest eddies formed in particular fluid The size of eddies can be measured by Kolmogorov scale of turbulence, λ : where: 3 v λ = characteristic dimension of smallest eddies V = kinematic viscosity ε= rate of turbulence energy per unit mass of fluid 1 4
14 Mechanism of Mixing (cont d) According to the equation (previous slide), the greater the power input (ε) to the fluid, the smaller are the eddies Smaller eddies also are produced by low viscosity of fluid (v), i.e water have λ in the range of μm, which the smallest scale of mixing can be achieved by dispersion
15 Mechanism of Mixing (cont d) To achieve mixing on a scale smaller than Kolmogorov scale, diffusion can be applied Molecular diffusion is generally regarded as slow process, but if in small distances it can be accomplished quite rapidly i.e for λ of μm, homogeneity can achieved in 1 second for low viscosity fluids
16 Power Requirements for Mixing For a given stirrer speed, the power required depends on the resistance offered by the fluid to rotation of the impeller Average power consumption per unit volume for industrial bioreactors Small vessels 10 kwm-3 Large vessels 1 2kWm-3 Friction in the stirrer motor gearbox and seals reduce the energy transmitted to the fluid, therefore the electrical power consumed by the stirrer motor is higher than the mixing power by an amount depending on the efficiency of the drive
17 Ungassed Newtonian Fluids Mixing power for non aerated fluid depends on: the stirrer speed the impeller diameter and geometry the properties of the fluid (i.e density and viscosity) The relationship of these variables is expressed in terms of dimensionless numbers such as Reynolds number, Re and power number, Np
18 Ungassed Newtonian Fluids (cont d) Power number, Np: Once value of Np is known, the power required is calculated by: P N P N P N N P 3 i D 3 i 5 i D where: P = power; ρ = fluid density; N = stirrer speed, D = impeller diameter 5 i
19
20
21 Ungassed Newtonian Fluids (cont d) Laminar regime: Corresponds to Re<10 for many impellers such as anchor and helical ribbon, which laminar flow persist until Re = 100 or greater. N P 1 Re i or P k 1 N Power required for laminar flow is independent of the density but directly proportional to viscosity 2 i D 3 i
22 Ungassed Newtonian Fluids (cont d) Tubulence regime: Power number is independent of Re number in turbulent flow P N ' P N N p for turbines is significantly higher than for most other impellers, indicating turbine transmit more power to the fluid than other design Power required for turbulent flow is independent of the viscosity but proportional to fluid density 3 i D 5 i
23 Constants Impeller type K1 (Re = 1) N p (Re = 10^5) Rushton turbine Paddle 35 2 Marine propeller Anchor Helical Ribbon
24 Ungassed Newtonian Fluids (cont d) Transition regime: Between laminar and turbulent flow regime. Both density and viscosity affect the power requirement in this regime Usually a gradual transition from laminar to fully developed turbulent flow in stirred tanks
25 Gassed fluids Liquids into which gas is sparged have reduced power requirement Gas bubbles decrease the density of the fluid but the influence of density on power requirement does not adequately explain all power characteristic of gas-liquid phase The presence of bubble also affect the hydrodynamic behaviour of fluid around the impeller Ratio gassed to ungassed power: where: P P g F N V i g 0.25 N 2 i D gwv i 4 i 2 / Pg = power with sparging; P0 = power without sparging; Fg = volumetric gas flow rate; N = stirrer speed, V = liquid volume; D = impeller diameter; g =gravity; W =impeller width
26 Types of Sparger Large variety of sparger designs: Simple open pipes Perforated tubes Porous diffusers Complex two-phase injector
27 Types of Sparger (cont d) Porous diffuser Perforated tubes Open pipes
28 Types of Sparger (cont d)
29 Flow pattern in stirred aerated bioreactors When air is sparged different gas flow patterns develop depending on the relative rates of gas input and stirring If the agitator speed Ni is low and gas feed rate Fg is high, gas envelopes the impeller without dispersion and the flow pattern dominated by air flow up the stirrer shaft Impeller flooding is occur which the gas handling capacity of the stirrer is smaller than the amount introduced The flooding should be avoided because an impeller surrounded by gas no longer contacts the liquid properly resulting poor mixing and gas dispersion
30 Flow pattern in stirred aerated bioreactors as a function of impeller speed, N and gas flow rate, Fg
31 Flow pattern in stirred aerated bioreactors (cont d) As the impeller speed increases, gas is captured behind the agitator blades and is dispersed into liquid NiB is the minimum stirrer speed required to just completely disperse the gas, including below the impeller The minimum agitator tip speed for dispersion of air bubbles is about ms-1 Further increase in stirrer speed, small recirculation patterns start to emerge which NiR is the speed at which gross recirculation of gas back to the agitator starts to occur
32 Flow pattern in stirred aerated bioreactors (cont d) Under typical fermenter operating conditions, increasing the stirrer speed improves the value of kla In contrast, except in very low sparging rate, increasing the gas flow is generally considered to exert only a minor influence on kla Increasing number of impeller on the stirrer shaft does not necessarily improved kla even though the power consumption is increased The quantity of gas passing through the upper impellers is small compared with the lower impeller, so that any additional gas dispersion is not significant
33 Dependence of kla on stirrer speed Ni in agitated tank with superficial gas velocity Effect on kla of number of impeller used to mix a viscous mycelial suspension
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