NMR FACILITY REGISTRATION FORM

Transcription

1 NMR FACILITY REGISTRATION FORM Have you registered on-line? Y / N (if NO, please visit this link and follow step 1. *First name: Middle initial: *Surname: *Student / Staff ID Number: * *Type of Research: (e.g.: Hons Student, PostDoc): Access to NMR (B41) will begin at the completion of the NMR Induction and Registration, however you MUST indicated a date you will no longer require access to NMR (B41) *Expire : / /20 (D/M/Y) *Supervisor s Name: *Send NMR bill to (Name): (and fill in account details below) *Project Title: Have you used NMR before? Y / N (if yes WHERE and WHEN) NMR usage required for (consult supervisor and please circle): 300MHz (AutoSampler- GYRO solution NMR) Training is offered on day of NMR Induction One-one training must be organised with certified NMR Staff if you require usage for the below instruments. EPR 300MHz (solution NMR) 300MHz / 700MHz (solid state NMR) 600MHz (solution NMR) 400MHz (solution NMR) 500MHz (solution NMR) 600MHz (solution Cyroprobe NMR) *All users must provide the following account information (consult supervisor): Fund Dept. ID Project No Your signature: *Supervisor s signature: Date: Date: *: Indicating the compulsory data fields # PICTURE NUMBER: Office Use Only GYRO: OBS: SWIPE CARD ACCESS: SEND CONFORMATION TO USER: OHS INDUCTION: MICRO ACCESS: USERS ALIAS: # USER CODE: 1 Nov 2014

2 SAFETY IN THE NUCLEAR MAGNETIC RESONANCE (NMR) FACILITY NMR instruments use a static magnetic field to make nuclear spins orient in the magnetic field. There are different strengths of magnets from 1,500 to 200,000 gauss (0.15 to 21 tesla). Field strengths correlate with the proton frequency of the instruments here at the UNSW NMR Facility: 1 H Frequency (MHz) Field (Tesla, T) Horizontal distance of 5 gauss line (in metres) from centre of the magnet * Bruker Bruker Bruker *vertical distances are larger These are the strengths of fields at the centre of the magnet. Fields at the extremities of the magnet casing do not exceed 2 tesla. No permanent biological effects have been measured in humans below this field strength. The magnetic field drops rapidly with increasing distance from the centre of the magnet and an important measurement is the distance from the centre of the magnet at which the field drops to 5 gauss, which is the cut off point for most safety issues. The main safety concerns are: 1) The large magnetic field which attract ferromagnetic objects, and 2) The cryogens may cause burns as liquids when they contact with the skin or by asphyxiation when present in large concentrations produced by a quench or container failure. RISK ASSESSMENT FOR GENERAL USAGE Strong Magnetic Fields The magnet of a spectrometer is ALWAYS at field. Strong fields are produced outside the magnet; therefore, no movable metal objects should be taken within the ~2 to 5 metres (3 to 5 gauss) radius of the instrument. Small, sharp metal objects flying towards the magnet are highly dangerous. Larger objects are troublesome to scrape off the magnet, and can seriously damage the magnet. Very large objects have been known to break the cryogen dewar(s) in the magnets resulting in the release of the cryogens contained in the magnet (See Quench). Magnetic objects flying towards the magnet pose threat of serious injury/death to any person in the way. Metal belt buckles; steel tipped shoes, paper clips, hair pins, and any other metal on the person may be strongly attracted when close to the magnet. 2 Nov 2014

3 Persons fitted with pacemakers should not enter rooms containing spectrometers. Magnetic fields may affect heart pacemakers. Demand-type pacemakers may be switched to basic rate pacing. Persons fitted with metallic implants and prostheses should not get closer than the 5 gauss line of a magnet. Magnetic fields may permanently damage analogue watches, calculators and certain types of credit cards and mobile phones. Keep those items more than 2.5 m away from the centre of magnet. NMR workers should spend no longer than reasonable necessary within the 5 gauss line for sample changing and adjustments. Hands and arms etc. should not be placed into the bore of the magnet (widebore VI 300). Cryogenic Liquids In the unlikely event of the magnet quenching (the rapid release of gaseous cryogens from the cryostat into the room) or of a cryogenic container failure, up to 100 m 3 of helium gas may evolve over a period of several minutes. Although inert, lighter than air and non-toxic, there could be a risk of asphyxiation in a confined space. Personnel should evacuate the NMR laboratory IMMEDIATELY in such a situation. A quench warranting evacuation would be obvious by the noise of the escaping gas and clouds of vapour. Similarly, rupture of a liquid nitrogen container resulting in a large volume of nitrogen gas being released also requires evacuation of the laboratory. Failure to refill or de-energize the magnet when low levels are indicated by cryogen level sensors may result in a magnet quench with possible magnet damage. If low level warning lights on sensors are continuously lit and warning messages are displayed on the BSMS instrument panel, immediately inform the NMR facility staff. Electrical Hazards Electrical/RF hazards: Risks are similar to those encountered in the use/maintenance of other laboratory equipment. Changes to cables and wiring should only be made by NMR Facility staff. In case of serious flooding, or in other situations where there is risk of electrocution, the equipment circuit breakers will be turned off by NMR Facility staff. Sample Care Breakage and Accidents ALL accidents inside the probe (e.g. broken tubes) MUST be reported to NMR Facility staff IMMEDIATELY. Knowledge of any chemicals involved is crucial for clean-up. All spills/breakages 3 Nov 2014

4 outside the magnets should also be cleaned up using the appropriate methods outlined on the relevant MSDS. Solvent Exposure Samples in organic solvents should NOT be prepared in the spectrometer rooms. Use your own laboratories or the prep lab (B61). Labelling and Disposal of Samples All NMR samples MUST be labelled with owners name and an appropriate identifier to avoid safety issues of unknown compounds. All users MUST dispose of their own samples in their own laboratories. Short term storage of labelled NMR tubes in the spectrometer rooms (e.g. in the fridge) is permitted for samples that do not present significant safety risk. Fire Use only carbon dioxide fire extinguishers to avoid equipment damage. 4 Nov 2014

5 RISK ASSESSMENT FOR CRYOGEN FILLS When transferring liquid nitrogen or helium, the following steps should be observed to avoid accidents: Only personnel trained by Facility staff following official procedures may perform cryogen fills. Gloves, eye protection, and closed shoes (NOT STEEL CAPPED) must be worn. All doors should be propped open to increase ventilation. Only stainless steel transfer lines are to be used to avoid problems of cracking or magnetic attraction. Tanks on wheels must be chocked or held by another person or transported on purpose built stable trolleys. The transfer must be continuously attended. No access to the pit (DMX 600) is permitted during nitrogen fills as exit is hampered and the cold N 2 is denser than air. Since the possibility of a quench is higher when filling the magnet, and since the transfer involves manual operations, there is a remote possibility that an operator could be rendered unconscious around the time of a quench. Fills should only be done by a single operator when the fill cannot be deferred, and exceptional caution should then be used. QUENCH Currently, all superconducting magnets use liquid helium to maintain the magnet coils at a temperature enabling superconductivity. The helium and magnet coils are maintained in a vacuum. The temperature of liquid helium is approximately -269 degrees C or 4.17 degrees K. Liquid helium will boil at 4.22 degrees K. Any disruption to the temperature or loss of the vacuum will cause the liquid helium within the magnet to "boil off" causing an immediate and sudden loss of the magnetic field. This event is referred to as a "quench". When this occurs, the helium will expand at a rate of approximately 760 to 1. This means that for every litre of liquid helium, 760 litres of gas would be produced. This marked expansion in volume causes the gas to rush out of the magnet into the spectrometer room. Magnets in use hold more 100 litres of liquid helium and liquid nitrogen (DMX 600). 5 Nov 2014

6 NMR Induction Safety Quiz (Attempt before 11am Induction) 1) What does the 5-gauss line mean? 2) List three objects that should NOT pass the 5- gauss line? 3) What is a cryogen? Name the two liquid cryogens used in the magnets and their potential hazards. 4) What is a NMR quench? What should one do in an event of a NMR quench? 5) Lab coats, food, drinks and plastic/latex gloves should NOT enter the NMR Lab (B41). 6) Indicate the location of a) g) on the map below: a. 2 x Fire blankets b. 2 x Fire extinguishers c. 2 x Emergency Exits d. First aid box e. 1 x Broken glass bin f. telephone (in B41) g. 1 x UNSW Emergency Procedure clip chart (in B41). 7) Indicate (LHS) i) field strength in MHz (in A-I only) and ii) main function/s and iii) instrument s nickname. A) B) C) D) E) F) G) H) I) j) 6 Nov 2014

7 8) How to prepare a perfect NMR tube for analysis. (Answer the 5 questions below). 1. Why should you Parafilm your cap to your tube? (If submitting a sample on an auto-sampler) 3. How would you label your tube? (Illustrate this on the tube) What should you used to label the NMR tube? Maximum and minimum NMR tube lengths? (if submitting a sample on the auto-sampler Gyro) Max= cm Min= cm 5. How much deuterated solvent should be used in a 5mm NMR tube (if submitting a sample on an auto-sampler eg: GYRO)? cm, ml or ul 7 Nov 2014

8 9) These samples were prepared for the auto-sampler Gyro. What s wrong with these samples? Tube 1- Tube 2- Tube 3- Tube 4- Tube 5- Tube 6-24cm 18cm 4cm Nov 2014

9 Nuclear Magnetic Resonance OHS Induction Form- New User UNSW This form is to be completed by NMR staff in the company of users seeking NMR access. Your details: Name: Supervisor's name: School/ Unit: Requirements New users are made aware of: (tick when completed) Swipe card access to B41 (NMR) will be activated on completion of this induction and registration. Users may NOT lend their access card, nor BORROW other peoples access card. Users are NOT to bring friends or colleagues into the NMR lab without (a) prior arrangement with a NMR staff and (b) appropriate safety induction of the second person. The telephone in the NMR lab may be used in the event of an emergency. Users must make themselves aware of emergency contact details for security (Ext: 56666). The identity of the first aid officer and their contact number is located on the first aid box. If the Fire Alarm OR the Gas Panel Alarm are activated, EVERYONE MUST exit by the main door (B41) or from the computer room. Once you leave this facility make your way to the village green via the stairs. Samples are the responsibility of the users and the NMR Facility does not accept any responsibility for loss or damage of samples left in the facility User must NOT prepare samples in the NMR lab, NO chemicals are to be disposed of in this facility. The NMR facility does not have a chemical waste disposal system. If a NMR tube breaks in the lab inform a staff immediately. Data is the responsibility of the users and the NMR Facility does not accept responsibility for loss or damage of data. Users must follow the provided Safe Working Procedures when using an instrument. Users MUST be familiar with the NMR's Risk Assessment. Training for ALL instruments may only be provided by NMR staff. Users must NOT provide training to other users. NMR staff will train new users on the auto-sample and also explains the rules in place. Users must NOT wear a lab coat or plastic and or latex gloves in the NMR lab. Users must NOT bring food or drinks into the NMR lab. Users must NOT perform any functions or use any instruments which they have not been trained on. A user/ user s supervisor will be charged for instrument time if they fail to turn up for booked sessions. Users must cancel unwanted sessions with >24hrs notice and inform NMR staff. Users must NOT install software on any of the unit's computers or change any settings on any computer in the unit. Users must NOT download music, multimedia files or other files not directly related to their research work through any of the unit's computers. NO INTERNET access on any of the instrument computers. Any work that is published or publicly presented, where all or a part of the work was undertaken in the NMR Facility, should acknowledge the role of the UNSW NMR Facility in providing access or contributing to or assisting in the work. Access to this facility is a privilege which may be revoked if users do not operate instruments in the unit in a safe and responsible manner. Lhpsuyl tv cvtwsy wpto tol hivvl ylxupyltluts wpss ylsust pu yvuy TSR hcclss ilpun ylvplwlk. Signatures Your signature: Date: NMR staff signature: Date: 9 Nov 2014

10 After Hours Motion Sensor User s Guide If you are accessing the NMR Facility (B41) between 9pm-6am (M-F), on weekends or on public holidays, please follow the instructions below. The motion sensor system (also known as CARDAX) is an intruder alarm system located in the corridor opposite door B54. It is used to arm and disarm the alarm zones to which a user has access. The user navigates through the menus using the four soft keys under the display. The function of the keys varies according to the menu level. To begin: Swipe access card through reader mounted below keypad. Greeting Please Choose Action is displayed. Press the arm or disarm key according to your need. Arm to turn on the alarm when exiting the room. Disarm to turn off the alarm when entering the room. ARM Press arm. A list of alarm zones appears. Only zones that are currently disarmed or in your access group will appear in the list. A d on the right of the screen indicates disarmed state. Select the alarm zone to be armed using the keys on the keypad. Press OK. Attempting to Arm is displayed. Followed by Zones are armed. The screen returns to the arm list, the zone armed will no longer be displayed. Press back and then logout to finish. DISARM Press Disarm. A list of alarm zones appears. Only zones currently armed or in your access group will be displayed. An a on the right of screen indicates the armed state. Select the alarm zone to be disarmed using the keys on the keypad. Press OK. Zones are Disarmed is displayed. Press back and logout to finish. 10 Nov 2014