Atlantic Salmon Egg Incubation Experiment Phase III

Transcription

1 Atlantic Salmon Egg Incubation Experiment Phase III 00 Ontario Streams Technical Report No Christine Pritchard Project Biologist, Ontario Streams December 0 th, 00

2 Table of Contents Introduction.... Site Locations and Selection. Methods Direct deposit of eyed-eggs into gravel.. 8 Scotty boxes with screens, above gravel incubation Incubation tubes Direct deposit of swim-up fry into current. 0 Results.. Discussion 4 Conclusion.. Appendices Appendix A: Winter/Spring 00 Incubation Experiment Observations of Tested Methods at Upper Humber Watershed Sites Appendix B: May 009 Incubation Tube Atlantic Salmon Fry Survival Results... Appendix C: Wetted Width of 00 Electrofishing Sites Showing Positive Atlantic Salmon Results... Appendix D: Field Photographs. Appendix E: Field Collection Records for August 00 Fingerling Survival Monitoring. 6 ii

3 List of Figures Figure a-h: Location of 00 Eyed-Egg and Swim-up Fry Incubation Experiment.. -6 List of Tables Table : UTM Coordinates of Atlantic Salmon Eyed-Egg and Swim-up Fry Incubation Sites Table : Stocking Data for 00 Atlantic Salmon Eyed-Egg and Swim-up Fry Incubation Sites Table : Survival Rates of Atlantic Salmon Eyed-Eggs to Fry Stage Using Screened Scotty Boxes. Table 4: Survival Rates of Atlantic Salmon Eyed-Eggs to Fry Stage Using Incubation Tubes. Table : Fish Species Captured During August 00 Electrofishing Surveys.... iii

4 Introduction Atlantic salmon have been extirpated from Lake Ontario waters for over 00 years and likely from the Humber River headwaters for over 0 years. With the help of current restoration efforts mitigating barriers and reopening access to headwater tributaries for river spawning species like salmon, reintroduction to watersheds like the Humber River is possible. With efforts already well underway in the Credit River, Duffins Creek and Cobourg Creek, many lessons have been learned when it comes to raising and stocking hatchery raised fish. Unfortunately with hatchery raised fish comes expense with care and feeding. There are limitations in the number of eggs a hatchery can handle, thus restricting stocking scope. In addition, there are instances where observed behavioural changes occur in the fish produced. The practice of incubating Atlantic salmon eyed-eggs directly in headwater creeks, whereby viable fingerlings are produced, could be a tool in future reintroduction efforts, both in the Humber and other watersheds if a successful method can be proven. In 008 and 009, eyed-egg incubation methods were tested within Coffey Creek, Hopeful Creek and the headwaters of the main Upper Humber River. In 00, further experimentation with Direct Deposit into Gravel; and Scotty Boxes with Screens, Above Gravel Incubation; was attempted in the Upper Humber watershed to compare success rates up and downstream of road crossings. A new method, Incubation Tubes, was developed and tested at one location in Coffey Creek. The stocking success of Atlantic salmon swim-up fry was also tested between select sites in the Main Upper Humber and Cold Creek. Site Locations and Selection Coffey Creek, a headwater tributary of the Upper Humber, was chosen as the main location of this egg incubation experiment. The headwaters of the main branch of the Upper Humber, as well as Hopeful Creek and Cold Creek were additionally selected as incubation locations. These creeks display the relatively clean, coldwater habitat necessary for Atlantic salmon spawning. High gradient, fast flowing currents over cobble substrate can be found in several locations in the upper end of these watercourses. Two stocking sites further downstream on the main branch of the Upper Humber were also tested for incubation success. Until the 008 eyed-egg incubation experiment, Atlantic salmon juveniles had been absent from these tributaries for over 0 years. Figure shows the locations of the selected sites and Table summarizes the UTM coordinates for each. Sites were chosen based upon:. A fast flowing current and cobble substrate were present with minimal silt buildup. It was noted that stream reaches immediately downstream of a road crossing often had a significant amount of silt accumulation over the natural cobble and gravel substrate due to runoff from the dirt road.. There existed an established relationship with the site s landowner and there was no reasonable risk of the property ownership changing during the course of the experiment; or the sites were publically accessible in some way. This would guarantee us admittance for follow-up monitoring activities essential to the project.. The site had evidence of groundwater discharge within the reach. This removed the concern for anchor ice formation.

5 4. An attempt was made to pair several of the sites based on their proximity to a road crossing; with one site experiencing more turbidity due to its downstream location to a road, and one site experiencing less turbidity due to its upstream location. As a result, the Fimes Property, Stewart Property, Galloway Property, Coffey Creek Farm, and the Markoff Property were chosen as site locations in Coffey Creek. A private property upstream of Hwy 9 and Chicco s Restaurant were chosen as site locations in the Main Upper Humber, as well as two sites, Castlederg and Humber Station, further downstream on the main branch. Two sites were chosen in Hopeful Creek, one on the upstream side of Humber Station Road, and one downstream. An additional site on Cold Creek, upstream of Concession was selected. These locations are displayed in Figures a-h below. Figure a: Location of 00 Eyed-Egg and Swim-up Fry Incubation Experiment

6 Figure b: Both Scotty boxes and the Direct Deposit methods were employed within the Stewart Property By-Pass Channel. This location was expected to experience minimal to no turbidity due to its headwater location, upstream of any roads. Both methods were tested at the Fimes Property up and downstream sites. The upstream site was expected to experience the most turbid conditions during the spring runoff period. All Scotty boxes at these three sites were placed perpendicular to the flow. Figure c: Incubation tubes and one Direct Deposit pit were situated at the Galloway property, immediately downstream of the dam. One Scotty box was secured on the Coffey Creek Farm property in the pool downstream of Finnerty Sideroad. Scotty boxes and Direct Deposit methods were also used at the upstream and downstream sites on Coffey Creek Farm. All Scotty boxes were positioned parallel to the flow of the stream. The Finnerty branch is known to experience more turbid conditions during runoff, and this feeds into the main branch of Coffey Creek between the Coffey Creek Farm upstream and downstream sites.

7 Figure d: One Scotty box was secured in a pool at a base of a run in Coffey Creek on the Markoff Property. The Scotty box was positioned perpendicular to the current. The Direct Deposit method was also tested at this site, immediately downstream of the property s footbridge. As this site is further down in the watershed, turbid conditions during runoff events are more likely and severe compared to the upstream Coffey Creek sites. Figure e: Swim-up fry were stocked on a section of the main Upper Humber, downstream of a dam, on this private property. 4

8 Figure f: Chicco s is a restaurant off of the north side of Hwy 9. The main Upper Humber flows adjacent to this parking lot before crossing underneath the road. The Direct Deposit into Gravel method was used to stock this site. Figure g: The Castlederg and Humber Station sites are located on a wider, downstream section of the main Humber River, in comparison to Chicco s. The Direct Deposit method was employed at these two locations. Direct Deposit and Scotty Boxes were used up and downstream of Humber Station Road on Hopeful Creek. The Scotty boxes were positioned parallel to the flow. It was expected that the downstream site would experience more turbid conditions during runoff events due to its downstream position from the road.

9 Figure h: Swim-up fry were stocked on Cold Creek, upstream of Concession Road. Table : UTM Coordinates of Atlantic Salmon Eyed-Egg and Swim-up Fry Incubation Sites Site Name Chicco s Direct Deposit Site Humber Station Road Direct Deposit Site Castlederg Road Direct Deposit Site Markoff Property Direct Deposit Site Coffey Creek Farm Downstream Direct Deposit Site Coffey Creek Farm Upstream Direct Deposit Site Fimes Property Downstream Direct Deposit Site Fimes Property Upstream Direct Deposit Site Stewart Property Direct Deposit Site Hopeful Creek Downstream Direct Deposit Site Hopeful Creek Upstream Direct Deposit Site Zone 7T 7T 7T 7T 7T 7T 7T 7T 7T 7T 7T Easting Northing Markoff Property Screened Scotty Boxes Coffey Creek Farm Downstream Screened Scotty Boxes Coffey Creek Farm Upstream Screened Scotty Boxes Coffey Creek Farm Finnerty Road Screened Scotty Boxes 7T 7T 7T 7T Fimes Property Downstream Screened Scotty Boxes Fimes Property Upstream Screened Scotty Boxes Stewart Property Screened Scotty Boxes Hopeful Creek Downstream Screened Scotty Boxes Hopeful Creek Upstream Screened Scotty Boxes 7T 7T 7T 7T 7T Galloway Incubation Tubes and Direct Deposit 7T Private Property Swim-up Fry Stocking Cold Creek Swim-up Fry Sticking 7T 7T 80, to 844, , 4864 to ,

10 Methods Three Atlantic salmon eyed-egg incubation methods were tested in 00. A fourth method was used to incubate and stock Atlantic salmon swim-up fry. The incubation methods employed were: Direct deposit of eyed-eggs into gravel Screened Scotty boxes, above gravel incubation Incubation tubes, in gravel Direct deposit of swim-up fry into current The locations; conditions at the time of stocking; and numbers of eyed-eggs/swim-up fry incubated at each site are summarized in Table below. Table : Stocking Data for 00 Atlantic Salmon Eyed-Egg and Swim-up Fry Incubation Sites Direct Deposit into Gravel - Eyed Eggs Site Name Watercourse Chicco s Humber Station Road Castlederg Road Markoff Property Main Humber Main Humber Main Humber Coffey Creek Coffey Creek Farm Downstream Site Coffey Creek Farm Upstream Site Fimes Property Downstream Site Fimes Property Upstream Site Coffey Creek Stewart Property Coffey Creek Coffey Creek Coffey Creek Coffey Creek Galloway Property Hopeful Creek Downstream Site Hopeful Creek Upstream Site Coffey Creek Hopeful Creek Hopeful Creek Screened Scotty Boxes, Above Gravel Incubation Markoff Property Coffey Creek Coffey Creek Farm Downstream Site Coffey Creek Farm Upstream Site Coffey Creek Farm Finnerty Road Site Fimes Property Downstream Site Fimes Property Upstream Site Coffey Creek Stewart Property Coffey Creek Coffey Creek Coffey Creek Coffey Creek Coffey Creek Date Stocked January 7, 00 January 6, 00 January 6, 00 December, 009 December, 009 December, 009 December, 009 December, 009 December, 009 February, 00 December, 009 December, 009 December, 009 December, 009 December, 009 December, 009 December, 009 December, 009 December, Water Temp (C) C 0C 0C 0.9C Air Temp (C) -C 0C 0-4C -0.7 # of Individuals Incubated Approx. 0,000 Approx.,000 Approx., C -.0C 0.C C -7.6C 0.9C -7.6C 0 0.8C -7.6C 0.0C 0.9C -.0C -. Approx C C C -.0C 400.C C C -7.6C 400.9C -7.6C C -7.6C 400

11 Hopeful Creek Downstream Site Hopeful Creek Upstream Site Hopeful Creek Hopeful Creek Incubation Tubes, in Gravel Galloway Property Coffey Creek Direct Deposit of Swim-up Fry into Current Private Property Main Humber Cold Creek Cold Creek December, 009 December, C C February, 00.0C -.0C 900 April 8, 00 April, 00 N/A N/A N/A N/A 4,04 09 In each case, the Atlantic salmon eyed-eggs were obtained from the Harwood Fish Culture Station near Port Hope, Ontario. The eggs that would later be released as swim-up fry were raised in a Heath Incubator setup by a local club for several months. The remaining eyed-eggs used were immediately established at sites and monitored as follows: Direct deposit of eyed-eggs into gravel On the dates indicated in Table above, Ontario Streams staff walked the stretch of creek flowing through the Direct Deposit sites and located open riffles with no ice cover or evidence of anchor ice formation. At each site, when necessary, any thin ice that had formed on the surface at the edges of the stream banks was broken up to make the openings larger. At the Fimes property, Stewart Property, Galloway Property, Coffey Creek Farm and Hopeful Creek sites sediment and rock was raked to the side to create a cleared pit similar to what an adult salmon would create during natural spawning activities. Each pit was approximately 6 deep,.0m wide and.m long. A garbage bin with the bottom cut out was then placed and held firmly in the pit to eliminate any interference from rushing water. Gravel and rock was filled in around the outside edges of the bin. Medium sized rocks (4 to 8 diameter) were added to the area inside the bin to create a rough surface in the pit that would help to catch the eggs. Eyed-eggs were then slowly poured from their containers into the calm water within the garbage bin. The eggs were allowed to settle amongst the substrate and then to 6 cobble was placed by hand into the bin to cover the eggs under a shallow layer. Once the eggs and cobble had settled, the garbage bin was slowly pulled up and removed. The bin was then placed downstream of the deposited area, either within the same pit, or in a new pit, and the process was repeated. The eggs were spread out so that approximately 00 to 00 eggs were deposited in each circle created by the garbage bin. At the Humber Station, Castlederg and Chicco s stocking sites a slightly different method was employed. Pits were created as described above. Then 7.mm cobble purchased from James Dick Aggregates was poured into the pit to create a rough bottom layer approximately - thick. The eggs were added using the garbage bin as described above. At the Chicco s site in-stream cobble was used to cover the eggs. At the Humber Station and Castlederg sites the purchased cobble was added to create a - layer over the eggs. Ontario Streams staff returned to each site approximately once every two weeks until April 0th, 00. During each visit visual observations were made concerning silt accumulation; rock movement; ice and snow cover. During the May site visit, attempts were made to spot any Atlantic salmon fry in the vicinity of the incubation sites. Photographs of each site were taken during each visit to document conditions. Over several days at the beginning of September 00, these sites were electrofished to determine the presence of Atlantic salmon fingerlings. A 00m survey was conducted at each site, with the 0m point 8

12 being situated in the middle of the stocking area unless site constraints (i.e. property lines, roads) prevented this. A backpack electrofisher and -4 netters were used to sample the locations. Upon completion of the electrofishing survey, the fish caught were identified according to species; their total length and weight were measured. The fish were then released back into the stream. In addition, the wetted width of the stream was measured using a measuring tape at 0 locations (approximately 0m apart) within the sampled reach. This information was then used to determine the number of Atlantic salmon fingerlings per m. Scotty boxes with screens, above gravel incubation Screened Scotty box incubators were positioned at 9 locations in Coffey Creek and Hopeful Creek in the same vicinity of the Direct Deposit pits at the Stewart, Fimes and Markoff properties; Coffey Creek Farm; and Hopeful Creek. At each location, sites were chosen based on water depth (i.e. Scotty boxes must be secured below any suspected ice formation), the presence of a stable object on which to attach the boxes (i.e. a submerged branch, root system, or cinderblock), and sufficient flow to keep the incubated eggs aerated (i.e. boxes positioned within a run or at the base of a riffle). The boxes were positioned so that they were submerged. At some locations the boxes were placed with the flat surface of the box facing perpendicular to the current. Rock and cobble was then piled around the boxes to provide protection from the force of the flow, while still allowing aeration of the eggs. The boxes at the remaining sites were placed parallel to the flow of the creek, and minimal rock was placed around the containers to hold them in place, but not block any flow. A wire was fed through the corner holes of each box and then tied to the submerged object. A gap of approximately.inches was created between each box by dropping a few small rocks in between the boxes to maintain this distance. Two hundred Atlantic salmon eggs were contained in each box. The Scotty boxes were monitored by Ontario Streams staff approximately once every two weeks until April 0 th, 00. During each visit visual observations were made concerning silt accumulation; rock movement; ice and snow cover. Photographs of each site were taken during each visit to document conditions. On May th 00, the Scotty boxes were removed from the stream and checked for egg survival. The rocks over top of each site were removed by hand and the Scotty boxes were taken from the stream and placed in a cooler full of creek water. Each Scotty box was pulled out one at a time. A photograph of the outside of the box was taken to document silt accumulation. The bolts holding the boxes closed were unscrewed and the box was opened over top of a water-filled bin. A second photograph was taken of the opened box, again to document silt accumulation. The number of live Atlantic salmon fry; and the dead, but developed fry were counted and tallied. The living fry were placed in a bucket of water while the above process was completed for each Scotty box in the grouping. After all of the boxes were removed and fry were tallied, the living fry were released into the stream by gently pouring out the fry in sheltered areas (i.e. behind woody debris or large rocks) in the reach. In September 00, these sites were electrofished to determine the presence of Atlantic salmon fingerlings. The 00m survey described in the above Direct deposit of eyed-eggs into gravel section overlapped with these sites. A 0m electrofishing survey was conducted at the Coffey Creek Farm Finnerty Rd site because the Scotty box had been placed very close to the road crossing, and we did not have access to the upstream property. Therefore, any fry dispersing downstream of the road culvert were monitored for. 9

13 Incubation tubes In early 00, 8 incubation tubes were constructed. 4 diameter ABS pipe was cut into ft sections. diameter holes were drilled through the sides of each tube (approximately - holes per tube). A cap was glued to the end of each tube and window screening was glued around the sides. A removable cap was placed on the other end of each tube. On February, 00, a cobble riffle free of ice was located immediately downstream of the Galloway Property dam. A trench, perpendicular to the current, was created using rakes and moving rock by hand. An incubation tube was filled with 7.mm cobble purchased from James Dick Aggregates in alternating layers with approximately 0 eyed eggs; approximately 0 eggs per tube. The tube was capped and then placed in the trench, perpendicular to the flow of Coffey Creek. Two tubes were placed in each trench. The trench was filled in and the tubes were covered with on-site -6 cobble. This process was repeated - feet downstream with another trench, and so on, until 8 tubes were installed at this location. The tubes were monitored by Ontario Streams staff approximately once every two weeks until April 0th, 00. During each visit visual observations were made concerning silt accumulation; rock movement; ice and snow cover. Photographs of each site were taken during each visit to document conditions. On May 8, 00 the tubes were removed from the creek. Each tube was gently dug out from the cobble and placed one at a time in a bin full of water. A photograph of the outside and inside of each tube was taken to document silt accumulation. Each tube was carefully uncapped and the contents poured out into the bin of water. Using small aquarium nets, the living and dead fry were sorted from the cobble. The number of fry per tube was tallied before releasing the fry into a sheltered area at the edge of the creek. On August 7th, 00 the site was electrofished to determine the presence of Atlantic salmon fingerlings. A 00m survey was conducted, with the end point being situated at the dam overflow. A backpack electrofisher and netters were used to sample the location. Upon completion of the electrofishing survey, the fish caught were identified according to species; their total length and weight were measured. The fish were then released back into the stream. In addition, the wetted width of the stream was measured using a measuring tape at 0 locations (approximately 0m apart) within the sampled reach. This information was then used to determine the number of Atlantic salmon fingerlings per m. Direct deposit of swim-up fry into current Eyed-eggs obtained from the Harwood Fish Culture Station were raised in a Heath Incubator by a local club. Every two to four days the eggs were inspected and dead or fungus infected eggs were picked out with tweezers and discarded. On April 8th and April rd, 00 the fry were enumerated and transferred into oxygenated bags of water. The bags were placed in coolers and transferred to the stocking sites at the Private Property upstream of Hwy 9 and Cold Creek. At the site the fry were transferred into buckets of water. Ontario Streams staff walked along the creeks in an upstream direction, pouring out fry in sheltered areas in the riffle and run sections downstream of the property s dam. Approximately 4,04 fry were deposited at the Private Property site and 09 fry at the Cold Creek site. 0

14 On September 4th, 00 the Private Property site was electrofished to determine the presence of Atlantic salmon fingerlings. A 00m survey was conducted, with the end point being situated in the dam overflow. A backpack electrofisher and netter were used to sample the location. On August st, 00 a 00m electrofishing survey was conducted at the Cold Creek site. Upon completion of the electrofishing surveys, the fish caught were identified according to species; their total length and weight were measured. The fish were then released back into the stream. In addition, the wetted width of the stream was measured using a measuring tape at 0 locations (approximately 0m apart) at the Private Property sampled reach and 0 locations at the Cold Creek site sampled reach. This information was then used to determine the number of Atlantic salmon fingerlings per m. Results Turbid water conditions were not observed during the winter/spring monitoring period at the Stewart property; Galloway property; Coffey Creek Farm upstream site; and Hopeful Creek, both up and downstream sites (See Appendix A). Turbid conditions were noted on April 6 th at Chicco s; Coffey Creek Farm Finnerty site and Coffey Creek Farm downstream site; and both upstream and downstream sites on the Fimes property. On March 9th and April 6 th highly turbid conditions were noted at the Castlederg and Humber Station sites. Also during the winter/spring monitoring period, sediment buildup was noted around many of the Scotty boxes. Significant deposition around the boxes occurred at both the upstream and downstream sites on the Fimes Property; Stewart Property; and Markoff Property. All of these Scotty boxes were placed perpendicular to the flow of the current. Also, deposition took place at the Hopeful Creek upstream site. These Scotty boxes were placed parallel to the flow. No sediment buildup was noted at the Coffey Creek Farm upstream and downstream sites. These boxes were placed parallel to the current. Minor sediment accumulation was noted at the Hopeful Creek downstream site before the boxes shifted. These boxes had been placed parallel to the flow. Several Scotty boxes shifted out of position during the course of the experiment. Minor shifting occurred with the Scotty boxes at the Coffey Creek Farm upstream and Finnerty Road sites. The Finnerty Road boxes remained parallel to the current and therefore were not repositioned. The upstream site boxes were repositioned on March 9th and remained in place until they were removed in May. The Scotty boxes in Hopeful Creek experienced a larger disturbance. The upstream boxes were repositioned on April 6th to decrease the potential for sediment accumulation. They were next checked on April 0th and had been washed out of position. A portion of one box was out of the water. On March 9th it was noted that the Hopeful Creek downstream Scotty boxes had shifted and they were repositioned parallel to the flow. On April 0th it was noted that the boxes had shifted once again, however in this instance they remained parallel to the stream s flow and so they were left as is. The Scotty boxes were removed on May th, 00. Almost all of the screens had come loose, to some extent, at each site with the exception of the Markoff property. The survival data for each site is summarized in Table. The Fimes upstream site Scotty boxes contained the most living fry (0.8%), followed by Hopeful Creek downstream site (6.%); Coffey Creek Farm upstream site (6.%); and Markoff Property (6.0%).

15 The Galloway Incubation Tubes were opened on May 8th, 00. As summarized in Table 4, a total of (4.%) living fry were released from these tubes. An additional 404 developed but dead fry were also tallied. Overall, 6 eggs developed into fry at this site, or approximately 69.4%. Phase III of the Atlantic salmon eyed-egg in-stream incubation experiment produced viable Atlantic salmon fingerlings within 4 of the reaches sampled with the backpack electrofisher in August/September 00. Coffey Creek Farm downstream site; Fimes property upstream site; Hopeful Creek downstream site; and Private Property upstream of Hwy 9 all produced evidence of fingerling survival. Table summarizes the results. The Private Property site where swim-up fry were stocked produced the highest density of 0.04fingerlings/m. One yearling was caught during the 00 August/September electrofishing surveys. This individual was captured on the Galloway property on August 7, 00, weighing g and measuring 47mm in length. Table : Survival Rates of Atlantic Salmon Eyed-Eggs to Fry Stage using Screened Scotty Boxes Site Parallel or Alive % Developed, Screen Perpendicular to Survival but dead Loose? flow Hopeful Creek, Scotty Parallel 0 6.% Yes downstream site Box # Scotty Parallel 6 A little Box # Hopeful Creek, Scotty Parallel 0.% Yes upstream site Box # Scotty Parallel 6 0 Yes Box # Markoff Property Perpendicular 6.0% 0 No Coffey Creek Farm, Scotty Parallel 0.% 8 Yes downstream site Box # Scotty Parallel 6 Yes Box # Coffey Creek Farm, Scotty Parallel 6.% Yes upstream site Box # Scotty Parallel 0 No Box # Coffey Creek Farm, downstream Parallel 0.% Yes of Finnerty Sdrd Stewart Property By- Scotty Perpendicular 0.8% 0 Yes Pass Channel Box # Scotty Perpendicular 0 0 Yes Box # Fimes, Upstream Site Scotty Perpendicular 0.8% No Box # Scotty Perpendicular 8 8 Yes Box #

16 Fimes, Downstream Site Scotty Box # Scotty Box # Perpendicular Perpendicular.8% Yes Yes Table 4: Survival Rates of Atlantic Salmon Eyed-Eggs to Fry Stage using Incubation Tubes # Eyed Eggs Living Fry Dead, But Total Fry % Eggs Developed Stocked Developed Fry to Fry Stage Approx % Species that were captured at the incubation sites using a backpack electrofisher in August 00 included: Table : Fish Species Captured During August 00 Electrofishing Surveys Stocking Site Method(s) Used Atlantic Salmon Stocked at Site 0,000 Atlantic Salmon Captured Species Sampled Chicco s Direct Deposit into Gravel 0 Direct Deposit into Gravel,000 0 Castlederg Road Direct Deposit into Gravel,000 0 Markoff Property Screened Scotty Boxes and Direct Deposit into Gravel Direct Deposit into Gravel and Screened Scotty Boxes Direct Deposit into Gravel and Screened Scotty Boxes Screened Scotty Boxes,000 0,00, Direct Deposit into Gravel and Screened Scotty Boxes Direct Deposit into Gravel and Screened Scotty Boxes Direct Deposit into Gravel and Screened Scotty Boxes Direct Deposit into Gravel and Screened Scotty Boxes,00 0 Brown trout; brook trout; American brook lamprey; mottled sculpin; white sucker; creek chub; balcknose dace; central mudminnow; Johnny darter Brown trout; creek chub; blacknose dace; common shiner; white sucker; Northern hognose sucker; longnose dace; Johnny darter Brown trout; longnose dace; white sucker; blacknose dace; common shiner; creek chub; Johnny darter Brown trout; brook trout; creek chub; white sucker; mottled sculpin; Northern redbelly dace; blacknose dace; longnose dace Brown trout; brook trout; blacknose dace; creek chub; mottled sculpin, longnose dace, white sucker Brown trout; brook trout; mottled sculpin; longnose dace; creek chub; blacknose dace; white sucker Brown trout; brook trout; Northern redbelly dace; creek chub; mottled sculpin; longnose dace; blacknose dace Brook trout Humber Station Road,00 Brook trout,00 0 Brook trout 906 Brown trout; brook trout; blacknose dace; creek chub Coffey Creek Farm Downstream Site Coffey Creek Farm Upstream Site Coffey Creek Farm Finnerty Sideroad Site Fimes Property Downstream Site Fimes Property Upstream Site Stewart Property Hopeful Creek Downstream Site

17 Hopeful Creek Upstream Site Galloway Property Private Property, Upstream of Hwy 9 Cold Creek Direct Deposit into Gravel and Screened Scotty Boxes Incubation Tubes and Direct Deposit into Gravel Direct Deposit of Swim-up Fry into Current Direct Deposit of Swim-up Fry into Current Brown trout; creek chub; blacknose dace,000 (yearling) 4,04 Brown trout; brook trout; mottled sculpin; white sucker; blacknose dace; longnose dace; creek chub; golden shiner; largemouth bass Brook trout; white sucker; mottled sculpin; creek chub; blacknose dace 09 0 Brown trout; rainbow trout; white sucker; mottled sculpin; Discussion It is important to note that because the screens of most of the Scotty boxes became loose over the course of this experiment, it cannot be assumed that the number of fry released from the boxes in May represent the total number of living fry as individuals could have escaped on their own over the spring. In addition, as there are no barriers blocking the upstream and downstream ends of each reach, it cannot be assumed that the Atlantic salmon caught during the August/September electrofishing represent the total Atlantic salmon that survived at each site. Some individuals may have migrated up or downstream out of the 00m sampled reach. Others may have successfully developed to the fry or fingerling stage, but were then eaten by predators. Therefore, the following comments are approximations based on the available data and observations. While visual observations indicated that the placement of Scotty boxes parallel to the current, instead of perpendicular, resulted in less sediment accumulation around the boxes, this did not correlate into an increase in survival to the fry stage. Of the Scotty boxes placed parallel to the current,.7% of eyedeggs were released as living fry in May, compared to 4.6% with the perpendicularly placed boxes. This slight difference between methods does not clearly indicate an advantage to either. Similarly, it was believed that Scotty boxes placed in streams experiencing less turbidity during the spring runoff period would have a better rate of survival. This theory was not supported by the 00 data. Scotty box survival was greatest at the Fimes upstream site (0.8%) which was considered to be a slightly more turbid site. Clear water sites at the Hopeful Creek downstream site (6.%) and Coffey Creek Farm upstream site (6.%) showed second and third place survival. While the Markoff property, which experiences high levels of turbidity and whose Scotty boxes became completely buried in deposited sand, showed the fourth highest rate of survival (6.0%) of all the Scotty boxes. These inconclusive patterns are supported by the August/September electrofishing results. The Hopeful Creek downstream site (low turbidity, parallel Scotty boxes) produced fingerlings, or 0.% of stocked eggs. The Coffey Creek Farm downstream site (turbid, parallel Scotty boxes) and the Fimes upstream sites (turbid, perpendicular Scotty boxes) each produced fingerling, or 0.08% of stocked eggs. There is no evidence to suggest that the incorporation of purchased 7.mm cobble into the direct deposit sites at Chicco s, Castlederg and Humber Station resulted in an increase in egg survival. No fingerlings were found at any of these sites during the August/September electrofishing. 4

18 The Incubation Tubes installed at the Galloway property showed promise. A 4.% survival of eyed-eggs to the fry stage significantly surpasses any of the Scotty box survival results over the past three years; or results of the Vibert boxes tested in 008. If the additional 404 dead but developed fry had in fact survived until the May 8th release date, this success rate would have increased to 69.4%! It is suspected that miscommunication with the landowner may have resulted in the death of some of these fully developed fry. Immediately upstream of the incubation site is the Galloway property online pond. Each fall the landowner removes some of the stop logs in the dam, and he replaces them after the spring melt to avoid flooding on his property. When the stop logs were replaced in the spring, a couple weeks before the tubes were removed, there would have been periods of significantly reduced flow directly downstream of the dam. These conditions may have negatively affected the fry. While depth measurements were not taken, many of the Incubation Tubes that produced better results seemed to be placed in slightly deeper trenches. They may have remained more saturated with water during these times of low flow. Overall, it is believed that the Incubation Tubes should be tested further in 0 at multiple sites. The stocking of swim-up fry also showed potential as a future technique in the Upper Humber watershed. This method produced 0.04fingerlings/m which was the highest density observed at all of the 00 sites. It should also be noted that 4,04 fry were stocked here, an amount much greater than at any of the Scotty box/direct Deposit sites. Therefore, the high density of fry directly resulted in the high density of fingerlings. Percentage-wise, 0.087% of the swim-up fry were caught as fingerlings. This is comparable to the Coffey Creek Farm downstream site and Fimes property upstream site (0.08%), and less than the Hopeful Creek downstream site (0.%). However, the time it took to stock the 4,04 fry was significantly less than what it would take to stock the same amount of eyed-eggs in Scotty boxes or through the Direct Deposit method. This time factor makes the swim-up fry technique very attractive when dealing with a large-scale stocking program. In contrast, the swim-up fry stocked at the Cold Creek site did not produce any Atlantic salmon fingerlings in the August electrofishing survey. This does not conclusively indicate that no Atlantic salmon survived to this stage at this site, but it is in stark contrast to the other swim-up fry stocking site. Less ideal habitat conditions, as well as predation by rainbow trout and brown trout, were likely factors here. Other locations within Cold Creek should be surveyed and pursued as 0 stocking locations. The swim-up fry would require less resources and time to raise when compared to hatchery raised fry or fingerlings. Smaller incubation trays could be set up and run by volunteer groups. In-class hatcheries have been implemented at many schools in the Credit River watershed. In the Upper Humber area, several elementary and high schools could be approached for the same purpose. The Islington Sportsmen s Club also runs a small hatchery and they should be approached to participate in this program as well. Conclusion Results of Phase III of the Atlantic salmon incubation experiment indicated that the Direct Deposit into Gravel method or the implementation of screened Scotty boxes still cannot be relied heavily on as methods for Atlantic salmon reintroduction. Experimentation with parallel versus perpendicular placement of Scotty boxes; and turbid versus less turbid stocking sites did not result in any noticeable trends that would influence the implementation of these techniques. While there has been some

19 success with Scotty boxes at certain sites, their implementation on a large scale would be extremely time consuming. The Incubation Tubes showed a great deal of potential as an eyed-egg stocking technique and it is recommended that further experimentation with this method be continued in 0 at multiple sites. It is also recommended that the majority of the 0 eggs be raised to the swim-up fry stage, with the help of volunteers, and stocked into appropriate creeks in April 0. The time and resources invested in raising the eggs to this stage will be compensated for in the time saved during the winter/spring biweekly monitoring and stocking, and the improvement in survival and density results. 6

20 Appendix A: Winter/Spring 00 Incubation Experiment Observations of Tested Methods at Upper Humber Watershed Sites UTM January 8, 00 January, 00 February 4 th, 00 (Air: -C) February 8 th, 00 (Air: C) March th, 00 (Air: 4C) March 9 th, 00 (Air: 8C) Fimes Upstream Site Direct Deposit 7T 0874, Air: -9C; Water:.C -surface ice forming u/s of rocks -normal flow -clear water Water C -channel open, clear; normal flow -site blends with surroundings; no excess sediment buildup noted Water C -clear, normal flow -no excessive sediment buildup -channel free of ice and snow Water C -water clear; normal flow -no ice; ground is snow covered -no excessive sed. Buildup noted Water C -no ice/snow in channel; some snow left on bank -water level up, but clear -no sediment build up over site Fimes Upstream Site Scotty Boxes 7T , Air: -9C; Water:.C -surface ice forming u/s of rocks -normal flow -clear water -open, clear; normal flow -silt depositing immediately downstream of SBs -clear, normal flow -channel free of ice and snow Lots of silt depositing d/s side of boxes in side pool -footprints in silt d/s? -clear, normal flow -no ice, snow -moved rock to check sed. Buildup- bottom half of SB packed in silt; should have top of run? Fimes Downstream Site Direct Deposit 7T 0876, 4869 Air: -8.C; Water:.6C -channel clear of ice and snow in middle, thin ice at sides approx. cm thick -no obvious sediment buildup -normal flow through riffle, water clear -turkey tracks Air C, Water C -clear of ice and snow -clear, normal flow -can t tell difference btw red and surroundings; natural level of sed. deposit Air C, Water C -very silty bottom d/s of site -rocks in place -no ice or snow -normal, clear flow -no sed buildup apparent based on top of SB, just under surface rocks Air C, Water C -some sand depositing btw rocks, but reasonable -clear flow; no ice/snow over site Water C -thin ice and snow covering site approx. inch thick -open riffle at bottom end -water clear; normal flow -channel 7% open over site - ice and snow thick -no excessive sediment buildup -water clear; normal flow -site clear of ice and snow -normal, clear flow -no sediment buildup over site, however indistinguishable from surrounding area 7 April 6th, 00 (Air: C; light rain in AM) -water slightly cloudy and up from AM rain; can see bottom -no sediment buildup April 0th, 00 (Air: 9C) Water 6C -water clear; norm flow -cannot distinguish pits -no excessive sediment buildup -sediment build up d/s of site and within rock pile -water clear; flow higher than normal Water 6C -water slightly cloudy -sediment build up under rock pile; moved rock to increase flow, but too late to affect survival -lots of sediment buildup under rocks around SBs -water clear, normal flow -rock unmoved Water C -water clear; flow might be up slightly -no ice/snow; small amt of s now left on ground Water 7C -Water slightly turbid, but can see bottom; flow up a bit -no sediment buildup -clear, norm flow -no excessive sediment buildup -pit not distinguishable from surroundings

21 Fimes Downstream Site Scotty Boxes 7T 09, 4866 Stewart Property Direct Deposit 7T , Stewart Property Scotty Boxes 7T 0870, 4868 Coffey Creek Farm Finnerty Road Scotty Boxes 7T 08896, 486 across ice Air: -8.C; Water:.6C -channel covered in ice and snow, but open of ice in middle above SB - water clear, normal flow Air:-8.7C; Water:.C -ice and snow over most of channel, but open spot in site a couple inches wide -ice approx. in thick; only on surface -flowing good, clear below Air:-8.7C; Water:.C -covered in ice and snow; ice not solid -ice approx. in thick -open water under snow in d/s riffle -good, clear flow below Air: -7.C; Water: 0.8C -pool covered by thin ice and snow -open at culvert mouth -d/s water clear -hear good flow out of culvert Air C, Water C -ice and snow at edges -flowing clear over SB rocks -no sediment accumulation apparent Air C, Water C -patchy ice at surface around rocks -normal, clear flow beneath -sand visible btw rocks, but no large accumulation seen -thin ice covering site, approx cm thick; small gaps in ice -hear water flowing beneath Water: C -ice and snow thick above site; small open holes -water clear; normal flow -can t see SBs Water C -channel mostly clear of ice and snow -ice ledge over rock pile around SBs -water clear; normal flow -no ice/snow -water clear; up slightly -rocks unmoved -sediment packed around SB under rocks -water slightly turbid, but can see bottom; flow up a bit -sand and organic matter packed around SBs under rocks -Water C -some organic sediment around SB, but not buried -water clear, flow norm Water C -site covered in thin ice, approx inches above water level; ice broke when stomped on -water clear; normal flow Water C -open patch over ½ of site -ice + snow = thick -water clear; normal flow -sand deposited in between rocks Water 0C -channel clear of ice and snow at site -sand buildup between river rock bottom Water C -channel free of ice and snow -water clear; flow up slightly -sand deposited between rocks, but nothing excessive over site Water 7C -water clear -flow up slightly due to rain -sand in btw rocks; no excessive buildup Water 6C -sand building up between rocks -clear, normal flow See above -covered in ice and snow; ice did not break when stomped on -can hear flowing water beneath -site covered in ice and snow can t stomp through -hear good flow beneath ice -channel open at site -rocks moved, can see top edge of SBs -did not feel sediment buildup around boxes; moved rocks back -screen on box coming loose -channel free of ice and snow -water clear; flow up slightly -no sediment buildup around SBs -water clear; flow up slightly -sand and organic debris packing around boxes -clear, norm flow -rock unmoved -sediment buildup around SBs; screens loose Air C, Water 0C -ice on top of pool; open at culvert mouth -water clear, normal flow -can t see SB due to ice cover Water Temp N/A -pool and culvert spillway frozen Water N/A -pool surface frozen over -small animal tracks on ice -hear food flow coming through culvert underneath ice Water 0C -small opening in centre of pool; ice and snow covering rest of channel Water C -water clear; up slightly -no ice/snow -no sediment buildup -SB on its side, but holes still parallel to current; did not reposition, stable Water 7C -water turbid -can barely see SB, but doesn t seem to have sediment build up around it Water 6C -SB unmoved; parallel to current; slightly on side -some sediment on screen, but no buildup around box -water clear, 8

22 as is Coffey Creek Farm Upstream Site Direct Deposit 7T 08904, 4868 Air: -7.; Water:.4C -channel open, clear, no obvious sediment buildup -good flow Air C, Water C -clear of ice and snow -water clear; normal flow -no sed. buildup Water C -open, clear; normal flow -ice at sides of channel -no significant sediment buildup -open channel -clear water; normal flow -no sediment buildup apparent Water C -channel free of ice and snow -normal, clear flow -no sediment buildup Coffey Creek Farm Upstream Site Scotty Boxes 7T 08904, 4868 Air: -7.; Water:.4C -thin ice and snow over most of channel -appears open under where SB should be thin patchy ice at surface only Air C, Water C -water clear, normal flow -ice at sides; ice above water level over SB, can t see SB due to ice shelf -thin ice, approx cm, covering site -clear, normal flow beneath Water C -ice and snow at sides of channel -water clear; normal flow -can barely see SBs under ice ledge looks like they are still in place -clear, normal flow -channel free of ice and snow; still ice shelf overhanging bank -SB appear unmoved; no sediment buildup felt around boxes Coffey Creek Farm Downstream Site Direct Deposit 7T , 4864 No Temp Data Thermometer broke -open; strong, clear flow, no sediment buildup Water 0C -strong, clear, normal flow -no sediment buildup apparent -channel open; ice and snow only at edges -strong, clear, normal flow -no sediment buildup apparent Water 0C -clear, normal flow -no sediment build up - no ice/snow in channel Coffey Creek Farm Downstream Site Scotty Boxes 7T 089, No Temp Data Thermometer broke -open and flowing clear -SB visible from bank Markoff Property Direct Deposit 7T 09947, No Temp Data Thermometer broke -covered with think ice and Air C, Water 0C -strong, normal, clear flow -no sediment buildup -channel free of ice Air C, Water 0C -clear, normal flow -no sediment buildup around SBs -channel free of ice and snow Air C, Water 0C -ice and snow at edges only -channel clear, -covered with inch thick ice at surface (had to break up ice to find boxes) -clear, normal flow beneath -no apparent sediment buildup Water 0C -thin ice over site; open d/s -clear, normal flow Water C -water clear; normal flow -SBs unmoved -appear clear of sediment -thin ice u/s sides; open over boxes Water C -ice and snow over site; open d/s -water clear; 9 Water C -water clear; approx. regular flow -no observed sediment buil dup over site -no ice/snow left in channel -water clear; approx reg. flow -no ice/snow left in channel -SB shifted; now perpendicular to flow! Tried to reposition; is now upright, on its side -no sediment buildup Water C -strong flow -clear water -mink (?) d/s -no sed buildup noted -no ice/snow left Water 9C -water clear, flow close to norm. -no excessive sediment buildup -no sediment buildup around SB!! -clear, normal flow -no ice/snow in channel Water 0C -water slightly turbid, but can see bottom -no noticeable sediment buildup norm flow *camera battery died Water 8C -water clear; norm flow -no noticeable sed buildup -water clear, flow close to norm. -very little sediment accumulation on boxes seems good -flow normal, clear -SB parallel to flow; one on side, one upright -sediment on screens, but not built up around -strong flow; slightly turbid but can make out bottom Water 7C -norm, strong, clear flow -no noticeable sed buildup -no ice/snow left -clear water; flow up slightly -SBs unmoved; no sediment buildup Water 8C -water slightly turbid but can make out bottom - no sediment buildup -boxes unmoved Water C -water turbid; flow up slightly -can vaguely see stream bottom Water 8C -water very turbid; can t see bottom -clear, norm flow -SB unmoved; parallel to current -sed on screens, but not built up around Water 9C -water clear, flow norm -sand in btw rocks, but no

23 Markoff Property Scotty Boxes 7T 0990, Hopeful Creek Upstream Site Direct Deposit 7T 0970, Hopeful Creek Upstream Site Scotty Boxes 7T 0970, Hopeful Creek Downstream Site Direct Deposit 7T 0974, snow, but small opening in centre of channel -hear good flow -clear water d/s No Temp Data Thermometer broke -channel covered in ice and snow; thicker than d/s site No Temp Data Thermometer broke -mostly open over site; ice and snow all around normal flow -no sed. buildup normal flow over site -channel clear of ice and snow -approx. normal flow excessive buildup compared to surrounding area Same as above, but ice covers surface over SB, so can t see SB -water dark, but not turbid -ice over pool; open u/s -clear, normal flow -ice and snow over site + u/s and d/s -animal tracks on ice -water slightly turbid -rock and SB unmoved -SB may be buried in sand! -water turbid; flow up slightly; can vaguely see bottom -SB buried in deposited sand -water very turbid; can t see bottom -buried in sand Air C, Water 0C -clear, normal flow -no sed. Buildup -snow and ice at sides, site clear Water 0C -site covered by ice and snow at surface -hear water flowing beneath Water C -ice and snow at sides of channel -no excessive sediment buildup; sand between rocks -water clear; normal flow -clear, normal flow -channel clear of ice/snow -no observed sed. Buildup Water clear; flow up -no sed, buildup obvious over site Water 6C -water clear; flow up slightly -no notable sediment buildup No Temp Data Thermometer broke -thin ice and snow cover above; some open patches u/s and d/s -water clear; normal flow Air C, Water 0C -thin ice over most of site; channel flowing down middle, approx ft wide -water dark, but not turbid, can t see SBs -site covered by ice and snow at surface -hear water flowing beneath -channel open immediately upstream -ice + snow = thick -water clear; normal flow Water 0C -most of channel free of ice and snow -no sed. Buildup around SB, but may have shifted; not 00% parallel to flow now; feels secure -normal, clear flow Water C -SB shifted out of parallel position; fixed -water clear, flow up -silty bottom around SBs; hard to comment on sed buildup due to SB movement -no ice/snow left -water clear; flow up slightly -sediment was depositing around SB, so moved out into stronger flow No Temp Data Thermometer broke -open in centre; patchy, thin ice (/ in) and snow surrounding Air: C, Water 0C -thin ice over most of site; channel flowing down middle -water clear in open areas; Water 0C -ice and snow over site except small ft long opening -small open patch in ice ( ) -ice thick; above water level -water clear; normal flow -channel over site covered in ice/snow -u/s water clear, normal flow -clear flow; up -no observed sediment buildup -no ice/snow left -water clear; flow up slightly - no notable sediment buildup -water clear, norm flow -can t distinguish pits -no excessive sediment buildup -stream bottom gravel 40, sand 0, cobble 0 Water 6C -SB washed out of position! Part of box above water! -repositioned parallel to flow, but not expecting good results -water clear; flow normal Water 6C -water clear, norm flow -can t distinguish pits -no excessive sed buildup; stream 0

24 -clear water, normal flow Hopeful Creek Downstream Site Scotty Boxes 7T 097, Humber Station Rd 7T 0970, 4894 Castlederg Rd 7T , No Temp Data Thermometer broke -channel mostly open; small patches of ice and snow; covering SB location -good, clear flow normal flow -no clear view of bottom to see sediment buildup, don t suspect large amt Air: C, Water 0C -water still open here; ice and snow at edges -water clear; normal flow -some ice forming on top of rocks over boxes just at surface -no obvious sed. Buildup bottom gravel 0, sand 0, cobble 0 -ice over site thin; can see moving water below Water 0C -thin ice over site (. ) but channel open in patches up and d/s -may be some silt accumulation around boxes in pool -water clear, normal flow Water 0C -normal, clear flow -SB unmoved, but sed. Buildup along side, /way up box; other side seems clear Water C -SB shifted out of parallel position; fixed -clear flow; up -no ice/snow left Water 6C -water clear; flow up slightly -SB unmoved; no sediment buildup Water 0C -water clear; normal flow -channel open above stocking site; ice and snow surrounds -usual amount of sediment on bottom (gravel and sand mix) Water 0C -ice frozen over site -openings up and d/s of site -open at u/s pit I m not going out on that ice for a closer look! Water 0C -thin ( ) ice and snow over surface; rabbit tracks on ice -water clear; normal flow Water 0C -some ice cover -clear, normal flow -no significant sed. Buildup; bottom is naturally 0-40% sand Water 4C -water very turbid; can t see bottom -flow up -no ice/snow left Water 0C -water slightly turbid, but can see bottom -no excessive sediment buildup -can t distinguish pits from rest; only smaller gravel ( ) remains Water 0C -thin ice and snow over site -hear flow beneath ice -some open patches up and downstream -small animal -Water 0C -thin ice over parts of channel/site -clear, normal flow -can t comment on sediment buildup due to ice; same with u/s location Water 4C -water turbid, can barely see bottom; can t tell where pits were -no snow/ice left -same with u/s site Water 0C -water clear; flow slightly up -pits indistinguishable from surroundings -no excessive sediment buildup over sites -SB moved; lying on sides, but holes are parallel to flow and no sediment building up around; tried to secure better, not very successful -some sed build up on screen -screens coming loose Raccoon tracks in mud on bank Water 8C -cannot distinguish pits from surroundings -gravel, sand substrate Water 9C -cannot distinguish pits from surroundings -gravel, sand substrate

25 Chicco s Galloway Property Incubation Tubes and Direct Deposit 7T , Water 0C -channel free of ice over site -normal, clear flow -can see slight difference in colour of pits vs. surrounding stream bottom tracks on ice -u/s location has open patch over site; did not go out on ice to look closer for safety reasons! Water 0C -can still make out slight colour difference of pits -channel clear of ice and snow -clear water; normal flow Water C -can see blue cap edges occasionally -no sediment buildup -good, clear, normal flow; no ice/snow -can t distinguish DD pit Water 0C -pits still slightly distinguishable from surrounding substrate by lighter colour -no ice or snow in channel -water clear, can see bottom -normal flow Water C -clear, normal flow -can see edges of blue caps -cannot distinguish disturbed areas -no sediment buildup Water C -water turbid, can vaguely see bottom -water level up slightly -warm weather all week; snow melt -cannot distinguish pits from surrounding stream bottom -no ice snow left in channel Water C -4 blue caps seen, but unmoved -no sediment deposit noted Water 8C -water clear -pits blend with surrounding substrate -no sediment buildup Water 6C -norm flow; water clear -cannot distinguish pits from surroundings -no excessive sediment buildup -Water 0C -water clear; flowing strong -4 blue caps visible - no sediment buildup; moved rock to check Water 8C -flow clear, norm - blue caps visible -still some snow on S bank of online pond

26 Appendix B: May 00 Incubation Tube Atlantic Salmon Fry Survival Results Tube # TOTAL Alive Fry Dead, Developed Fry Total Fry Comments Deeper Deeper Deeper Deeper Deeper Deeper Appendix C: Wetted Width of 00 Electrofished Sites Showing Positive Atlantic Salmon Results Site Transect # Transect Wetted Width (m) Coffey Creek Farm downstream site Direct Deposit and Screened Scotty box methods Fimes upstream site Direct Deposit and Screened Scotty box methods Average Wetted Width of 00m Reach (m).8 Estimated Area of 00m Electrofished Reach. m 8 m # Atlantic Salmon Fingerlings/m

27 Hopeful Creek downstream site Direct Deposit and Screened Scotty box methods Private Property upstream of Hwy 9: Swim-up fry method m m 0.04

28 Appendix D: Field Photographs Direct Deposit into Gravel Method Shovels, rakes and boots were used to create a clean oblong pit in a cobble riffle. A bottomless garbage bin was used to create an area of still water in which the eggs or sac fry could be deposited. The garbage bin was screwed into the substrate and held firmly in place.

29 Medium sized rock was then added to the pit to create a coarse surface that would catch the deposited eggs or sac fry. Approximately 00 eggs or sac fry Atlantic salmon eyed-eggs. Rock and gravel was then gently added to the bin to cover the eggs. 6

30 The bin was then removed and the process was repeated downstream. The pits were approximately m wide by.m long 7.mm cobble purchased from James Dick was used at the Chicco s, Castlederg and Humber Station Direct Deposit sites. 7

31 Screened Scotty Boxes, Above Gravel Method A tray of Atlantic salmon eyed-eggs. 00 eggs are gently brushed into a sorting tray. One side of a screened Scotty box is placed on top and then inverted, thus dumping the eggs into the individual compartments in the Scotty box. 8

32 The second half of the Scotty box is placed and the two sides are bolted together. A finished screened Scotty box containing 00 Atlantic salmon eggs. Scotty boxes are secured underwater to an object such as a submerged branch, root or cinderblock. 9

33 Scotty boxes were placed either parallel, as seen here, or perpendicular to the current. In-stream rock was then used to provide stability to the boxes. Scotty boxes were removed from the stream in May and opened. This sediment accumulation on the outside of a 00 Scotty box was typical for all sites. Typical sediment accumulation within the 00 Scotty boxes. 0

34 The Scotty boxes had been screened to prevent the hatched fry from escaping, and therefore allow the success of the boxes at each site to be directly compared. Unfortunately the screens came loose from many of the boxes during the incubation season. Several living Atlantic salmon fry were released from the Scotty boxes in May. Incubation Tube Method The incubation tubes were made out of PVC tubing, cut into ft lengths. A cap was glued to the bottom of each tube and holes were drilled in the sides. Window screening was glued around the tube. A removable plastic cap was placed on the top of each tube.

35 The tubes were filled with 7.mm purchased cobble. with eggs layered in amongst the rock. The eggs were gently counted into the tubes using a wet feather. Approximately 0 eggs were incubated in each tube.

36 Rock at the Galloway stocking site was approximately 6 in diameter. A trench, perpendicular to the flow of the creek was dug by hand. Incubation tubes were placed in the trench, below the level of the substrate. The trenches were filled back in with rock. Flagging tape tied to the tubes, along with blue caps, were used to help identify the tube locations and facilitate in finding them in May.

37 The tubes were removed in May. They had some sediment accumulation on the outside of the screens. The tubes were opened one at a time over a water filled bin. Minimal sediment accumulation was noted inside the tubes. The rock and fry were emptied into the water filled bin and then sorted. 4

38 living Atlantic salmon fry were released from the 8 incubation tubes. 404 dead, but fully developed, fry were also found in the incubation tubes.

39 Appendix E: Field Collection Records for August 00 Fingerling Survival Monitoring 6

40 MNR Office Use: Watershed: Waterbody Name: Fish Dot No.: Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Telephone: Fax: 7-76 Mailing Address: 0 Bloomington Road West Town/City Aurora Postal Code: L4G 0L8 Waterbody Name: Main Upper Humber Township/Municipality: Adjala General Description of Sampling Site Location/Access: Chicco s; Collection Site No. of Site UTM Coordinates: 7T 90467E Collection Date: 0/09/0 Start Time: 9:4 End Time: 0:0 Duration (hrs) 0.7 Electrofisher Seconds: 48 Length of Station (m) 00 Water Temp. (C) 6 Air Temp. (C) 4 Stream Type: Intermittent Permanent N Watercress Present: Yes No Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Percent: Rock 0 Boulder 0 Rubble 8 Gravel 0 (Total = 00%) Silt Clay Muck Marl Other (Description) Current: Still X Slow Medium Fast Quantitative (m/s) Water Colour/Clarity: X Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Aquatic Vegetation: Cover (Shore): Cover (In Water): Gear: Sand 0 Detritus Submergent Sparse Sparse Floating Emergent Moderate Dense Moderate Other Dense Other Seine Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Trap Net Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Mesh Size (cm) Smallest: Largest: No Catch * Record released fish on back. Date: Day 0 Month 09 Year 00 Collectors: C. Pritchard, M. Pahor, T. McCartney Additional Data: (Pollution, Condition of Fish, Habitat Conditions) Continued on Reverse 7

41 Species Captured Species Brown Trout Brown Trout Brown Trout YOY Brook Trout Brook Trout Johnny Darter Central Mudminnow Blacknose Dace Creek Chub White Sucker Mottled Sculpin American Brook Lamprey Code No. Caught Identified By: C. Pritchard, M. Pahor, T. McCartney Wt (g) Size Range (T.L. in mm) Date: 0/09/0 Station Diagram (Include a map that illustrates clearly, at an appropriate scale, the location of each collection site and a diagram that illustrates the features of that site. Sites where no specimens were caught must also be included. N Hwy 0 0 0m Hwy 9 8

42 MNR Office Use: Watershed: Waterbody Name: Fish Dot No.: Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Telephone: Fax: 7-76 Mailing Address: 0 Bloomington Road West Town/City Aurora Postal Code: L4G 0L8 Waterbody Name: Main Upper Humber Township/Municipality: Caledon General Description of Sampling Site Location/Access: Humber Station; off of Humber Station Road, ending at the confluence with Hopeful Creek. Collection Site No. of Site UTM Coordinates: 7T 977E Collection Date: /08/0 Start Time: :0 End Time: :00 Duration (hrs) 0. Electrofisher Seconds: 94 Length of Station (m) 00 Water Temp. (C) 6 Air Temp. (C) 0 Stream Type: Intermittent Permanent N Watercress Present: Yes No Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Percent: Rock Boulder 0 Rubble Gravel 0 (Total = 00%) Silt Clay Muck Marl Other (Description) Current: Still Slow Medium Fast Quantitative (m/s) Water Colour/Clarity: X Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Aquatic Vegetation: Cover (Shore): Cover (In Water): Gear: Sand Detritus Submergent Floating Sparse X Moderate Sparse Moderate Emergent Dense Other Dense Other Seine Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Trap Net Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Mesh Size (cm) Smallest: Largest: No Catch * Record released fish on back. Date: Day Month 08 Year 00 Collectors: C. Pritchard, P. Davis, M. Pahor, J. Miller, T. McCartney, J. Bishop Additional Data: (Pollution, Condition of Fish, Habitat Conditions) Continued on Reverse 9

43 Species Captured Species Code Brown Trout Brown Trout Brown Trout YOY Brown Trout Brown Trout YOY Creek Chub Blacknose Dace Common Shiner White Sucker Northern Hog Sucker Longnose Dace Johnny Darter No. Caught Identified By: C. Pritchard, P. Davis, M. Pahor, J. Miller Wt (g) Size Range (T.L. in mm) Date: /08/0 Station Diagram (Include a map that illustrates clearly, at an appropriate scale, the location of each collection site and a diagram that illustrates the features of that site. Sites where no specimens were caught must also be included. N Castlederg Sdrd Humber Station Rd 00 40

44 MNR Office Use: Watershed: Waterbody Name: Fish Dot No.: Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Telephone: Fax: 7-76 Mailing Address: 0 Bloomington Road West Town/City Aurora Postal Code: L4G 0L8 Waterbody Name: Main Upper Humber Township/Municipality: Adjala General Description of Sampling Site Location/Access: Castlederg; upstream of Castlederg Bridge Collection Site No. of Site UTM Coordinates: 7T 969E Collection Date: /09/0 Start Time: :40 End Time: :0 Duration (hrs) 0. Electrofisher Seconds: 904 Length of Station (m) 00 Water Temp. (C) Air Temp. (C) Stream Type: Intermittent Permanent N Watercress Present: Yes No Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Percent: Rock Boulder Rubble Gravel 0 (Total = 00%) Silt Clay Muck Marl Other (Description) Current: Still Slow Medium Fast Quantitative (m/s) Water Colour/Clarity: X Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Aquatic Vegetation: Cover (Shore): Cover (In Water): Gear: Sand 0 Detritus Submergent Floating X Sparse Moderate Sparse Moderate Emergent Dense Other Dense Other Seine Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Trap Net Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Smallest: Mesh Size (cm) Largest: No Catch * Record released fish on back. Date: Day Month 08 Year 00 Collectors: C. Pritchard, P. Davis, J. Miller, M. Pahor, T. McCartney Additional Data: (Pollution, Condition of Fish, Habitat Conditions) Continued on Reverse 4

45 Species Captured Species Code Brown Trout No. Caught Longnose Dace White Sucker Blacknose Dace Common Shiner Creek Chub Johnny Darter 8 8 Identified By: C. Pritchard, P. Davis, J. Miller, M. Pahor, T. McCartney Wt (g) < Size Range (T.L. in mm) Date: /08/0 Station Diagram (Include a map that illustrates clearly, at an appropriate scale, the location of each collection site and a diagram that illustrates the features of that site. Sites where no specimens were caught must also be included. N Duffy s Lane 00m Castlederg Sdrd 4

46 MNR Office Use: Watershed: Waterbody Name: Fish Dot No.: Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Telephone: Fax: 7-76 Mailing Address: 0 Bloomington Road West Town/City Aurora Postal Code: L4G 0L8 Waterbody Name: Coffey Creek Township/Municipality: Caledon General Description of Sampling Site Location/Access: Markoff Property; starting approximately m downstream of footbridge Collection Site No. 4 of Site UTM Coordinates: 7T 998E Collection Date: 0/09/0 Start Time: 9:4 End Time: 0:0 Duration (hrs) 0.7 Electrofisher Seconds: 8 Length of Station (m) 00 Water Temp. (C) Air Temp. (C) 4 Stream Type: Intermittent Permanent 4866N Watercress Present: X Yes No Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Percent: Rock Boulder Rubble Gravel 40 (Total = 00%) Silt Clay Muck Marl Other (Description) Current: Still Slow Medium Fast Quantitative (m/s) Water Colour/Clarity: X Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Aquatic Vegetation: Cover (Shore): Cover (In Water): Gear: Sand 40 Detritus Submergent Floating Sparse X Emergent Moderate Sparse Moderate Dense Other Dense Other Seine Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Trap Net Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Mesh Size (cm) Smallest: Largest: No Catch * Record released fish on back. Date: Day 0 Month 09 Year 00 Collectors: C. Prichard, P. Davis, H. Lotimer, J. Miller, M. Pahor, T. McCartney Additional Data: (Pollution, Condition of Fish, Habitat Conditions) Beaver dam approximately 0m into reach; less fish caught upstream of dam Continued on Reverse 4

47 Species Captured Species Brown Trout Brook Trout Creek Chub White Sucker Mottled Sculpin Northern Redbelly Dace Blacknose Dace Longnose Dace Code No. Caught Identified By: C. Pritchard, P. Davis, H. Lotimer, J. Miller, M. Pahor, T. McCartney Wt (g) Size Range (T.L. in mm) Date: 0/09/0 Station Diagram (Include a map that illustrates clearly, at an appropriate scale, the location of each collection site and a diagram that illustrates the features of that site. Sites where no specimens were caught must also be included. N Humber Station Rd 00m Patterson Sdrd 44

48 MNR Office Use: Watershed: Waterbody Name: Fish Dot No.: Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Telephone: Fax: 7-76 Mailing Address: 0 Bloomington Road West Town/City Aurora Postal Code: L4G 0L8 Waterbody Name: Coffey Creek Township/Municipality: Caledon General Description of Sampling Site Location/Access: Coffey Creek Farm, downstream site Collection Site No. of Site UTM Coordinates: 7T 89E Collection Date: 07/09/0 Start Time: 9:4 End Time: 0:0 Duration (hrs) 0.7 Electrofisher Seconds: 08 Length of Station (m) 00 Water Temp. (C) 9 Air Temp. (C) 4 Stream Type: Intermittent Permanent 48664N Watercress Present: X Yes No Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Percent: Rock 0 Boulder Rubble Gravel 0 (Total = 00%) Silt Clay Muck Marl Other (Description) Current: Still Slow Medium X Fast Quantitative (m/s) Water Colour/Clarity: Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Aquatic Vegetation: Cover (Shore): Cover (In Water): Gear: Sand 0 Detritus Submergent Sparse Sparse Floating Emergent Moderate Dense X Moderate Other Dense Other Seine Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Trap Net Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Mesh Size (cm) Smallest: Largest: No Catch * Record released fish on back. Date: Day 07 Month 09 Year 00 Collectors: C. Pritchard, M. Pahor, J. Miller Additional Data: (Pollution, Condition of Fish, Habitat Conditions) Scale battery died while processing fish. Missing bulk weights for some species. Continued on Reverse 4

49 Species Captured Species Atlantic Salmon Brown Trout Brook Trout Blacknose Dace Creek Chub Mottled Sculpin Longnose Dace White Sucker Identified By: C. Pritchard, M. Pahor, J. Miller Code No. Caught Wt (g) Size Range (T.L. in mm) Date: 07/09/0 0 Station Diagram (Include a map that illustrates clearly, at an appropriate scale, the location of each collection site and a diagram that illustrates the features of that site. Sites where no specimens were caught must also be included. N Finnerty Sdrd 00m Gore Rd 46

50 MNR Office Use: Watershed: Waterbody Name: Fish Dot No.: Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Telephone: Fax: 7-76 Mailing Address: 0 Bloomington Road West Town/City Aurora Postal Code: L4G 0L8 Waterbody Name: Coffey Creek Township/Municipality: Caledon General Description of Sampling Site Location/Access: Coffey Creek Farm, upstream site; reach starts 0m downstream of footbridge Collection Site No. 6 of Site UTM Coordinates: 7T Collection Date: 07/09/0 Start Time: :4 End Time: :00 Duration (hrs) 0.7 Electrofisher Seconds: Length of Station (m) 00 Water Temp. (C) Air Temp. (C) Stream Type: Intermittent Permanent 8908E 486N Watercress Present: Yes No Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Percent: Rock Boulder Rubble Gravel 0 (Total = 00%) Silt Clay Muck Marl Other (Description) Current: Still X Slow Medium Fast Quantitative (m/s) Water Colour/Clarity: X Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Aquatic Vegetation: Cover (Shore): Cover (In Water): Gear: Sand 0 Detritus Submergent Floating Sparse X Moderate Sparse X Moderate Emergent Dense X Other Dense Other Seine Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Trap Net Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Mesh Size (cm) Smallest: Largest: No Catch * Record released fish on back. Date: Day 07 Month 09 Year 00 Collectors: C. Pritchard, P. Davis, J. Miller, M. Pahor Additional Data: (Pollution, Condition of Fish, Habitat Conditions) Continued on Reverse 47

51 Species Captured Species Code No. Caught Brown Trout Brook Trout Mottled Sculpin 8 Longnose Dace Creek Chub Blacknose Dace 0 White Sucker 6 Identified By: C. Pritchard, P. Davis, J. Miller, M. Pahor 7 Wt (g) Size Range (T.L. in mm) Date: 07/09/0 Station Diagram (Include a map that illustrates clearly, at an appropriate scale, the location of each collection site and a diagram that illustrates the features of that site. Sites where no specimens were caught must also be included. N Finnerty Sdrd 00m Gore Rd 48

52 MNR Office Use: Watershed: Waterbody Name: Fish Dot No.: Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Telephone: Fax: 7-76 Mailing Address: 0 Bloomington Road West Town/City Aurora Postal Code: L4G 0L8 Waterbody Name: Coffey Creek Township/Municipality: Caledon General Description of Sampling Site Location/Access: Coffey Creek Farm Finnerty branch; reach ends at Finnerty Sideroad Collection Site No. 7 of Site UTM Coordinates: 7T 8900E Collection Date: 07/09/0 Start Time: :4 End Time: : Duration (hrs) 0. Electrofisher Seconds: 66 Length of Station (m) 0 Water Temp. (C) 0 Air Temp. (C) Stream Type: Intermittent Permanent 486N Watercress Present: Yes No Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Percent: Rock 0 Boulder Rubble Gravel 0 (Total = 00%) Silt Clay Muck Marl Other (Description) Current: Still Slow X Medium Fast Quantitative (m/s) Water Colour/Clarity: X Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Aquatic Vegetation: Cover (Shore): Cover (In Water): Gear: Sand 0 Detritus Submergent Sparse Sparse Floating Emergent Moderate Dense X Moderate Other Dense Other Seine Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Trap Net Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Mesh Size (cm) Smallest: Largest: No Catch * Record released fish on back. Date: Day 07 Month 09 Year 00 Collectors: C. Pritchard, J. Miller, P. Davis, M. Pahor Additional Data: (Pollution, Condition of Fish, Habitat Conditions) Continued on Reverse 49

53 Species Captured Species Brown Trout Brook Trout Northern Redbelly Dace Creek Chub Mottled Sculpin Longnose Dace Blacknose Dace Code No. Caught Wt (g) Size Range (T.L. in mm) Also caught large brook trout, approx. 0mm, which we released before processing because he was not doing well... Identified By: C. Pritchard, J. Miller, P. Davis, M. Pahor Date: 07/09/0 Station Diagram (Include a map that illustrates clearly, at an appropriate scale, the location of each collection site and a diagram that illustrates the features of that site. Sites where no specimens were caught must also be included. Finnerty Sdrd N 0m Gore Road 0

54 MNR Office Use: Watershed: Waterbody Name: Fish Dot No.: Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Telephone: Fax: 7-76 Mailing Address: 0 Bloomington Road West Town/City Aurora Postal Code: L4G 0L8 Waterbody Name: Coffey Creek Township/Municipality: Caledon General Description of Sampling Site Location/Access: Fimes Property, downstream site; starts at Innis Lake Rd Collection Site No. 8 of Site UTM Coordinates: 7T 8776E Collection Date: 4/09/0 Start Time: 0:00 End Time: 0:4 Duration (hrs) 0.7 Electrofisher Seconds: 94 Length of Station (m) 00 Water Temp. (C) 4 Air Temp. (C) 6 Stream Type: Intermittent Permanent 4864N Watercress Present: Yes No Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Percent: Rock Boulder Rubble Gravel 40 (Total = 00%) Silt Clay Muck Marl Other (Description) Current: Still Slow X Medium Fast Quantitative (m/s) Water Colour/Clarity: X Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Aquatic Vegetation: Cover (Shore): Cover (In Water): Gear: Sand 0 Detritus Submergent Floating Sparse X Moderate Sparse X Moderate Emergent Dense Other Dense Other Seine Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Trap Net Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Mesh Size (cm) Smallest: Largest: No Catch * Record released fish on back. Date: Day 4 Month 09 Year 00 Collectors: C. Pritchard, J. Miller Additional Data: (Pollution, Condition of Fish, Habitat Conditions) Continued on Reverse

55 Species Captured Species Brook Trout Code No. Caught Wt (g) < Identified By: C. Pritchard, J. Miller Size Range (T.L. in mm) Date: 4/09/0 Station Diagram Finnerty Sdrd N 00m Innis Lake Rd

56 MNR Office Use: Watershed: Waterbody Name: Fish Dot No.: Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Telephone: Fax: 7-76 Mailing Address: 0 Bloomington Road West Town/City Aurora Postal Code: L4G 0L8 Waterbody Name: Coffey Creek Township/Municipality: Caledon General Description of Sampling Site Location/Access: Fimes Property, upstream site; ends at upstream fenceline Collection Site No. 9 of Site UTM Coordinates: 7T Collection Date: 0/09/0 Start Time: :00 End Time: :0 Duration (hrs) 0. Electrofisher Seconds: 607 Length of Station (m) 00 Water Temp. (C) Air Temp. (C) 7 Stream Type: Intermittent Permanent 87464E N Watercress Present: Yes No Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Percent: Rock 0 Boulder Rubble Gravel 0 (Total = 00%) Silt Clay Muck Marl Other (Description) Current: Still X Slow Medium Fast Quantitative (m/s) Water Colour/Clarity: X Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Aquatic Vegetation: Cover (Shore): Cover (In Water): Gear: Sand 0 Detritus Submergent Sparse Sparse Floating Emergent X Moderate Dense Other Moderate Dense Other Seine Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Trap Net Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Mesh Size (cm) Smallest: Largest: No Catch * Record released fish on back. Date: Day 0 Month 09 Year 00 Collectors: C. Pritchard, M. Pahor, T. McCartney Additional Data: (Pollution, Condition of Fish, Habitat Conditions) Continued on Reverse

57 Species Captured Species Brook Trout Atlantic Salmon Identified By: C. Pritchard, M. Pahor, T. McCartney Code No. Caught 77 Wt (g) Size Range (T.L. in mm) Date: 0/09/0 Station Diagram Finnerty Sdrd N 00m Innis Lake Rd 4

58 Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Telephone: Fax: 7-76 Mailing Address: 0 Bloomington Road West Town/City Aurora Postal Code: L4G 0L8 Waterbody Name: Coffey Creek Township/Municipality: Caledon General Description of Sampling Site Location/Access: Stewart Property by-pass channel; ends at outlet of pond Collection Site No. 0 of Site UTM Coordinates: 7T 87066E Collection Date: /0/09 Start Time: :4 End Time: :0 Duration (hrs) 0. Electrofisher Seconds: 47 Length of Station (m) 00 Water Temp. (C) Air Temp. (C) 8 Stream Type: Intermittent Permanent 48684N Watercress Present: X Yes No Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Percent: Rock 7 Boulder Rubble Gravel (Total = 00%) Silt 0 Clay Muck Marl Other (Description) Current: Still Slow X Medium Fast Quantitative (m/s) Water Colour/Clarity: X Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Aquatic Vegetation: Cover (Shore): Cover (In Water): Gear: Sand 0 Detritus Submergent Floating Sparse Sparse Moderate X Moderate X Emergent Dense Other Dense Other Seine Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Trap Net Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Smallest: Mesh Size (cm) Largest: No Catch * Record released fish on back. Date: Day 0 Month 09 Year 00 Collectors: C. Pritchard, M. Pahor, T. McCartney Additional Data: (Pollution, Condition of Fish, Habitat Conditions) - Low water levels Continued on Reverse

59 Species Captured Species Brook Trout Code No. Caught Wt (g) Identified By: C. Pritchard, M. Pahor, T. McCartney Size Range (T.L. in mm) Date: 0/09/0 Station Diagram (Include a map that illustrates clearly, at an appropriate scale, the location of each collection site and a diagram that illustrates the features of that site. Sites where no specimens were caught must also be included. N Farm Access Road 00m 6

60 MNR Office Use: Watershed: Waterbody Name: Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Mailing Address: 0 Bloomington Road West Telephone: Town/City Aurora Fax: 7-76 Postal Code: L4G 0L8 Waterbody Name: Hopeful Creek Township/Municipality: Caledon General Description of Sampling Site Location/Access: Hopeful Creek, downstream site; downstream of Humber Station Road Collection Site No. of Site UTM Coordinates: 7T Collection Date: 4/09/0 Electrofisher Seconds: Start Time: :0 Length of Station (m) 97E 48967N End Time: : Water Temp. (C) Duration (hrs) Air Temp. (C) Stream Type: Intermittent Permanent Watercress Present: Yes No Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Rock Boulder Rubble Gravel Sand 0 Percent: Silt Clay Muck Marl Detritus (Total = 00%) Other (Description) Current: Still X Slow Medium Fast Quantitative (m/s) Water Colour/Clarity: Aquatic Vegetation: X Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Submergent Cover (Shore): Cover (In Water): Gear: Seine Trap Net Floating Emergent X Sparse X Moderate Dense Other Sparse Moderate Dense Other Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Mesh Size (cm) Smallest: Largest: No Catch * Record released fish on back. Date: Day 4 Month 09 Year 00 Collectors: C. Pritchard, J. Miller Additional Data: (Pollution, Condition of Fish, Habitat Conditions) Continued on Reverse 7

61 Species Captured Species Atlantic Salmon (dead) Brown Trout Code No. Caught 8 Wt (g) < 6 < Size Range (T.L. in mm)

62 Brook Trout Blacknose Dace Creek Chub Identified By: C. Pritchard, J. Miller Date: 4/09/0 Station Diagram (Include a map that illustrates clearly, at an appropriate scale, the location of each collection site and a diagram that illustrates the features of that site. Sites where no specimens were caught must also be included. Humber St Rd N 00m 9

63 MNR Office Use: Watershed: Waterbody Name: Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Mailing Address: 0 Bloomington Road West Telephone: Town/City Aurora Fax: 7-76 Postal Code: L4G 0L8 Waterbody Name: Hopeful Creek Township/Municipality: Caledon General Description of Sampling Site Location/Access: Hopeful Creek, upstream site; starts at Humber Station Road and ends 00m upstream Collection Site No. of Collection Date: 7/09/0 Site UTM Coordinates: 7T Start Time: 0: 970E 48979N Electrofisher Seconds: 97 Stream Type: Intermittent Length of Station (m) 00 Water Temp. (C) Air Temp. (C) Permanent Watercress Present: Yes No End Time: :00 Duration (hrs) 0.7 Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Rock 0 Boulder Rubble Gravel 6 Sand 0 Percent: Silt Clay Muck Marl Detritus (Total = 00%) Other (Description) Current: Still X Slow Medium Fast Quantitative (m/s) Water Colour/Clarity: X Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Aquatic Vegetation: Submergent Cover (Shore): Cover (In Water): Gear: Seine Trap Net Floating Emergent X Sparse X Moderate Dense Other Sparse Moderate Dense Other Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Mesh Size (cm) Smallest: Largest: No Catch * Record released fish on back. Date: Day 7 Month 09 Year 00 Collectors: C. Pritchard, J. Miller Additional Data: (Pollution, Condition of Fish, Habitat Conditions) Continued on Reverse 60

64 Species Captured Species Brown Trout Creek Chub Blacknose Dace Identified By: C. Pritchard, J. Miller Code 0 No. Caught Wt (g) 4 < Size Range (T.L. in mm) Date: 7/09/0

65 Station Diagram (Include a map that illustrates clearly, at an appropriate scale, the location of each collection site and a diagram that illustrates the features of that site. Sites where no specimens were caught must also be included. Humber St Rd N 00m 6

66 MNR Office Use: Watershed: Waterbody Name: Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Mailing Address: 0 Bloomington Road West Telephone: Town/City Aurora Fax: 7-76 Postal Code: L4G 0L8 Waterbody Name: Coffey Creek Township/Municipality: Caledon General Description of Sampling Site Location/Access: Galloway Property, from the driveway of 7099 Finnerty Sdrd to the dam Collection Site No. of Collection Date: 7/08/0 Site UTM Coordinates: 7T Start Time: : 8874E 48647N Electrofisher Seconds: 7 Stream Type: Intermittent Length of Station (m) 00 Water Temp. (C) N/A Air Temp. (C) N/A Permanent Watercress Present: Yes No End Time: :00 Duration (hrs) 0.7 Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Rock 0 Boulder Rubble Gravel 70 Sand Percent: Silt Clay Muck Marl Detritus (Total = 00%) Other (Description) Current: Still Slow Medium Fast Quantitative (m/s) Water Colour/Clarity: X Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Aquatic Vegetation: Submergent Cover (Shore): Cover (In Water): Gear: Seine Trap Net Floating Emergent Sparse X Moderate Dense Other Sparse Moderate Dense Other Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Mesh Size (cm) Smallest: Largest: No Catch * Record released fish on back. Date: Day 7 Month 08 Year 00 Collectors: C. Pritchard, M. Pahor, J. Miller, T. McCartney, B. Hope, C. Hargreaves Additional Data: (Pollution, Condition of Fish, Habitat Conditions) Continued on Reverse 6

67 Species Captured Species Code No. Caught Atlantic Salmon Brook Trout Wt (g) Brown Trout Largemouth Bass Mottled Sculpin 8 9 White Sucker Longnose Dace Blacknose Dace Creek Chub Golden Shiner 94 Identified By: C. Pritchard, M. Pahor, J. Miller, T. McCartney, B. Hope, C. Hargreaves Size Range (T.L. in mm) Date: 7/08/0 Station Diagram (Include a map that illustrates clearly, at an appropriate scale, the location of each collection site and a diagram that illustrates the features of that site. Sites where no specimens were caught must also be included. N Finnerty Sdrd #7099 Centreville Creek Rd 64

68 MNR Office Use: Watershed: Waterbody Name: Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Mailing Address: 0 Bloomington Road West Telephone: Town/City Aurora Fax: 7-76 Postal Code: L4G 0L8 Waterbody Name: Main Upper Humber Township/Municipality: Adjala General Description of Sampling Site Location/Access: St Francis Center, reach ends on downstream side of dam Collection Site No. 4 of Collection Date: 4/09/00 Site UTM Coordinates: 7T Start Time: 0:00 88E N Electrofisher Seconds: Stream Type: Intermittent Length of Station (m) 98.6 Water Temp. (C) 6 Air Temp. (C) 9 Permanent Watercress Present: Yes No End Time: :0 Duration (hrs). Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Rock Boulder Rubble 6 Gravel Sand 0 Percent: Silt Clay Muck Marl Detritus (Total = 00%) Other (Description) Current: Still X Slow Medium Fast Quantitative (m/s) Water Colour/Clarity: X Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Aquatic Vegetation: Submergent Cover (Shore): Cover (In Water): Gear: Seine Trap Net Floating Sparse Sparse Moderate Moderate Emergent X Dense Dense Other Other Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Mesh Size (cm) Smallest: Largest: No Catch * Record released fish on back. Date: Day 4 Month 09 Year 00 Collectors: C. Pritchard, J. Miller Additional Data: (Pollution, Condition of Fish, Habitat Conditions) Did not fish parallel channel upstream of bridge to dam (we did not directly stock here) Continued on Reverse 6

69 Species Captured Species Atlantic Salmon Brook Trout Code No. Caught 66 Wt (g) Size Range (T.L. in mm)

70 Mottled Sculpin Creek Chub White Sucker Blacknose Dace Identified By: C. Pritchard, J. Miller Date: 4/09/00 Station Diagram (Include a map that illustrates clearly, at an appropriate scale, the location of each collection site and a diagram that illustrates the features of that site. Sites where no specimens were caught must also be included. 00m N school 67

71 MNR Office Use: Watershed: Waterbody Name: Licence to Collect Fish for Scientific Purposes Field Collection Record Licence No: 078 Licensee: Name: Doug Forder Business Name: Ontario Streams Mailing Address: 0 Bloomington Road West Telephone: Town/City Aurora Fax: 7-76 Postal Code: L4G 0L8 Waterbody Name: Cold Creek Township/Municipality: Caledon General Description of Sampling Site Location/Access: Cold Creek upstream of Concession Collection Site No. of Collection Date: /08/00 Site UTM Coordinates: 7T E 4864N Start Time: 0:00 End Time: 0:4 Electrofisher Seconds: 70 Stream Type: Intermittent Length of Station (m) 00 Water Temp. (C) 6 Air Temp. (C) Permanent Watercress Present: Yes No Duration (hrs) 0.7 Waterbody Type: Spring Canal Stream/River River/Lake Junction Flooded Area Pool Pond Lake Reservoir Muskeg/Bog Other (Describe) Bottom Type by Rock 60 Boulder Rubble Gravel 0 Sand Percent: Silt Clay 0 Muck Marl Detritus (Total = 00%) Other (Description) Current: Still Slow Medium Fast Quantitative (m/s) Water Colour/Clarity: X Colourless Yellow/Brown Blue/Green Turbid Other Secchi Depth: (m) Aquatic Vegetation: Submergent Cover (Shore): Cover (In Water): Gear: Seine Trap Net Floating Emergent X Sparse X Moderate Dense Other Sparse Moderate Dense Other Gill Net Dip Net Angled Trawl Minnow Trap Piscicide Electrofisher Surber Other Size of Net (Gill or Seine Net) Length (m): Selectivity of Sample: All Kept Size of Net or Mouth (Trap, Hoop or Trawl) (m): Kept* Some Kept* Mesh Size (cm) Smallest: Largest: No Catch * Record released fish on back. Date: Day Month 08 Year 00 Collectors: C. Pritchard, P. Davis, M. Pahor, J. Miller, J. Bishop, T. McCartney Additional Data: (Pollution, Condition of Fish, Habitat Conditions) Continued on Reverse 68