Investigation of changes of the refractive index of glass fragments caused by laser ablation
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1 Investigation of changes of the refractive index of glass fragments caused by laser ablation Dr. Stefan Becker Bundeskriminalamt, Forensic Science Institute Wiesbaden, Germany
2 Investigation of changes of the refractive index of glass fragments caused by laser ablation Starter Aim of study & previous work Main course Experimental design & results Dessert Spin off 2 August 10, 2011
3 Starter 3 August 10, 2011
4 Starter Step-by-step approach to forensic glass analysis Glass case Physical examinations/ GRIM samples distinguishable case report Chemical examinations (ICP-MS, SEM, RFA) XRF) case report 4 August 10, 2011
5 present Glass comparison case break fragment too small RI LA-ICP-MS LA-ICP-MS? RI decreasing fragment size 5 August 10, 2011
6 Does the laser ablation process alter the results of refractive index measurements of glass fragments? What are the influencing factors? mass, size, color, geometry of a glass fragment sample preparation, laser wavelength 6 August 10, 2011
7 Third attempt to tackle this issue: 1. Oral presentation at the ENFSI Annual Meeting of the European Glass Group 2001, Oslo 2. Poster presentation at the European Winter Conference on Plasma Spectrochemistry, 30 Jan-03 February 2005, Budapest, Hungary 7 August 10, 2011
8 Work Objects # of fragments Laser wavelength [nm] 2000/2001 K5 glass float glass / float glasses August 10, 2011
9 2001 BKA-K5 standard glass for RI Crown glass produced by Schott AG with high optical homogeneity Laser Ablation: 10 Hz, 266 nm Duration: 140 s Energy: 0.7 mj Crater-ø: 80 µm 9 August 10, 2011
10 August 10, 2011
11 August 10, 2011
12 August 10, 2011
13 2001 Spatial change of RI Smaller fragment: mean n D = SD 2.2E August 10, 2011
14 Results presented in 2001 Smaller fragment/ 3 crater: Reduction of RI up to 2*10-4 Spatial gradient mm scale Bigger fragment/ 2 crater: No significant change of RI 14 August 10, 2011
15 2005 Fragments of float glass sample 13_2 covering a mass range from 17 µg µg 15 August 10, 2011
16 2005 Findings 1.) Morphological changes are created at the crater region by laser ablation (RI determination is not possible) 2.) Higher variation of RI values due to laser-glass interaction 3.) Effect of RI variation decreases with higher fragment size/mass 16 August 10, 2011
17 2005 Conclusion Alteration of the RI value of small fragments may occur in the course of the laser ablation process. More investigations on the variation of the RI of fragments of different size, mass or shape and for different laser systems (266 nm, 193 nm) are needed in order to give a universally valid statement. 17 August 10, 2011
18 2010/11 Main course 18 August 10, 2011
19 2010/11 Selection of samples Name Color heated/ tempered Producer / plant thickness/ mm 5_2 Parasol grün N green No Vegla, Herzogenrath, Germany _2 Optifloat clear No Flachglas, Weiherhammer 1, Germany _23 Dom. Heated green Yes Libbey Owens Ford L.O.F., Lathrop, CA, U.S.A August 10, 2011
20 2010/11 Instrumentation Foster & Freemann GRIM III system coupled to an existing phase contrast microscope Diaplan (Leitz, Germany) with Phaco L110/0.25 lens and Mettler Toledo FP 82 HT hotstage. Ultra-microbalance scale: Mettler Toledo UMX5 Comparator 20 August 10, 2011
21 2010/11 Instrumentation Laser ablation New Wave Research UP 213 UP 193 Solid-state laser (Nd:YAG) 213 nm gas laser (excimer ArF) 193 nm 21 August 10, 2011
22 2010/11 Workflow 1. Selection of three float glasses (colors, thermal history); isolation of glass fragments of different sizes 2. Documentation of physical properties (size, mass), photography 3. Laser ablation ICP-MS analysis of glass samples according to ISO accredited method for the quantitative analysis of glass fragments 4. Photographic documentation of ablated fragments 5. Sample preparation: Cleaning (EtOH/HNO 3 ) and breaking of fragments 6. Determination of RI using Foster & Freeman GRIM III 22 August 10, 2011
23 2010/11 Selection of fragments Glass 5_2 Group A: Size length 1 mm - 2 mm Group B: Size length 0.5 mm - 1 mm Group C: 23 August 10, 2011 Size length 0.2 mm mm
24 2010/11 Selection of fragments Glass 13_2 Group A: Size length 1 mm - 2 mm Group B: Size length 0.5 mm -1 mm Group C: 24 August 10, 2011 Size length 0.2 mm -0.5 mm
25 2010/11 Selection of fragments Inclusion of additional set of six very big fragments: Glass 13_2 13_2D1 3mmx1.5mm 3.32 mg 13_2D2 2mmx1.2mm 1.86 mg 13_2D3 2mmx1.2mm 1.77 mg 13_2D4 2.1mmx1.5mm 1.50 mg 13_2D5 2.1mmx1.6mm 2.74 mg 13_2D6 1.6mmx1.0mm 2.09 mg 25 August 10, 2011
26 2010/11 Photographical documentation (millimeter scale) name 5_2 B mm maximal length untreated glass fragment ablated glass fragment 62 µg 213 mass laser wavelength [nm] 26 August 10, 2011
27 2010/11 wavelength 213 nm wavelength 193 nm 13_2 A15 1.1mm 0.099mg 13_2 A19 1.1mm 0.093mg 13_2 B7 1.2mm mg 13_2 A20 0.8mm 0.080mg 13_2 B9 1mm 0.063mg 13_2 B6 0.8mm 0.052mg 27 August 10, 2011
28 2010/11 wavelength 213 nm wavelength 193 nm 26_23 A18 1mm 0.048mg 26_23 B8 0.9mm 0.034mg 5_2 B mm 0.039mg 13_2 B14 0.7mm 0.025mg 26_23 B15 0.7mm 0.022mg 13_2 B mm 0.022mg 28 August 10, 2011
29 2010/11 Minimum size requirements for six replicate measurements with crater diameter of approx. 80 µm: 13_2 C3 22 µg 193 nm 463 µm 370 µm 29 August 10, 2011
30 2010/11 Glass 5_2 untreated n D = SD = 3* August 10, 2011
31 2010/11 5_2 A2 568 µg 213 nm untreated n D = SD = 3* August 10, 2011
32 2010/11 5_2 A µg 213 nm untreated n D = SD = 3* August 10, 2011
33 2010/11 5_2 A µg 213 nm untreated n D = SD = 3* August 10, 2011
34 2010/11 5_2 A µg 213 nm untreated n D = SD = 3* August 10, 2011
35 2010/11 5_2 A µg 213 nm untreated n D = SD = 3* August 10, 2011
36 2010/11 5_2 A µg 213 nm untreated n D = SD = 3* August 10, 2011
37 2010/11 5_2 A µg 213 nm untreated n D = SD = 3* August 10, 2011
38 2010/11 5_2 A µg 213 nm untreated n D = SD = 3* August 10, 2011
39 2010/11 5_2 A µg 193 nm untreated n D = SD = 3* August 10, 2011
40 2010/11 5_2 A µg 193 nm untreated n D = SD = 3* August 10, 2011
41 2010/11 successful measurements vs. red curve 60 Group A: Size length 1 mm - 2 mm 50 Group B: 0.5 mm - 1 mm Untreated fragments Group C: 0.2 mm 0.5 mm _2A10 13_2B2 13_2B3 13_2A1 13_2A2 13_2A3 13_2A4 13_2A5 13_2A6 13_2A7 13_2A8 13_2A9 13_2A11 13_2A12 13_2A13 13_2A14 13_2A15 13_2A16 13_2A17 13_2A18 13_2A19 13_2A20 13_2B1 13_2B5 13_2B6 13_2B7 13_2B9 13_2B14 13_2C1 13_2C3 13_2C4 successful readings red curves 41 August 10, 2011
42 2010/11 Results for 5_2 5_2 untreated n D = 1, August 10, 2011
43 2010/11 Results for 5_2 Float glass 5_2 untreated n D = 1,52323 SD = 3*10-5 RI of 16 fragments after LA 213 nm: mean n D = 1,52330 SD = 1.0 E-4 RI of 5 fragments after LA 193 nm: mean n D = 1,52336 SD = 2.9 E-4 After exclusion of outlier mean n D = 1,52325 SD = 1.0 E-4 43 August 10, 2011
44 2010/11 Results for 13_2 13_2 untreated n D = 1,51932 SD = 0, August 10, 2011
45 2010/11 Results for 13_2 13_2 untreated n D = 1,51932 SD = 0, August 10, 2011
46 2010/11 Results for 13_2 Float glass 13_2 untreated n D = 1,51932 SD = 4*10-5 RI of 19 fragments after LA 213 nm have been determined mean n D = 1,51931 SD = 4 E-5 RI of 15 fragments after LA 193 nm have been determined mean n D = 1,51936 SD = 8 E-5 (two outlier mean n D = 1,51933 SD = 2 E-5) 46 August 10, 2011
47 2010/11 Results for 26_23 26_23 untreated n D = 1,51847 SD = 0, August 10, 2011
48 2010/11 Results for glass 26_23 Float glass 26_23 untreated n D = 1,51847 SD = 6*10-5 RI of 10 fragments after LA 213 nm: mean n D = 1,51854 SD = 1.3 E-4 RI of 6 fragments after LA 193 nm: mean n D = 1,51958 SD = 7 E-5 48 August 10, 2011
49 2010/11 Summary 1 / 2 Ablation of small glass fragments down to the size of 0.4 mm x 0.4 mm were investigated. Laser ablation leads to an increased number of invalid refractive index readings (red curve). Decreased number of successful measurements (readings) possible. 49 August 10, 2011
50 2010/11 Summary 2 / 2 No effects of different cleaning solvents (EtOH vs. HNO 3 ) No improvements of RI results by 193 nm LA Very small to negligible effects on RI for fragments > 250 µg Higher variations of individual RI and SD, but no significant changes of overall mean; RI of overall mean is correct. 50 August 10, 2011
51 2010/11 Suggestions As many as possible readings should be carried out on as many edges as possible (averaging effect) Results should be assessed with caution (higher variations of RI) Careful inspection of RI readings (outlier test) Match criteria should be applied with extreme caution 51 August 10, 2011
52 2010/11 Acknowledgments Many thanks to: Carolin Marc Dücking, Peter Watzke & Peter Weis Photographic documentation, weighing, RI measurements Laser Ablation-ICPMS measurements 52 August 10, 2011
53 2010/11 Dessert ice cream cone maximum length 200 µm 53 August 10, 2011
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