health hazards in the animal facility

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1 health hazards in the animal facility aurora brønstad allergy to fur-bearing animals bites needels/syringes exposure for drugs and chemicals used in research (urine, faces or any excrete from the animal) anesthesia gas social factors reactions from friends and family restrictions on contact with animals in spare time

2 allergy proteins in urine and salvia from the animals epithelia large concentration in the bedding in the animal cages esp from old males allergy Up to 40% of people working with fur bearing lab animals develop allergy within 1-2 years (literature study) symptoms: running eyes and/or nose skin reaction severe cases: asthma

3 Monitoring program at UoB UoB has a program for all new employees who work with animals (> 1 h/week or > 40 hours/year) This is organized by the company health service ( Bedriftshelsetjeneten ) and includes consultation with the company doctor and nurse lung-function testing analysis of IgE in blood samples consultations program before you get in contact with the animals after 1/2 year then after 1 and 2 years

4 information to all new users last page in the folder you find more information contact BHT on or folder can be downloaded from English Norwegian brukerinf.pdf

5 ivc cages air-shower & laminar air flow cabins

6 dress code change coat and shoes is mandatory when enter the animal facility change pants and shirt every time you work directly with animals use gloves, face mask and hood you find clothes in the wardrobe take a shower before you go home family members can become allergic to!! Gas Anesthesia

7 Arbeid med gassanestesi og kontroll av apparater for gassanestesi til dyr ved... institutt/avd. (routines for work with anesthesia machines at UoB) Aim: Make sure that employees and students are not exposed to anesthetic gases Special care should be taken to pregnant and breast feeding women Exposure to Anesthetic Gas failure on anesthetic equipment leakage through tubes and connections user failure ignorance of routine maintenance minimal knowledge about anesthetic equipment

8 anesthesia Isoflurane, Sevoflurane N2O ( Lystgass ) anesthesia mask gas inn gas out

9 Anesthesia machine Anesthesia Machine 2

10 Anesthesia machine 3 univentor 400 Tubes and connections

11 anesthesia chamber

12 Flowmeter flow ml/kg/min tube, mask, chamber Vaporizer Anesthesia gas % Isoflurane, sevoflurane LUNGS vehicle gas under pressure Oxygen + N2O or medical air BLOOD Reduce brain activity produce unconsciousness anesthesia user failure ignorance of maintenance ignorance of leakage testing pressure test sniffer test soap test

13 Leakage control of equipment Visual control Leakage test put pressure on the system beware of pressure drop - SHOULD BE STABLE FOR search systematically for defect soap test - GAS BUBBLES at local leakage Special sniffer to detect leakage of anesthesia gas (NEW) sniffer

14 all anesthesia machines Unique id Unique maintenance protocol/check list Owner of equipment Whom to report failure Maintenance pressure test before start regularly check tubes and connection replace defect items simple setups - avoid to many connections mark/identifying different items Service and maintenance log regularly check of vaporizer

15 Use ventilation bench - do not cover point ventilation Do not sit close to equipment when not necessary Pregnant women should not work with gas anesthesia Responsibility Institutes/departments implementation of procedures Owner of equipment maintenance on equipment (service log) Have manuals available for users training of personnel Users responsibility Check equipment before use

16 Know some potential causes for health hazards when working in an animal facility Understand the importance of personal protection and compliance to regulations in animal facility Allergy Sources of allergens Risk procedures Protection and preventive actions Monitoring development of allergy at University of Bergen (and hospital) AIM - sum up Gas anesthesia Regulations for work with gas anesthesia at University of Bergen Importance of equipment maintenance (leakage, hardware failure) Importance of knowledge of correct use of anesthesia equipment (avoid user failure) Procedures for testing equipment and reporting failure The responsibility for University, Owner and user of equipment questions?

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