SHIP 2008 Submission Department of Surgery. Aseptic Techniques in Sanitation and Surface Cleaning

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1 Using GloGerm to Assess Aseptic Techniques in Sanitation and Surface Cleaning

2 Using the Spill Leader Kit Video Develop Department Specific Training Tools Proper Handwashing Techniques

3 SHIP 2008 Submission

4 Lack of proper p basic training Lack of properly written guidelines Sloppy core laboratory techniques

5 Produce a short training video using GloGerm as a visual tool Lab Safety Induction of new personnel -Video training material is mandatory -Documented D t d in Lab Personnel Safety Induction Checklist The GloGerm Kit Update SOPs - Update Tissue culture SOPs and - Provide a SOP for liquid pipetting and bench cleaning as a reference for other experimental SOPs

6 Since 1968, Glo Germ has provided our customers with a fun and safe product that casts a revealing glow when exposed to Glo Germ's U.V. light, turning the invisible into germs you can see! Plastic simulated germs GloGerm provides a Illuminates upon exposure to UV lamp useful visualization guide The GloGerm for G effective kit includes execution of a bottle of liquid GloGerm A bottle handwashing, of GloGerm powder pipetting Portable and U.V. surface lamp cleaning techniques Some uses (indicated in website) : 1. Handwashing 2. Surface cleaning 3. Tracking the trail of germs

7 Produce a short training video using GloGerm as a visual tool The GloGerm Kit

8 GloGerm Kit MSDS Small patch test on demonstrator s skin to test for irritation

9 1. Handwashing video Proper and improper handwashing techniques 2. Aseptic techniques in pipetting and surface cleaning video Decanting vs liquid removal with vacuum pump unit Proper and sloppy liquid transfer with a pipette p aid and micropipette Proper and improper bench cleaning

10 Aseptic techniques in pipetting and surface cleaning video: SHIP 2008 Submission

11 Lab Safety Induction of new personnel -Video training i material is mandatory -Documented in Lab Personnel Safety Induction Checklist

12 Videos are available online on IVLE for all Surgery staff and students. t

13 Safety Induction Procedures for new staff and students SHIP 2008 Submission All new staff and students For students/personnel ts/pe so e below 21 * Required to fill up Facility Access Exclusion of liability and Indemnity Form * * 1) Lab Supervisor or PI to guide and inform staff/student regarding the safe practices of the lab using the Personnel Safety Checklist Form 2) New staff/student to perform Lab bsafety Assessment on NUS IVLE. Contact Rachel (surtsm@nus.edu.sg) on details for IVLE access. 3) Undergo OSHE Structured Safety Training System. For staff, register for the relevant courses with OHR or through the Training option in mynus staff Portal. For students, register online at OSHE s website: ( Lab specific safety orientation Department specific training NUS OSHE managed safety courses * Upon completion, send a copy of these forms and Lab Assessment results to Juwita at or scan and to surjnr@nus.edu.sg For staff, students and personnel who are on short term projects, this (3) may not be possible due to time constraints. However, (1) and (2) are mandatory.

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15 Update SOPs Tissue culture SOP Pipetting and bench cleaning SOP

16

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18 Safer lab environment Facilitates core skills training and enhance written SOPs Fun and convenient tools for safety training

19 Thank you! Team Biopolice

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