Sequence of Basic Job Steps Potential Hazards Controls & Recommended Actions

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1 Job Hazard Analysis and Mitigation page 1 of 11 Task or Employee: Mitchell Miller Routine Non-routine Retention: Completed Routine JHAMs are retained by the employee and supervisor. Non-routine JHAMs are retained until the task is fully closed out. In the case of an accident, the form is to be retained for use by the review team. Complete instructions and supporting information is available at Enter information into boxes which will expand to accommodate whatever length of text is entered. Once this JHA is complete, all participants should sign in the Acknowledgement section. Add rows by placing cursor in the right box of the last row and entering a tab. Computers Eye strain Ergonomic problems: carpal tunnel and back problems Entering and working in research areas / shops / experimental facilities / unfamiliar areas / areas with changing hazards Exposed to all of the work activities that are being performed in area such as: Moving machinery Trips Flying chips/metal Weld flash Crane hazards Electrical hazards Vacuum Hand and power tools Low power lasers (Class I and IIa) Not all hazards may be Ergonomic evaluation (contact Medical Department 2281) and follow through with recommendations. Ergonomic computers supplies can be ordered. Take frequent breaks/stretch/move (suggest every 15 minutes) Your monitor refresh rate should be at least 70Hz for CRT Training Ergonomics (course 291) Observe and follow safety signs (for instance, wear safety glasses in this area, close-toes shoes required hard hat area ) Establish and respect barricades and postings. Observe safety protocols when working with specific equipment Do not watch welding arc Be aware of your surroundings Verify working condition of tools, use appropriate PPE, use tool for the purpose it was designed Do not overload circuits especially for bake-outs Do not look into laser beam or point at others obey safety labels on lasers Respect alarms

2 Job Hazard Analysis and Mitigation Mitchell Miller page 2 of 11 obviously identified. Don t touch stuff unless you are sure it is o.k. to do so Contact area manager if unsure of hazards. Reach high shelves/move furniture. Fall hazard Items falling on you Strain from reaching Put heavy items on lower shelves Use proper ladder/stepping stool Secure heavy items to wall Trainig Stairways and Ladders (course 293) Driving vehicles Accidents Maintain current Driver's License Follow all traffic rules Use defensive driving techniques Report problems with government vehicles When you can, walk / ride a bike instead of diving a car Wear appropriate PPE when driving a scooter Wear helmet when riding bike (suggested) Driving rules apply to cart, scooter and bike usage Work with electronic equipment (computers, lights, appliances, power supplies-both low and high voltage, test equipment) Shock hazard Use equipment only according to manufacturer's instructions Don t daisy chain power cords Don t or overload circuits (limit items plugged into power strips to 5 Amps or less) Have damaged power cords replaced. Use only approved space heaters Training Electrical Safety R&D Equipment (course 251); Also, consult SLAC ES&H Manual, Chapter 8: Electrical Safety

3 Job Hazard Analysis and Mitigation Mitchell Miller page 3 of 11 Laser pointer Eye damage from direct exposure Do not look into laser beam or point at others obey safety labels on lasers Use of hand tools (non-powered) such as screw drivers, wrenches, wire cutters, cutting with scissors, knives etc Cut hands/knuckles Stab wound Eye injury Use the right tool for the job Follow proper procedures Wear gloves when practical Wear safety glasses if something may fly into your eye. Use liquid wench or similar material to pre-loosen tight bolts. Apply force away from your body when prying with hand-tools (like a screwdriver) Keep tools sharp Lock blades down when finished Report defective equipment Slip, trip, fall, stair Fall Eyes on path Don t rush Use handrails Clean spills when you see them Exercise care when opening doors into passages Don t carry too much Earthquake Objects falling on you. Objects obstructing exit path Do not store heavy objects above your head in your primary work spot. Make sure file cabinets and shelves are secured to wall / floor. ( M$FIX to have hardware secured.) Read and be familiar with Facility Emergency Plan for your work area.

4 Job Hazard Analysis and Mitigation Mitchell Miller page 4 of 11 Lifting heavy objects Muscle strains Back injuries Dispensing liquid nitrogen into tanks via a closed-loop transfer line: opening and closing valves on liquid nitrogen tanks, holding, manipulating and connecting transfer lines. Dispensing liquid nitrogen into dewars via an open flow dispensing line: opening and closing valves, holding, manipulating liquid nitrogen dispensing lines. Metal parts in contact with liquid nitrogen (dewars and transfer lines) become extremely cold and can cause burns if contacted with skin. Coupling leaks. Water from melting ice accumulating around large nitrogen tanks can be a slipping hazard. It can also become an electrical hazard if it drips on energized equipment or cables. If couplings that are frozen are forced with a wrench, a joint may break, releasing liquid nitrogen. Loud Noise Liquid nitrogen can splash into eyes or onto skin which can result in eye damage, burns or frostbite. Fabric can absorb liquid nitrogen and freeze also causing burns or frostbite. Metal parts in contact with liquid nitrogen (dewars and transfer lines) become Do not exceed your limits. Ask for help lifting or use mechanical aid. Use proper lifting technique to minimize strains and torques. Inspect equipment prior to use. Do not use equipment that appears to be unsafe. Inspect for water puddles mop up before using equipment. Safety glasses and cryogenic gloves shall be worn when opening and closing liquid nitrogen valves and contacting cold transfer lines (thin cotton and latex gloves do NOT offer enough protection against cold metal burns and may cause a burn if frozen). If the system is not equipped with an auto-shutoff system, the dewar shall not be left unattended. Verify that the couplings are not leaking at start of fill. Dewar couplings should never be forced. A heat gun should be used to warm couplings to room temperature before removing. Wear ear protection in situations when loud noise occurs. Inspect equipment prior to use. Do not use equipment that appears to be unsafe. Inspect for water puddles mop up before using equipment. Safety goggles or face shield and cryogenic gloves shall be worn (thin cotton and latex gloves do NOT offer enough protection against cold metal burns and may cause a burn if frozen). Closed-toe shoes and leg protection (e.g. long pants without cuffs or apron) are also required. Use a phase separator if available. Do not immerse the

5 Job Hazard Analysis and Mitigation Mitchell Miller page 5 of 11 extremely cold and can cause burns if contacted with skin. Water from melting ice accumulating around nitrogen tanks can be a slipping hazard. It can increase likelihood of an electric hazard when dripping on electric equipment or cables. separator in liquid nitrogen Open the valve on filling tanks slowly and adjust it to avoid excessive splashing or movement of the transfer line. Do not stand close to dewar/container while filling. Close the supply valve immediately if nitrogen begins overflowing out of the container. The dewar shall not be left unattended while filling. Working with liquid nitrogen in enclosed areas. Pouring liquid nitrogen from one dewar into another dewar or device (robot system, detector, etc.). One volume of liquid nitrogen will expand to produce ~700 equivalent volumes of gas, potentially decreasing the concentration of oxygen. Oxygen concentrations of less than 19.5 % may cause dizziness, drowsiness, decreased mental alertness, loss of consciousness and death. Oxygen deficiency in enclosed rooms. Splashing liquid nitrogen can cause eye damage or skin burns. Fabric can absorb liquid nitrogen and freeze, which can For enclosed rooms or hutches equipped with an oxygen deficiency alarm: minimize the amount of liquid nitrogen brought into the room. If the alarm sounds, evacuate the enclosed area immediately. Do not enter the area until the alarm stops. For enclosed rooms that do not have an oxygen alarm (e.g. a cold room): only rooms designated for nitrogen use can be used for liquid nitrogen work, where the maximum amount of liquid nitrogen that can be brought into the room has been posted on the door by the SSRL safety officer. If there is no posting, liquid nitrogen can not be brought into the room for use. However, closed containers of liquid nitrogen can be transported in the building elevator. Leave any enclosed room immediately if you feel dizzy or your vision starts getting blurred. Remove all liquid nitrogen when work is finished. For working in enclosed rooms, follow controls and actions described above. While pouring, safety glasses and cryogenic gloves are required (thin cotton and latex gloves do NOT offer enough protection against cold burns and may cause a burn if frozen). Closed-toe shoes and leg protection (e.g.

6 Job Hazard Analysis and Mitigation Mitchell Miller page 6 of 11 result in burns or frostbite. long pants without cuffs or apron) is also required. If a large amount of liquid nitrogen is poured on your clothes, move to a dry area and quickly remove any frozen clothing. Storing or removing items and canisters from a dewar at liquid nitrogen temperature. Transporting dewars containing liquid nitrogen Objects stored in liquid nitrogen become extremely cold; they can cause severe burns or frostbite when removed. Canisters and other items may drip liquid nitrogen onto feet while removing them from dewars. When introducing objects into full dewars, they may displace liquid nitrogen out of the dewar. Introducing warm or room temperature items into dewars may cause rapid boil off or splashing of liquid nitrogen. When carrying open dewars there is a risk of spilling or splashing liquid nitrogen. Metal or glass dewars that are dropped may break and spill liquid nitrogen. Broken glass may cause a cut. Lifting and carrying heavy dewars may cause strain injuries. Safety glasses and cryogenic gloves are required (thin cotton and latex gloves do NOT offer enough protection against cold metal burns and may cause a burn if frozen). Closed-toe shoes and leg protection (e.g.long pants without cuffs or apron) are also required. Do not overfill storage dewars. Do not fill to the rim. Leave space to insert items and the dewar lid. Insert items slowly. Do not carry heavy dewars or tanks. Use a cart, trolley or purpose built transfer device. Ask for assistance if necessary. Dewars with handles shall have a lid in place when transported. Dewars without a handle containing more than 0.5 liter liquid nitrogen shall NOT be lifted or transported. Dewars without a handle can be carried if they contain less than 0.5 liter liquid nitrogen and when cryogenic gloves and safety glasses are used. Walk slowly when transferring liquid nitrogen containers.

7 Job Hazard Analysis and Mitigation Mitchell Miller page 7 of 11 If a liquid nitrogen container breaks, guard the area from people walking on the broken pieces. The pieces can be swept up after a few minutes once they warm up. Disposing of liquid nitrogen from open dewars: either by evaporation, gas assisted evaporation or by dispensing on the ground Manipulating samples in liquid nitrogen dewars (e.g. protein crystals, XAS cell, etc.) Warming dewars, couplings, and frozen tools to room temperature with a lowpower dryer (< 1000 W). Leaving open dewars to evaporate unattended, has a risk of spillage or accidents by persons unaware of the dangers. Pouring small amounts of liquid nitrogen on the ground to evaporate may cause splashing of liquid nitrogen. Bare hands which are required for manipulations are in close vicinity to liquid nitrogen and may be burned or suffer from frostbite. The tools used to hold and manipulate protein samples can become extremely cold. Liquid nitrogen may splash in the eyes. Open dewars may topple over and spill liquid nitrogen. Burns from hair dryer Explosion of glass dewar from overheating Only leave liquid nitrogen to evaporate in safe, well ventilated areas away from table edges. Small quantities of liquid nitrogen (< 0.5 Liter) can be evaporated by slowly pouring on the ground in a clear open area. Cryogenic gloves, safety glasses, closed-toe shoes and leg protection (e.g. long pants without cuffs or apron) are required. Large quantities of liquid nitrogen shall be disposed of by pouring slowly on the ground outdoors in a clear open area or by using a warm nitrogen gas bubbler. Cryogenic gloves, safety glasses and closed-toe shoes are required. Safety glasses are required when manipulating protein crystals in liquid nitrogen. Use only tools specifically made for manipulating samples in liquid nitrogen. Protein crystal manipulators must have handles made of Teflon or other insulating material such as nylon or rubber. If a manipulation tool is very cold to the touch, remove the tool using a cryogenic glove and let it warm up to room temperature before using with bare hands. Work on a flat, even, non slippery surface. Work away from table edges. Do not use devices producing more than 1000 W to warm/dry dewars and frozen components, only lowpower dryers. Only heat non-flammable items/materials to room

8 Job Hazard Analysis and Mitigation Mitchell Miller page 8 of 11 Leveling and calibrating components in the SAM system dewar. Using the Robot Override Key to develop the robot system, reset the robot, calibrate the robot, or give a demonstration to a tour group. Fire temperature do not overheat. Components that are being warmed shall never be left unattended. Sudden auto-filling causing splashing in liquid nitrogen dewar Objects stored in or near liquid nitrogen become extremely cold; they can cause severe burns or frostbite when contacted. By using the Robot Override Key, the robot can move while the room or hutch is occupied, potentially striking the occupants, causing bodily harm. Operate beam lines Exposure to radiation (x-ray and UV) Moving equipment (pinch/crush) Cryogenic gloves and safety glasses are required. The auto-filling device shall be disabled during leveling Reading and signing the Robot Interlock Override Procedures document is required. Carefully follow all procedures. Verify that you and others are not in the potential robot path (not just the programmed path) before each move of the robot. Keep others that do not need to be present, out of the hutch or room. Do not leave robot unattended in override mode Obtain GERT or RWT training as appropriate. No-Bars training required and protocols observed. Keep clear of moving equipment Be aware of potential pinch/crush points Follow appropriate precautions when operating electron impact x-ray source Work in noisy areas Damage to hearing Hearing protection is suggested when operating loud equipment or when in noisy area. Hearing protection is required in areas identified

9 Job Hazard Analysis and Mitigation Mitchell Miller page 9 of 11 hearing protection required or when identified as required by an industrial hygienist. Work at height/use of ladders Injury from fall, or being struck by dropped object If working at heights where falls of 4 or more feet are possible, receive harness training; wear fall protective harness. Receive ladder training, and follow given procedures. Stay out of ladder footprint when ladder is in use by another person. Operate hand power tools such as drill motors, rotary tools, etc Eye injury from flying chips, cutting fluids, or parts coming loose from machine Fingers/ hands/ jewelry/ clothing/ hair can get caught in rotating parts Exposure to high noise levels Chips/ coolant/ flying parts can hit operator or bystander Cuts Coolant on floor around machine can cause slip hazard Parts can become hot and burn skin Electrical shock Exposure to hazardous materials Repetitive motion injury Only operate equipment you that you are trained and qualified to operate. Follow all manufactures safety requirements. Limit distractions Wear safety glasses with side shields Where ear protection if exposed to prolonged high noise levels, or when posted as mandatory. Shirts and shop coats should be short sleeved. If long sleeved garments are worn, they must be tight fitting around the wrists and forearms. Keep long hair tied back. Keep necklaces and badges tucked away. Use break away badge necklaces. Inspect power cords for wear and damage. Do not use equipment with damaged cords. Use GFCI on extension cords when working outside or in wet environments. Use proper clamping and tooling. Do not exceed the capacity of the machine. Double check machine tooling and make sure chuck is removed before power is applied. Training Electrical Safety for Non-Electrical Workers (course 239) or Electrical Safety R&D Equipment (course 251);

10 Job Hazard Analysis and Mitigation Mitchell Miller page 10 of 11 Unescorted access into radiological areas. Sample preparation laboratory chemical work. Use of chemicals and other research materials including crystals, brittle/toxic samples, optics, solder & flux, alcohol and other organic solvents, lead, epoxies, paint, cutting fluids, lubricants. - Preparation of standard samples (protein crystallization, Si powder, Se powder, etc) - Working with protein crystals (including crystallization solutions, chemicals for the production of derivatives, buffers, and cryo-protectants) for producing calibration standards and for research purposes. Exposure to radiation Follow all signage Wear dosimeter Training General Employee Radiological Training (GERT) (course 115) required for unescorted access to Radiologically Controlled Areas (RCA s) Remember GERT training does not allow you to access High Radiation Areas!! Eye and skin injury Inhalation and ingestion risks Ensure proper MSDSs for all chemicals that you use are posted in the work area. Ensure proper labeling of all containers using NFPA labels Practice safe chemical handling techniques. Read information on the material safety data sheet before handling a chemical and follow recommended procedures for handling the material (eye protection, gloves, use of fume hood, etc). Use appropriate PPE and procedures for handling and disposal. Needles and sharps should be disposed of in the sharps containers provided, and glass in glass disposal containers. Never throw these materials in the regular garbage or recycling bins. Dispose of excess hazardous material safely: Follow posted procedures in the laboratories, such as using satellite waste containers for solvents; waste cabinets for temporary storage, and notification of safety officers for removal from the laboratory of properly contained waste. Do not store or eat any food or beverages in the chemical laboratories.

11 Job Hazard Analysis and Mitigation Mitchell Miller page 11 of 11 Wash hands after use.. Training Hazard Communication General Training (course 103 or 105); Laboratory User CHP Training (course 199) - a written test to demonstrate understanding of basic safety rules (administered by SSRL safety officers) is compulsory before working in the sample preparation lab; SLAC ES&H Manual, Chapter 4: Hazard Communication Acknowledgements Print Name Signature or Initialed Date Supervisor: Ashley M. Deacon July 23 rd, 2008 Mitchell Miller July 23 rd, 2008