SP-400-M1/SP-403K-M1 INSTRUCTIONS

Transcription

1 SP-400-M1/SP-403K-M1 INSTRUCTIONS PACK YOUR OWN CAPILLARIES FOR HPLC AND LC-MS revision TABLE OF CONTENT SP-400-M1 AND SP-403-M1 KIT COMPONENTS PAGE 2 COMPONENT ASSEMBLY DIAGRAM PAGE 3 COMPONENT PHOTO LIBRARY PAGE 4 GENERAL INFORMATION, SAFETY & CARE PAGE 5 ASSEMBLY & CONNECT TO PRESSURE SOURCE PAGE 6 COLUMN ASSEMBLY PAGE 8 SLURRY PREPARATION PAGE 10 ATTACHMENT OF COLUMN PAGE 10 PACKING THE COLUMN & LC-MS TIPS PAGE 12 COLUMN REMOVAL PAGE 13 PARTS AND ACCESSORIES LISTING PAGE SP-400 CUTAWAY

2 Nanobaume TM is a Trademark of Western Fluids Engineering & Mfg, LLC Inconel is a Registered Trademark of Inco Alloys International, Inc. Kel-F is a Registered Trademark of the 3M Company PEEKsil TM is a Trademark of SGE International Pty. Ltd. PEEK TM polymer is a Trademark of Victrex PLC PolySULFOETHYL A TM, PolyCAT A TM, PolyHYDROXYETHYL A TM, and PolyPROPYL A TM are trademarks of PolyLC Inc. Teflon and Tefzel are Registered Trademarks of E.I. du Pont de Nemours and Company SP-400-M1/SP-403K-M1 INSTRUCTIONS Nanobaume Kit Components Use the SP-400-M1 and SP-403K -M1 Nanobaume TM kits to pack micro and capillary columns of various lengths and of outer diameters. The compact design seals effectively at high pressure, up to 9000 psi *. The following items are included in the SP-400-M1: Western Fluids Engineering and Manufacturing, LLC PART # IMAGE SP-1333 G SP-014 L SP-01 5 M SP I SP-085 J SP E SP A SP B SP C DESCRIPTION FERRULE, PEEK, FOR 1/32 OD COLUMNS (QTY 5) STAINLESS STEEL NUT, (for SP-015 only) FERRULE, KEL-F, FOR 360 MICRON OD (QTY 5) O-RING, FFKM Perfluoroesalstomer PTFE STIR BAR, 5MM X 2MM PRESSURE CONNECTION MALE FOR 1/16" TUBERESS CAP LOCK, SS316 CAP SEAL, SS316 PACKER BODY, SS316 SP-400-M1 Nanobaume TM Assembly VL-002 SP-134 SP-130 SP-125 D H F K GLASS VIAL, 2ML, 12X32MM, BOROSILICATE FERRULE FOR 1/16" COLUMN CONNECT SS NUT FOR 1/16" COLUMN CONNECT VIAL INSERT FOR 2ML VL-002 VIAL -M1 -M1 : PART # IMAGE DESCRIPTION SP-087 SP-046 SP-050 SP-055 SP-058 VL-002X CE-005-M1 SP-110 N O P Q R D T S PRESSURE GAUGE, PSI, SS316 TUBING, ID X 1/16 OD X 10CM, SS316 (QTY 2) SHUTOFF VALVE, WITH BOTTOM VENT TUBING, ID X 1/16 OD X 5 FEET, SS316 PRESSURE GAUGE GAUGE TEE FOR 1/16 OD TUBING PK/10, includes CE-003, CE-006, plus other items, see main brochure for details TANK ADAPTER, 1/4" MALE NPT TO 1/16" TUBE SP-403K-M1 nobaume TM Startup Kit 2 * Packing limit of the pressure vessel is 9000 psig with liquid media. Use of fused silica capillary and adapters will reduce the working pressure of packer to 6000 psig.

3 COMPONENTS AND ASSEMBLY SP-050 Valve with bottom vent Cap Lock SP SP-046 SP-055 Direction of Pressure Flow SP-087 Pressure Gauge with SP-058 Gauge Tee CE-003 End Fitting retains silica bed while packing Pressure Vent (Bypass) Pressure Source Tank or Pump Nut SP-130 Ferrule SP-133 or SP-134 Cap Seal SP Stir Bar SP-085 Glass Vial VL-002 VIAL INSERT SP-125 not shown O-Ring SP-130 NUT with SP-133 or SP-134 Complete Column with CE-003 End Fittings SP Packer Body SP NOW FEMALE THREAD SP-400 Packing Unit SP-400 -M1 PARTS ASSEMBLY SP-116 SP-403K-M1 PARTS ASSEMBLY DIAGRAM 3

4 COMPONENTS OF THE SP-400-M1 AND SP-403K-M1 01 A CAP LOCK, SP D VIAL VL-002 G FERRULE FOR TUBE 1/32" SP-133 J PTFE STIR BAR SP-085 B CAP SEAL SP E PRESSURE CONNECTION SP-116 H FERRULE FOR TUBE 1/16" SP-134 K VIAL INSERT SP-125 C BODY, SP F COLUMN CONNECION SP-130 Items A-M above are included in our base unit, the SP-400-M1 Nanobaume. The SP-403K-M1 Startup Kit includes all the components of the SP-400-M1, as well as the additional packing accessories illustrated below (items N-T). I O-RING SP L M COLUMN NUT SP-014 FERRULE, 360UM TUBE SP-015 N PRESSURE GAUGE SP-087 T O P Q SS TUBE 1/16"x.020" x10cm SP-046 VALVE PRESSURE RELIEF SP-050 SS TUBE 1/16"x.020"x 5FT SP-055 CE-005-M1 COLUMN ADAPTER KIT SEE MAIN BROCHURE FOR COMPONENTS CONTAINED. 1/32" OD COLUMN END FITTINGS CE-003,2 EA 1/16" OD COLUMN END FITTINGS CE-006, 2 EA 1/32" SS SCREEN.5UM, 2 EA 1/16" SS SCREEN.5UM, 2 EA FUSED SILICA 360UM OD X 150UM ID,.5MTR PEEKSIL, 1/32", 100UM ID X 100MM LONG PK/2 PEEKSIL,1/16",150UM IDX 150MM, NOT SHOWN 2 EA FERRULE, WITH SS RING 1/32" PEEK, 2 EA FERRULE, WITH SS RING 1/16" PEEK, 2 EA MALE NUT, SS 10-32, 1 EA ADAPTER SLEEVES SET FOR F.S., 1 SET R TEE, PRESSURE GAUGE SP-058 S REGULATOR ADAPTER SP-110 4

5 1. GENERAL INFORMATION 1.1 The Nanobaume is a compact stainless steel unit designed to pack capillary columns with a silica bed at high pressure. Its unique sealing design permits rapid assembly, facilitating quick packing changeover. Simple hand tightening the pressure vessel will seal up to 9000 psig with liquid media, 3000 psig with gas. No tools are required to operate the packing unit, although a set of adjustable wrenches is useful for initial setup and column assembly. SP-400-M1 and SP-403K-M1 kits include connections for 360um, 1/32" OD, 1/16" outside diameter (OD) columns, but the NANOBaume will accept small OD column diameters using the TS-015 sleeve kit. LC-MS spray tips may also be packed. Component materials include Kel-F, PEEK, 316SS, 304SS, Borosilicate glass, ETFE, and FFKM perfluoroelastomer. The vial insert (SP-125) supplied with the SP-400-M1 will accept 2 ml (12x32mm) glass vials. Other inserts are available, (please inquire) 2 PRODUCT SAFETY AND CARE 2.1 The NANOBaume is not for use under Supercritical Fluid conditions. Do not exceed the published pressure limits (9000psig liquid/3000 psig gas). Always operate with a safety shield in place. When packing with liquid media, first flood the Nanobaume and empty column with solvent. If using the TS- 015 sleeve adapters or SP-015, SP-014 Ferrule/Nut for the capillary columns, then pressure limit is reduced to 6000psig. CAP LOCK, SP O RING, SP CAP SEAL, SP BODY, SP FIG 1 FIG 2 FIG 3 FIG Hand tighten the cap lock (SP , FIG 1) on the packer body (SP ,FIG 4) and do not overtighten, as galling may occur between the stainless steel surfaces; the O-ring seals at high pressure. Scratches, nicks or dents on the sealing surface of the Cap seal (SP , FIG 3), Nanobaume body 5

6 (SP , FIG 4) or O ring (SP , FIG 2) may impede sealing performance. As the Nanobaume is primarily 316 SS; ethers, concentrated oxidizing acids are not recommended. (i.e. Hydrochloric acid at greater than 10%) If small concentrations of aggressive solvents are used during packing, it is highly recommended that the unit be flushed with a weak solvent after use. 3. ASSEMBLY AND CONNECTION TO PRESSURE SOURCE PLEASE NOTE; THE FOLLOWING INSTRUCTIONS ARE WRITTEN TO INCLUDE COMPONENTS FROM THE SP-403K-M1 STARTUP KIT. CUSTOMERS WHO PURCHASED THE SP-400-M1 BASE UNIT MAY PURCHASE THESE PACKING ACCESSORIES SEPARATELY. 3.1 Remove the SP-400-M1 (FIG 5) and SP-403K-M1(FIG 6) components from the shipping container. Components shown on Page 4 FIG 5 FIG 6 NANOBAUME NANOBAUME ACCESSORY KIT SP-400-M1 KIT SP-403K-M1 KIT 3.2 Fit the Gauge Tee (SP-058) with the pressure gauge (SP-087). Wrap the ¼ NPT threads 3-4 times with teflon tape (TTB-50) Wrench tighten. 6.

7 FIG 7 FIG 8 FIG 9 FIG 10 Gauge tee, SP-058 Gauge,SP-087 PRESSURE FITTING, SP-116 SS TUBE, SP Place the SP-116 (FIG 9) nut and ferrule on the end of the SP-046(FIG 10) stainless steel tubing, tighten into the side port of the Nanobaume until tube is secure. The SP-116 is reusable as the ferrule does not swage permanently onto the tube. Connect the free end to the gauge tee (SP-087) and wrench tighten to secure the tube. Note: if a torque wrench is purchased, SP-162 torque to 10 Inch-pounds. If not, finger tighten the nut (Clockwise direction), then wrench tighten ¼ turn then check if leaks under pressure. If leaks occur, reduce pressure, ¼ turn more and repressurize, if leaks, repeat previous steps. Use this technique for tightening all Stainless steel nuts in this publication.) FIG 11 Pressure relief Valve with bottom drain, SP Using the second piece of SS tube (SP-046), Connect one end to the Pressure gauge Tee (SP-087), and other end to the Pressure vent valve (SP-050, FIG 11). Wrench tighten both until tubes are secure using technique described in 3.3. The assembly should look like Fig

8 FIG 12 FIG 13 FIG 14 SET UP SS TUBE, SP-055 REGULATOR ADAPTER SP Connect the uppermost port of the Vent valve (SP-050, ) to the pressure source. First use the Long piece of SS tube (SP-055, FIG 13) connect using fitting provided on the vent valve (upper port, right side of FIG 11) tighten to secure the tube. Take the SP-110, FIG 14 Pressure Regulator Adapter,1/4" male NPT end and apply teflon tape to threads wrapping 3-4 times around then thread into the port of the pressure regulator(not provided). Take the free end of the tubing and place into the other end of the pressure regulator adapter, and tighten until the tube is secure. If the HPLC pump is being used for the high pressure line, connect directly to pump using a female thread union (SP-095 not provided) 4. COLUMN ASSEMBLY 4.1 If fused silica tube will be used, cut the fused silica to length using an appropriate cutting tool. A precision cutter will ensure a square end without fragmentation of the column end (SP-019, not provided). Secure the column end fitting CE-003,( FIG 15), on the exit end of the column using an appropriate adapter sleeve,(ts-015,fig 20) with Nut, ( SP-130, FIG 22) and Ferrule,( SP-133, FIG 23). The ferrule nut- sleeve fused silica combination should look like figure 21. Tighten until fingertight and then 1/4 turn more to secure the column to the end fitting. Be sure to push the column body into the end fitting to eliminate a void between the column end and the web of the union. FIG 23.5 NUT, FERRULE ASSEMBLY SP-130/SP-133 FIG 15 FIG 16 FIG 17 FIG 18 COLUMN END, CE-003 SS FERRULE, SP-092 SS NUT, SP-102 SS UNION, SP Assemble the Column end fitting columns 1/32 OD or smaller, use the CE-003 From the CE-003 kit, 8.

9 place a 0.5um screen (SP-088, not shown) into the Micro Union (SP-089, FIG 15). Ensure that the screen is set in flat against the bottom web of the union. Place the 1/32 inch OD tube or fused silica ( adapted to 1/32 OD ) thru the nut (SP-102, FIG 17) and ferrule (SP-092, FIG 16) These are provided with the union. Insert the column body into the union and while exerting pressure on the tube into the union, tighten the nut using two wrenches. Use similar procedure for the CE-006 kit for 1/16 od column bodies, follow steps 4.2. FIG 19, FUSED SILICA WITH FIG 20,TS-015 ADAPTER SLEEVE FIG 21, PEEKSIL WITH SP-130/SP-133 FERRULE SP-015, NUT SP-014 FIG 22 FIG 23 FIG 24 FIG 25 SP-130 SS NUT SP-133 FERRULE SP-015 FERRULE 360um SP-014 SS NUT use SP-133 or SP-134 ferrule use sp-130 nut use SP-014 nut use SP-015 ferrule 4.3 The nut ferrule fused silica should look like fig 19. The sleeve on the fused silica will bring it up to an outside diameter of.032 (1/32") to fit the column end fittings (CE-003) Note; that the use of the fused silica will reduce the pressure rating of the column and packer to 6000 psig. 9.

10 4.4 LC-MS tips are inserted into the appropriate ferrule, and/ or sleeve depending on OD, and do not require end fittings as the tips are fritted already. 5. SLURRY PREPARATION 5.1 PLEASE NOTE: AS CAPILLARY PACKING IS AN EXPERIMENTAL SCIENCE, SOME TESTING WITH VARIOUS SOLVENTS OR COMBINATIONS MAY BE REQUIRED FOR OPTIMAL PACKING. 5.2 Weigh 100 mg of bulk packing material into the 2ml vial (VL-002, FIG 26) Add 1.5ml of appropriate solvent, or buffer (Hexane for normal phase, Propanol or acetonitrile for reversed phase, weak buffer for ion exchange) to the vial. Add the stir bar ( SP-085, FIG 27) FIG 26 FIG 27 FIG 28 VIAL VL-002 STIR BAR SP-085 VIAL INSERT SP Place the vial on a magnetic stir plate. Allow the slurry to stir for 5 minutes or until the stationary phase is well wetted. Once the bonded phase is wetted insert the vial into the packer body cavity using the vial insert (SP-125, FIG 28) provided to hold the vial in place 10.

11 6. ATTACHMENT OF COLUMN TO NANOBAUME FIG 29 FIG 30 FIG 31 FIG 32 FUSED SILICA 360um PEEKSIL 1/32 1/16 OD FERRULE SP-134 SP-014/SP-015 SP-133 nut ferrule assembly, FIG 33 SLEEVED FUSED SILICA nut SP-130/ ferrule SP-133 FIG 34 COLUMN ON NANOBaume nut SP-130/ ferrule SP With the correct adapter, most column or tubing with an outer diameter of 1/32inch or less can be attached to the Nanobaume(FIG 29 OR 30) using the nut,sp-130 ( FIG 22)And ferrule, SP-133 (FIG23). Larger 1/16 OD columns can be attached FIG 31, using the SP-130 (FIG 32 )nut and the Ferrule SP-134 (FIG 32) ferrule combination to the Nanobaume. In the SP-400-M1 kit, three adapter ferrules are provided. For 360 um OD fused silica use the SP-014 nut with the SP-015 ferrule. For 1/32inch OD tube use the SP- 130 nut with the SP-133 ferrule. For 1/16 inch OD tube use the SP-130 nut with the SP-134 ferrule. These connections are a convenient way to connect column bodies to the Nanobaume. If the fused silica tube that has a different OD than 360, use an adapter sleeve from the TS-015 kit and the SP-130/SP-133 combination. The adapters in the TS-015 kit will adapt fused silica columns of OD of 125 to 495 micron to a new OD of 1/32 to fit the SP-130/SP-133,( FIG 33) NOTE: USE OF FUSED SILICA WILL REDUCE THE OPERATING PRESSURE LIMIT TO 6000 PSIG 3) if not accomplished already. 6.2 Unscrew the Cap Lock (SP ,FIG 1) counterclockwise and remove the Cap Seal (SP , FIG 11.

12 FIG 34 FIG 35 COLUMN INSTALLED NANOBAUME SETUP IN CAP SEAL 6.3. Using the appropriate ferrule sized to the column OD, insert the column body open end through the ferrule and the nut (refer to section 6.1) Tighten the nut only finger tight into the threaded port of the Cap seal (SP FIG 34) column will be adjusted in the next step. 6.4 With the column in place, insert the Cap Seal onto the Nanobaume body,( FIG 33). Insure that the O ring is in place (figure FIG 2) Thread on the Cap Lock (SP , FIG 1) Hand tighten only as the O- ring under pressure forms the seal, not the fit of the cap lock and seal. DO NOT OVERTIGHTEN Push the column down into the slurry vial until it meets the bottom of the vial, then withdraw the column by 1/8 inch or far enough to clear the rotation of the stir bar at the bottom of the vial. 6.6 Tighten the Nut SP-130 (or the SP-014) to firmly secure the column in place ) Use two wrenches and the wrench flats on the Cap Seal to further tighten the nut. Overtightening the nut may embed the ferrule in the Cap seal port. If this problem occurs, take out the nut, and push the ferrule from the bottom side. Use a straight piece of 1/6 ss tube, or 1/32 Ferrule extraction tools are available. (contact the factory) 7.0 PACKING THE COLUMN and LC-MS TIPS NOTE: IF PACKING WITH LIQUID MEDIA, THE PACKING SOLVENT USED SHOULD BE THE SAME AS THAT USED TO SUSPEND THE SILICA SLURRY IN THE VIAL. IF PACKING WITH GAS, USE OF NITROGEN OR HELIUM IS RECOMMENDED. 7.1 Place the assembled Nanobaume and column behind a safety shield and onto a magnetic stir plate(not PROVIDED).The setup should approximate the setup shown ( FIG 35) Turn on the stir plate. 12.

13 With the bypass on the SP-050 (FIG 11) high pressure valve still open, slowly turn on the pressure source. The system pressure will vent through the bypass until the bypass is closed. 7.2 Slowly close off the bypass (clockwise turn) on the SP-050 valve handle to gradually increase pressure to the Nanobaume. If packing with liquid media, first flood the system and column with solvent. A small amount of air will be trapped inside the Nanobaume body and will be reduced in size by the pressure and not impact the packing process. Note: do not increase the packing pressure quickly as this might break the glass vial with differential pressure. 7.3 Set the flow rate to reach a stable pressure by adjusting the nitrogen tank regulator valve, or the liquid pressure source. As the slurry packs into the column, the pressure will rise. Take care to reduce the flow rate to ensure that the pressure limits of the end fitting, column to Nanobaume connection or the SP-400 pressure Vessel are not exceeded.. Flowrate and resulting pressure are interdependent. (More flow, higher the back pressure generated with a given bed length). 7.4 Monitor the pressure gauge (SP-087, FIG 8), watching for a stable pressure level. Although the column is packed once this stable pressure is reached, the silica bed is not yet stabilized. Allow the pressure source to maintain the column pressure for at least 60 minutes, longer is some cases up to 8 hours to reach a stable pack for longer columns. 7.5 For packing of the LC_MS Tips use gas and pack at pressure not exceeding 3000 psig. Tips pack quickly and visual confirmation is used in lieu of stable pressure reading. 8 COLUMN REMOVAL AND COMPLETION 8.1 Shut off the pressure source and relieve the system pressure by gradually opening the bypass on the SP-050 valve (counter-clockwise turn). 8.2 Remove the column from the Cap Seal, loosening the nut counterclockwise 2 full turns. Carefully remove the column from the cap seal. Note, removal of the column without equalizing pressure in all parts of the NANObaume setup, might cause the packing material to extrude from the open end of the column. Please refer to FIG 36, top column is fused silica column with sleeves, middle is the 1/32 PEEKSil column, and the bottom is a 1/16 SS body. These columns are not shown with the SS screens, as they are to small to photograph. 13.

14 FIG 36 COLUMNS READY FOR ENDS ATTACHEMENT, SCREENS NOT SHOWN 8.3 Install a suitable column end fitting onto the open end of the column (SECTION 4) (FIG 36) 8.4 The newly packed column may be attached to a pump to flush with suitable solvent to waste. The column is now complete and ready for use. NOTE: WE OFFER NO GUARANTEE REGARDING COLUMN TO COLUMN PACKING REPRODUCIBILITY, COLUMN EFFICIENCY (THEREOTICAL PLATES ACHIEVED), OR COLUMN SELECTIVITY OR TAILING FACTORS, CAPILLARY COLUMN PACKING IS AT THIS STAGE, A HIGHLY EXPERIMENTAL TECHNIQUE. WARNING; PACKING A HIGH PRESSURE CAN BE HAZARDOUS, DO NOT USE AT PRESSURE ABOVE RECOMMENDED LEVELS (9000 PSIG LIQUID, 3000 PSIG GAS) NOT FOR SFC(SUPERCRITICAL FLUID) column packing with CO2. 14.

15 PARTS AND ACCESSORIES Connection of the Nanobaume to a pressure supply source requires an adapter and several lengths of high-pressure tubing. Most of the replacement parts are found included in the kits shown in this brochure. Tubing and fittings Part Numbers Description SP-013 SP-014 SP-015 SP-116 SP-130 SP-133 SP-134 SP-046 SP-055 SP-051 Ferrule for 1/32" OD columns (use with the SP-014 nut) Nut SS, male threads, Use SP-013, SP-015 ferrules only Ferrule for 360um OD (Use with SP-014 nut only)fused silica Pressure source connection, male SS Nut SS, male threads, use SP-133, SP-134 ferrules only Adapter ferrule for 1/32 OD tube, use with SP-130 nut Adapter ferrule for 1/16" ID tube, use with SP-130 Nut. Tubing, SS 1/16" ODx.020" ID x 10cm long Tubing, SS 1/16" OD x.020 " ID, 5 ft long Adapter, 1/14" NPT to 1/16" OD tubing, for adapt pressure regulator Nanobaume Parts SP SP SP SP-058 SP-085 SP-087 SP-050 SP-125 Cap lock, SS with titanium nitride coating Cap Seal, SS "O" Ring, FFKM, perfluoroelastomer Gauge Tee, for 1/16" line and 1/4" NPT female gauge connection. Stir Bar, PTFE Coated, 5x2mm Pressure gauge, 0-10,000 psig, SS, 1/4" male NPT connection. Three way dump valve, for 10,000psig line, 1/16" inch fittings included. Vial insert, Kel-F for 2l 12x32mm flat bottom vials 15

16 SP-126 Vial insert, Kel -F for ml centrifuge vials VL-002x Glass vials, 2ml, borosilicate glass, 12x32mm, Pack of 10 vials Low pressure accessories (less than 3000 psig gas or liquid) SP-098 SP-029 SP-030 "O" Ring, Viton Pressure gauge for psig, SS, 1/4" NPT male connection PEEK green 1/16" od x.030" ID tubing 5 ft length Column End fittings Parts SP-088 SP-096 SP-089 SP-092 SP-103 SP-102 SP-095 SP-096 SP-135 SP-136 SP-137 Screen SS, 1/32" diameter with 0.5um sold each Screen SS, 1/16 diameter with 0.5um, sold each 1/32 SS micro-union including nuts and ferrules Ferrule, SS, 1/16 for SP-095 nion Ferrule, 1/32" for the SP-089 union Nut for the above SP-089 1/16" SS Micro-union including nuts and ferrules Screen, SS 1/16" micro union, 0.5um for the SP-095 SSI Ferrule 1/16" for SP-050 SSI Nut, 1/16" for High Pressure SP-050 Ferrule SS, OD 1/16" for the SP-058 Miscellaneous items SP-160 SP-161 SP-019 SP-162 SP-110 Three way SS valve 1/16 fittings, 2500psig limit Teflon tape for NPT threaded parts Precision Cutter for Fused silica Torque wrench 1/4inch Hex, 10 inch- pounds Pressure Regulator, Adapter, ¼ NPT threads to 1/16 tube, 16.

17 Kits CE-003 CE-006 CE-005 CE-007 Column end fitting kit, for 1/32 OD tube Column end fitting kit, for 1/16 OD tube Fitting kit for column adapters, 1/32 inch OD and smaller bodies Fitting kit for column adapters, 1/16 inch OD column bodies SP-400 mod 1 Nanobaume post SP-401 mod 1 Nanobaume Kit post TS-015 Adapter kits for increasing OD to 1/32 inch from fused silica OD of 125 to 495 micron Fused silica Tubing Adapters (PEEK, 6000psig rated) Catalog number Description (For Fused silica of OD listed below) SP-068 dimension) SP-069 SP-070 SP-071 SP-072 SP-073 SP-074 SP-075 SP-076 SLEEVE, PEEK, 1/32 inch OD, adapting fused silica tube of um OD (Outside SLEEVE, PEEK, 1/32 inch OD, adapting fused silica tube of um OD SLEEVE, PEEK, 1/32 inch OD, adapting fused silica tube of um OD SLEEVE, PEEK, 1/32 inch OD, adapting fused silica tube of um OD SLEEVE, PEEK, 1/32 inch OD, adapting fused silica tube of um OD SLEEVE, PEEK, 1/32 inch OD, adapting fused silica tube of um OD SLEEVE, PEEK, 1/32 inch OD, adapting fused silica tube of um OD SLEEVE, PEEK, 1/32 inch OD, adapting fused silica tube of um OD SLEEVE, PEEK, 1/32 inch OD, adapting fused silica tube of um OD PEEKSIL Empty column bodies 1/32" OD Catalog number description pressure rated SP um ID x 1/32" OD, 50mm long, pack of 2 10,000 psig SP um ID x 1/32" OD, 100mm long, pack of 2 10,000 psig SP um ID x 1/32" OD, 150mm long, pack of 2 10,000 psig SP um ID x 1/32" OD, 50mm long, pack of 2 10,000 psig 17.

18 SP um ID x 1/32" OD, 100mm long, pack of 2 10,000 psig SP um ID x 1/32" OD, 150mm long, pack of 2 10,000 psig SP um ID x 1/32" OD, 50mm long, pack of 2 10,000 psig SP um ID x 1/32" OD, 100mm long, pack of 2 10,000 psig SP um ID x 1/32" OD, 150mm long, pack of 2 10,000 psig SP um ID x 1/32" OD, 50mm long, pack of 2 10,000 psig SP um ID x 1/32" OD, 100mm long, pack of 2 10,000 psig SP um ID x 1/32" OD, 150mm long, pack of 2 10,000 psig SP um ID x 1/32" OD, 50mm long, pack of 2 8,500 psig SP um ID x 1/32" OD, 100mm long, pack of 2 8,500 psig SP um ID x 1/32" OD, 150mm long, pack of 2 8,500 psig Use CE-003 for column end fittings PEEKSIL Empty Column Bodies 1/16" OD Catalog number description pressure rated SP um ID x 1/16" OD, 150mm long, each 10,000 psig SP um ID x 1/16" OD, 200mm long, each 10,000 psig SP um ID x 1/16" OD, 150mm long, each 8,500 psig SP um ID x 1/16" OD, 200mm long, each 8,500 psig SP um ID x 1/16" OD, 150mm long, each 6,500 psig SP um ID x 1/16" OD, 200mm long, each 6,500 psig Use CE-006 for column end fittings 1/32, 1/16 OD ; Other PEEKSil ID s and Lengths are available, in precut lengths; Please inquire Bulk Bonded Phase Supports, Bulk sold by the gram SP-008 SP-004 SP-005 Strong Cation Exchange, PolySulfoethyl A 5u, 300A Weak Cation Exchange, PolyCAT A 5um, 1000A Hydrophillic Interaction, (HILIC) PolyHYDROXYethyl A, 5um, 300A 18.

19 SP-007 SP-006 SP-002 SP-001 Hydrophobic Interaction (HIC) Polypropyl A, 5um 300A C-18, 5um, 300A C-18, 5um, 100A, Hydrophillic endcap C-18, 5um, 100A Fused Silica Column Bodies and transfer tubing Deactivated Fused Silica Tubing, sold in 1 meter length SP-009 SP-010 SP um ID x 363 um OD Deactivated methyl 100um ID x 365um OD Deactivated methyl 250 um ID x 350um OD, Deactivated Methyl Un-Deactivated Fused Silica, sold in 1 meter length SP-059 SP-060 SP-061 SP-062 SP-063 SP-064 SP-065 SP-066 5um ID x 363um OD, Un-Deactivated 10um ID x 363um OD, Un-Deactivated 20um ID x 363um OD, Un-Deactivated 50um ID x 363um OD, Un-Deactivated 75um ID x 363umOD, Un-Deactivated 100um ID x 363um OD, Un-Deactivated 200um ID x x363um OD, Un-Deactivated 250um ID x 363 um OD, Un-Deactivated Fused tubing Cutter SP-019 Precision Tube Cutter for fused silica. 19.

20 Nanobaume Operating Instructions REVISION Western Fluids Engineering LLC Lake Elsinore CA