Waters Positive Pressure-96 Processor

Transcription

1 CONTENTS I. SYSTEM OVERVIEW II. a. Features INSTALLING THE WATERS POSITIVE a. Installation overview b. Site requirements c. Unpacking the processor d. Installing the processor I. SYSTEM OVERVIEW a. Features The offers state-of-the-art operation for solid-phase extraction (SPE), filtration in plates and 1cc flangless cartridges in 96-well formats. Each of the 96 holes in the processor is restricted in order to maintain constant pressure, even if all the plate well positions are not filled. Positive pressure processing offers many advantages over traditional vacuum manifold processing. III. USING THE WATERS POSITIVE a. Configuring the trays b. Plate compression c. Operating the manifold d. Controlling positive pressure with flow restrictors e. Adjusting the extraction flow IV. MAINTAINING THE WATERS POSITIVE a. Maintenance considerations b. Replacing the 96-place sealing gasket c. Cleaning the 96-place sealing gasket d. Storage requirements for the 96-place sealing gasket e. Cleaning the device s exterior V. APPENDICES a. Safety Advisories i. Warning symbols ii. Task-specific hazard warnings iii. Burst Warning iv. Caution symbol v. Warnings that apply to all Waters instruments vi. Handling symbols b. Spare Parts c. Safety considerations i. Safety advisories d. Operating this device i. Applicable symbols ii. Audience and purpose iii. Intended use of the Waters Positive Pressure-96 Processor Positive Pressure-96 Processor. The advantages of positive pressure processing include: Reproducible analyte recoveries the manifold gasket with 96 independent ports provides restricted output of inert gas at every port. The processor exerts the same motive force to every port whether it has a full well, dry well or no well access at all. This provides the most uniform SPE extraction which may improve the reproducibility of analyte recoveries. Improved flow for viscous samples the processor allows for gas pressures of 60 to 100 psi (optimal gas pressure is 80 psi), supplying greater motive force for viscous sample flow. Adjusting the pressure up or down provides smooth, immediate flow response and high flow control on variable viscosity plasma samples in a 96-well plate. Fits all 96-well plate formats the processor is self-adjusting to plate height, thus accommodating most 96-well plate formats.

2 Easy-to-use design simply assemble the loaded SPE and collection plates, and position the assembly on the processor slide tray platform. Slide the tray to the rear of the processor until the assembly stops under the manifold. Two toggle switches on the sides of the unit activate the compression mechanism. If the user lets go of the buttons too soon, the manifold mechanism will automatically open. This unique built-in safety feature is designed to keep the user hands clear of moving parts during the compression and decompression cycle, ensuring the safety of the operator. c. Unpacking the processor The processor is shipped with the following items: Operator s guide Installation kit μelution positive pressure spacer 10 ml x 24 well waste bin tray 1 ml x 96 well collection tray Quick Start Guide Superior SPE flow control the processor exerts fine control on the SPE flow through the plate. The inert gas flow can be adjusted by switching the SPE Flow Rate selector to the left (Low Flow setting). The gas delivered to the Positive Pressure-96 Processor through the adjustable flow regulator and through the rotometer (on the left). The rotometer is controlled by the needle valve located at its base. This will provide a uniform and controlled flow through the entire SPE 96-well plate without the impact on flow from empty wells. Handling viscous samples the gas delivery system can provide rapid gas flow to the manifold. This flow is controlled by adjusting the regulator located below the pressure gauge labeled Max Flow. The pressure range is 0 to 100 psi and is used to maximize flow through the SPE 96-well plate for processing viscous samples, and significantly reducing the risk of well plugging by such samples. II. INSTALLING THE WATERS POSITIVE a. Installation overview The is for laboratory use only. It must be set up inside a hood vent whenever possible and requires an inert gas supply of 60 to 100 psig. d. Installing the processor Compressed nitrogen is the recommended pressure source in order to ensure inert atmosphere operation. The is supplied with an installation kit which includes: 6 feet of 1/8-inch i.d. (1/4-inch o.d.) polyethylene tubing Connectors to 1/8-inch NPT or 1/4-inch NPT Use the tubing and fittings provided to connect the unit to a regulated oil free gas source. b. Site requirements Indoor-use only Maximum altitude: 2000 meters Ambient temperature range: 5 to 40 C Maximum relative humidity of 80% for temperatures up to 31 C, decreasing linearly to 50% relative humidity at 40 C Installation Category II Pollution Degree 2 Environment Regulated source pressure. 2

3 Tip: The optimum source pressure is 80 psig, the minimum pressure is 60 psig, and the maximum pressure is 100 psig. Caution: The does not include a filter on the source gas input. A clean, oil-free gas source is essential to prevent sample contamination. III. USING THE WATERS POSITIVE a. Configuring the plates The provides the means to batch process solid-phase extraction in all Waters 96-well plates, filtration plates and 1 cc flangeless cartridges in the 96-position format. The processor accommodates both integrated and modular plates stacked onto a variety of collection plates. The holder maintains plate guides for the proper positioning of the collection plate throughout the sample sequence. Both the holder and collection plate should be stacked to match precisely so that when they are correctly aligned, the assembly sits level on the slide tray platform. The Waters 96-well plate formats align to the collection plates while the Waters μelution plates are placed onto the μelution positive pressure spacer, and then stacked onto the collection plate so that sample position for both of these integrated plates are preserved throughout the sample sequence. platform. Each column will be pressurized equally, even if wells or cartridges are omitted. Refer to Operating the manifold on page 4 for further explanation. Slide the slide tray to the rear of the processor until it reaches the stop locating it under the manifold. The two toggle switches used to activate the compression mechanism are located on opposite sides of the processor. Both toggle switches must be pressed at the exact same time to open or close the compression mechanism. Activate the plate compression mechanism by depressing the switches until the manifold has stopped moving (2 to 3 seconds). If you let go too soon, the manifold mechanism will automatically open. This is a safety feature designed to keep your hands clear of moving parts during the compression and decompression cycle. Sealing the plate. Decompression of the rack assembly is activated similarly. Both toggle switches must be depressed simultaneously until the manifold has stopped moving. Slide the rack assembly forward until it reaches the stop and proceed with the next step of the method. The compression speed is pre-set and is not adjustable. The pressure applied to the compression system is pre-set and is not adjustable. Installing the plate in the. b. Plate compression Assemble the appropriate 96-well plate, μelution plate, or 1 cc flangeless cartridges and collection plate and position the assembly on the slide tray c. Operating the manifold The 96 holes in the processor manifold are restricted in order to maintain the manifold pressure even if all of the positions are not filled by columns. With no cartridges in place or if some of the wells in the plate are empty, there is approximately 0.75 ±.05 scfh of flow through a hole at 25 psig. 3

4 d. Controlling positive pressure with flow restrictors Inhibiting the flow with the constrictors is helpful in two ways: The amount of gas used is minimized as compared to a system with no inhibitors. The pressure remains high in the wells or cartridges containing liquid as compared to the empty or dry wells or cartridges. e. Adjusting the extraction flow The has a two-level gas delivery system used to pressurize the SPE plate. One part of the system is controlled by the rotometer located on the left side of the instrument panel. By switching the SPE Flow Rate selector to the left, Adjust Flow setting, the gas is delivered to the Manifold through the adjustable flow regulator and then through the rotometer controlled by the needle valve located at its base. The rotometer provides a very precise and slow flow through the plate wells or the cartridges by limiting the gas flow to the manifold from 0 to 2.5 SCFH. turning the SPE Flow Rate selector to off, no gas will flow to the manifold. It should be in the off position during the compression and decompression cycles and when not in use. IV. MAINTAINING THE WATERS POSITIVE a. Maintenance considerations Safety and handling Observe these warning and caution advisories when you perform maintenance operations on your system. Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the. Consult the Material Safety Data Sheets regarding the solvents you use. Caution: The device is constructed of anodized aluminum, stainless steel, and plastics. To prevent damage to the device, clean solvent spills and overflow immediately. Key Features. A. Manifold Head B. Toggle Switches Raise and lower manifold head (1 on each side) C. Rotometer Sets the gas flow for the low flow setting D. Low Flow Regulator E. Max Flow Regulator F. Selector Valve Used to select LOW or MAX gas flow through the device. Tip: The rotometer needle valve is for adjustment only and must never be used as an on off valve. Use the selector valve to turn off the gas flow. The second part of the gas delivery system provides for rapid gas flow to the manifold. This flow is controlled by adjusting the regulator located below the pressure gauge labeled Max Flow. The pressure range is 0 to 80 psig and is used to maximize flow through the plate wells or the cartridges for processing highly viscous samples or to dry the well or cartridge in situ. By switching the SPE Flow Rate selector to Max Flow, gas is introduced to the manifold at the pressure set on the Max Flow regulator as indicated by the gauge located directly above it. By b. Replacing the 96-place sealing gasket Required materials 96-place sealing gasket 3/16-inch hex key To replace the 96-place sealing gasket: 1. Using the enclosed hex key, remove the two 1/4-20 screws that fasten the manifold to the unit. 2. Lift the manifold off the standoffs and place it upside down on the table. Rationale: Removing the manifold helps you accurately align the replacement 96-place sealing gasket. 3. Remove the old 96-place sealing gasket from the manifold. 4. Attach the enclosed screws to the threaded holes on each corner on the bottom of the manifold. 5. Remove the backing of the replacement 96-place sealing gasket. Use the screws for aligning and adhere the replacement 96-place sealing gasket. 6. Once the sealing gasket is attached, remove the four screws. 7. Carefully set the manifold on the standoffs. Fasten the manifold with the screws. 8. Using the 3/16 hex key, tighten the screws. 4

5 c. Cleaning the 96-place sealing gasket Use a soft cloth, dampened with methanol, to clean the silicone rubber sealing gasket. d. Storage requirements for the 96-place sealing gasket Store the 96-place sealing gasket at 4 C. The gasket has a 6 month shelf life from the shipping date. e. Cleaning the device s exterior Use a soft cloth, dampened with water, to clean the outside of the. To clean the surfaces of the device, you should: Always use eye and hand protection during the cleaning process. Apply the water to a clean cloth only, and then wipe the device down. Never spray or apply the water directly to any surface of the device. V. APPENDICES A. Safety Advisories Waters instruments display hazard symbols designed to alert you to the hidden dangers of operating and maintaining the instruments. Their corresponding user guides also include the hazard symbols, with accompanying text statements describing the hazards and telling you how to avoid them. This appendix presents all the safety symbols and statements that apply to the entire line of Waters products. Contents: Warning symbols Caution symbol Warnings that apply to all Waters instruments i. Warning symbols Warning symbols alert you to the risk of death, injury, or seriously adverse physiological reactions associated with an instrument s use or misuse. Heed all warnings when you install, repair, and operate Waters instruments. Waters assumes no liability for the failure of those who install, repair, or operate its instruments to comply with any safety precaution. ii. Task-specific hazard warnings The following warning symbols alert you to risks that can arise when you operate or maintain an instrument or instrument component. Such risks include burn injuries, electric shocks, ultraviolet radiation exposures, and others. If the following symbols appear in a manual s narratives or procedures, their accompanying text identifies the specific risk and explains how to avoid it. iii. Burst warning Warning: General risk of danger. When this symbol appears on an instrument, consult the instrument s user documentation for important safety-related information before you use the instrument. This warning applies to Waters instruments fitted with nonmetallic tubing. Warning: Pressurized nonmetallic, or polymer, tubing can burst. Observe these precautions when working around such tubing: Wear eye protection. Extinguish all nearby flames. Do not use tubing that is, or has been, stressed or kinked. Do not expose nonmetallic tubing to incompatible compounds like tetrahydrofuran (THF) and nitric or sulfuric acids. Be aware that some compounds, like methylene chloride and dimethyl sulfoxide, can cause nonmetallic tubing to swell, which significantly reduces the pressure at which the tubing can rupture. iv. Caution symbol The caution symbol signifies that an instrument s use or misuse can damage the instrument or compromise a sample s integrity. The following symbol and its associated statement are typical of the kind that alert you to the risk of damaging the instrument or sample. Caution: To avoid damage, do not use abrasives or solvents to clean the instrument s case. 5

6 v. Warnings that apply to all Waters instruments When operating this device, follow standard quality control procedures Attention: and the equipment Changes guidelines or modifications in this to section. this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment. Important: Toute modification sur cette unité n ayant pas été expressément approuvée par l autorité responsable de la conformité à la réglementation peut annuler le droit de l utilisateur à exploiter l équipement. Achtung: Jedwede Änderungen oder Modifikationen an dem Gerät ohne die ausdrückliche Genehmigung der für die ordnungsgemäße Funktionstüchtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems führen. Avvertenza: qualsiasi modifica o alterazione apportata a questa unità e non espressamente autorizzata dai responsabili per la conformità fa decadere il diritto all utilizzo dell apparecchiatura da parte dell utente. Atencion: cualquier cambio o modificación efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del cumplimiento puede anular la autorización del usuario para utilizar el equipo. 注意 : 未經有關法規認證部門允許對本設備進行的改變或修改, 可能會使使用者喪失操作該設 備的權利 注意 : 未经有关法规认证部门明确允许对本设备进行的改变或改装, 可能会使使用者丧失操 作该设备的合法性 주의 : 규정준수를책임지는당사자의명백한승인없이이장치를개조또는변경할경우, 이장치를운용할수있는사용자권한의효력을상실할수있습니다. 注意 : 規制機関から明確な承認を受けずに本装置の変更や改造を行うと 本装置のユー Warning: Use caution when working with any polymer tubing under pressure: Always wear eye protection when near pressurized polymer tubing. Extinguish all nearby flames. Do not use tubing that has been severely stressed or kinked. Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated nitric or sulfuric acids. Be aware that methylene chloride and dimethyl sulfoxide cause nonmetallic tubing to swell, which greatly reduces the rupture pressure of the tubing. Attention: Manipulez les tubes en polymère sous pression avec precaution: Portez systématiquement des lunettes de protection lorsque vous vous trouvez à proximité de tubes en polymère pressurisés. Eteignez toute flamme se trouvant à proximité de l instrument. Evitez d utiliser des tubes sévèrement déformés ou endommagés. Evitez d utiliser des tubes non métalliques avec du tétrahydrofurane (THF) ou de l acide sulfurique ou nitrique concentré. Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le gonflement des tuyaux non métalliques, ce qui réduit considérablement leur pression de rupture. Vorsicht: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere Vorsicht angebracht: In der Nähe von unter Druck stehenden Polymerschläuchen stets Schutzbrille tragen. Alle offenen Flammen in der Nähe löschen. Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind. Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder konzentrierte Salpeter- oder Schwefelsäure verwenden. Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische Schläuche quellen; dadurch wird der Berstdruck des Schlauches erheblich reduziert. ザーとしての承認が無効になる可能性があります 6

7 Attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione: Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. Spegnere tutte le fiamme vive nell ambiente circostante. Non utilizzare tubi eccessivamente logorati o piegati. Non utilizzare tubi non metallici con tetraidrofurano (THF) o acido solforico o nitrico concentrati. Tenere presente che il cloruro di metilene e il dimetilsolfossido provocano rigonfiamenti nei tubi non metallici, riducendo notevolmente la pressione di rottura dei tubi stessi. Advertencia: se recomienda precaución cuando se trabaje con tubos de polímero sometidos a presión: El usuario deberá protegerse siempre los ojos cuando trabaje cerca de tubos de polímero sometidos a presión. Si hubiera alguna llama las proximidades. No se debe trabajar con tubos que se hayan doblado o sometido a altas presiones. Es necesario utilizar tubos de metal cuando se trabaje con tetrahidrofurano (THF) o ácidos nítrico o sulfúrico concentrados. Hay que tener en cuenta que el cloruro de metileno y el sulfóxido de dimetilo dilatan los tubos no metálicos, lo que reduce la presión de ruptura de los tubos. 警告 : 當在有壓力的情況下使用聚合物管線時, 小心注 意以下幾點 當接近有壓力的聚合物管線時一定要戴防護眼鏡 熄滅附近所有的火焰 不要使用已經被壓癟或嚴重彎曲管線 不要在非金屬管線中使用四氫呋喃或濃硝酸或濃硫酸 要了解使用二氯甲烷及二甲基亞楓會導致非金屬管 線膨脹, 大大降低管線的耐壓能力 警告 : 当有压力的情况下使用管线时, 小心注意以下几点 : 当接近有压力的聚合物管线时一定要戴防护眼镜 熄灭附近所有的火焰 不要使用已经被压瘪或严重弯曲的管线 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸 要了解使用二氯甲烷及二甲基亚枫会导致非金属管 线膨胀, 大大降低管线的耐压能力 경고 : 가압폴리머튜브로작업할경우에는 주의하십시오. 가압폴리머튜브근처에서는항상보호안경을 착용하십시오. 근처의화기를모두끄십시오. 심하게변형되거나꼬인튜브는사용하지마십시오. 비금속 (Nonmetallic) 튜브를테트라히드로푸란 (Tetrahydrofuran: THF) 또는농축질산또는황산과 함께사용하지마십시오. 염화메틸렌 (Methylene chloride) 및디메틸술폭시드 (Dimethyl sulfoxide) 는비금속튜브를부풀려 튜브의파열압력을크게감소시킬수있으므로 유의하십시오. 警告 : 圧力のかかったポリマーチューブを扱うときは 注意してください 加圧されたポリマーチューブの付近では 必ず保護メ ガネを着用してください 近くにある火を消してください 著しく変形した または折れ曲がったチューブは使用 しないでください 非金属チューブには テトラヒドロフラン (THF) や高 濃度の硝酸または硫酸などを流さないでください 塩化メチレンやジメチルスルホキシドは 非金属チュ ーブの膨張を引き起こす場合があり その場合 チュ ーブは極めて低い圧力で破裂します Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Attention: L utilisateur doit être informé que si le matériel est utilisé d une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d être défectueuses. Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass bei unsachgemäßer Verwenddung des Gerätes die eingebauten Sicherheitseinrichtungen unter Umständen nicht ordnungsgemäß funktionieren. 7

8 Attenzione: si rende noto all utente che l eventuale utilizzo dell apparecchiatura secondo modalità non previste dal produttore può compromettere la protezione offerta dall apparecchiatura. Advertencia: el usuario deberá saber que si el equipo se utiliza de forma distinta a la especificada por el fabricante, las medidas de protección del equipo podrían ser insuficientes. 警告 : 使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用, 那麼該設備所提供的保護將被消弱 警告 : 使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用, 那么该设备所提供的保护将被削弱 경고 : 제조업체가명시하지않은방식으로장비를사용할경우장비가제공하는보호수단이제대로작동하지않을수있다는점을사용자에게반드시인식시켜야합니다. 警告 : ユーザーは 製造元により指定されていない方法で機器を使用すると 機器が提供している保証が無効になる可能性があることに注意して下さい vi. Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments. B. Spare Parts Spare part information is listed in the table below. Spare parts Item Waters Positive Pressure-96 Processor 96-well plate, polypropylene 96-well 1 ml collection plate Polypropylene cap mat for 1 ml plate 96-flangeless cartridges holder 96-place sealing gasket (special storage requirement: at 4 C and with shelf life of 6 months) µelution positive pressure spacer Gas supply adapter, includes 1/8-inch to 1/4-inch NPT fitting, 6 feet of 1/4-inch tubing 10 ml 24 waste collection plate Volume (ml) Quantity/ package Part number ml ml cc Keep upright! Keep dry! Fragile! Use no hooks! 8

9 C. Safety considerations Some reagents and samples used with Waters instruments and devices can pose chemical, biological, and radiological hazards. You must know the potentially hazardous effects of all substances you work with. Always follow Good laboratory Practice and consult your organization s safety representative for guidance. i. Safety advisories Consult Appendix A for a comprehensive list of warning and caution advisories. D. Operating this device When operating this device, follow standard quality-control (QC) procedures and the guidelines presented in this section. i. Applicable symbols Symbol Definition Manufacturer Consult instructions for use ii. Audience and purpose This guide is intended for personnel who install, operate, and maintain the. iii. Intended use of the The is for research use only and is not intended for use in diagnostic applications. Waters, and The Science of What s Possible are registered trademarks of Waters Corporation. All other trademarks are the property of their respective owners Waters Corporation. Produced in the U.S.A. February EN KP-PDF Waters Corporation 34 Maple Street Milford, MA U.S.A. T: F: