Connecting a SSL/PTV Backflush System

Transcription

1 This section provides instructions for connecting your SSLBKF/PTVBKF injector modules with pre-column and analytical capillary column into the oven. Refer to the chapters SSLBKF Injector Module and PTVBKF Injector Module in the TRACE 1300/TRACE 1310 User Guide. The operation consists of the following operating procedures: To assemble the Tee connector on the mounting bracket on page 319 To install the mounting bracket into the GC oven on page 321 To connect backflush line, precolumn, and capillary column on page 322 To install of a second backflush system on page 325 CAUTION Before starting make sure the SSLBKF/PTVBKF injector module is correctly installed into its seat. To assemble the Tee connector on the mounting bracket Retaining Nut Graphite/Vespel Ferrule Backflush Line Fittings Graphite/Vespel Ferrule Retaining Nut Tee Connector Clamp Clip Nut Threaded Shaft Mounting Bracket Top Nut Tee Connector Assembly Bottom 1. Remove the nut and the top end of the mounting bracket. This nut secures the two sections of the mounting bracket. See Figure 1. Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 319

2 Figure 1. Assembling Tee Connector and Mounting Bracket (1) Nut Top End Note Wait reinstalling the top end of the mounting bracket and the nut. These parts will be reinstalled during the installation of the mounting bracket into the GC oven. See To install the mounting bracket into the GC oven on page Slide the clip and the clamp on the body of the mounting bracket. See Figure 2. Figure 2. Assembling Tee Connector and Mounting Bracket (2) Clamp Clip 3. Screw the part of threaded shaft of the Tee connector with the smaller diameter into the Tee connector, then screw the nut on the threaded shaft. Use a 2.5 mm key to tighten the shaft. See Figure 3. Figure 3. Assembling Tee Connector and Mounting Bracket (3) Threaded Shaft Threaded Shaft Tee Connector Nut 320 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

3 4. Screw the Tee connector into clamp. Turn the threaded shaft until it protrudes the clamp, then turn the nut until it grip the clamp. See Figure 4. Figure 4. Assembling Tee Connector and Mounting Bracket (4) 5. Move the Tee connector support on the lower part of the mounting bracket, then finger-tighten the nut up to grip the clamp without overtightening. This allow to adjust the position of the Tee connector during the installation of backflush line, pre-column, and capillary column. 6. At this point mount the mounting bracket into the oven of the GC. See To install the mounting bracket into the GC oven on page 321. To install the mounting bracket into the GC oven Install the mounting bracket near the front of the GC oven on the right-hand side. This will keep the mounting bracket out of the way of the column. Proceed as follows: 1. Make sure that the nut at the top end of the mounting bracket are removed. See Figure 1 on page Line up the bottom of the mounting bracket into two holes on the bottom side of the GC oven. 3. Hold the mounting bracket directly upright, and twist the top end of it so that it is securely attached to the GC oven holes. 4. Let out the top of the mounting bracket until it is long enough to be secured into two holes on the bottom of the GC oven. 5. Replace the nut. 6. Tighten the nut until the mounting bracket is securely attached to the top and bottom of the GC oven. Figure 5 shows the result of the operation. Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 321

4 Figure 5. Mounting Bracket Installed in the GC Tee Connector Clamp 7. At this point connect backflush line, precolumn, and capillary column to the Tee connector. See To connect backflush line, precolumn, and capillary column on page 322. To connect backflush line, precolumn, and capillary column The result of this operation is schematically shown in Figure 6. Figure 6. Connecting Backflush Line, Precolumn, and Capillary Column Precolumn Backflush Line Capillary Column 322 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

5 Note Could be necessary to adjust the position of the Tee connector when installing backflush line, precolumn, and column to stress as minimal as possible. 1. Connect the backflush line metal tube coming from the bottom of the SSLBKF injector module or PTVBKF injector module to the Tee connector. Tip The backflush line must be bent as required by the position of the Tee connector on the mounting bracket. a. Slide the retaining nut and the ferrule onto the end of the backflush line. b. Insert the backflush line into the Tee connector. c. Finger-tighten the retaining nut until it starts to grip the backflush line. d. Use the 5 mm wrench to tighten the retaining nut. Use enough pressure necessary to obtain a good seal without overtighten. 2. Connect the pre-column to the Tee connector. Note Always use original Thermo Fisher Scientific precolumns. The use of pre-columns not meeting the specifications of our products does not ensure a good operation of the instrument and may even compromise the analytical results. Thermo Fisher Scientific pre-column (2 m; 10 m) are mounted on the appropriate rack and identified with a relevant label fixed on the rack. a. Place the pre-column on the column support. b. Slide the retaining nut and the 0.8 mm Vespel/graphite ferrule onto the precolumn with the flat end facing towards the Tee connector. Be careful to avoid damaging the graphite ferrule when inserting the pre-column. Always use a new ferrule of the correct diameter. c. Cut 1 cm from the precolumn end. d. Insert the pre-column into the Tee connector in the side with the dot that it must be normally be turned towards the ceiling of the GC oven. e. Finger-tighten the pre-column retaining nut until it starts to grip the pre-column. f. Use the 1/4-in. wrench to tighten the retaining nut. Use enough pressure necessary to obtain a good seal without overtighten. 3. Connect the precolumn to the injector base. a. Slide the retaining nut and the graphite ferrule onto the fused silica precolumn (0.53 mm ID; 2 m length) with the bevelled end facing towards the injector. b. Cut 1 cm from the precolumn. c. Insert the precolumn into the injector and slide the ferrule up to the injector base.insert the pre-column about 3 cm into the bottom of the injector. Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 323

6 d. Finger-tighten the pre-column retaining nut until it starts to grip the precolumn. e. Use the 6 mm wrench to tighten the retaining nut. Use enough pressure necessary to obtain a good seal without overtighten. 4. Connect the capillary column to the Tee connector and to the pre-column. See Figure 7. Figure 7. Precolumn and Capillary Column Connection Precolumn Backflush Line 35 mm Capillary Column a. Place the column on the column support. b. Slide the proper retaining nut and Vespel/graphite ferrule onto the column with the flat end facing towards the Tee connector. Be careful to avoid damaging the ferrule when inserting the column. c. Cut 1 cm from the column end. d. Use typewriter correction fluid or a felt-tipped pen to mark the position of the ferrule bottom 35 mm from the end of the column. e. Carefully insert the column into the precolumn up to reach the marked position. The capillary column end must protrudes the Tee connector; if not, repeat the operations. f. Finger-tighten the column retaining nut until it starts to grip the column. g. Use the 1/4-in. wrench to tighten the retaining nut. Use enough pressure necessary to obtain a good seal. 324 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

7 5. Connect the analytical column to the detector base. a. Slide the retaining nut and the graphite ferrule onto the capillary column with the bevelled end facing the detector base. Be careful to avoid damaging the graphite ferrule when inserting the column. b. Cut 2 3 cm from the column end. c. Insert the column into the detector base body and slide the ferrule up to the detector base. d. Finger-tighten the column retaining nut until it starts to grip the column. e. Push the column through the detector base according to the detector in use. f. Use the 6 mm wrench to tighten the retaining nut. Use enough pressure necessary to obtain a good seal without overtighten. To install of a second backflush system If a second SSL/PTV Backflush system is installed, you can connect both the systems by using two Tee connectors mounted on the same mounting bracket as schematically shown in Figure 8. Figure 8. Schematically Connection of a Second Backflush System Precolumn Backflush Line Capillary Column Precolumn Backflush Line Capillary Column Tip Turn the Tee connector as required to allow the installation of the two pre-columns. Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 325

8 326 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific