Status and recovery of indigenous crayfish populations after recent crayfish plague outbreaks in the Czech Republic

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1 Status and recovery of indigenous crayfish populations after recent crayfish plague outbreaks in the Czech Republic E. Kozubíková-Balcarová,. Beran, Z. Ďuriš, D. Fischer, I. Horká, J. Svobodová, A. Petrusek Supplementary file 1. Maps of catchments of the watercourses in which the described crayfish mass mortalities occurred. Relevant watercourses and their tributaries are highlighted. Geographic names mentioned in the descriptions of the outbreaks are indicated by abbreviations (water courses and bodies in blue, settlements in black). Potential migration barriers are indicated in the maps, and sites where crayfish affected by mass mortalities were found are highlighted. In case the mass mortality is marked by a single symbol, it is placed at the exact site where crayfish were found; if the extent of mass mortalities is marked by multiple symbol, these are spread over the area of the outbreak (which affected also the regions between such symbols). The same distinction between single and multiple symbols should be made if sites of crayfish presence are indicated on the map. Supplementary Figure 1. Catchment of the Pšovka brook (crayfish mass mortalities ) Supplementary Figure 2. Catchment of the River Olše (crayfish plague outbreak 2006) including the Křivec brook (2004) Supplementary Figure 3. Catchment of the Bojovský brook (crayfish plague outbreak 2005) Supplementary Figure 4. Catchment of the Úpořský brook (crayfish plague outbreak 2005) Supplementary Figure 5. Catchment of the tributary of Pěnenský pond (crayfish plague outbreak 2007) Supplementary Figure 6. Catchment of the Žebrákovský brook (crayfish plague outbreak 2008) Supplementary Figure 7. Catchment of the River Senice (crayfish plague outbreak 2008) Supplementary Figure 8. Catchment of the brook Besének (crayfish plague outbreak 2009) Supplementary Figure 9. Catchment of the Zákolanský brook (crayfish plague outbreak 2009) Supplementary Figure 10. Catchment of the River itavka (crayfish plague outbreak 2011) Supplementary Figure 11. Catchment of the Černý brook (crayfish plague outbreak 2014)

2 Supplementary Figure 1. Catchment of the Pšovka brook Ps Pšovka brook Ha pond Harasov Sta pond Štampach Str pond Stříbrník KD village Kokořínský Důl Kok village Kokořín Kon village Konrádov Vo village Vojtěchov Ps Vo Ps Kon Str KD Kok Ha Sta Ps 5 km extent of Astacus astacus and Astacus leptodactylus mass mortalities in extent of known A. astacus survival in 1999 site of A. astacus transfer from the upstream refuge in 2005 present contact zone of A. astacus and Orconectes limosus

3 Supplementary Figure 2. Catchment of the River Olše including the Křivec brook Tr O K O Hl By Hl O River Olše K Křivec brook Hl Hluchová brook Ja Jasení Kop O Kos Hl Kop Kopytná Kos Kostkov brook o omná brook Os Ošetnický brook By village Bystřice Jab village Jablunkov Pi village Písek Kop o Ja o Ja Kos Jab o O Os Pi O Tr town Třinec Os 5 km potential barriers site of Astacus astacus mass mortality in 2004 (Křivec brook) extent of A. astacus mass mortality in 2006 (River Olše)

4 Supplementary Figure 3. Catchment of the Bojovský brook Bo Vr Bo Bj Bo Ci Bo Bo Bojovský brook Vr reservoir Vrané on the River Vltava Bj village Bojov Ci village Čisovice site of observed Astacus astacus mass mortality in 2005 area with known occurrence of Orconectes limosus in 2011

5 Supplementary Figure 4. Catchment of the Úpořský brook Be Be U Ho U U M Ku Ho M U Úpořský brook Be River Berounka Ho Hořejší brook M brook Míza Ku Kučerův pond migration barrier extent of Austropotamobius torrentium mass mortality in 2005 area of known A. torrentium survival in 2005

6 Supplementary Figure 5. Catchment of the tributary of Pěnenský pond HP Pe Pp Pe Si HP village Horní Pěna Pe tributary of the Pěnenský pond Pp Pěnenský pond Si pond Sígl migration barrier extent of Astacus astacus mass mortality in 2007 area of A. astacus survival after the crayfish plague outbreak

7 Supplementary Figure 6. Catchment of the Žebrákovský brook Kr Ze p Ze Žebrákovský brook Kr reservoir Kristiánka p ánský pond Sa River Sázava migration barrier extent of Astacus astacus mass mortality in 2008 area of A. astacus survival in 2008 Sa Ze Ze Sa

8 Supplementary Figure 7. Catchment of the River Senice S S VS i S F H S 5 km S River Senice F village Francova hota H village Horní ideč i village idečko VS village Valašská Senice site of Astacus astacus mass mortality in 2008

9 Supplementary Figure 8. Catchment of the brook Besének Be Sv Be Be Sv Ti Be brook Besének Sv River Svratka Ti town Tišnov site of Astacus astacus mass mortality in 2009

10 Supplementary Figure 9. Catchment of the Zákolanský brook Za Dr Dr Dr Po Za NM Ci Op Za Za Zákolanský brook Dr Dřetovický brook Op Okořský pond Po Podholí NM Nový Mlýn Ci village Číčovice migration barriers site of Austropotamobius torrentium mass mortality in 2009

11 Supplementary Figure 10. Catchment of the River itavka Bz Ku St St Ce St Ce Ce Su Su site of Astacus astacus mass mortality in 2011 known occurrence of Orconectes limosus in 2013 Eriocheir sinensis observations in 2012 and 2013 fj St Ce Ja Ce Oh Oh River itavka Bz Bzovský brook Ce Červený brook Ja Ce Ob Ob Ja Jalový brook Ku Kublovský brook Ob Obecnický brook Oh Ohrazenický brook St Stroupinský brook Pr Su Suchomastský brook Vp Vysokopecký pond Vp Pr town Příbram fj farmstead Janov

12 Supplementary Figure 11. Catchment of the Černý brook C C UM C C PC C Černý brook UM pond U Mlýnečku PC village Pec pod Čerchovem extent of Astacus astacus mass mortality in 2014