North Carolina State University

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1 TOLERANCE OF CARPETGRASS AND ANNUAL BLUE-EYED GRASS CONTROL WITH FLAZASULFURON 25DG APPLIED FROM DORMANCY TO FULL GREENUP Trial ID: 06-T26 Protocol ID: 06-T26 Location: HIDDEN VALLEY GC Study Director: L.S. WARREN Investigator: Fred Yelverton General Trial Information Study Director: L.S. WARREN Title: PROFESSOR Affiliation: NORTH CAROLINA STATE UNIVERSITY Postal Code: Investigator: Fred Yelverton Title: PROFESSOR Affiliation: NORTH CAROLINA STATE UNIVERSITY Postal Code: Trial Location City: WILLOW SPRINGS Trial Status: COMPLETED State/Prov.: NC Postal Code: Initiation Date: Country: USA Objectives: TO EVALUATE TOLERANCE OF CARPETGRASS AND ANNUAL BLUE-EYED GRASS CONTROL WITH FLAZASULFURON 25DG APPLIED FROM DORMANCY THROUGH FULL GREENUP Cooperator/Landowner Cooperator: MICHAEL GREGORY Country: USA Organization: HIDDEN VALLEY GOLF COURSE Phone No: 919/ Address 1: 7900 HIGHWAY 55 S City: WILLOW SPRINGS State/Prov: NC Postal Code: Crop Description Crop 1: AXOAF Axonopus affinis BBCH Scale: BGWE Narrow-leaf carpetgrass Pest Description Pest 1 Type: W Code: SISBE Sisyrinchium bermudiana Common Name: Blue-eyed grass, narrowleaf Pest 2 Type: W Code: POAAN Poa annua Common Name: Annual bluegrass Site and Design Plot Width, Unit: 4 FT Site Type: TURF - GOLF COURSE ROUGH Plot Length, Unit: 5 FT Tillage Type: NA Replications: 4 Study Design: Randomized Complete Block Soil Description % OM: 1.19 Texture: CLAY LOAM ph: 5.4 CEC: 5.6 Fert. Level: GOOD Moisture Conditions Overall Moisture Conditions: MAR 0.83"; APR 3.30"; MAY 2.14" Closest Weather Station: HARNETT COUNTY AIRPORT Distance: 10 Unit: MI

2 Application Description A B C Application Date: Time of Day: 2:00 PM 10:00 AM 4:15 PM Application Method: SPRAY SPRAY SPRAY Application Timing: DORMANT 60% GREEN 100%GREEN Application Placement: BROFOL BROFOL BROFOL Applied By: L.S. WARREN L.S. WARREN L.S. WARREN Air Temperature, Unit: 65 F 60 F 72 F % Relative Humidity: Wind Velocity, Unit: 4.0 MPH 0.8 MPH 1.2 MPH Dew Presence (Y/N): N Y N Soil Temperature, Unit: 57 F 56 F 73 F Soil Moisture: WET MOIST WET % Cloud Cover: Crop Stage At Each Application A B C Crop 1 Code, BBCH Scale: AXOAF BGWE AXOAF BGWE AXOAF BGWE Stage Scale Used: BBCH BBCH BBCH Stage Majority, Percent: DORMANT 95 GREEN 60 GREEN 100 Height, Unit: 0.75 IN 1.5 IN 2.0 IN Pest Stage At Each Application A B C Pest 1 Code, Disc., Scale: SISBE W SISBE W SISBE W Stage Majority, Percent: VEG 100 VEG 100 FLOWER 100 Diameter, Unit: 4 IN 4 IN 5 IN Height, Unit: 1.5 IN 1.5 IN 2.5 IN Density, Unit: 1.5 FT2 1.5 FT2 1.5 FT2 Pest 2 Code, Disc., Scale: POAAN W POAAN W POAAN W Stage Majority, Percent: TILLER 100 SEEDED 100 SEEDED 100 Height, Unit: 1.25 IN 1.5 IN 2.5 IN Density, Unit: 1 FT2 1 FT2 1 FT2 Application Equipment A B C Appl. Equipment: BACPSR BACPSR BACPSR Operating Pressure, Unit: 28 PSI 28 PSI 28 PSI Nozzle Type: FLAT FAN FLAT FAN FLAT FAN Nozzle Size: XR 8002VS XR 8002VS XR 8002VS Nozzle Spacing, Unit: 10 IN 10 IN 10 IN Band Width, Unit: 40 IN 40 IN 40 IN Boom Length, Unit: 40 IN 40 IN 40 IN Boom Height, Unit: 10 IN 10 IN 10 IN Ground Speed, Unit: 3 MPH 3 MPH 3 MPH Carrier: WATER WATER WATER Spray Volume, Unit: 32.5 GPA 32.5 GPA 32.5 GPA Propellant: COMCO2 COMCO2 COMCO2 Tank Mix (Y/N): Y Y Y

3 TOLERANCE OF CARPETGRASS AND ANNUAL BLUE-EYED GRASS CONTROL WITH FLAZASULFURON 25DG APPLIED FROM DORMANCY TO FULL GREENUP Trial ID: 06-T26 Protocol ID: 06-T26 Location: HIDDEN VALLEY GC Study Director: L.S. WARREN Investigator: Fred Yelverton Pest Type Pest Code Crop Code AXOAF AXOAF AXOAF AXOAF AXOAF BBCH Scale BGWE BGWE BGWE BGWE BGWE Crop Name Narrow-leaf> Narrow-leaf> Narrow-leaf> Narrow-leaf> Narrow-leaf> Rating Date Rating Data Type GREENUP GREENUP GREENUP GREENUP GREENUP Days After First/Last Applic Trt-Eval Interval 14 DA-A 21 DA-A 29 DA-A 33 DA-A 7 DA-B No. Name Conc Type Rate Unit Stage Code FLAZASULFURON 25 DG 1.5 oz wt/a DORMANT A 3.5 b 5.5 b 13.0 b 23.5 b 53.8 a 2 FLAZASULFURON 25 DG 3 oz wt/a DORMANT A 1.8 b 3.5 b 3.5 cd 8.8 c 28.8 bc 3 FLAZASULFURON 25 DG 6 oz wt/a DORMANT A 1.5 b 3.3 b 1.8 d 3.8 c 12.5 c 4 CERTAINTY 75 WG 1.25 oz wt/a DORMANT A 3.5 b 6.3 b 11.3 bc 22.5 b 35.0 b 5 FLAZASULFURON 25 DG 1.5 oz wt/a 60%GREEN B 57.5 a 6 FLAZASULFURON 25 DG 3 oz wt/a 60%GREEN B 60.0 a 7 FLAZASULFURON 25 DG 6 oz wt/a 60%GREEN B 55.0 a 8 CERTAINTY 75 WG 1.25 oz wt/a 60%GREEN B 58.8 a 9 FLAZASULFURON 25 DG 1.5 oz wt/a 100% C 10 FLAZASULFURON 25 DG 3 oz wt/a 100% C 11 FLAZASULFURON 25 DG 6 oz wt/a 100% C 12 CERTAINTY 75 WG 1.25 oz wt/a 100% C 13 CHECK 7.5 a 15.0 a 36.3 a 57.5 a 71.3 a LSD (P=.05) Standard Deviation CV Bartlett's X P(Bartlett's X2) 0.033* * 0.002* Replicate F Replicate Prob(F) Treatment F Treatment Prob(F)

4 Pest Type Pest Code Crop Code AXOAF AXOAF AXOAF AXOAF AXOAF BBCH Scale BGWE BGWE BGWE BGWE BGWE Crop Name Narrow-leaf> Narrow-leaf> Narrow-leaf> Narrow-leaf> Narrow-leaf> Rating Date Rating Data Type COVERAGE COVERAGE COVERAGE COVERAGE COVERAGE Days After First/Last Applic Trt-Eval Interval 15 DA-B 22 DA-B 30 DA-B 36 DA-B 7 DA-C No. Name Conc Type Rate Unit Stage Code FLAZASULFURON 25 DG 1.5 oz wt/a DORMANT A 78.8 a 88.8 a 90.0 ab 90.0 a 94.8 a 2 FLAZASULFURON 25 DG 3 oz wt/a DORMANT A 60.0 a 73.8 abc 83.8 ab 78.8 a 82.5 a 3 FLAZASULFURON 25 DG 6 oz wt/a DORMANT A 62.5 a 71.3 abc 85.0 ab 82.5 a 84.0 a 4 CERTAINTY 75 WG 1.25 oz wt/a DORMANT A 75.0 a 83.8 a 92.0 a 90.8 a 92.0 a 5 FLAZASULFURON 25 DG 1.5 oz wt/a 60%GREEN B 66.3 a 63.8 bcd 82.5 ab 85.0 a 90.8 a 6 FLAZASULFURON 25 DG 3 oz wt/a 60%GREEN B 63.8 a 55.0 cd 77.5 b 80.0 a 90.0 a 7 FLAZASULFURON 25 DG 6 oz wt/a 60%GREEN B 61.3 a 51.3 d 47.5 c 67.5 a 81.3 a 8 CERTAINTY 75 WG 1.25 oz wt/a 60%GREEN B 73.8 a 71.3 abc 90.0 ab 86.3 a 94.5 a 9 FLAZASULFURON 25 DG 1.5 oz wt/a 100% C 87.5 a 10 FLAZASULFURON 25 DG 3 oz wt/a 100% C 92.0 a 11 FLAZASULFURON 25 DG 6 oz wt/a 100% C 88.8 a 12 CERTAINTY 75 WG 1.25 oz wt/a 100% C 85.0 a 13 CHECK 67.5 a 75.0 ab 86.3 ab 83.8 a 88.8 a LSD (P=.05) Standard Deviation CV Bartlett's X P(Bartlett's X2) 0.006* * 0.09 Replicate F Replicate Prob(F) Treatment F Treatment Prob(F)

5 Pest Type Pest Code Crop Code AXOAF AXOAF AXOAF AXOAF AXOAF BBCH Scale BGWE BGWE BGWE BGWE BGWE Crop Name Narrow-leaf> Narrow-leaf> Narrow-leaf> Narrow-leaf> Narrow-leaf> Rating Date Rating Data Type COVERAGE COVERAGE COVERAGE INJURY INJURY Days After First/Last Applic Trt-Eval Interval 15 DA-C 22 DA-C 27 DA-C 15 DA-B 22 DA-B No. Name Conc Type Rate Unit Stage Code FLAZASULFURON 25 DG 1.5 oz wt/a DORMANT A 95.0 a 98.0 a 98.3 a 0.0 d 0.0 d 2 FLAZASULFURON 25 DG 3 oz wt/a DORMANT A 85.0 a 95.3 a 94.0 a 0.0 d 0.0 d 3 FLAZASULFURON 25 DG 6 oz wt/a DORMANT A 86.3 a 96.0 a 95.3 a 0.0 d 0.0 d 4 CERTAINTY 75 WG 1.25 oz wt/a DORMANT A 91.3 a 97.3 a 98.5 a 0.0 d 2.5 d 5 FLAZASULFURON 25 DG 1.5 oz wt/a 60%GREEN B 92.5 a 97.3 a 98.5 a 32.5 b 21.3 b 6 FLAZASULFURON 25 DG 3 oz wt/a 60%GREEN B 91.3 a 97.3 a 97.5 a 37.5 ab 37.5 a 7 FLAZASULFURON 25 DG 6 oz wt/a 60%GREEN B 87.5 a 97.3 a 97.5 a 40.0 a 45.0 a 8 CERTAINTY 75 WG 1.25 oz wt/a 60%GREEN B 92.5 a 98.0 a 98.3 a 20.0 c 12.5 c 9 FLAZASULFURON 25 DG 1.5 oz wt/a 100% C 87.5 a 94.5 ab 93.8 a 10 FLAZASULFURON 25 DG 3 oz wt/a 100% C 85.0 a 94.5 ab 94.0 a 11 FLAZASULFURON 25 DG 6 oz wt/a 100% C 83.8 a 91.3 b 93.5 a 12 CERTAINTY 75 WG 1.25 oz wt/a 100% C 87.5 a 94.5 ab 94.5 a 13 CHECK 90.8 a 97.3 a 96.8 a 0.0 d 0.0 d LSD (P=.05) Standard Deviation CV Bartlett's X P(Bartlett's X2) * Replicate F Replicate Prob(F) Treatment F Treatment Prob(F)

6 Pest Type Pest Code Crop Code AXOAF AXOAF AXOAF AXOAF AXOAF BBCH Scale BGWE BGWE BGWE BGWE BGWE Crop Name Narrow-leaf> Narrow-leaf> Narrow-leaf> Narrow-leaf> Narrow-leaf> Rating Date Rating Data Type INJURY INJURY INJURY INJURY INJURY Days After First/Last Applic Trt-Eval Interval 30 DA-B 36 DA-B 7 DA-C 15 DA-C 22 DA-C No. Name Conc Type Rate Unit Stage Code FLAZASULFURON 25 DG 1.5 oz wt/a DORMANT A 0.0 d 0.0 b 0.0 a 0.0 c 0.0 c 2 FLAZASULFURON 25 DG 3 oz wt/a DORMANT A 0.0 d 0.0 b 0.0 a 0.0 c 0.0 c 3 FLAZASULFURON 25 DG 6 oz wt/a DORMANT A 0.0 d 0.0 b 0.0 a 0.0 c 0.0 c 4 CERTAINTY 75 WG 1.25 oz wt/a DORMANT A 2.5 cd 1.3 b 2.5 a 0.0 c 0.0 c 5 FLAZASULFURON 25 DG 1.5 oz wt/a 60%GREEN B 11.3 bc 0.0 b 0.0 a 0.0 c 0.0 c 6 FLAZASULFURON 25 DG 3 oz wt/a 60%GREEN B 21.3 b 3.8 b 0.0 a 0.0 c 0.0 c 7 FLAZASULFURON 25 DG 6 oz wt/a 60%GREEN B 52.5 a 16.3 a 0.0 a 0.0 c 0.0 c 8 CERTAINTY 75 WG 1.25 oz wt/a 60%GREEN B 7.5 cd 0.0 b 0.0 a 0.0 c 0.0 c 9 FLAZASULFURON 25 DG 1.5 oz wt/a 100% C 2.5 a 8.8 b 0.0 c 10 FLAZASULFURON 25 DG 3 oz wt/a 100% C 0.0 a 15.0 a 1.3 b 11 FLAZASULFURON 25 DG 6 oz wt/a 100% C 2.5 a 17.5 a 5.0 a 12 CERTAINTY 75 WG 1.25 oz wt/a 100% C 0.0 a 3.8 c 0.0 c 13 CHECK 0.0 d 0.0 b 0.0 a 0.0 c 0.0 c LSD (P=.05) Standard Deviation CV Bartlett's X P(Bartlett's X2) * Replicate F Replicate Prob(F) Treatment F Treatment Prob(F)

7 Pest Type W Weed W Weed W Weed W Weed Pest Code POAAN POAAN POAAN POAAN Crop Code AXOAF BBCH Scale BGWE Crop Name Narrow-leaf> Rating Date Rating Data Type INJURY INJURY INJURY CONTROL CONTROL Days After First/Last Applic Trt-Eval Interval 27 DA-C 14 DA-A 21 DA-A 29 DA-A 33 DA-A No. Name Conc Type Rate Unit Stage Code FLAZASULFURON 25 DG 1.5 oz wt/a DORMANT A 0.0 a 50.0 a 77.5 b 72.5 ab 78.8 a 2 FLAZASULFURON 25 DG 3 oz wt/a DORMANT A 0.0 a 65.0 a 90.0 a 78.8 ab 83.8 a 3 FLAZASULFURON 25 DG 6 oz wt/a DORMANT A 0.0 a 63.3 a 90.0 a 95.0 a 95.8 a 4 CERTAINTY 75 WG 1.25 oz wt/a DORMANT A 0.0 a 48.8 a 73.8 b 66.3 b 71.3 a 5 FLAZASULFURON 25 DG 1.5 oz wt/a 60%GREEN B 0.0 a 6 FLAZASULFURON 25 DG 3 oz wt/a 60%GREEN B 0.0 a 7 FLAZASULFURON 25 DG 6 oz wt/a 60%GREEN B 0.0 a 8 CERTAINTY 75 WG 1.25 oz wt/a 60%GREEN B 0.0 a 9 FLAZASULFURON 25 DG 1.5 oz wt/a 100% C 0.0 a 10 FLAZASULFURON 25 DG 3 oz wt/a 100% C 0.0 a 11 FLAZASULFURON 25 DG 6 oz wt/a 100% C 0.0 a 12 CERTAINTY 75 WG 1.25 oz wt/a 100% C 0.0 a 13 CHECK 0.0 a 0.0 b 0.0 c 0.0 c 0.0 b LSD (P=.05) Standard Deviation CV Bartlett's X P(Bartlett's X2) * 0.028* Replicate F Replicate Prob(F) Treatment F Treatment Prob(F)

8 Pest Type W Weed W Weed W Weed W Weed W Weed Pest Code POAAN POAAN POAAN POAAN POAAN Crop Code BBCH Scale Crop Name Rating Date Rating Data Type CONTROL CONTROL CONTROL CONTROL CONTROL Days After First/Last Applic Trt-Eval Interval 7 DA-B 15 DA-B 22 DA-B 30 DA-B 36 DA-B No. Name Conc Type Rate Unit Stage Code FLAZASULFURON 25 DG 1.5 oz wt/a DORMANT A 97.5 a 85.0 ab a 96.3 ab 92.5 a 2 FLAZASULFURON 25 DG 3 oz wt/a DORMANT A 98.8 a 98.8 a a a a 3 FLAZASULFURON 25 DG 6 oz wt/a DORMANT A a a a a a 4 CERTAINTY 75 WG 1.25 oz wt/a DORMANT A a 91.3 a 87.5 a 90.0 b 86.3 a 5 FLAZASULFURON 25 DG 1.5 oz wt/a 60%GREEN B 13.8 b 66.3 b 83.3 ab 97.5 ab 98.8 a 6 FLAZASULFURON 25 DG 3 oz wt/a 60%GREEN B 7.5 c 82.5 ab 86.3 ab 99.5 a a 7 FLAZASULFURON 25 DG 6 oz wt/a 60%GREEN B 10.0 bc 91.3 a 94.3 a a a 8 CERTAINTY 75 WG 1.25 oz wt/a 60%GREEN B 7.5 c 43.8 c 61.3 b a 86.3 a 9 FLAZASULFURON 25 DG 1.5 oz wt/a 100% C 10 FLAZASULFURON 25 DG 3 oz wt/a 100% C 11 FLAZASULFURON 25 DG 6 oz wt/a 100% C 12 CERTAINTY 75 WG 1.25 oz wt/a 100% C 13 CHECK 0.0 d 0.0 d 0.0 c 0.0 c 0.0 b LSD (P=.05) Standard Deviation CV Bartlett's X P(Bartlett's X2) * 0.006* 0.003* 0.014* Replicate F Replicate Prob(F) Treatment F Treatment Prob(F)

9 Pest Type W Weed W Weed W Weed W Weed W Weed Pest Code SISBE SISBE SISBE SISBE SISBE Narrowleaf > Narrowleaf > Crop Code BBCH Scale Crop Name Rating Date Rating Data Type INJURY CONTROL CONTROL CONTROL CONTROL Days After First/Last Applic Trt-Eval Interval 21 DA-A 29 DA-A 33 DA-A 7 DA-B 15 DA-B No. Name Conc Type Rate Unit Stage Code FLAZASULFURON 25 DG 1.5 oz wt/a DORMANT A 75.0 a 98.8 ab 98.8 a a a 2 FLAZASULFURON 25 DG 3 oz wt/a DORMANT A 75.0 a 98.8 ab a a a 3 FLAZASULFURON 25 DG 6 oz wt/a DORMANT A 75.0 a a a a a 4 CERTAINTY 75 WG 1.25 oz wt/a DORMANT A 75.0 a 95.0 b 92.5 b a a 5 FLAZASULFURON 25 DG 1.5 oz wt/a 60%GREEN B 13.3 b 70.0 b 6 FLAZASULFURON 25 DG 3 oz wt/a 60%GREEN B 5.0 cd 60.0 b 7 FLAZASULFURON 25 DG 6 oz wt/a 60%GREEN B 7.5 c 60.0 b 8 CERTAINTY 75 WG 1.25 oz wt/a 60%GREEN B 6.7 c 41.7 c 9 FLAZASULFURON 25 DG 1.5 oz wt/a 100% C 10 FLAZASULFURON 25 DG 3 oz wt/a 100% C 11 FLAZASULFURON 25 DG 6 oz wt/a 100% C 12 CERTAINTY 75 WG 1.25 oz wt/a 100% C 13 CHECK 0.0 b 0.0 c 0.0 c 0.0 d 0.0 d LSD (P=.05) Standard Deviation CV Bartlett's X P(Bartlett's X2) Replicate F Replicate Prob(F) Treatment F Treatment Prob(F)

10 Pest Type W Weed W Weed W Weed Pest Code SISBE SISBE SISBE Crop Code BBCH Scale Crop Name Rating Date Rating Data Type CONTROL CONTROL CONTROL Rating Unit PERCENT PERCENT PERCENT L.S. WARREN L.S. WARREN L.S. WARREN Days After First/Last Applic Trt-Eval Interval 15 DA-B 30 DA-B 36 DA-B ARM Action Codes P P P No. Name Conc Type Rate Unit Stage Code FLAZASULFURON 25 DG 1.5 oz wt/a DORMANT A a a a 2 FLAZASULFURON 25 DG 3 oz wt/a DORMANT A a a a 3 FLAZASULFURON 25 DG 6 oz wt/a DORMANT A a a a 4 CERTAINTY 75 WG 1.25 oz wt/a DORMANT A a a a 5 FLAZASULFURON 25 DG 1.5 oz wt/a 60%GREEN B 98.0 b a a 6 FLAZASULFURON 25 DG 3 oz wt/a 60%GREEN B 97.5 b a a 7 FLAZASULFURON 25 DG 6 oz wt/a 60%GREEN B 99.0 ab a a 8 CERTAINTY 75 WG 1.25 oz wt/a 60%GREEN B 97.8 b a a 9 FLAZASULFURON 25 DG 1.5 oz wt/a 100% C 10 FLAZASULFURON 25 DG 3 oz wt/a 100% C 11 FLAZASULFURON 25 DG 6 oz wt/a 100% C 12 CERTAINTY 75 WG 1.25 oz wt/a 100% C 13 CHECK 0.0 c 0.0 b 0.0 b LSD (P=.05) Standard Deviation CV Bartlett's X P(Bartlett's X2) Replicate F Replicate Prob(F) Treatment F Treatment Prob(F) Trial Comments : AXOAF WAS 90-95% DORMANT WITH NO DETECTABLE INJURY SYMPTOMS AT THIS TIME