Supplement of Population-specific responses in physiological rates of Emiliania huxleyi to a broad CO 2 range

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1 Supplement of Biogeosciences, 15, , 21 Author(s) 21. This work is distributed under the Cretive Commons Attribution. License. Supplement of Popultion-specific responses in physiologicl rtes of Emilini huxleyi to brod CO 2 rnge Yong Zhng et l. Correspondence to: Yong Zhng (zhngyong193@xmu.edu.cn) The copyright of individul prts of the supplement might differ from the CC BY. License.

2 Tble S1. Se surfce temperture (SST) t the Azores, Bergen nd Cnry Islnds. Men monthly Min. monthly Mx. monthly SST rnge ( o C) SST ( o C) SST ( o C) References Azores Wisshk et l., 21 Bergen Smuelsen, 197 Locrnini et l., 26 Cnry Islnds Sntn-Csino et l., 27

3 Tble S2. Number of bse pirs (bp) in the exmined loci (mrked with sterisk) in the lleles of individul genotypes. The numbers of bse pirs in ll exmined loci re different, which indictes different genotypes within popultion. NA indictes tht no lleles could be obtined. Genotype P2E9 * P2B * P2F11 * EHMS37 * EHMS15 * A A A A A A B B B B B B NA NA C C C NA NA C NA NA C NA NA

4 Tble S3. Clculted optimum, mximum vlue (V mx ) nd reltive sensitivity (rs, ) of POC nd PIC quots of ech E. huxleyi strin. POC quot PIC quot optimum V mx (pg rs optimum V mx (pg rs Strin (µtm) C cell 1 ) (µtm) C cell 1 ) A A A A A A B B B B B B C C C C C

5 the Azores strins A23, A22, A21, A19, A13, A1 the Bergen strins B95, B63, B62, B51, B1, B17 the Cnry Islnds strins C9, C91, C9, C1, C A23 A22 A21 A19 A13 A1 B95 B63 B62 B51 B1 B17 C9 C91 C9 C1 C35 Ech strin ws incubted for to 6 dys t 11 CO 2 levels rnging from 115 to 37 μtm with no replicte At the end of the incubtions, growth, POC nd PIC production rtes were mesured Figure S1. A flow chrt for the experimentl processes.

6 PIC quot (pg C cell 1 ) POC quot (pg C cell 1 ) reltive PIC quot (%) reltive POC quot (%) () 1 1 (c) Azores Bergen Cnry Islnds 6 2 (b) 1 1 (d) 6 2 ( tm) ( tm) Figure S2. Optimum curve responses of POC nd PIC quots of three E. huxleyi popultions to rnge from µtm to 263 µtm. Responses of POC quot () nd PIC quot (b) to. Responses of reltive POC quot (c) nd reltive PIC quot (d) to. For more detil informtion, see Figure 1.

7 optimum of PIC quot ( tm) mximum PIC quot (pg C cell 1 ) reltive sensitivity of PIC quot ( ) optimum of POC quot ( tm) mximum POC quot (pg C cell 1 ) reltive sensitivity of POC quot ( ) clculted optimum () clculted mximum vlue (c) b fitted reltive sensitivity b (e) b 3 Azores Bergen Cnry Islnds 3 Azores Bergen Cnry Islnds. Azores Bergen Cnry Islnds (b) (d).9.6 (f) Azores Bergen Cnry Islnds Azores Bergen Cnry Islnds. Azores Bergen Cnry Islnds Figure S3. Clculted optimum, clculted mximum vlue nd fitted reltive sensitivity constnt of POC nd PIC quots of ech popultion. () optimum of POC quot; (b) optimum of PIC quot; (c) mximum POC quot; (d) mximum PIC quot; (e) reltive sensitivity constnt of POC quot; (f) reltive sensitivity constnt of PIC quot. For more detil informtion, see Figure 2.

8 PIC quot (pg C cell 1 ) POC quot (pg C cell 1 ) Azores Bergen Cnry Islnds A23 A22 A21 A19 A13 A1 () B95 B63 B62 B51 B1 B17 (b) (c) C9 C91 C9 C1 C35 (d) (e) (f) ( tm) ( tm) ( tm) Figure S. Optimum curve responses of POC nd PIC quots of individul E. huxleyi strins in the Azores (left), Bergen (medium) nd Cnry Islnds (right) popultions to CO 2 rnge from 115 µtm to 37 µtm. POC quot of ech strin s function of within the Azores (), Bergen (b) nd Cnry Islnds (c) popultions. PIC quot of ech strin s function of within the Azores (d), Bergen (e) nd Cnry Islnds (f) popultions. For more detil informtion, see Figure 3.

9 growth rte (d 1 ) growth rte (d 1 ) () y =.25 * x +.1 R 2 = POC production rte (pg C cell 1 d 1 ) (b) y =.13 * x R 2 = POC quot (pg C cell 1 ) Figure S5. Incresed growth rtes of 17 E. huxleyi strins with incresing POC production rtes () or POC quots (b).

10 PIC : POC rtio 2. Azores Bergen Cnry Islnds ( tm) Figure S6. Responses of PIC : POC rtio of the Azores (squre), Bergen (circulr) nd Cnry Islnds (dimond) popultions to CO 2 rnge from µtm to 263 µtm.

11 PIC : POC rtio PIC : POC rtio PIC : POC rtio A23 A22 A21 A19 A13 A1 () (b).9.6 B95 B63 B62 B51 B1 B (c) C9 C91 C9 C1 C35 1 ( tm) Figure S7. Responses of PIC : POC rtio of individul E. huxleyi strins in the Azores (), Bergen (b) nd Cnry Islnds (c) popultions to CO 2 rnge from 115 µtm to 37 µtm.

12 References Locrnini, R. A., Mishonov, A. V., Antonov, J. I., Boyer, T. P., nd Grci, H. E.: World ocen tls 25, V. 1: Temperture, in: NOAA Atls NESDIS 61, edited by Levitus, S., U.S. Government Printing Office, 3 13, 26. Smuelsen, T. J.: The biology of six species of Anomur (Crustce, Decpod) from Runefjorden, western Norwy, Srsi, 5, 25 52, 197. Sntn-Csino, J. M., González-Dávil, M., Rued, M., Llinás, O., nd González-Dávil, E.: The internnul vribility of ocenic CO 2 prmeters in the northest Atlntic subtropicl gyre t the ESTOC site, Glob. Biogeochem. Cycles, 21, GB115, doi: 1.129/26GB27, 27. Wisshk, M., Form, A., Jkobsen, J., nd Freiwld, A.: Temperte crbonte cycling nd wter mss properties from intertidl to bthyl depths (Azores), Biogeosciences, 7, , doi: 1.519/bg , 21.

The Discussion of this exercise covers the following points: The open-loop Ziegler-Nichols method. The open-loop Ziegler-Nichols method

The Discussion of this exercise covers the following points: The open-loop Ziegler-Nichols method. The open-loop Ziegler-Nichols method Exercise 6-3 Level Process Control EXERCISE OBJECTIVE In this exercise, you will perform PID control of level process. You will use the open-loop step response method to tune the controller. DISCUSSION