EcoElectrica s 2016 Biological Monitoring Plan and Outreach Report

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1 EcoElectrica s 2016 Biological Monitoring Plan and Outreach Report Luis R. Rodríguez Matos Ernesto Otero Rosamar Ayala UPRM-Mayagüez

2 Summary Monthly Water Quality Sampling Continuous Water Quality Monitoring (YSI Sondes) Impingement Sedimentation Seagrass Habitat Mapping Fosforescent Bay Monitoring

3 Water Quality Monthly sampling of temperature, salinity, turbidity, phytoplankton and cyanobacterial biomass indicators (chlorophyll and phycoerythrin), and colored organic matter fluorescence. Continuous Monitoring of ph, turbidity, salinity, DO, temperature.

4 Study Area Water Quality Stations at Guayanilla Bay.

5 Turbidity (NTU) Turbidity Time Series Estacion 11 Estacion 12 Estacion 4 Estacion 4N Estacion 5 Estacion 8 Estacion Intake Estacion Outfall

6 Temperature C Temperature Time Series Estacion 11 Estacion 12 Estacion 4 Estacion 4N Estacion 5 Estacion 8 Estacion Intake Estacion Outfall 26.5

7 Chlorophyll (ug/l ) Chlorophyll Time series Estacion 11 Estacion 12 Estacion 4 Estacion 4N Estacion 5 Estacion 8 Estacion Intake Estacion Outfall

8 Phycoerythrin (RFU ) Phycoerythrin Time Series Estacion 11 Estacion 12 Estacion 4 Estacion 4N Estacion 5 Estacion 8 Estacion Intake Estacion Outfall 60

9 SALINITY Salinity Time Series Outfall Intake 12 4N Nov Oct Sep Aug Jul Jun May Apr Mar Feb Jan

10 YSI Sonde at the Intake. Data collected every 3 min for 14 days intervals

11 Dissilve Oxygen (ul/l) Continuous Monitoring at Intake and Outfall (YSI Sondes) 8.5 Average Dissolve Oxygen Outfall Intake

12 Turbidity (NTU) Continuous Monitoring at Intake and Outfall (YSI Sondes) 1000 Average Turbidity Outfall Intake

13 Impingement

14 Number of Individual Impingement Impingement Day Time Survey January February March April June July August October November December Sampling Month Atlantic bumper Atlantic Spadefish Atlantic Surgeonfish Black Margate Blue Head Wrasse Blue Tang Dog Snapper Foureye Butterflyfish Grey Snapper Horse-Eye Jack Ocean Surgeonfish Porkfish Queen Angelfish Sargent major School master Snapper Surgeon Fish Tarpoon Yellow tail Snapper

15 Number of Individual Impingement Impingement Night Time Survey January February March April June July August October November December Sampling Month Atlantic bumper Atlantic Spadefish Atlantic Surgeonfish Black Margate Blue Head Wrasse Blue Tang Dog Snapper Foureye butterfly fish Grey Snapper Horse-Eye Jack Ocean Surgeonfish Porkfish Queen Angelfish Sargent major School master Snapper Surgeon Fish Tarpoon Yellow tailsnapper

16 Sedimentation Apr-14 7-Apr-14 2-May-14 5-May-14 5-Dec Apr Aug Sep-16 4N EAST INTAKE OUTFALL WEST

17 Seagrass Mapping Benthic photographs were taken along 50 m long transects, m apart from each other and oriented perpendicularly to the pier. Photographs were taken using a surface deployed quadrat and camera array in navigable waters. Photographs were taken while snorkeling when precise navigation was not possible due currents, depth or unsafe navigation conditions were present (i.e. too shallow). Photographs were geotagged by synchronizing GPS coordinates collected during sampling and a specialized geotagging software (RoboGeo). Seagrass Cover was estimated at each site using Coral Point Counter software (CPCe). Seagrass cover countoures were created using Grapher (Golden Software)

18 Seagrass Mapping

19 Seagrass Mapping

20 Seagrass Mapping

21 Seagrass Mapping

22 Seagrass Mapping

23 Seagrass Mapping

24 Seagrass Mapping

25 Seagrass Mapping

26 Seagrass Mapping

27 Seagrass Mapping

28 Seagrass Mapping

29 Seagrass Mapping

30 Outreach: Fosforescent Bay

31 Chlorophyll Time Series at Fosforescent Bay Station 1 Station 2 Station 3 Station 4 Station 5 Station 6 Station Jul 18-Aug 7-Sep 27-Sep 17-Oct 6-Nov 26-Nov

32 Bioluminescence Levels Bahía Fosforescente 2.50E E E E+12 Estacion 1 Estación 2 Estación 3 Estacion 4 Estación 5 Estación E E Jul 18-Aug 7-Sep 27-Sep 17-Oct 6-Nov 26-Nov 16-Dec 5-Jan

33 Pyrodinium bahamense abundance Estacion 1 Estación 2 Estación 3 Estacion 4 Estación 5 Estación Jul 18-Aug 7-Sep 27-Sep 17-Oct 6-Nov 26-Nov 16-Dec 5-Jan

34 Future Impingement An extension to corroborate seasonality with better intervalometers and light sources.

35 Sedimentation Results from trace element (i.e. Cu, As, Pb) contents of sediments from traps. Samples were sent and analytical results are expected to arrive during January Evaluate the possibility of trace element transport along suspended sediments in Guayanilla Bay.

36 Seagrasses Complete sampling and analysis of seagrass distribution close to EcoElectricas pier at unsampled sites. Evaluate the temporal distribution of seagrasses at sites close to the Outfall Station.

37 Water Quality Continuous Analysis of Water Quality at the Intake and Outfall Sites Monthly sampling of other sites. Intercalibration between sampling strategies. Intercomparison with previous studies.

38 Expand monitoring to include determinations of the appearance of subsurface oxygen depletion that may affect the production of bioluminescence Evaluate alternative means of bioluminescence determination using economic photographic approaches. These may be suitable to expand stakeholders monitoring. Fosforescent Bay

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