CHEMICAL HYGIENE PLAN CHEMISTRY LABORATORY WATER SCIENCES SECTION 4405 REEDY CREEK ROAD NORTH CAROLINA DIVISION OF WATER RESOURCES 2015 REVISION

Transcription

1 DWR-Labratry Safety CHEMICAL HYGIENE PLAN CHEMISTRY LABORATORY WATER SCIENCES SECTION 4405 REEDY CREEK ROAD RALEIGH, NC NORTH CAROLINA DIVISION OF WATER RESOURCES 2015 REVISION Effective Date: Octber 1, f 66 Revised:

2 DWR-Labratry Safety TABLE OF CONTENTS - Title Page... 1 Table f Cntents... 2 Revisin Histry Tracking Frm Overview CHP Applicability Respnsibilities f Emplyees Accident / Incident Reprting WSS Chemistry Labratry Safety Cmmittee CHP and Safety Training CHP Medical Surveillance Visitrs t the WSS Chemistry Labratry CHP Review and Update CHP Emergency Respnse Persnal Prtective Equipment First Aid Wrk Area Safety and Husekeeping Labratry Fire Safety Cmpressed Gases Electrical & Mechanical Hazards Vacuum Autclaves Labratry Ventilatin Bilgical Materials Hazards Radiactive Materials Hazards Particularly Hazardus Substances Chemical Waste Management Bilgical Waste Management Frms Appendix Certificatin f Unit Training.. 42 New Emplyee Safety Orientatin & Training CHP Orientatin Training Emergency Ntificatin Frm Fire Drill Checklist Fume Hd Infrmatin Fume Hd Evaluatin Wrksheet...48 Organic Slvent Waste Lg Slvent Waste Drums - Weekly Inspectin Lg.. 50 Grund Flr and Emergency Evacuatin Plan List f Particularly Hazardus Substances Appendix A: Labratry Chemical Spill Plan.53 Appendix B: Spill-X Agents - Standard Operating Prcedures...60 Appendix C: Mercury Spill Cleanup using the Mercn Spill Kit - Standard Operating Prcedures 63 Appendix D: Glves and Chemical Resistance...65 Effective Date: Octber 1, f 66 Revised:

3 DWR-Labratry Safety Revisin Histry Tracking Frm: CHP Revisin Date Reviewed r Mdified? Cmments 9/2015 R-C. Jhnsn, J. Park, M. Overman M-M. Overman, C. Jhnsn Revised sectin 4; Revised sectin 10 and added 3 appendices (A-C) fr guidance n chemical spills; Added appendix D fr infrmatin n glves and chemical resistance; Added ergnmics t sectin 13; Added Knx Bx t sectin 14; Changed DWR Safety Officer t Department Safety Directr r Office. 9/2015 R-Chris Cangemi (DEQ) Final review and apprval DEQ Safety Office. Effective Date: Octber 1, f 66 Revised:

4 DWR-Labratry Safety 1 CHEMICAL HYGIENE PLAN OVERVIEW In the daily perfrmance f duties in the Water Sciences Sectin (WSS) Chemistry Labratry, it may be necessary t wrk with hazardus chemicals and equipment. This may include: flammable, crrsive, explsive, carcingenic r txic chemicals bilgical hazards Physical hazards such as high pressure gas cylinders, temperature extremes r high vltage equipment The (CHP) fr the WSS Chemistry Labratry sets frth the Divisin f Water Resurces (DWR) safe labratry practices (as part f the Nrth Carlina Department f Envirnmental Quality). Details f the safe use f chemicals and equipment are als included in the standard perating prcedure fr each unit f the Chemistry Labratry. All labratry emplyees are required t be familiar with this. As with any wrkplace in DWR, labratry emplyees shuld knw: The nearest building exit, the evacuatin rute t that exit and the utside assembly area. The lcatin f safety equipment in their lab unit. The lcatins f fire alarm pull statins and fire extinguishers. The lcatin f the Safety Data Sheets (SDS) file fr their lab unit. 2 CHP APPLICABILITY The OSHA standard that gverns use f r handling f hazardus chemicals in a labratry setting is the Occupatinal Expsure t Hazardus Chemicals in Labratries lab standard 29 CFR It nly applies t labratry use f hazardus chemicals in which all f the fllwing criteria are met: Prcedures using chemicals are carried ut using cntainers that are easily handled by ne persn (i.e., manipulatins are carried ut n a labratry scale). Multiple chemical prcedures r multiple chemicals are used. Operatins invlved are nt part f a prductin prcess, nr in any way simulate a prductin prcess. Prtective practices and equipment are available and cmmnly used t minimize the ptential fr emplyee expsure t hazardus chemicals. Belw is a general summary f the elements f the Chemical Hygiene Standard: (e) - General (1) Where hazardus chemicals as defined by this standard are used in the wrkplace, the emplyer shall develp and carry ut the prvisins f a written which is: (i) Capable f prtecting emplyees frm health hazards assciated with hazardus chemicals in that labratry and (ii) Capable f keeping expsures belw the limits specified in paragraph(c) f this sectin (29 CFR part 1910, subpart Z). (2) The shall be readily available t emplyees and emplyee representatives. Effective Date: Octber 1, f 66 Revised:

5 DWR-Labratry Safety (3) The shall include each f the fllwing elements and shall indicate specific measures that the emplyer will take t ensure labratry emplyee prtectin; (i) Standard perating prcedures relevant t safety and health cnsideratins t be fllwed when labratry wrk invlves the use f hazardus chemicals (ii) Criteria that the emplyer will use t determine and implement cntrl measures t reduce emplyee expsure t hazardus chemicals including engineering cntrls, the use f persnal prtective equipment and hygiene practices; particular attentin shall be given t the selectin f cntrl measures fr chemicals that are knwn t be extremely hazardus (iii) A requirement that fume hds and ther prtective equipment are functining prperly and specific measures that shall be taken t ensure prper and adequate perfrmance f such equipment (iv) Prvisins fr emplyee infrmatin and training as prescribed in paragraph (f) f this sectin (v) The circumstances under which a particular labratry peratin, prcedure r activity shall require prir apprval frm the emplyer r the emplyer's designee befre implementatin (vi) Prvisins fr medical cnsultatin and medical examinatins in accrdance with paragraph (g) f this sectin (vii) Designatin f persnnel respnsible fr implementatin f the including the assignment f a Chemical Hygiene Officer, and, if apprpriate, establishment f a Chemical Hygiene Cmmittee (viii) Prvisins fr additinal emplyee prtectin fr wrk with particularly hazardus substances. These include "select carcingens," reprductive txins and substances which have a high degree f acute txicity. Specific cnsideratin shall be given t the fllwing prvisins which shall be included where apprpriate: Establishment f a designated area Use f cntainment devices such as fume hds r glve bxes Prcedures fr safe remval f cntaminated waste Decntaminatin prcedures. (4) The emplyer shall review and evaluate the effectiveness f the at least annually and update it as necessary. 3 RESPONSIBILITIES OF EMPLOYEES The WSS Chemistry Labratry intends t fully cmply with 29 CFR , which is knwn as the Occupatinal Expsure t Hazardus Chemicals in Labratries standard and any amendments specific fr the State f Nrth Carlina. Every WSS Chemistry Labratry emplyee has the respnsibility t ensure the plicies as set frth in this prgram are carried ut. T ensure this gal, the fllwing respnsibilities are assigned: 3.1 Water Sciences Sectin Chief WSS Chief is Cyndi Karly: Street: 4401 Reedy Creek Rad, Raleigh, NC Effective Date: Octber 1, f 66 Revised:

6 DWR-Labratry Safety Mail: 1623 Mail Service Center, Raleigh, NC Telephne: The verall respnsibility fr Chemistry Labratry peratins rests with the Water Sciences Sectin chief. The Sectin chief shall: Assist the Chemical Hygiene Officer and the Department Safety Office in develping lng range safety and health gals fr WSS Chemistry Labratry emplyees. Prvide the funding necessary t achieve the safety and health gals, including medical surveillance, persnal prtective equipment, and upgrades t engineering cntrls. Set an example fr safety at all times while in the emply f this Divisin. 3.2 WSS Chemistry Labratry Chemical Hygiene Officer WSS Chemistry Labratry CHO - Chris Jhnsn: Street: 4405 Reedy Creek Rad, Raleigh, NC Mail: 1623 Mail Service Center, Raleigh, NC Telephne: , extensin 224. DEQ Safety Directr - Mike Cnnr: Street: 217 W Jnes Street, Green Square Building, Raleigh, NC Mail: 1607 Mail Service Center, Raleigh NC Telephne: (ffice); (mbile) The Chemical Hygiene Officer (CHO) is the primary persn respnsible fr ensuring that the CHP is implemented thrughut the WSS Chemistry Labratry. The majr respnsibilities f the CHO are t: Prvide technical assistance in cmplying with the and answering questins cncerning safety fr emplyees. Assist Labratry managers and supervisrs in develping apprpriate safety precautins and prcedures fr the lab units. Develp and maintain a system f cllectin and dispsal f chemical wastes, and mnitr the Chemical Waste Dispsal prgram. Prvide t the Department Safety Directr an analysis f any chemical spill r accident within 48 hurs f the incident, and develp prpsed changes in prcedures r plicies t imprve emplyee safety and decrease the risk f similar ccurrence. Maintain a dcumentatin prgram that includes all accident, injury, incident, inspectin, and hd maintenance recrds. In cnjunctin with the Department Safety ffice, the CHO will: Assess pssible cases f ver-expsure, and determine when an expsure assessment is warranted. Establish lng-range safety and health perfrmance gals. Cmmunicate with the WSS Chief t discuss safety issues and pririties, annual gals and bjectives, funding needs, and lng-range plans fr imprvement. Crdinatin f safety training fr WSS Chemistry Labratry emplyees. Effective Date: Octber 1, f 66 Revised:

7 DWR-Labratry Safety Perfrm rutine safety inspectins f the Labratry facilities. 3.3 Managers and Supervisrs The WSS Chemistry Labratry managers and supervisrs are respnsible fr ensuring this Plan is carried ut at the branch and unit level. At a minimum, managers and supervisrs are respnsible fr: Prviding safe wrking cnditins fr all Branch/Unit emplyees. Knwing and implementing the safety and health standards r regulatins applicable t the Branch r Unit. Ensuring that Standard Operating Prcedures (SOP s) fr their lab unit(s) include any additinal safety infrmatin fr chemicals, equipment, and prcesses that are specific t sample preparatins and analyses cnducted in the lab unit. Investigating any emplyee reprts f unsafe wrking cnditins r practices. Investigating and reprting accidents r incidents prmptly (by the end f the wrk shift) t the Water Sciences Sectin chief, the WSS Chemistry Labratry Safety Cmmittee, and the Divisin Safety Cnsultant. Ensuring that persnal prtective equipment is available as needed and ensuring that users are adequately trained in its use, care, and strage. 3.4 All Emplyees While in the emply f the Divisin f Water Resurces, each emplyee has a respnsibility t wrk in as safe a manner as pssible. In additin, all emplyees will: Refrain frm any unsafe act that might create dangerus cnditins. Use prescribed safety equipment and persnal prtective equipment as indicated in this plan and in their lab unit s standard perating prcedures (SOPs). Be aware f hazardus chemicals in the lab unit; read bttle labels, which prvide the mst visible and easily accessible safety infrmatin fr a chemical. Cnsult the Safety Data Sheet (SDS) fr each chemical used in the lab unit. An SDS prvides imprtant infrmatin including: Hazards Identificatin, First Aid and Fire-Fighting measures, Handling and Strage, Persnal Prtectin Equipment, Stability and Reactivity, and Txiclgical and Regulatry infrmatin. NOTE: Safety Data Sheet (SDS) previusly knwn as Material Safety Data Sheets (MSDS). Reprt ANY unsafe cnditin r act f a cwrker t their immediate supervisr, r t the Chemical Hygiene Officer. 4 ACCIDENT / INCIDENT REPORTING Any ccupatinal injury, accident r near-miss incident shuld be reprted as sn as pssible t the emplyee s supervisr (and the Labratry CHO). T call fr lcal Emergency Medical Services (EMS) frm a lab telephne, dial: Fllwing an accident r injury, the lab unit supervisr and the Labratry Safety Cmmittee shuld investigate the incident. The investigatin shuld determine and dcument the cause f the accident/injury, Effective Date: Octber 1, f 66 Revised:

8 DWR-Labratry Safety and whether any mdificatins t wrk prcedures need t be instituted t reduce the chances f repeat incidents. Reprtable accidents and injuries are t be entered int the Divisin f Water Resurces OSHA 300 frm, which is maintained by the Divisin Human Resurces Office. The OSHA 300 frm fr the WSS Chemistry Labratry, fr the preceding year, is t be psted n the emplyee bulletin bard lcated in the main hallway (if there were reprtable injuries). The OSHA 300 frm will be prvided by the DEQ Safety Office. The fllwing sectins prvide prcedures t fllw in case f a wrk-related accident r injury. 4.1 Nn-Fatal Injuries, Accidents and Near-Miss Incidents All accidents and injuries, and near-miss incidents, must be reprted as sn as pssible t the emplyee s supervisr. The supervisr and Labratry CHO shuld assist in determining the severity f the injury r accident, and whether prfessinal medical attentin is needed and whether emergency persnnel shuld be cntacted. *Nte that fr an accident r injury (except multi-persn injuries r fatalities) that ccurs after nrmal wrk hurs r n a weekend r state hliday, the emplyee shuld attempt t cntact their supervisr and/r the Labratry CHO by telephne. If that is nt pssible, ntify the supervisr n the next wrk day. (1) Accident r Near-Miss Incident: Fr any wrk-place accidents r near-miss incidents that ccur and culd have resulted in an injury, the circumstances and details f the accident r incident, as well as the wrk prcess invlved, must be dcumented and investigated. Cmplete the accident/near-miss investigatin frms utlined in sectin 4.4. (2) Minr injury that des nt require prfessinal medical attentin: First-aid kits are available in the labratry fr treating minr injuries such as cuts, scrapes, and small burns. The Labratry CHO can assist with treatment. Cmplete the accident/near-miss investigatin frms utlined in sectin 4.4. (3) Injury is nt serius r life-threatening but requires prfessinal medical attentin: Prvide initial first aid treatment in the labratry. Transprt the injured emplyee t ne f the fllwing facilities: Accent Urgent Care, 2406 Blue Ridge Rad, Suite 190, telephne Rex Hspital, emergency rm, 4420 Lake Bne Trail, telephne: ; Imprtant: Ntify the treating facility that the injury is a Wrker s Cmpensatin case. Take 2 cpies f the Medical Authrizatin and Pharmacy frm (see sectin 4.3). *Only transprt an injured emplyee if it will nt aggravate the injury. If the mvement r transprt f an injured emplyee might aggravate the injury, cntact EMS and request an ambulance. Effective Date: Octber 1, f 66 Revised:

9 DWR-Labratry Safety Fllw prcedures fr Wrker s Cmpensatin as utlined in sectin 4.3. Cmplete the accident/near-miss investigatin frms utlined in sectin 4.4. (4) Injury is serius r life-threatening: Immediately cntact lcal EMS by dialing 911 (dial if using a lab telephne). If there is any dubt as t the severity f the injury, please cntact EMS persnnel. The persn cntacting EMS shuld prvide any infrmatin requested by the EMS peratr, e.g., the physical address f the labratry building (4405 Reedy Creek Rad), the nature f the accident and/r injury, and the cnditin and number f peple injured. The caller shuld remain n the telephne line until therwise instructed by the EMS peratr. If pssible (r if directed by EMS peratr), prvide initial first aid in the labratry. Imprtant: Prvide a cpy f the injured emplyee s Emergency Ntificatin Frm t EMS persnnel. Fllw prcedures fr Wrker s Cmpensatin as utlined in sectin 4.3. Cmplete the accident/near-miss investigatin frms utlined in sectin Accidents Invlving Multiple-Emplyee Injuries, Hspitalizatin, Amputatin, Lss f Eye(s), r Fatalities The Nrth Carlina Divisin f Occupatinal Safety and Health requires that the fllwing be reprted t NC OSH immediately: Accidents invlving a fatality Accidents invlving an emplyee s lss f limb(s) r eye(s) Hspitalizatin f an injured state emplyee Accidents invlving injuries t 3 r mre peple If an accident meets the abve criteria, fllw the steps belw: (1) Call DWR Human Resurces (Wanda Shacklefrd, telephne: ). (2) Call DEQ Safety Directr (Mike Cnnr, telephne: ). (3) During regular wrking hurs (8 a.m. t 5 p.m.), call the NC Department f Labr at r (4) Outside f regular wrking hurs, r n weekends r hlidays, call State Capitl Plice at (5) Call a member f the NC OSHR Safety and Health Divisin (see belw). Be prepared t prvide cntact infrmatin, addresses, and telephne numbers fr the persn(s) invlved. Jhn Bgner, telephne: Dug Gaylrd, telephne: Kathy Cnnr, telephne Effective Date: Octber 1, f 66 Revised:

10 DWR-Labratry Safety NC OSHR: Main Telephne: ; Fax: (6) Fllw-Up with an r fax t the ne f the abve NC OSHR staff. (7) The NC Office f State Human Resurces will ntify the Gvernr s Office and assist in investigatin f the incident. 4.3 Wrker s Cmpensatin Prcedures The State Gvernment Wrkers' Cmpensatin Prgram is administered and managed by the Office f State Human Resurces. The purpse f the prgram is t ensure that all eligible emplyees wh experience a wrk-related injury r illness receive apprpriate medical care and equitable benefits as prvided under the Wrkers' Cmpensatin Act and the State Human Resurces Plicy. The State is a self-insured emplyer and has cntracted with a Third Party Administratr (TPA) t handle the wrkers' cmpensatin claims f mst emplyees. The TPA is respnsible fr all cmpensatin and medical bill payments thrugh a wrkers' cmpensatin fund established by State agencies and universities and administered by the Office f the State Cntrller, in cperatin with the Office f State Human Resurces. The Labratry CHO and the WSS Chief shuld be ntified f any wrker s cmpensatin cases. The fllwing frms and prcedures are required fr Wrker s Cmpensatin cases: (1) Medical Authrizatin and Pharmacy frm: One cpy shuld be given t the attending physician. The physician shuld prvide the requested physical capabilities infrmatin n the frm. The cmpleted frm shuld be submitted t the emplyee s supervisr and the DWR Human Resurces Office. A secnd cpy is fr a pharmacy, if needed, and includes a list f authrized pharmacies where prescribed medicatins may be btained at n cst t the injured emplyee. (2) Nrth Carlina Industrial Cmmissin Frm 19 frm (titled Emplyer s Reprt f Emplyee s Injury r Occupatinal Disease t the Industrial Cmmissin ): Required by the Wrker s Cmpensatin Act. Respnsible Party: The injured emplyee s immediate supervisr. Time-Frame: Fill ut and submit frm within 24 hurs f the accident. Submit frm t: DWR Human Resurces Office (Wanda Shacklefrd) 1617 Mail Service Center Raleigh, Nrth Carlina Phne: FAX: r the DEQ Wrker s Cmpensatin Administratr (Chris Cangemi) 217 W. Jnes Street 5 th Flr 1607 Mail Service Center Raleigh, NC Office/Fax: (919) Effective Date: Octber 1, f 66 Revised:

11 DWR-Labratry Safety Fill ut as much f the frm as pssible; sme infrmatin may be cmpleted by DWR HR ffice. Submit a cpy f the frm by r fax within 24 hurs. Submit riginal f frm by hand r inter-ffice mail as sn as pssible. Supervisr shuld keep a cpy f the frm (3) WC Release f Infrmatin Frm: As sn as pssible, the injured emplyee must submit a signed and dated riginal f this frm t their immediate supervisr. The frm must als be signed and dated by the supervisr (r a witness). The supervisr shuld then submit the frm t the DWR Human Resurces Office r the DEQ Wrker s Cmpensatin Administratr. (4) Other Frms: There are ther frms that must be cmpleted based n the prfessinal medical care that is needed. These frms are required t dcument leave ptins, mileage, and prescriptin drug reimbursements assciated with a wrker s cmpensatin case. Frms shuld be submitted t the emplyee s supervisr, then t the DWR HR Office. (5) Resurces: The frms mentined abve, as well as prcedures fr Wrker s Cmpensatin, may be fund at the fllwing web-pages: NC Office f State Human Resurces: DWR Safety Web-Page: Accident and Incident Reprting Prcedures All near-miss incidents, accidents, and injuries must be dcumented using the fllwing frms frm the NC Office f State Human Resurces. These frms must be cmpleted as sn as pssible, and submitted t the emplyee s immediate supervisr. The riginal cpies f the cmpleted frms shuld be submitted t the DEQ Safety Office. Cpies may be kept by the supervisr and/r emplyee, if desired. (1) NC Emplyee Incident Reprt Required t be filled ut by the emplyee, and then submitted t their immediate supervisr within 48 hurs f the incident. (2) NC Supervisr Incident Investigatin Reprt Effective Date: Octber 1, f 66 Revised:

12 DWR-Labratry Safety Required t be filled ut by the emplyee s supervisr as part f the investigatin f the incident. The investigatin shuld begin within 24 hurs, if pssible. (3) NC Witness Statement Frm Optinal frm t be cmpleted by anyne wh witnessed the incident, which shuld be submitted t the supervisr r Labratry CHO within 48 hurs f incident. (4) Submit package f the cmpleted frms t the DEQ Safety Office within 72 hurs f incident. (5) Resurces The abve frms may be fund at the NC OSHR website: This web-page als includes an verview f the reprting prcess. 4.5 Accident Infrmatin The fllwing infrmatin, at a minimum, is t be btained by the unit supervisr and Labratry CHO after an accident r injury t assist in dcumenting and investigating the incident: Name f emplyee(s) invlved and cntact infrmatin. Lcatin f incident. Date and Time f incident. What cnditins led t the incident. Descriptin f the incident and any resulting injuries. Lab Unit(s) f invlved emplyee(s). 5 WSS CHEMISTRY LABORATORY SAFETY COMMITTEE Members f the WSS Chemistry Labratry Safety Cmmittee are chsen n a vlunteer basis nly. Each labratry grup/unit will send a nn-managerial staff member t the scheduled meetings. There are n frmal qualificatins, ther than being a WSS Chemistry Labratry emplyee, t serve n the Safety Cmmittee. 5.1 Duties f the Members The duties f the Labratry Safety Cmmittee members are: T assist the WSS Chemistry Labratry Chemical Hygiene Officer in perfrming peridic safety inspectins f the Labratry. A reprt f the inspectins will include any deficient cnditins r practices nted, crrective measures needed, and a time schedule fr implementatin f the crrective measures. The reprt will be frwarded t the WSS Chief, the WSS Chemistry Labratry CHO, and the Department Safety Directr. Effective Date: Octber 1, f 66 Revised:

13 DWR-Labratry Safety T review the circumstances and causes f accidents and reprted near-miss incidents, and t recmmend crrective measures. T bserve and reprt any unsafe practices r cnditins. T reprt and discuss safety suggestins made by fellw emplyees. T recmmend any needed changes in the WSS Chemistry Labratry r unit SOPs. T set an example fr safety at all times. 5.2 Organizatin f the Safety Cmmittee The rganizatin f the WSS Chemistry Labratry Safety Cmmittee is as fllws. 1. Meetings will be held n the first Mnday f every mnth at 10:30 AM in the emplyee break rm. 2. Meetings will last apprximately 30 minutes. 3. Cmmittee members will serve terms f at least 12 mnths. Cntinuity f the Cmmittee will be maintained by replacing n mre than ne third f the members in any given 12-mnth perid. 4. Attendance f members is mandatry. 5. Meeting agendas will be limited t safety-related issues and prblems. 6. The chairpersn will serve a term lasting at least 12 mnths, with all members having equal vice in selecting/electing the new chairpersn. 5.3 Safety Checks The members f the WSS Chemistry Labratry Safety Cmmittee are als respnsible fr cnducting regular safety checks within their labratry grups/units. The safety checks shuld be recrded, and any deficiencies shuld be discussed at the subsequent safety meeting, r with the Labratry CHO. Rutine safety check prcedures are: 1. Mnthly, each f the eyewashes and drench hses in the grup/unit, whether lcated at a lab sink r at a safety shwer, shuld be checked fr prper water flw. 2. Mnthly, the safety shwers in the grup/unit shuld be perated fr several secnds t flush the pipes and t check that water is flwing prperly. A nyln sleeve, attached t PVC plastic handle, is used t direct water frm the shwer int a bucket. 3. Mnthly, the fire extinguishers lcated in the grup/unit shuld be checked. See sectin 14.4 fr mre infrmatin. 4. Quarterly, the fume hds lcated in the grup/unit shuld be evaluated. See sectin fr mre infrmatin. The Safety Cmmittee s chairpersn and vice-chairpersn will be respnsible fr cnducting safety checks fr the eyewashes, safety shwers, and fire extinguishers that are lcated in the hallways and cmmn areas, as well as the labratry s Autmated External Defibrillatr (sectin ). 5.4 Incident Investigatin by the Safety Cmmittee The purpse f accident / incident investigatin is t cllect facts, nt t place blame. The aim f this investigatin is t determine the rt cause f the accident / incident. Accident / Incident investigatin will place emphasis n: Effective Date: Octber 1, f 66 Revised:

14 DWR-Labratry Safety Was the accident / incident caused by an unsafe act r cnditin? Was the emplyee ding anything that was unsafe? If s, what reasn did the emplyee give fr nt acting safely? What steps need t be implemented t prevent similar ccurrences in the future? 6 CHP AND SAFETY TRAINING It is the direct respnsibility f the Managers, Supervisrs, and WSS Chemistry Labratry CHO t ensure that safety training is available t emplyees. It is the emplyee s respnsibility t make maximum use f the training pprtunities prvided t them. 6.1 New Emplyee Orientatin and Training On emplyment with the WSS Chemistry Labratry, new persnnel (permanent, part-time, temprary, interns, etc.) will be riented t the general safety prcedures in the labratry and will be given the pprtunity t read the. This initial rientatin and training shuld be dcumented n frm TRF (see Appendix). Each new emplyee will be required t sign a frm (TRF-003-1; see Appendix) indicating that rientatin infrmatin was made available, that they have reviewed the, and understand the infrmatin cntained in thse dcuments. The emplyee s Unit supervisr will allw adequate time, befre beginning wrk, t read the dcuments and clarify any areas that are nt understd. The abve frms shuld be kept in the WSS Chemistry Labratry training files. New emplyees f the WSS Chemistry Labratry will receive a general safety rientatin, which will, at a minimum, include: Use f chemicals in the Labratry, the hazards assciated with thse chemicals, and apprpriate chemical waste dispsal prcedures. Accident / Incident preventin and reprting prcedures. Labratry fire safety and evacuatin plans. A tur f the Labratry facility. 6.2 Unit Specific Chemical Hygiene Training Emplyees will be apprised f the hazards present in the wrkplace upn initial assignment t the Unit r whenever new chemicals r prcesses are intrduced int the wrk area. Unit supervisrs, r a designated emplyee, will be respnsible fr unit specific chemical hygiene training fr new r newly transferred emplyees, and will schedule a safety rientatin with the Chemical Hygiene Officer. Unit safety training is t be dcumented n frm TRF (see Appendix); the riginal shuld be kept in the WSS Chemistry Labratry training files. At a minimum, emplyees are t be trained in the fllwing areas: The cntents f the and hw it applies t the Unit. The lcatin and general cntents f the Unit Safety Data Sheet (SDS) file. This training can be handled in a hazard class basis fr nrmal chemicals; hwever, specific, particularly hazardus chemicals must be cvered in detail t ensure emplyees are aware f the chemical s hazardus prperties. Effective Date: Octber 1, f 66 Revised:

15 DWR-Labratry Safety The current Permissible Expsure Limits (PEL s) fr expsure t chemicals in the Unit. The detectin f leaks r releases f chemicals in the Unit and specific cleanup prcedures t be used. The persnal prtective equipment required t be used in the Unit. 6.3 Additinal WSS Chemistry Labratry Training Additinal training curses will be made available frm time t time. These curses may be mandatry r ptinal, depending n the tpic. Emplyees are required t attend all mandatry training and are encuraged t take any ptinal training. Optinal training may include such training as First Aid r CPR training. 7 CHP MEDICAL SURVEILLANCE All emplyees in the WSS Chemistry Labratry shuld receive a baseline physical within 3-6 mnths f beginning wrk in the Labratry. Emplyees may als receive a physical peridically during their emplyment with the WSS Chemistry Labratry. These examinatins are prvided at n cst t the emplyee. Duke Occupatinal Medicine perfrms the baseline physical at their Research Triangle clinic. The examining physicians are ccupatinal medicine physicians. A medical histry will be cmpiled, and specific bld tests may be perfrmed. During the baseline and any fllw-up exams, it is particularly imprtant that each emplyee infrm the examining physician f the type f wrk perfrmed in the Labratry and the types f substances t which expsure may ccur. Substances f particular interest are heavy metals, chlinesterase inhibitrs (pesticides), and ptential carcingens. 8 VISITORS TO THE WSS CHEMISTRY LABORATORY Persns nt in the emply f the WSS Chemistry Labratry shuld be cnsidered visitrs t this site. Access t the WSS Chemistry Labratry building is t be regulated as the Sectin des enfrce the FDA and EPA rules regarding chain f custdy fr sme f the samples entering this building. Certain individuals that frequent the premises may, after initial rientatin and hazard training, be allwed t freg the visitr sign in prcess. Determinatin f exclusin frm the nrmal visitr prcess will be dne n a case by case basis fr these persns. All visitrs (excluding Facility Management and DWR persnnel) are t sign the Visitr Lgbk. Visitrs are t be met in the frnt ffice area by the emplyee wh the visitr is requesting t see. Visits int restricted areas f the Labratry building will be at the discretin f the Unit Supervisr, r the Labratry CHO. Prir t entering a restricted area: 1. Visitrs will receive a safety review fr the specific area befre they prceed t that area. 2. Visitrs will be issued apprpriate persnal prtective equipment fr that area 3. Repairmen, maintenance persnnel, r cnstructin emplyees will be instructed in the hazards assciated with areas arund their wrk sites. The Department Safety Directr r the Labratry CHO will review their tasks and issue apprpriate instructins and precautins. 4. Visitr injuries require an Incident Investigatin reprt be cmpleted and frwarded t the Branch Supervisr, the Labratry Safety Cmmittee, the Sectin Chief and the Department Safety Directr. Effective Date: Octber 1, f 66 Revised:

16 DWR-Labratry Safety 9 CHP REVIEW AND UPDATE In cmpliance with the current OSHA regulatins cncerning the Labratry Standard, 29 CFR , and any amendments made t thse regulatins by the Nrth Carlina Department f Labr s Divisin f Occupatinal Safety and Health, the will be reviewed and updated n an ANNUAL basis. The review prcess will include crdinatin with the WSS Chemistry Labratry Safety Cmmittee, Chemical Hygiene Officer, and the Department Safety Office. If substantial changes are made t the Plan during this prcess, all WSS Chemistry Labratry emplyees will receive updated training in the changes made t the Plan. 10 CHP EMERGENCY RESPONSE The ptential fr an emergency t ccur at the WSS Chemistry Labratry is very real. The Labratry has n premises ptentially hazardus chemicals, cmpressed gases, ptentially carcingenic materials, flammable and cmbustible liquids, and ther similar types f materials. Types f emergencies that can ccur include but are nt limited t: Fires Chemical Spills Bilgic Cntaminatin Security Threats The general frm f respnse will be t perfrm either a general r lcal area evacuatin General Evacuatin A general evacuatin requires all persns t exit the labratry building. Rutes and exits are marked by exit signs and n the WSS Chemistry Labratry Evacuatin Map (see Appendix), which shuld be displayed n each unit s main exits. The primary assembly area fr all Labratry persnnel is the NORTH SIDE f the labratry building in the emplyee parking area. In case f inclement weather, the alternate assembly area will be the SOUTH SIDE f the 4401 building (Water Quality). The WSS Chemistry Labratry CHO, r assigned persn, shuld take the visitr lg t the assembly area. The lg shuld be reviewed t determine if there are any visitrs in the lab building. A rll call f emplyees will be made by the supervisry staff t ensure that emplyees frm all lab units, as well as any visitrs, have been evacuated. Emplyees shuld nt return t the labratry building until instructed t d s by the Sectin Chief, WSS Chemistry Labratry CHO, r EMS persnnel. In the event f an evacuatin f the lab building, a supervisr shuld check with emplyees in the Certificatin mdular building t insure that they are aware f the situatin Lcal Evacuatin A lcal evacuatin des nt require exit frm the building. Occupants f the Unit are t leave the Unit and assemble in the hallway utside the Unit, at an apprpriately safe distance. D nt hinder emergency r cleanup peratins. Take the SDS file t the safe distance lcatin if safety permits. Effective Date: Octber 1, f 66 Revised:

17 DWR-Labratry Safety 10.3 Specific Evacuatin Prcedures Fire Activate the nearest pull alarm. Leave the area and ensure that all ther persnnel in the affected area have been evacuated. Call t activate the fire emergency services. Prceed with the general evacuatin plan and assemble at the utside assembly area Gas Leaks In case f a leak f an explsive, flammable, asphyxiating r crrsive gas, prceed with the general evacuatin plan Chemical Spills The nature f the chemical spill will determine the level f evacuatin and emergency respnse. Treat all chemical spills with apprpriate cautin. Determinatin f the apprpriate cleanup r emergency respnse measures will be made frm the evacuatin assembly lcatin. Types f Spills: A small spill is defined as a spill that invlves the equivalent f less than 500 milliliters f a chemical substance and the substance is nt a highly hazardus substance. Fr small spills, perfrm the necessary evacuatin t a lcal evacuatin pint, and refer t the chemical specific Safety Data Sheet (SDS) fr apprpriate cleanup prcedures, persnal prtective equipment t be utilized, and fr any ther safety related precautins. A large spill is defined as a spill f a substance that invlves the equivalent f 500 milliliters r mre and the substance is nt a highly hazardus substance. A large spill may necessitate the evacuatin f the Labratry building r a substantial prtin f the building. Fr spills invlving particularly hazardus substances, such as asphyxiating, explsive, highly reactive, etilgic, r flammable chemicals, prceed with the general evacuatin prcedure Chemical Spill Plan An imprtant part f a safety plan is the review f all pssible spills ahead f time. The Chemical Spill Plan fr the DWR Central Labratry is lcated in Appendix A and prvides guidelines fr assessing the ptential fr a spill and hw t respnd in the event f a spill. Fr each lab unit, the necessary spill cntrl materials shuld be readily at hand and all persnnel shuld be trained in their use. Spill cntrl items shuld include pwders fr neutralizing acids/bases and fr absrbing rganic slvents, absrbent pads and pillws, and waste bags r cntainers Spill-X Absrbent Pwders One f the primary spill respnse items in the labratry is absrbent pwder, which serve t absrb a spilled chemical while als neutralizing the substance. Bttles f Spill-X pwders are available in each lab unit; there are 3 types f pwders based n use fr acids, bases, and rganic slvents. Guidance is prvided in Appendix B Mercury A spill invlving mercury requires a clean-up kit and prcedure that is specific t handling mercury and insuring that mercury vapr is minimized. The primary surces f a ptential mercury spill in the labratry are mercury thermmeters. In the event f a brken mercury thermmeter, the unit supervisr Effective Date: Octber 1, f 66 Revised:

18 DWR-Labratry Safety and the Labratry CHO must be ntified immediately t determine if any mercury has been released and t cnduct clean-up prcedures. Guidance fr using the mercury spill kit (lcated in the stck rm, G082) is in Appendix C. Only persnnel trained in use f this kit shuld attempt t clean up a mercury spill in the labratry Medical Emergencies A medical emergency is defined as a situatin where a persn is injured t an extent greater than first aid measures can accmmdate and the affected persn needs prfessinal medical attentin. Types f medical emergencies may include deep cuts r punctures, thermal r chemical burns, eye injuries, and similar cnditins. If a medical emergency is serius r life-threatening, cntact EMS via telephne by dialing 9-911, and request an ambulance r ther emergency service t respnd. Stay n the telephne until instructed therwise by the EMS peratr. If a medical emergency is determined t nt be serius r life-threatening, and the injured emplyee can be transprted SAFELY and withut any further injury r aggravatin, state autmbiles may be utilized t transprt the emplyee t a lcal hspital r emergency care center (see sectin 4.1) Other Threats 10.6 Drills Other threats may necessitate a general evacuatin r emergency respnse. These evacuatins and respnses may be called by management, ther safety persnnel, r lcal emergency r law enfrcement persnnel. Fllw the instructins given by the management team, r ther EMS persnnel in charge. City Water Outage: In case f a city water utage, the labratry fire/sprinkler system is functinal but eye washes and safety shwers will nt wrk. Any prcedures that may pse a safety hazard requiring pssible use f eye wash r safety shwer shuld cease r nt be perfrmed. Trnad: If a trnad warning is issued fr the area f the WSS Chemistry Labratry building, emplyees will be directed t mve ut f rms with large windws. Emplyees shuld mve int the central hallway, r a rm withut windws (e.g., break rm). D nt return t regular wrk areas until cleared t d s. A weather radi is maintained in the frnt ffice area; the radi has a back-up battery in case pwer is disrupted, and the weather alarm will sund when a trnad r severe weather warning is issued fr Wake Cunty. General evacuatin drills (fire drills) shuld be arranged by the Labratry Chemical Hygiene Officer. A fire drill shuld be scheduled thrugh Facility Management (Department f Administratin), by sending an t the Fire Preventin Officer (currently Jake Eller). Fr a drill, the labratry building fire alarm shuld be activated by persnnel frm Facility Management. General evacuatin drills must be cnducted n an annual basis (at a minimum). Recrd infrmatin fr each drill (date, time t evacuate building, etc.) using the frm in the Frms Attachments sectin. The cmpleted frm shuld be maintained at the labratry, and a cpy frwarded t the Department Safety Office. Effective Date: Octber 1, f 66 Revised:

19 DWR-Labratry Safety 11 PERSONAL PROTECTIVE EQUIPMENT The Divisin prvides certain persnal prtective equipment t emplyees at n cst. This equipment is made available fr persnal prtectin. D nt misuse r abuse the equipment. Misused and abused equipment will be replaced at the emplyee s expense. The WSS Chemistry Labratry maintains a washer and dryer fr laundering f certain articles. These articles, which include lab cats, are t be washed n site and nt taken hme fr laundering Hazard Assessment The Department Safety Office, in cnjunctin with the CHO, the Unit Supervisrs, and emplyees f the lab unit shuld perfrm jb hazard assessments as needed n the basis f a significant change in prcedure r equipment. The reasn fr the jb hazard assessment is t indicate all persnal prtective equipment that will be needed t perfrm the particular jb safely. Training will be given in the apprpriate fitting, use, care, and strage f any persnal prtective equipment that is issued Eye Prtectin Divisin plicies require the use f eye prtectin when there is a reasnable prbability f eye injury. Apprpriate prtective devices shall be wrn by emplyees and visitrs in all areas identified as Eye Hazard areas. There are n exceptins t this plicy. Cntact lenses can trap and retain chemicals and vaprs frm the air and cautin shuld be used when wearing cntact lenses in areas where labratry chemicals are used r stred Types f Eye Prtectin Varius types f eye prtective gear may be used in the WSS Chemistry Labratry. The specific type will be determined during the Jb Hazard Assessment. Varius types and sizes f eye prtectin will be available fr use by emplyees and visitrs. This may range frm simple impact resistant eye wear t splash resistant gggles. Emplyees that nrmally wear prescriptin eye wear and are required t wear safety eye wear while perfrming their jbs will be able t btain safety eye wear thrugh specified suppliers. Any cst f the eyewear abve the authrized cst will be brne by the emplyee Eye Wash Equipment Safety eye wash equipment is lcated thrughut the WSS Chemistry Labratry units. It is the respnsibility f each emplyee t ensure they knw the lcatin f the clsest eye wash statin. If eye washing is necessary, washing is t be perfrmed fr a minimum f 15 MINUTES Face Prtectin Eye wash statins are t be tested by the unit safety cmmittee representative n a mnthly basis t ensure that this equipment is perating prperly. Apprpriate dcumentatin f the testing will be maintained by the Unit Safety Cmmittee Representative and archived by the CHO. A full face visr may be needed when: Handling cncentrated acids r bases Using flammable slvents Heating glassware Effective Date: Octber 1, f 66 Revised:

20 DWR-Labratry Safety Using equipment when cntents are under pressure 11.4 Hand and Ft Prtectin Glves, f the apprpriate type, are t be wrn when handling: Chemicals (At minimum, the Nitrile dispsable type are t be used.) heat surces extremely cld (subzer F) substances sapy glassware that is being washed Dispsable nitrile glves are the standard hand prtectin in the labratry and shuld be wrn when handling chemicals r samples. Nte: Dispsable Nitrile glves may nly prvide shrt- term prtectin frm sme slvents and cncentrated chemicals. See Appendix D fr guidance n types f glves and chemical resistance. Dispsable Nitrile glves may nt be suitable fr hand-washing large labratry glassware (e.g., separatry funnels) since they are thin (and nt puncture-resistant) and shrt in length. Nitrile glves als can becme slick when wet, s cautin shuld be used when handling wet glassware. Latex r latex type glves that cver the frearm and have grip finger tips shuld be used when washing such large labratry glassware t help guard against slippage causing brken glass and pssible injury. Any dermatitis r allergies shuld be reprted t the emplyee s supervisr r the CHO, and must be evaluated by the Department Safety Office. If needed, dispsable glves made frm a different material shuld be rdered. Thermal glves are t be wrn when handling extremely cld r ht bjects r substances. Apprpriate chemical prtective glves are t be wrn in additin t the dispsable glves when handling hazardus chemicals. Specific types f chemicals may require glves made f specific material and greater thickness, especially if expsure t the chemical will be prlnged. Cnsult the SDS and vendr infrmatin t determine the glve material that prvides the maximum amunt f prtectin. As an extra precautin, emplyees shuld wash their hands thrughly and immediately after handling samples r chemicals. Prper clthing and ft cvering are vital t individual prtectin. Shrts, sandals and pen-ted/ heeled shes are nt t be wrn in areas where chemicals are handled and/r samples are prcessed and analyzed Trs Prtectin Lab cats are t be wrn whenever emplyees are actively running tests, setups, r handling chemicals. The lab cats are t be buttned t prtect the wearer s street clthes. The laundry facility is t be used t clean the lab cats (d nt take these cats hme t launder). Chemical resistant aprns shuld be wrn when handling large vlumes f cncentrated acids and bases Respiratry Prtectin The envirnment f the WSS Chemistry Labratry is mnitred n an as needed basis t minimize expsure t airbrne cntaminants. If the envirnmental mnitring indicates that OHSA permissible expsure limits are in danger f being exceeded, then additinal engineering r administrative cntrls will be instituted. In the event that engineering r administrative cntrls are nt adequate t cntrl an Effective Date: Octber 1, f 66 Revised:

21 DWR-Labratry Safety airbrne cntaminant, specific respiratry prtective prcedures will be implemented n a case by case basis. At least tw members f the WSS Chemistry Labratry Safety Cmmittee shuld prcure and be fit-tested fr full r half-face respiratrs. The Department Safety Office will assist in selectin and fit-testing f the respiratrs. The respiratrs shuld be wrn fr transfer f slvent waste t the waste strage drum, and fr cleanup f spills invlving a chemical that presents an inhalatin hazard. Fr mre infrmatin cncerning the use f respiratry prtective equipment, refer t the Divisin f Water Resurces Respiratry Prtectin prgram. 12 FIRST AID 12.1 General While n the jb, any injury that ccurs is t be reprted t the emplyee s immediate supervisr. In the WSS Chemistry Labratry, n persnnel are designated as first aid respnders; emplyees are nt required t administer first aid, but can d s if they have received training. First aid kits are lcated n each end f the labratry s main hallway (near the drinking funtains), and can be used fr minr cuts, scrapes and burns. Mre severe injuries will require attentin frm a medical prvider. If medical attentin is sught, the incident is t be frmally reprted, in writing, t the emplyee s supervisr and t the Department Safety Office. See Sectin 4, Accident / Incident Reprting, fr further infrmatin Chemical Expsure and First Aid If an emplyee is expsed t a chemical, quick respnse is essential. Fr eye cntact (see sectin ) and fr skin cntact, immediately flush the area with water fr a minimum f 15 minutes. Fr serius chemical expsures, emplyees are t be transprted t the clsest medical facility via EMS. A cpy f the SDS fr the chemical shuld t be taken with the emplyee s that the medical prvider will knw the type(s) f chemical(s) invlved. Additinally, the Emergency Ntificatin Infrmatin sheet kept in the frnt ffice shuld be taken with the emplyee t the medical prvider Chemical Cntact t Bdy 1. Quickly remve all cntaminated clthing and begin using a safety shwer r drench hse. Begin flushing the affected bdy area with water as sn as pssible. Be careful t avid spreading the chemical t surrunding skin r int the eyes. 2. Flush the affected bdy area with cld water fr at least 15 minutes. Resume if pain returns. 3. If needed, a safety shwer that is equipped with ht water is lcated in the men s restrm. The injured emplyee may be transferred t this shwer after initial rinsing f the chemical(s) frm the bdy. This shwer als ffers mre privacy t the injured emplyee, as well as supplies (twels, gwn, etc.) lcated in a nearby cabinet. 4. If pssible, wash ff chemicals with a mild detergent and water. D nt use neutralizing chemicals, unguents, creams, ltins, r salves. 5. Get prfessinal medical attentin as sn as pssible. Prvide medical persnnel with the exact chemical name s that prper treatment may be started as sn as pssible. Effective Date: Octber 1, f 66 Revised:

22 DWR-Labratry Safety 12.3 Equipment n Site The WSS Chemistry Labratry has tw first aid kits n site. These kits are lcated in the main east - west crridr, near the water funtains. Additinally, anther kit may be maintained in the Chemical Hygiene Officer s ffice. These kits are t be used fr minr injuries nly. The main crridr kit cntents shuld be checked and re-supplied n a mnthly basis. The mdular building fr the Labratry Certificatin Branch has ne first aid kit and will be checked and re-supplied as needed Autmated External Defibrillatr The WSS Chemistry Labratry has an Autmated External Defibrillatr (AED) that is munted n a wall in the frnt ffice (next t rm G003). The AED is used t administer an electric shck t a fibrillating heart f a victim in rder t bring the heart back t its sinus rhythm. When the AED is remved frm its case and the face plate is pened, autmated vice instructin begins giving step-by-step directins fr use n a victim. One f the steps will indicate that the chest is t be fully expsed by remving all clthing in that area s that the cntact pads can be prperly placed and secured n the skin. The device will then determine if shck is needed. Light emitting dides (LEDs) indicate status f the AED and batteries; the LEDs will flash green every few secnds when the AED is fully functinal. The battery fr the AED shuld be replaced as needed, and will generally last fr 5 t 7 years. The AED paddles shuld be replaced prir t the expiratin date printed n the paddles. The status indicatr light is t be checked n a mnthly basis. A training DVD and user manual are available with the device at the munting statin. Imprtant: The AED is intended as first aid; call immediately fr lcal EMS Fire Suppressin Blankets A fire suppressin blanket is lcated at each end f the main east-west hallway. The blankets are hused in rectangular, range-clred bxes. If needed, an emplyee shuld grasp the rpe handle prtruding frm the bx cver and quickly rtate their bdy clck-wise. Once the blanket is surrunding the bdy, the emplyee shuld drp t the grund and rll their bdy t smther the fire. Cautin: D nt remain standing after wrapping in a blanket; this can cause flames t be funneled upwards twards the face. In additin, nly use a blanket is time permits; therwise, use the standard stp-drp-rll prcedure t smther flames First Aid Training All emplyees in the WSS Chemistry Labratry will have the pprtunity t attend First Aid, CPR and AED training. The training is ptinal and persnnel taking the training will nt be identified as Divisin first aid respnders. The training will be scheduled and prvided by the Divisin Safety Cnsultant. Emplyees btaining this training will be certified in First Aid and CPR practices thrugh the Natinal Safety Cuncil in cnjunctin with the Safety and Health Cuncil f Nrth Carlina. Effective Date: Octber 1, f 66 Revised:

23 DWR-Labratry Safety 13 WORK AREA SAFETY AND HOUSEKEEPING Labratry and ffice wrk areas are t be maintained in a clean and rderly manner, and chemicals and lab equipment are t be prperly stred and labeled. In additin, the fllwing safety and husekeeping rules apply t all wrking areas f the WSS Chemistry Labratry: 1. Fr Persnnel and Fire Safety reasns, cell phne use (including Bluetth and ther handless technlgy) is NOT permitted while perfrming labratry prcedures, especially when wrking with hazardus chemicals (e.g., flammable liquids and crrsives). 2. Fd and cnsumptin f fd items is ONLY permitted in defined areas. Cnsumptin f fd and beverage items is NOT t be permitted where labratry peratins are being perfrmed. 3. Glassware and utensils fr labratry peratins are nt t be used fr fd preparatin r cnsumptin. Labratry clers and chemical strage freezers r refrigeratrs shuld never be used fr fd strage. 4. Glassware is t be rinsed immediately fllwing use t prevent thers frm cming in cntact with chemical residues left in r n the items. 5. Wrk areas are t be cleaned prmptly after use and t be kept free frm bstructin. 6. Chemicals and prepared slutins are t be labeled prperly; at a minimum, the chemical name and cncentratin shuld be visible. 7. Chemical Strage: Each chemical r slutin in the labratry shuld be stred prperly and safely. This includes cnsideratin f any specific strage requirements, cmpatibility with ther chemicals, and ease f accessibility. Cnsult the SDS fr a chemical t verify strage requirements. 8. Chemical Cmpatibility: D nt stre acid and bases, r xidizers and reducers, in the same cabinet due t pssible vilent reactin between the tw. 9. Un-labeled cntainers and expired chemicals are t be dispsed f prperly and in accrdance with apprpriate prcedures. Materials and chemicals n lnger needed are nt t be allwed t accumulate in the labratry. 10. Brken Glassware: All brken glassware, as well as Chrmatgraphy needles and Pasteur pipets, must be placed int waste cntainers designated slely fr brken glassware. Each cntainer shuld be lined with a puncture-resistant bag. When full, the tp f the bag shuld be secured and the bx shuld be transprted t the utside dumpster. 11. Never frce glass tubing int a rubber stpper. 12. Flrs are t be cleaned regularly. Spills shuld be cleaned up immediately. Keep flrs free f slip, trip r fall hazards. 13. Place trash cans and persnal items where they will nt present a trip hazard. 14. Stairways and hallways are nt t be used as strage areas. 15. Access t exits, emergency equipment, and building cntrl devices are nt t be blcked. A 44-inch pathway f egress shuld be maintained thrugh all areas f travel in the labratry. 16. Crds and plugs hanging r prtruding frm lab equipment r fume hds shuld be secured. Extensin crds shuld be cnsidered as temprary nly. Only ne pwer strip shuld be used per electrical utlet (d nt cnnect ne pwer strip t anther pwer strip). 17. The three walk-in clers (G028, G035A, and G068) in the lab building each have a panic buttn lcated t the left f the cler dr (n inside wall). The red-clred buttn can be pushed n t activate an alarm that includes a lud audible signal and a red emergency light (n cntrl panel). The Effective Date: Octber 1, f 66 Revised:

24 DWR-Labratry Safety panic buttn shuld be used in the event f an emplyee being unable t pen the cler dr frm the inside Ergnmics Labratry prcedures r wrk tasks that require repetitive mtins, heavy lifting, bending, r lng perids f standing have the ptential t cause musculskeletal disrders affecting the muscles, nerves and tendns. The neck, arms, and lwer back are bdy areas f particular cncern regarding ergnmics, which basically is a prcess f trying t fit a prcedure r task t the wrker in a way that minimizes any chrnic health issues. If any f the abve issues are a cncern, an emplyee shuld ntify their immediate supervisr r the Labratry CHO. The supervisr and CHO shuld assess the prcedure r task t help determine what ptins are available t minimize discmfrt and risk f chrnic injury. The slutin may invlve training and/r a change in wrk cntrls r equipment. The CHO shuld fllw up t insure that the ergnmic issue has been addressed and that the slutin is effective. 14 LABORATORY FIRE SAFETY A Knx Bx is lcated at the east entrance (clsest t Natinal Guard armry) f the parking lt, near the security gate. This secure bx cntains safety infrmatin (SDS frms, building maps) and keys fr the 4401, 4403, and 4405 buildings. The bx can nly be pened by the City f Raleigh Fire Department, and the infrmatin is fr fire department persnnel in case f an ff-hurs fire at ne f the buildings. The Labratry CHO is respnsible fr infrmatin pertaining t the 4405 Building, as well as wrking with persnnel frm the 4401 and 4403 buildings whenever updates are needed Fire System Cntrls The WSS Chemistry Labratry building at 4405 Reedy Creek Rad is equipped with a fully autmatic, supervised sprinkler system. In the event f fire that begins t engulf a rm f the building, the system will activate t cntrl the spread f fire until the city Fire Department arrives n site. This system is inspected quarterly and is maintained in perable cnditin by Facility Management persnnel. The sprinkler riser cntrl valves fr the system are lcated in the strage area (rm G108A), adjacent t the lading dck. Clear access t these cntrls must be maintained at all times. Fire extinguishers are lcated thrughut the WSS Chemistry Labratry building. The lcatins f all extinguishers are shwn n the Emergency Equipment Lcatr that is psted in all labratry rms. All persnnel are expected t knw the lcatin f the nearest fire extinguisher(s). Fire extinguishers are nly t be used in initial-stage fire cntrl. In additin t the autmatic sprinkler system and fire extinguishers, the building is equipped with a fire cntrl system. In case f fire, the building fire cntrl system will activate the audible alarms. When the audible alarms are heard, emplyees shuld immediately leave the building using the mst cnvenient and safest exit. Emplyees shuld assemble with their respective lab units in the grassy area between the parking lt and Reedy Creek Rad. Supervisrs will take rll call t ensure all persnnel have evacuated the building. Management r EMS persnnel will infrm all emplyees when the building is safe t reenter Types f Extinguishers Fire extinguishers are prvided in each f the labratry rms and in the hallway. All f the fire extinguishers in the WSS Chemistry Labratry building are ABC-Dry Chemical, except fr tw carbn Effective Date: Octber 1, f 66 Revised:

25 DWR-Labratry Safety dixide extinguishers. All fire extinguishers are t nly be used n small fires fr initial stage fire-fighting. The dry chemical-class ABC extinguishers are applicable t the fllwing types f fires: Class A: Class B: Class C: Paper, wd, clth, trash, and plastic materials. Flammable liquids (gasline, il, grease, acetne), flammable gases. Electrical equipment; energized electrical equipment fires. Carbn Dixide extinguishers: There are tw carbn dixide fire extinguishers in the labratry building: ne is lcated in the east end f the main hallway, near rm G059; and the secnd is lcated in the west end f the main hallway, near rm G109. These extinguishers can be used fr Class B and C fires, and are primarily intended fr use n electrical fires n analytical instruments (t prevent pssible damage frm the pwder in a dry chemical extinguisher). Use f a CO 2 extinguisher is very similar t a dry chemical extinguisher, but with ne imprtant difference. When a CO 2 extinguisher is discharged, the large discharge cne and supply tube will becme very cld; extreme care shuld be taken t nt tuch these parts during discharge, s as t prevent injury t the user s hands r fingers Other Fire Safety Equipment * Fire alarm pulls are lcated at each f the majr building exits. * Fire Blankets are lcated at each end f main hallway (see sectin ). * Drench Hses / Shwers are lcated in the hallways and lab rms Fire Cntrl System Inspectin and Training 1. The autmatic sprinkler system, fire alarms, and fire alarm pull statins are inspected and tested n a quarterly basis by Facility Management persnnel. Any prblems assciated with these systems are t be reprted t Facility Management. 2. Each fire extinguisher in the labratry building is t be visually checked n a mnthly basis. Each extinguisher shuld be checked fr the fllwing: The safety pin shuld be secure in the handle f the fire extinguisher. The safety pin prevents a fire extinguisher frm being accidentally activated. The needle f the pressure gauge shuld be in the green area, which indicates that the extinguisher is at the prper pressure. If the needle is utside f the green area, ntify the Safety Cmmittee chairpersn r the Chemical Hygiene Officer, wh shuld then cntact the Divisin Fire Safety Officer. An extinguisher that is nt at the prper pressure shuld be replaced as sn as pssible. Check the extinguisher s hse and nzzle. The hse shuld nt have any cracks r signs f wear, and the nzzle shuld be free f debris r any bstructins. The fire extinguisher shuld be easily accessible. If anything is blcking the extinguisher (e.g., bxes r lab carts), the item(s) shuld be mved. 3. If all f the abve items are acceptable, the inspectin attached t the extinguisher shuld be initialed t indicate that the extinguisher has been checked. Effective Date: Octber 1, f 66 Revised:

26 DWR-Labratry Safety 4. Fire extinguishers lcated in the lab units shuld be checked by the unit safety representative. Fire extinguishers in the hallways and ther areas shuld be checked by the safety cmmittee chairpersn and vice chairpersn. 5. An annual maintenance check f all extinguishers is t be perfrmed and dcumented by Department f Administratin (Fire Safety Office). Hydrstatic testing f the extinguishers is t be perfrmed fr all extinguishers in the WSS Chemistry Labratry every twelve years. 6. All persnnel will be instructed in the basic fire hazards assciated with nrmal WSS Chemistry Labratry peratins during their initial assignment training and n an annual basis thereafter Fire Preventin Prcedures Each emplyee needs t have an understanding f the ptential fire hazards with a particular prcedure r fr daily activities f a nn-analytical nature. This invlves an understanding f reagents and equipment, their nature and functin. Fire preventin infrmatin is cntained in the material safety data sheets fr the chemical reagents and the peratr s manuals fr instrumentatin and equipment. Assume that a fire will take place. Be aware f the prbability f fire and plan accrdingly. Labratry fires are generally lcalized and small; hwever, there is a ptential fr a fire t spread ut f cntrl. The ptential fr damage and lss f life must be stressed in rder t insure the effectiveness f fire preventin guidelines. The fllwing represent the minimum perating prcedures fr fire preventin. 1. Smking is prhibited inside the labratry building and inside the gas cylinder cage. 2. Ntify the unit supervisr r WSS Chemistry Labratry CHO immediately f ptentially dangerus situatins. 3. D nt use an pen flame t heat a flammable liquid r t carry ut a distillatin under reduced pressure. 4. Use an pen flame nly when necessary. Befre lighting a flame, remve flammable materials frm the immediate area. 5. Use fume hds during transfer r analysis f flammable slvent(s) t prevent the frmatin f flammable mixtures. 6. Stre flammable materials prperly. * Cntainers must be prperly labeled. * Flammable liquids are t be stred in NFPA-apprved strage cabinets. Cabinets shuld be ventilated if pssible. * Eliminate pssible cntact f flammable liquids r gases with strng xidizing agents, such as chrmic acid, permanganates, chlrates, perchlrates, and perxides. * Exclude ignitin surces frm flammable strage areas. 7. When dispensing and handling flammable substances, ensure that the area is free f ptential ignitin surces (including cell phnes). 8. Use grunding straps with the 55-galln slvent waste drums (lcated in the slvent rm, G102) t eliminate sparks created by static electricity. 9. Befre leaving a lab area at the end f a wrk day, be sure t turn ff all ht plates, pen burners, and ther devices with heat elements. Effective Date: Octber 1, f 66 Revised:

27 DWR-Labratry Safety 10. Place waste material that is impregnated with flammable materials in an apprpriate waste strage cntainer. 11. Smke detectrs are lcated ver every labratry dr. Unit safety representatives need t check each detectr n a mnthly basis by pressing the test buttn, which activates the audible alarm if batteries are still functining prperly. Batteries shuld be replaced n an annual basis, r if the alarm des nt sund during testing. 15 COMPRESSED GASES The WSS Chemistry Labratry uses a variety f cmpressed gases in the nrmal curse f peratins. Cmpressed gases used are Air, Oxygen, Nitrgen, Hydrgen, Helium, Zer Air, and Argn. Cmpressed gas cylinders that are nt in use, with the exceptin f Argn, are stred in the cmpressed gas pen. This utside strage area is lcated adjacent t the lading dck area f the building. Cmpressed gas cylinders are t be treated with cautin. Althugh the cylinders may nt be very large, the cylinders d hld a large vlume f gas. If gas is allwed t escape int the wrking envirnment, the gas will displace the breathing air. Depending n the size f the individual wrk area, the displacement may be enugh t lwer the xygen cntent f the wrk envirnment t the pint f asphyxia r t create an explsive atmsphere. Therefre, the rules belw are t be fllwed when handling cmpressed gas cylinders. The bulk liquid argn tank is mnitred at a remte facility using a dedicated telephne line. If an emergency situatin r a ptential emergency situatin is suspected, call (Arc3 Gases) and reprt the prblem t the Lead Chemist f the Metals unit Mving Cmpressed Gas Cylinders 1. A cylinder truck is t be used when mving cylinders larger than frty punds. Use the chain prvided n the truck t secure the cylinder fr transprt. The truck shuld have 2 supprting wheels in additin t the larger, main wheels. This allws a gas cylinder t be transprted with all f the cylinder s weight supprted by the truck. 2. When exchanging cylinders, ne persn can perfrm the task. Tw r mre peple can be used if ne is uncmfrtable with the lad. 3. Handle cylinders f cmpressed gases as high-energy surces and therefre as ptential explsives. 4. D nt mve a cylinder with the regulating r pressure reducing valves in place. The prtective cap must be secured in place befre a cylinder is mved String Cylinders in the Wrk Area 1. Cylinders are t be stred in the upright psitin, and secured with a strap t a substantial munting bracket s as t prevent tip ver f the cylinder. 2. Use the apprpriate regulatr fr a gas cylinder each cmpressed gas requires a specific regulatr. When attaching a regulatr t a gas cylinder, d nt ver-tighten the nut. Over-tightening the nut may result in the need t use excessive frce when the gas cylinder needs t be changed. 3. After cnnecting a regulatr t a cmpressed gas cylinder, use a leak detectr slutin t verify that the cnnectin is cmpletely sealed and nt allwing any gas t leak. If bubbles are bserved after applying leak detectr slutin, clse the cntrl valve t the cylinder and re-cnnect the regulatr. 4. Use a trap r check valve t prevent back-siphning f chemicals int a cylinder. Effective Date: Octber 1, f 66 Revised:

28 DWR-Labratry Safety 5. Wear safety glasses when handling a cmpressed gas cylinder. 6. D nt expse cylinders t temperatures higher than abut 50 C. Sme rupture devices n cylinders will release at abut 65 C. 7. D nt place cylinders where they may cme in cntact with an electrical unit. Avid areas that are damp r subject t crrsive materials. 8. D nt use a cylinder that cannt be identified psitively. 9. D nt lubricate, mdify, frce, r tamper with a cylinder valve. 10. Under n cnditin shuld high-pressure gases be directed at a persn. 11. A cmpressed gas, including cmpressed air, is nt be used t blw away dust r dirt. 12. Rapid release f a cmpressed gas will cause an unsecured gas hse t whip dangerusly which may als build up a static charge that culd ignite a cmbustible gas. 13. D nt extinguish a flame invlving a highly cmbustible gas until the surce f the gas has been shut ff (if safe t d s). 14. When nt in use, clse cylinder and bench valves. The main cylinder valve is t be tightly clsed, but any needle valves n the regulatr r lines need nly be finger tight t avid ruining the valve and/r valve stem. 15. Never bleed a cylinder cmpletely empty. Leave apprximately 300 psi in the cylinder t ensure the interir f the cylinder is nt cntaminated. 16. Befre lsening a regulatr, check t see if the gas is flammable and if the fitting is reverse threaded. A ntch n the nut f the regulatr indicates reverse threading. Failure t recgnize a reverse threading may result in ver-tightening the nut. 17. When the cylinder is empty, remve the regulatr and mark the cylinder as EMPTY. Separate empty and full cylinders. 18. Fuel gases are t be stred separately frm nn-fuel gases. 19. Oil r grease n the high-pressure side f an xidizing gas (xygen, chlrine, etc.) can lead t an explsin String Cylinders in the Cylinder Pen The cmpressed gas cylinder pen is lcated n the West end f the building, and is adjacent t the lading dck area. This is the main strage area fr the cmpressed gases (new and used cylinders) fr the labratry. 1. Flammable gas cylinders are t be stred n less than 20 feet frm cylinders cntaining xidizers. 2. Empty cylinders are t be marked as EMPTY, and segregated frm FULL cylinders. 3. All cylinders are t be stred with the prtective cap in place, and secured against tip ver. Chains at each cylinder gruping are prvided fr this purpse. 4. The gate t the cylinder pen shuld be kept lcked at all times. Effective Date: Octber 1, f 66 Revised:

29 DWR-Labratry Safety 16 ELECTRICAL & MECHANICAL HAZARDS 16.1 Electrical Hazards 1. DO NOT TOUCH a persn in cntact with a live electrical circuit. DISCONNECT THE POWER FIRST r further serius injury may ccur. 2. All labratry persnnel shuld knw the lcatin f the emergency circuit breakers and hw t cut ff all electrical service t the labratry in case f fire r accident. Each Labratry rm is equipped with an emergency electrical breaker (red buttn) which will discnnect ALL electricity t that rm. This wall switch is used nly fr emergency situatins in rder t extend reliability. 3. Plug equipment int utlets designed t carry the ampere rating f the equipment. All electrical utlets are t be grunded. Electrical utlets in wet areas r within six linear feet f a water surce shuld have grund fault circuit interruptin (GFCI) prtectin. 4. If an electrical crd is wrn, frayed r damaged, repair immediately r mark the crd r equipment as nt t be used. 5. Beware f the explsin hazard f ht plates and flammable liquids under the hds. Maintain a clear area arund cling fans and vents. 6. N flammable gases shuld be stred near high vltage (r ht) equipment. Exceptins include hydrgen tanks near gas chrmatgraphy and acetylene near Inductively Cupled Plasma and Atmic Absrptin Spectrmeter instruments. Label all high vltage equipment. 7. Keep electrical crds free and nt kinked. Turn ff unnecessary electrical devices during weekends and hlidays. 8. Cmputers are t be cnnected t surge prtectin devices. 9. Avid verlading electrical utlets with multiple plugs fr related equipment. 10. Cmbustible items are t be kept away frm heat surces. 11. Refrigeratrs cnstitute a unique hazard because explsins may ccur when they are used fr strage f vlatile r unstable chemicals. Dmestic (huse-hld type) refrigeratrs shall nt be used fr chemical strage unless they are mdified by eliminating pen electrical cntacts and by having the dr clsures replaced with magnetic dr clsures Mechanical Hazards 1. Only trained and designated persnnel are t perfrm maintenance activities n labratry equipment r testing devices. Building-related equipment maintenance, such as heating, ventilatin, air cnditining, plumbing, and electrical, shuld nly be perfrmed by Facilities Maintenance persnnel. 2. Guards, guarding devices, r interlcks are nt t be defeated r remved frm equipment. 3. When perfrming maintenance n guarded r interlcked equipment, prper lck ut prcedure is t be used. All pwer surces (electrical, mechanical, and pneumatic) are t be disabled by any acceptable methd f surce pwer cntrl. The persn perfrming the maintenance shuld maintain cntrl f the lckut devices. Labeling indicating the name f the persn and the lcatin f the maintenance is t be affixed t the mst visible area f the surce pwer 4. Persnnel perfrming maintenance prcedures n equipment are t wear apprpriate persnal prtective equipment. 5. Cmpressed air is nt t be used fr cleaning purpses unless the air pressure has been reduced t less than 30 psi. Effective Date: Octber 1, f 66 Revised:

30 DWR-Labratry Safety 17 VACUUM The term, "vacuum refers t the cnditin f an enclsed space that is devid f air, gases, r ther material cntent. In the WSS Chemistry Labratries, vacuum that is used is mre apprpriately called a partial vacuum because the entire area under reduced pressure is nt ttally devid f gas particles. Vacuum used in the WSS Chemistry Labratry is generally in the range f five (5) t ten (10) inches f mercury (vacuum) Safety Cnsideratins A vacuum apparatus prbably presents fewer accidental hazards than almst any ther kind f lab apparatus. Hwever, these hazards are by n means entirely negligible. The vacuum hazards mst likely t ccur in the labratry are: 1. Implsin This type f hazard is mst imprtant with glass apparatus, and is ever-present when large glass bulbs (ver ne liter in size) r flat bttm vessels (f any size) are evacuated. The frce f atmspheric pressure makes dangerus missiles f glass fragments frm implding vessels. Only use vessels that have been designed fr vacuum. Reduce the pssibility f flying glass by placing strips f plastic electrician's tape n all large glass evacuatin vessels. 2. Explsin When a vacuum system liquefies significant quantities f a gas, r cndensate is taken up by an absrbent at a lw temperature, an explsin can result when the system warms up if adequate vents r safety valves have nt been prvided. An explsin f a different kind can take place if an il diffusin pump (particularly a glass ne) is vented t air while ht Vacuum Desiccatrs The peratinal manual is t be fllwed while using any f the Divisin s vacuum desiccatrs. Each peratr is t make sure he r she is knwledgeable f all cntrls and safety devices befre perating a vacuum desiccatr. Additinal precautins are listed belw: 1. Only chemicals being dehydrated shuld be placed in a desiccatr. 2. When pening a desiccatr that is under vacuum, make sure that the atmspheric pressure has been restred. Refer t the manufacturer s instructin manual fr the crrect prcedure t use when a lid is frzen Water Aspiratrs Aspiratrs fr reduced pressure are used mainly fr filtratin purpses. Only equipment that is apprved fr this purpse shuld be used. These recmmendatins als apply t rtary evapratin peratins where water aspiratrs are being used fr vacuum. 1. Never apply a vacuum t a flat-bttmed flask unless it is very small r it is a heavy-walled filter flask designed fr filtratin. 2. Place a trap and a check valve between the aspiratr and the apparatus s that water cannt be sucked back int the system if the water pressure shuld fail unexpectedly. Effective Date: Octber 1, f 66 Revised:

31 DWR-Labratry Safety 17.4 Vacuum Drying Ovens Labratry persnnel are t fllw the peratr s manual while using vacuum vens. Each peratr is t make sure he r she is knwledgeable f the perating cntrls and safety devices befre perating a vacuum ven. Additinal precautins are listed belw: 1. Always keep drs clsed and lcked whether in use r nt. 2. Turn ff vacuum vens when nt in use. 3. Temperatures shuld be allwed t equilibrate fr tw hurs befre use and mnitred peridically during use. 4. D nt put vlatile chemicals in the ven chamber as this may cause fire r explsin. 18 AUTOCLAVES The WSS Chemistry Labratry has three autclaves fr use by certain lab units. Labratry persnnel are t fllw the manufacturer s perating manual while using these autclaves. Each peratr is t make sure he r she is knwledgeable f all perating cntrls and safety devices befre perating an autclave. Additinal precautins are listed belw: 1. Use prper sterilizer lading prcedures when placing materials in sterilizer chamber. All slid cntainers r instruments must be placed s that water r air will nt be trapped in them. 2. Determine crrect sterilizatin time by referring t minimum sterilizatin time chart r SOP. Sterilizatin will nt be accmplished in less than fifteen minutes expsure time. 3. Opening autclave dr fllwing sterilizatin: When the chamber pressure reading fr an autclave is zer, and the temperature reading is < 50 degrees Celsius, the dr fr the autclave may be pened. Be aware that ht steam may escape the autclave chamber as the dr is pened. 4. All maintenance and repairs f the cntrls and safety devices fr an autclave must nly perfrmed by certified technicians. If an autclave is nt functining prperly, cntact the unit supervisr and schedule a maintenance/repair visit frm a lcal autclave vendr. 5. Prir t perfrming any cleaning r maintenance prcedures, the autclave shuld be at rm temperature. 6. Prtectin: Always wear heat-prtective glves, safety glasses and lab cat when remving a prcessed lad. Prtective glves and aprn shuld als be wrn when relading sterilizer fllwing previus peratin. 7. When sterilizing liquids, t prevent persnal injury r prperty damage resulting frm bursting bttles and ht fluid, set the autclave t LIQUIDS cycle (slw exhaust) and nly use cntainers with vented clsures (d nt use rubber stppers). When using bttles with screw-n caps, always make sure that the caps have been lsened frm the bttle prir t placing the bttles in an autclave. D nt use rdinary glass bttles r any cntainer nt designed fr sterilizatin. 8. Avid sudden full pening f the autclave dr at the end f a sterilizatin cycle. Carefully pen the autclave dr t ne r tw inches, and wait at least ten minutes befre unlading sterilizer. 9. D nt allw ht bttles t be jlted. This can cause ht bttle explsins! D nt mve bttles if any biling r bubbling is present. Allw bttles t cl t tuch befre attempting t mve them frm sterilizer shelf t strage area. Avid steam by standing t the side when pening the dr t the autclave. Effective Date: Octber 1, f 66 Revised:

32 DWR-Labratry Safety 10. A steam supply malfunctin may cause the sterilizer chamber t fill with scalding water. D nt pen chamber dr if the unit fails t cmplete an autmatic cycle r if water leaks past the dr gasket upn unlcking the dr. 11. Sterilizing chamber shuld be drained at the end f each wrk day and left pen t dry. The chamber shuld be cleaned peridically with water and a light detergent, using a nn-abrasive spnge r clth. 19 LABORATORY VENTILATION 19.1 General Building Ventilatin The ventilatin system in the WSS Chemistry Labratry building cnsists f a single pass-thrugh airflw pattern, augmented by chemical fume hds and canpy hds in the varius labratry units. Each part f the system is crucial fr cntrl f air brne cntaminants that may be present in the Labratry building. The single pass-thrugh general exhaust system is designed such that the air entering the building is nt recirculated thrugh the building, but instead is exhausted. Because f this pass-thrugh characteristic, the air changes in the building apprximately 12 times per hur. Additinally, the system is designed t have air flw frm the least hazardus t mst hazardus areas f the building then exhaust, which prevents cntaminatin f lw hazard areas with cntaminants frm the higher hazard areas Chemical Fume Hds General Chemical fume hds are the primary cntainment devices in the labratry t cntrl airbrne cntaminants and fumes generated during experimental prcedures. Chemical fume hds prvide prtectin t persnnel by means f directinal airflw. Air flws frm the labratry area int the hd thrugh the face pening. The exhaust frm the individual fume hds is cmbined and discharged frm the exhaust stack lcated n tp f the building s penthuse (nte this exhaust is separate frm the building s general exhaust system). The fume hds are cnnected t tw exhaust mtrs, with ne active and ne as backup. Emplyees are t use a chemical fume hd when: * Prcedures invlve the use f vlatile chemicals, as well as chemicals exhibiting strng drs. * Chemical prcess results in generatin f txic vaprs r aersls. * There is a need fr additinal physical prtectin against splash, spray, fire, r explsin. * Prcedures invlve the use f a particularly hazardus substance Chemical Fume Hd Use 1. Instructin in the prper use f a fume hd shuld be prvided t all lab emplyees during initial safety rientatin and training. 2. Each fume hd has been certified fr use with the hd sash at a specified height. At this height (marked by a red-clred label), full cntainment f fumes is certified. Abve the maximum certified height, full cntainment is nt guaranteed. Nte that the certified height has t be determined by using ASHRAE prcedures, which invlves air flw measurement, visual smke test, and trace gas measurements. This testing shuld be cnducted by a private fume hd vendr. Effective Date: Octber 1, f 66 Revised:

33 DWR-Labratry Safety 3. When cnducting a prcedure inside a fume hd, equipment and glassware shuld be at least 6 inches frm the frnt f fume hd. An emplyee s head and upper bdy shuld remain utside f the plane f the fume hd pening. Try t minimize mvement near the hd; mvement can cause cnsiderable crss drafts. 4. Elevate equipment and cntaminants ff the surface f the hd (if safe t d s) at least ½ f an inch. If equipment r bttles must be kept inside a fume hd, try t psitin alng the sidewalls; avid blcking the bttm slt f the rear baffle; and avid restricting clsure f the hd sash. 5. The hd sash may be fully pened during set-up and cleaning prcedures that d nt invlve hazardus chemicals. When a fume hd is nt in use, fully clse the hd sash. 6. During a prcedure (e.g., digestin, extractin) r handling f chemicals, the hd sash shuld be psitined at r belw the maximum certified height. Lab drs adjacent t the fume hd shuld be clsed. 7. Each fume hd has a cntrl bx n ne side f the hd pening. There are tw imprtant indicatr lights n the cntrl: Standard Operatin is indicated by a slid green light; Flw Alarm is a flashing red light. In additin, an audible alarm will als sund when the Flw Alarm is activated. The air flw sensr that is cnnected t the cntrl bx is in the Phenix valve (lcated abve each fume hd, this valve cntrls air flw t the hd). 8. If the Flw Alarm is activated fr a fume hd, discntinue any wrk at the hd and clse the hd sash. Ntify the CHO r unit supervisr. If it is determined that air flw at face level is acceptable, then wrk may be cntinued. The cntrl bx shuld be serviced by a private vendr. 9. If the hd mtr shuts dwn r gears dwn, the Flw Alarm fr all fume hds in the lab will activate. This may ccur during a pwer utage r if the fire alarm is activated. In these cases, discntinue wrk in the hd and clse the hd sash. 10. In an emergency, pressing the Emergency Exhaust buttn n the cntrl bx will fully pen the Phenix Valve t allw fr maximum air flw. 11. Maintenance f the labratry fume hds, cntrl bxes and mtr shuld nly be cnducted by Facilities Maintenance persnnel, a fume hd vendr, r a certified repair service. Labratry emplyees are nt authrized t perfrm maintenance n the peratinal cntrls f any chemical fume hd. 12. Fume hds are nt t be used fr permanent strage f chemicals. 13. Hd wrk areas are t be clear f unnecessary equipment and materials that can disrupt airflw and blck vents/baffles. 14. Lab prcedures are t be planned s that, as much as pssible, the materials needed fr a prcedure are present in the hd t eliminate disruptin f airflw caused by carrying equipment in and ut f the hd during a prcedure. 15. Apparatus in the hds shuld be fitted with cndensers, traps, r scrubbers t cntain r cllect slvent r txic vaprs. 16. When a prcedure in a hd is cmpleted, the hd is t be cleaned with an apprpriate cleaner, and the hd sash is t be clsed (hd sash windws shuld als be clsed). 17. Reprt any prblems with a fume hd t the unit supervisr r WSS Chemistry Labratry CHO. Effective Date: Octber 1, f 66 Revised:

34 DWR-Labratry Safety Types f Fume Hds in Labratry Building There are tw makes f fume hds in the labratry: 1. Hamiltn fume hds: These are the lder make f fume hd; all f these fume hds were retr-fitted in 2012 with a duble baffle and imprved air fils fr the hd sash and sash base. Hamiltn hds in Wet Chemistry (G066, except fr Chlrphyll and TDS) and Organics Extractins (G043) have been certified fr use with the hd sash psitined at 24 inches vertical height. All ther Hamiltn hds are certified fr use with hd sash at mid-height (~15 inches vertical height). 2. TFI fume hds: These are the newer make f fume hd, with all plastic parts. These fume hds are certified fr use with the hd sash fully pen. Each make f fume hd als has three mdels, which differ in the width f the hd sash pening. The small mdels have a sash pening width f 38 inches; medium mdels are 62 inches wide; and large mdels are 86 inches wide. Nte: The CHP Appendix includes a table summarizing infrmatin fr each Fume Hd Make and Mdel (with rm lcatins in the lab), with dimensins f the hd sash pening and recmmendatins fr hrizntal psitins t use fr air flw measurements Fume Hd Evaluatin 1. The primary measurement used fr evaluatin f each labratry fume hd is face-level air velcity, which is a measure f air flw at the face plane f a hd. Air flw is measured in feet per minute (fpm) using a velmeter. Fume hd evaluatins shuld be cnducted every 3 mnths by the each lab unit s Safety Cmmittee representative (r ther assigned emplyee). 2. Results fr an air-velcity evaluatin shuld be recrded n a Fume Hd Evaluatin Wrksheet (see Appendix). Cmpleted frms shuld be kept n file in the lab unit, r with the WSS Chemistry Labratry CHO. These frms and any ther written recrds fr fume hd evaluatins must be maintained by the lab unit r WSS Chemistry Labratry CHO fr a minimum f 3 years. The mst current evaluatin recrds shuld be stred in the labratry unit, such that they are readily accessible t lab persnnel. 3. The evaluatin prcess fr a fume hd shuld include: * Measuring and recrding air flw at the face pening f the fume hd * Mapping f the airflw at varius psitins acrss the face pening f the hd * Use f individual measurements t determine if air flw is being reduced by equipment inside f hd; use f average f air flw measurements t determine that face-level air velcity is acceptable (verifying that maximum sash height still guarantees fume cntainment) * General cnditin inspectin 4. Psitins fr Air Flw Measurements: Effective Date: Octber 1, f 66 Revised:

35 DWR-Labratry Safety Fr air flw measurements, vertical and hrizntal psitins must be established t frm a grid acrss the hd sash pening. These psitins can be marked visually n the fume hd t assist in psitining the velmeter. Vertical Psitins: 3 (1) Belw hd sash; (2) Mid-pint between hd sash and base f hd; (3) Base f hd. Hrizntal Psitins: 6 Maximum f 6; start at r near the left side f sash pening, then evenly space ther psitins acrss the width f the sash pening. 5. Set-Up fr Air Flw Measurements: Psitin the hd sash at the Certified Maximum Height. Clse drs t the labratry rm that are near the fume hd. If lab equipment is rutinely maintained inside the fume hd, leave in place. 6. Velmeter: Set the rifice dial (n the left side f the velmeter) t Lw; this setting crrespnds t the bttm scale n the readut display (range f 0 t 200 feet per minute). T measure air flw, hld the velmeter at a designated psitin. The velmeter shuld be in the hd sash plane, in line with the hd sash base. The rifice dial shuld face ut twards the lab area (the screen intake shuld face in twards the hd interir). Hld the velmeter steady and allw the red needle t stabilize, and bserve the reading (t nearest 5 fpm). Recrd the value n the evaluatin wrksheet. Repeat fr all measurement psitins. 7. Acceptance Limits fr Fume Hd Air Flw: The general acceptance limits fr face-level air velcity are 80 t 120 feet per minute. Effective Date: Octber 1, f 66 Revised:

36 DWR-Labratry Safety In Wet Chemistry (G066) and Organics Extractins (G043), the Hamiltn fume hds that have been certified fr use with the hd sash at 24 inches have acceptance limits f 60 t 100 feet per minute. If the average face-level air velcity measurements fr a fume hd are utside f acceptance limits, ntify the unit supervisr r the WSS Chemistry Labratry CHO. 8. In additin t the quarterly in-huse evaluatins, the labratry fume hds shuld als tested by a certified fume hd vendr at a frequency f 1 t 2 years, r if in-huse testing indicates pssible prblems with fume cntainment. This testing shuld include face-level air flw measurements and mapping, smke test, and general inspectin f fume hd sash and interir. Nte that full ASHRAE testing is nly required if there is a significant change t a fume hd, r if the certified maximum sash height needs t be cnfirmed r adjusted. 20 BIOLOGICAL MATERIALS HAZARDS WSS Chemistry Labratry emplyees may be expsed t bilgical material hazards. These hazards include bacterilgical cntaminatin f water samples, wildlife tissue samples, bacterial cultures, and cntaminants in industrial wastewater samples. Prper handling f ptentially cntaminated samples can be achieved as fllws: 1. Use apprpriate persnal prtective equipment t prtect against splash, spray, spill, r physical cntact. 2. Equipment and cntainers used t cntain, r used in test prcedures invlving bilgical material hazards, are t be autclaved prir t washing r reuse. 3. Petri dishes used fr plating ut clny cultures are t be placed in an autclavable bihazard bag and sterilized prir t dispsal. 4. Strage f bilgical cntaminants and the areas fr bilgical cntaminatin strage are t be clearly marked and labeled as having ptential bilgical cntaminatin. 5. Spills cntaining bilgical cntaminants are t be cleaned up immediately, with cntaminants placed in an apprpriate bilgical material hazard bag. An apprpriate sterilant is t be used n surfaces cntacted by spilled material. 6. Wrk surfaces where bilgical materials have been handled are t be cleaned at the end f each wrk shift using an apprpriate sterilant (e.g. 70% isprpanl). 7. Emplyees shuld regularly wash hands with warm water and sap. 8. Never pipette bacterial samples by muth always use pipette bulbs. 21 RADIOACTIVE MATERIALS HAZARDS The Pesticide Analytical Grup f the WSS Chemistry Labratry, in rm G047, has gas chrmatgraphs equipped with Electrn Capture Detectrs (ECDs) that cntain a beta particle emitter (lw energy electrns) made f Ni63 metal fil. The fil munted inside f the husing f the detectr is used fr detecting the presence f halgen-cntaining cmpunds such as fund in halgenated pesticides. The detectr is a sealed unit that can be cleaned and serviced nly by a technician r manufacturer licensed t handle radiactive materials. Since it is cnsidered a radiactive surce, specific rules must be fllwed when using, string, and handling these detectrs: 1. A general license fr pssessin and strage f radiactive material must be maintained by the WSS Chemistry Labratry. The license is issued by the Radiatin Prtectin Sectin f NCDEQ. The Effective Date: Octber 1, f 66 Revised:

37 DWR-Labratry Safety labratry currently hlds a license (number G) issued n March 16, The labratry Safety fficer is the cntact persn fr the lab. 2. Only authrized and trained persnnel are t use the Electrn Capture Detectr equipment. Users are t ensure all perating prcedures are fllwed t minimize expsure t this radiactive surce. A transprt cntainer is t be used when taking the surce ut f the lab fr cleaning. 3. Hand prtectin in the frm f glves is t be used when handling an ECD. Even if wearing glves, a thrugh washing f hands is t be perfrmed after handling an ECD. 4. Labratry emplyees shuld nt pen r therwise tamper with an ECD. 5. As per State f Nrth Carlina regulatins (15A NCAC ), all ECD s in use are t be tested fr leakage f radiactive material ( wipe tested ) every six (6) mnths. Testing is nt required fr ECD s being held in strage, except fr when they are scheduled fr transprt/dispsal. Leakage tests must be perfrmed by a persn hlding a specific license authrizing prvisin f this service. Test recrds fr an ECD shuld be maintained by the labratry safety fficer until the ECD is remved frm the lab. 6. If an ECD is replaced r will n lnger be in use, the instrument manufacturer shuld be cntacted t have the ECD remved frm the lab. An ECD can be remved frm an instrument by labratry persnnel, but shuld nly be remved frm the labratry by the instrument manufacturer (r shipped t the manufacturer). 7. If an ECD is t be shipped t the instrument manufacturer, it must be transferred by licensed curier (e.g., FedEx). A reprt shuld be submitted t the Radiatin Prtectin Sectin with the fllwing infrmatin: identificatin f the device by manufacturer s name, mdel number, and serial number; name, address, and specific license number f persn receiving the ECD; and the scheduled date f transfer. 8. ECD s that are n lnger in use (nt attached t an instrument) shuld nly be stred in the lab temprarily (maximum f 3 mnths). Strage f ECD s is t be in Rm G047, the Pesticide Instrument Rm. Strage is t be in a lcked strage area that is slely fr radiactive material. The strage area is t be identified using the standard radiactive surce marking signs. 22 PARTICULARLY HAZARDOUS SUBSTANCES In the nrmal curse f wrk in the WSS Chemistry Labratry, emplyees may be expsed t chemicals that are designated as Particularly Hazardus Substances (PHS). A chemical identified as a PHS may r may nt have severe acute effects, but with repeated expsure may pse a significant threat t human health. Expsure t a PHS may ccur during prcedures such as extractin r analysis f envirnmental samples, handling f chemical standards, r frm the handling f envirnmental samples received frm the field ffices. Ptential expsure rutes include accidental ingestin, absrptin thrugh skin, eye cntact, and inhalatin. A Particularly Hazardus Substance is a chemical r substance that falls under ne r mre f the fllwing categries: (1) Carcingen r suspect carcingen: a chemical that may cause cancer after repeated expsures. Determined by ne r mre f the fllwing: * OSHA publicatins, especially 29CFR1910, subpart Z, which is a list f carcingens r ptential carcingens. * Natinal Txiclgy Prgram s Annual Reprt n Carcingens, 12 th Editin, which identifies substances that are knwn r reasnably anticipated t be human carcingens. Effective Date: Octber 1, f 66 Revised:

38 DWR-Labratry Safety * IARC (Internatinal Agency fr Research n Cancer) mngraphs, specifically chemicals that are listed in either Grup 1 (carcingenic t humans) r Grups 2A r 2B (prbably r pssibly carcingenic t humans). * SDS fr a chemical r standard slutin (see nte belw). This mainly applies t vendr-prepared standards which cntain a multitude f cmpunds. (2) Reprductive txin: a chemical cnfirmed as a reprductive txin in the SDS fr the chemical (see nte belw). OSHA regulatins ( ) define reprductive txins as chemicals which affect the reprductive capabilities including chrmsmal damage (mutatins) and effects n fetuses (teratgenesis). (3) Cmpund exhibiting high acute txicity: a chemical fr which the txicity data frm SDS fr chemical (see nte belw) fall within OSHA s criteria fr high txicity. Accrding t OSHA, these cmpunds may be fatal r cause damage t target rgans after a single expsure r repeated expsures f shrt duratin. OSHA regulatins ( Appendix A) define a chemical as highly txic if it falls int ne f the fllwing categries: Oral: median lethal dse (LD50) 50 milligrams per kilgram f bdy weight, as administered rally t albin rats. Skin cntact: LD milligrams per kilgram f bdy weight, as administered by cntinuus cntact fr 24 hurs with the bare skin f albin rabbits. Inhalatin: median lethal cncentratin (LC50) in air f 200 parts per millin by vlume f gas r vapr, r 2 milligrams per liter f mist, fume, r dust, when administered by cntinuus inhalatin fr ne hur t albin rats. Nte that fr SDS frms, infrmatin regarding carcingenic effects, reprductive txicity, and acute txicity will generally be lcated under sectins titled Hazards Identificatin (ptential chrnic health effects) and/r Txiclgical Infrmatin. In the Labratry: A table listing the chemicals and slutins used in the WSS Chemistry Labratry that have been identified as Particularly Hazardus Substances is prvided in the Frms Appendix Expsure Mnitring In areas f regular use, the expsure f emplyees t Particularly Hazardus Substances fr which inhalatin is a significant expsure rute shuld be mnitred peridically. Expsure mnitring shuld assess the level f expsure at r near an emplyee s breathing zne using an rganic vapr mnitr badge (r similar device). Expsure limits fr air cntaminants are published as Permissible Expsure Limits (PEL s), which are generally based n 8-hur wrk shifts. Air expsure limits are lcated in OSHA regulatins (29CFR , Subpart Z, Tables Z-1 and Z2) and in SDS frms. Every effrt is made t ensure that emplyees are nt expsed t ambient air cncentratins abve the limits established by these regulatins. If expsure mnitring f an emplyees breathing zne indicates that expsure levels reach r exceed the limits fr a PHS, then mre rigid cntrl measures shuld be instituted t include: Additinal engineering cntrls Administrative cntrls such as limiting the expsure time Use f apprpriate persnal prtective equipment. Effective Date: Octber 1, f 66 Revised:

39 DWR-Labratry Safety 22.2 Wrking With a Particularly Hazardus Substance The key t safely wrking in clse prximity t a particularly hazardus substance is limiting the amunt f direct expsure an emplyee may have t the chemical r substance. General rules fr wrking with these substances include: 1. If pssible, wrk with a particularly hazardus substance under a fume hd. Verify that the fume hd is perating prperly BEFORE beginning wrk with the substance. 2. Persnal prtective equipment that is apprpriate fr the substance must be in use whenever the substance is handled. At a minimum, safety glasses, glves, and a lab cat must be wrn while wrking with a PHS. 3. Cmmunicate the hazards and emergency respnse prcedures t all persns in the area BEFORE wrking with the substance. 4. Wrking with a particularly hazardus substance requires that at least ne ther emplyee is present in the wrk area. The ther emplyee shuld be aware and knwledgeable f the prcedure t be used, the ptential dangers f the chemical r substance, and the emergency respnse activities in case f a spill r accident (decntaminatin prcedures, waste accumulatin and dispsal prcedures). 5. Infrmatin fr a particularly hazardus substance shuld be included in the Standard Operating Prcedure (SOP) r ther written instructins fr the prcedure(s). Infrmatin shuld include the hazard presented by the chemical, ptential expsure rutes, any special prtective equipment beynd the standard persnal prtective equipment, and emergency prcedures fr accidental spills f the chemical. 23 CHEMICAL WASTE MANAGEMENT Waste is cnsidered hazardus if it has ne r mre f the fllwing characteristics: * Ignitability: flammable r easily cmbustible with flashpint belw 140 F (e.g. rganic slvents). * Crrsivity: acids r bases, ph less than 2 r greater than 12.5; capable f crrding metal cntainers. * Reactivity: unstable at nrmal temperature and pressure; can cause explsin, release txic fumes, vaprs, r gasses when heated, cmpressed, r mixed with water. * Txicity: harmful r fatal when ingested r absrbed (e.g., mercury). Many chemicals used in the WSS Chemistry Labratry fall within the abve parameters. Therefre, whenever wastes are generated frm chemicals that exhibit the characteristics listed abve, they are t be treated as hazardus wastes. Slvent Waste Drum: Organic slvent waste (flammable) is cllected in a 55-galln steel drum (interir lined with plyethylene) that is lcated in the slvent strage rm (rm G102). The waste drum must be labeled as cntaining hazardus waste that is flammable and with the start date f additin f slvent waste t the drum. Only rganic waste may be stred in the drum; n water, acids r bases shuld be added. Transfer f slvent waste frm individual cllectin bttles (frm lab units) t the waste drum shuld nly be perfrmed by persnnel wearing the prper prtective equipment (slvent-resistant glves, respiratr, and face shield). Transfer int the drum must be dne with a static-free funnel attached t ne pening f the drum; the secnd drum pening shuld be partially pened fr ventilatin. When slvent waste is added t the waste drum, the fllwing infrmatin must be entered n an Organic Slvent Waste Frm (see Appendix) that is maintained fr the drum: date, chemist, name f slvent, and Effective Date: Octber 1, f 66 Revised:

40 DWR-Labratry Safety vlume f slvent (liters). The slvent waste drum shuld be inspected n a weekly basis and each inspectin dcumented n the Slvent Waste Drums Weekly Inspectin Lg (see Appendix). Slvent waste drums that are full must nly be remved frm the labratry by a hazardus waste cntractr. Hazardus waste manifests are t be cmpleted whenever slvent wastes are remved frm the labratry building. Neutralizatin Tank: The WSS Chemistry Labratry has a tw-stage sewage system. Labratry drains in the lab units are separated frm the sanitary drains (bathrms and break rm). The labratry sink drains are plumbed t cmbine waste water frm all f the lab unit sinks and transprt the water t a neutralizatin tank. The neutralizatin tank has a 1200 galln capacity and cntains 9000 punds f limestne pellets (calcium carbnate); limestne fr the tank shuld be replaced every 3 t 5 years, r when there is any indicatin that the limestne is n lnger prperly neutralizing the lab waste water. The limestne acts t neutralize the waste water (which can cntain acidic and alkaline slutins) and the neutralized water is then passed int the nrmal sanitary sewer system. The sanitary drains bypass the pretreatment phase and drain directly t the sanitary sewer system. Additinal guidelines regarding hazardus waste and chemical waste management: 1. Each labratry unit must maintain (if needed) clearly labeled, clsed cntainers fr the cllectin f hazardus chemical wastes. 2. All receptacles fr hazardus waste shuld be resistant t the materials and shuld be placed in a chemically resistant tray f sufficient vlume t cntain a rupture f the primary cntainer. 3. Liquid wastes are t be stred in screw-capped bttles r safety cans. Maintain a headspace avid cmpletely filling waste cntainers. 4. Chemical Waste shuld be segregated int slids and liquids. Liquids shuld be segregated int acids, bases, rganic slvents, r ther classificatin. Slid waste shuld be segregated by hazard class. 5. Certain chemical wastes (e.g., heavy metals, cyanides, carcingens, cntaminated prtective clthing) shuld be accumulated in separate cntainers t avid chemical reactins and t assist in classifying waste fr dispsal. 6. Cntainers f expired r unused hazardus chemicals r reagents frm lab units shuld be transferred t the acid and slvent waste strage rms (G102 and G106) fr temprary strage. Waste and expired chemicals shuld nly be remved frm the lab by a hazardus waste cntractr. A transprtatin manifest must be cmpleted (and kept n file) any time hazardus chemicals are remved frm the lab. 7. On an annual basis, the WSS Chemistry Labratry shuld evaluate the hazardus waste reductin prgram as per the Hazardus Slid Waste Amendments t the Resurce Cnservatin and Recvery Act. 24 BIOLOGICAL WASTE MANAGEMENT Bilgical wastes are t be handled as fllws: 1. Petri dishes used fr plating ut clny cultures are t be placed in an autclavable bihazard bag and sterilized prir t dispsal. 2. Cntainers and strage areas must be labeled prperly, and are t be clearly marked as having ptential bilgical cntaminants. Effective Date: Octber 1, f 66 Revised:

41 DWR-Labratry Safety FORMS APPENDIX Effective Date: Octber 1, f 66 Revised:

42 DWR-Labratry Safety Certificatin f Unit Safety Training - Labratry Water Sciences Sectin - Divisin f Water Resurces Emplyee Name: Labratry Unit: Supervisr Name: Designated Trainer: With my signature belw, I acknwledge that I have been instructed by my unit supervisr (r designated trainer) n the health and safety hazards present in my current wrk area(s) (list rm numbers: ) and the prper safety prcedures t fllw when wrking in these areas. The hazards and prcedures are utlined in the Labratry Sectin, as well as Standard Operating Prcedures fr the lab unit. I understand these hazards and accept them as a necessary part f my wrk. I will fllw the prper safety prcedures in my wrk area at all times. Emplyee Signature Date Supervisr Signature Date M:/LABFORMS/TRF (COU) Revised: 5/2014 Effective Date: Octber 1, f 66 Revised:

43 DWR-Labratry Safety New Emplyee Safety Orientatin & Training Chemistry Labratry - Water Sciences Sectin - N.C. Divisin f Water Resurces Date f Orientatin: Name f Emplyee: Orientatin Instructr: Safety Overview Recgnizing Wrk-Area Hazards Safety Devices available in Labratry and Unit Reprting Accidents and Injuries First Aid Kits Fire Preventin Guidelines Husekeeping Rules, Clthing, Washing Lab Cats Persnal Prtective Equipment Lcatin Instructins fr Use Additinal Prtective Equipment used in Unit Evacuatin Plan When t Evacuate Building and Where t G Alarm System General Labratry Hazards Equipment Hazards Electrical Hazards Cmpressed Gas Cylinders Autclaves Vacuums Nise Expsure Fume Hds When and Hw t Use a Fume Hd Alarm Chemicals Overview f Chemicals used in the Labratry Hazardus Chemicals used in Unit Material Safety Date Sheets Strage, Cmpatibility, Spill Respnse Transprting Chemicals Dispsal f Hazardus and Txic Chemicals Other Safety Issues fr Unit By signing belw, the emplyee and instructr verify that the abve items were discussed and understd. Signature f Emplyee Date Signature f Instructr Date M:/LABFORMS/TRF (NES) Revised: 5/2014 Effective Date: Octber 1, f 66 Revised:

44 DWR-Labratry Safety New Emplyee Orientatin and Training Date f Orientatin: Name f Emplyee (and signature): Persn Perfrming Orientatin (and signature): Safety Plan Review Emergency Actins and Ntificatin Reprting Labratry Accidents Fire Preventin Guidelines Husekeeping Rules, Clthing and Persnal Items Recgnizing wrk area hazards Persnal Prtective Equipment Lcatin Instructins fr Use Evacuatin Rutes When t Evacuate Alarm System General Labratry Hazards Hazardus Equipment Nn-hazardus Equipment Electrical Hazards Cmpressed Gases Vacuums Nise Expsure Fume Hd Use When ne shuld use the Fume Hd Operatin f the Fume Hd Chemicals used in the Labratry Inventry f Chemicals used in the Labratry Hazard Classes f Chemicals used in the Labratry Material Safety Data Sheets Strage Extremely Hazardus and Txic Materials Transprting Chemicals Hazardus Waste Dispsal M:/LABFORMS/TRF-002-1(CHP) rev. 5/04 Effective Date: Octber 1, f 66 Revised:

45 DWR-Labratry Safety Effective Date: Octber 1, f 66 Revised:

46 DWR-Labratry Safety Fire Drill Checklist - DWR Water Sciences Sectin Lcatin f Fire Drill: 4405 Reedy Creek Rad, Raleigh, NC Date f Fire Drill: Persn(s) Cnducting Fire Drill: Persn that Activated Fire Alarm: State Capitl Plice Ntified? YES NO Time when Fire Alarm was Activated: AM / PM All emplyees accunted fr? Vlatiles Unit: YES NO SV/Pesticides Unit: Bichemistry Unit: Micrbilgy/Metals Unit: Frnt ffice / Receiving Area: YES YES YES YES NO NO NO NO Elapsed Time t Evacuate Building: Fire Alarm Heard Clearly in all Areas? YES NO Drs t Lab Units Clsed? Crridrs and Exits Clear f Obstructins? YES YES NO NO Weather Cnditins at Time f Drill: Cmments and Ntes: Revised: 7/2014 Effective Date: Octber 1, f 66 Revised:

47 DWR-Labratry Safety Fume Hd Infrmatin: Make/Mdel, Sash Opening Dimensins, Air Flw Measurement Fume Hd Make -lcatins- Hamiltn - large -G066 (Wet Chem.) -G043 (Extractins) Hamiltn - large -G091 (Micr) -G066 (TDS) Hamiltn - medium -G031 (TKN) -G066C (Chlrphyll) Hamiltn - small -G047 (Pesticides) -G065 (Vlatiles) TFI large -G048 & G066 -G113 (Metals) Hd Sash Opening: Base Width (inches) Vertical Height (inches) Hrizntal Measurement Psitins: Number f Psitins Psitin 1 -frm left side- (inches) Distance between Psitins (inches) ½ ½ ½ (Pest) 22 (Vl.) TFI medium -G113 (Metals) Effective Date: Octber 1, f 66 Revised:

48 DWR-Labratry Safety Fume Hd Evaluatin Wrksheet NCDWR-Water Sciences Sectin, 4405 Reedy Creek Rad, Raleigh, NC Hd ID: Lab Unit: Rm Number: Date f Evaluatin: Initials: Lab Dr(s) Clsed? Yes / N (Circle One) Hd Baffle: Open Psitin the Hd Sash at Maximum Certified Height - Hd Opening Measurements: Height (inches): Hd Mnitr Display: Width (inches): ( ) Nrmal ( ) Alarm Light Flashing ( ) Other: Fume Hd Face-Velcity Measurements (feet per minute, fpm) Vertical Psitin 1 (belw hd sash) Height (inches) Hrizntal Psitin Average A B C D E F fpm 2 (mid-pint) 3 (base f hd) Distance frm Left Side (in.): Distance between Psitins (in.): Cmments: Date f Evaluatin: Initials: Lab Dr(s) Clsed? Yes / N (Circle One) Hd Baffle: Open Psitin the Hd Sash at Maximum Certified Height - Hd Opening Measurements: Height (inches): Hd Mnitr Display: Width (inches): ( ) Nrmal ( ) Alarm Light Flashing ( ) Other: Fume Hd Face-Velcity Measurements (feet per minute, fpm) Vertical Psitin 1 (belw hd sash) Height (inches) Hrizntal Psitin Average A B C D E F fpm 2 (mid-pint) 3 (base f hd) Distance frm Left Side (in.): Distance between Psitins (in.): Cmments: Effective Date: Octber 1, f 66 Revised:

49 DWR-Labratry Safety ORGANIC SOLVENT WASTE LOG SOLVENT WASTE DRUM ID: NOTE: Organic Slvents Only - DO NOT ADD Acid, Bases, r Water Date Organic Slvent Vlume (Liters) Initials Cmments: Effective Date: Octber 1, f 66 Revised:

50 DWR-Labratry Safety Slvent Waste Drums - Weekly Inspectin Lg (lcated in Rm G102) Drums Drum Integrity Drums Grunding Adequate Date Initials Clsed? N Leaks? Labeled? Wires Secure? Clearance/Space? Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Yes / N Cmments. Prblems, Crrectins: Effective Date: Octber 1, f 66 Revised:

51 DWR-Labratry Safety Effective Date: Octber 1, f 66 Revised:

52 DWR-Labratry Safety Chemical Benzene List f Particularly Hazardus Substances: Water Sciences Sectin, 4405 Reedy Creek Rad Chemical Frm Cmpnent f VOC mixture Lcatin in Labratry Carcingens Vlatiles Surce f Hazard Designatin OSHA 1, NTP 2, IARC (1) 3 : knwn carcingen Beryllium Cmpnent f standard mixture Metals NTP, IARC (1): knwn carcingen Cadmium Slid; cmpnent f standard mixture Nutrients; Metals OSHA, NTP, IARC (1): knwn carcingen Chlrfrm Liquid Wet Chemistry NTP, IARC (2B): suspect carcingen Chrmic acid Cmpnent f digestin slutin Wet Chemistry MSDS (cntains chrmium (VI)) Chrmium (VI) Cmpnent f standard mixture Metals OSHA, NTP, IARC (1): knwn carcingen Frmaldehyde Liquid Wet Chemistry OSHA, NTP, IARC (1): knwn carcingen Lead cmpunds Cmpnent f standard mixture; lead acetate Metals; Wet Chemistry NTP, IARC (2A): suspect carcingen Methylene chlride Liquid slvent SV/PE 4 OSHA, NTP, IARC (2B): suspect carcingen Phenlphthalein 0.5% Slutin Wet Chemistry NTP, IARC (2B), MSDS: suspect carcingen Ptassium dichrmate Slid Wet Chemistry OSHA, NTP, IARC (1): knwn carcingen Standards fr SV/PE 5 SV/PE MSDS's fr standards Standards fr Vlatiles 5 Vendr-prepared standard mixtures Vlatiles MSDS's fr standards Vinyl Chlride Cmpnent f VOC Gas mixture Vlatiles OSHA, NTP, IARC (1): knwn carcingen Ether Vendr-prepared standard mixtures Liquid Reprductive Txins n-hexane Liquid Wet Chemistry MSDS SV/PE MSDS Lithium Cmpnent f standard mixture Metals MSDS Mercury Cmpnent f standard mixture, thermmeters Metals MSDS Ptassium dichrmate Slid Wet Chemistry MSDS Pyridine Liquid Wet Chemistry MSDS Sdium Tetrabrate Slid Nutrients MSDS Tluene Liquid Organics MSDS Substances with High Acute Txicity Phenl Liquid Nutrients MSDS Ptassium cyanide Cmpnent f standard slutin Wet Chemistry MSDS Ptassium dichrmate Slid Wet Chemistry MSDS (dermal and inhalatin) Standards fr SV/PE Vendr-prepared standard mixtures SV/PE MSDS's fr standards 1 OSHA = Occupatinal Safety and Health Administratin; 2 NTP = Natinal Txiclgy Prgram 3 IARC = Internatinal Agency fr Research n Cancer (carcingen gruping: 1 = knwn; 2A = prbable; 2B = pssible) 4 SV/PE = Semi-vlatiles and Pesticides 5 Vendr-prepared standards fr the SV/PE and vlatiles units cntain cmpnents that are carcingenic; please refer t MSDS's fr mre infrmatin. Date Prepared: 7/2014 Effective Date: Octber 1, f 66 Revised:

53 DWR-Labratry Safety APPENDIX A Labratry Chemical Spill Plan Effective Date: Octber 1, f 66 Revised:

54 DWR-Labratry Safety 1. Emergency Assistance NC Department f Envirnmental Quality Divisin f Water Resurces - Central Labratry Labratry Chemical Spill Plan 9/2015 Call 9911 (City f Raleigh Emergency Respnse) t assist with the fllwing: medical assistance fr injuries fires explsins chemical spills fr which assistance is required Emergency Respnse persnnel will dispatch apprpriate respnse persnnel. When yu call, yu shuld be ready t prvide the fllwing infrmatin: 2. Spill Preparedness What is the name f the chemical spilled? What quantity f the chemical has spilled? Where is the spill (building lcatin and flr number)? Has anyne cme in cntact with the chemical? Is anyne injured? Is a fire r explsin invlved? What is yur name and phne number? Mst labratry spills and many small chemical spills utside the labratry wrk area can be safely handled by labratry persnnel. Sme spills shuld nly be mitigated by specially trained emergency respnse persnnel. It is prudent t make preparatins fr dealing with spills befre they ccur. This sectin prvides basic emergency preparedness infrmatin and gives general guidance n hw t respnd t chemical spills. A. Preventing spills: Listed belw are sme basic spill preventin steps that apply t strage, transprtatin, and transfer f chemicals. General precautins: reduce clutter and unnecessary materials in yur wrk area eliminate tripping hazards and ther bstructins have all needed equipment readily available befre starting wrk take yur time dn t rush thrugh yur task Strage precautins: use sturdy shelves d nt stre abve eye level stre larger cntainers clser t the flr cntainers shuld be stred at the rear f shelves strage shelves shuld have raised edges ( lips ) stre chemicals first by cmpatibility, then alphabetically inspect the strage area regularly fr leaking r defective cntainers use strage cntainers apprpriate fr the stred materials Effective Date: Octber 1, f 66 Revised:

55 DWR-Labratry Safety d nt stre unprtected glass cntainers n the flr use spill prf trays r bttle carriers in acid cabinets Transprtatin precautins: use high-walled carts, where apprpriate use safety cntainers, where apprpriate use bttle carriers r a cart fr 2.5 and 4.0 liter bttles use straps t secure cntainers, where apprpriate cnsider ptential hazards and escape rutes befre transprting chemicals cnsider purchasing plastic cated "shatter resistant" bttles Precautins in transferring chemicals: B. Preparing fr spills pay careful attentin t the size f cntainer t avid verfilling transfer nly the quantity needed if pssible, use pumps r ther mechanical devices rather than puring prvide cntainment t capture leaks and spills Befre wrking with chemicals yu shuld determine what may g wrng and hw yu will respnd t a spill. Organize any spill respnse by establishing prtcls and evaluating ptential hazards in advance. Becme familiar with Safety Data Sheets fr cmmnly used hazardus chemicals, and review SDSs prir t using an unfamiliar material. Make sure that yu have all necessary persnal prtective devices and safety equipment. Ensure that prper cntainment and clean up materials are readily available and easily accessible. Each individual wh may be invlved in spill respnse must knw the lcatin, purpse and limitatins f all persnal prtective equipment, safety equipment and clean up materials. Spill cntrl kits: Spill cntrl materials have been made available and are lcated in the labratry wrk areas. Prepackaged kits are als available. Kits will include several items that may be needed fr handling a chemical spill such as: Dispsable, chemical resistant glves Safety gggles Absrbent pillws and/r pads Plastic bags fr cntaining absrbent material Neutralizing agents Plastic pails The spill cntrl kits are clearly marked. All lab persnnel shuld knw their lcatin, be familiar with their cntents, and understand their limitatins. C. Defining and classifying a spill There are tw basic types f spills: mercury spills and chemical spills. Mercury spills require assistance fr safe and prper cllectin. A mercury spill kit is available and lcated in the labratry stck rm. Request assistance frm labratry supervisrs r thers trained in the use f the spill kit. Chemical spills can be brken dwn int tw basic types: simple spills, which yu can clean up yurself, and cmplicated spills, which require assistance. Effective Date: Octber 1, f 66 Revised:

56 DWR-Labratry Safety If the spill meets ANY f the fllwing cnditins f a cmplicated spill, call 9911 immediately. A spill is cmplicated if: HANDLING CHEMICAL SPILLS A. Evaluate hazards a persn is injured identity f the chemical is unknwn multiple chemicals are invlved the chemical is highly txic, flammable r reactive the spill has the ptential t spread t ther parts f the building (such as thrugh the ventilatin system) the cleanup prcedures are nt knwn r apprpriate materials are nt readily available the spill may endanger the envirnment (such as reaching waterways r utside grund) When spills ccur, a quick and apprpriate respnse can prevent serius cnsequences. Imprper respnse can make things wrse. In rder t respnd prmptly and apprpriately, yu shuld evaluate ptential hazards befre using any chemicals. The first surce f infrmatin t cnsult shuld be the Safety Data Sheets (SDS). SDSs may be fund in white ntebks lcated in the frnt ffice area f the Chemistry Labratry Building. Of greatest cncern in spill situatins are chemicals that are: air reactive water reactive flammable plymerizable crrsive txic Based n these hazards, yu can then determine: apprpriate persnal prtective equipment fr spill respnse types f fire suppressin equipment apprpriate cleanup materials first aid prcedures B. Specific spill respnse measures fr simple spills Ntify labratry supervisrs and cwrkers f the spill. Prevent the spread f fumes and vaprs. If the substance is vlatile r can prduce airbrne dusts, clse labratry drs t islate the area. Cntrl the spread f liquids. Cntain the spill by using absrbent material such as Spill-X, Oil- Dri, cat litter, vermiculite, r spill pillws. Place absrbents r spill pillws at the uter edges f the spill. (D nt use silica [sand] based prducts with hydrfluric acid.) Neutralize acids and bases. Spills f acids r bases shuld be neutralized unless txic fumes are present. Exercise cautin in neutralizing spills as the reactin can be vigrus, causing spattering and heat. Absrb liquids. Add the absrbents t the spill, wrking frm the uter edges tward the center. Absrbent materials such as Spill-X, Oil-Dri, cat litter, r vermiculite wrk well, but can be difficult t clean up. Spill pillws are easier t use and clean up. Effective Date: Octber 1, f 66 Revised:

57 DWR-Labratry Safety Cllect and cntain the cleanup residues. Spill residues and cleanup materials shuld be cllected and placed int a plastic cntainer. If the residues are ttally dry, they may be placed in a plastic bag. Place a descriptive label n any residue cntainer. Decntaminate the area and affected equipment. Ventilate the area if necessary. Fr mst hard surfaces, cnventinal cleaning with sap and water is apprpriate. Dispse f the wastes. Labratry supervisrs will prvide guidance fr dispsal f wastes. C. Specific spill respnse measures fr cmplicated spills Ntify the safety fficer, supervisrs, and cwrkers. Evacuate the area. Leave the spill area; alert thers in the area. Withut endangering yurself: remve victims t fresh air, remve cntaminated clthing, and flush cntaminated skin with water fr 15 minutes. If anyne has been injured call 9911 immediately. It may be necessary t evacuate the building fr extremely txic spills. Cnfine the spill area. When everyne is safely ut f the area, clse drs and islate the spill area. If the spill invlves a flammable material, disable electrical supply using the red buttns utside the labratry area. Reprt the spill. Frm a safe lcatin, call Reprt that has been a chemical spill at the DEQ Chemistry Labratry / 4405 Reedy Creek Rad, and give the name f the chemical, the quantity spilled, spill lcatin (building lcatin), the extent f any injuries, whether a fire r explsin is invlved, and yur name and phne number. Secure the area. Until emergency respnders arrive, blck ff areas leading t the spill, lck drs, and pst signs r warning tape t alert thers f the spill. Pst staff by cmmnly used entrances t the area in rder t direct peple tward ther rutes. Arrange fr smene t meet the emergency respnders at the main entrance t the cmplex. Clean up the spill. After emergency respnders have cntrlled the spill, cnsult with the safety fficer r supervisrs t determine ther needed cleaning prcedures. In sme cases this may be dne by labratry persnnel; in ther cases, an utside cntractr may be required. D. CLEANING UP HAZARDOUS MATERIALS SPILLS 1) Slightly Hazardus Materials (Weak Acids, Bases, Oils, etc.) Absrb using paper twels, Spill-X-A fr acids / Spill-X-C fr caustics, and/r spill-cntrl pillws. Refer t Appendix B fr Spill-X prcedures. Transfer t a plastic bag, place in trash cntainer fr slid wastes. Clean flr (r ther spill surface) thrughly with sap and water; dry surface cmpletely t prevent slips r falls. 2) Mercury Spill Cntact labratry supervisrs t perfrm the cleanup Cntain the spill in a small area and warn c-wrkers nt t enter the area. Use glves; reduce any surces f heat that might vlatilize the mercury. D nt use an rdinary vacuum cleaner; it will aerslize and spread the mercury thrugh the labratry. If the mercury has reached a prus surface (flr, lab bench, etc.), special chemical inactivatrs must be used. The mercury spill kit is lcated in the labratry stck rm. Refer t Appendix C fr the mercury spill kit prcedure. Several cleanings may be necessary t remve all f the mercury. 3) Cncentrated Acids/Bases Effective Date: Octber 1, f 66 Revised:

58 DWR-Labratry Safety Wear heavy glves that are acid resistant, gggles, and bdy prtectin (lab cat and rubber aprn). Take care nt t step in the spilled materials. Absrb the spill using acid r base Spill-X and/r absrbent pillws. DO NOT USE PAPER PRODUCTS. Prducts designed t neutralize acids r bases can prduce large amunts f heat and may splatter. Treat the spill in small sectins. Verify the material is neutralized by checking ph (see Spill-X prcedure.) Carefully place the neutralized materials int bags and prperly dispse. Clean the area thrughly with sap and water; ensure that the area is dry t prevent slipping. 4) Flammable/Vlatile Chemicals If pssible, immediately extinguish all pen flames. Shut dwn all surces f ignitin (sparking equipment, pen flames, etc.). This can be dne using the electric supply cutffs (red buttns) in the hallways utside each labratry. Remember, fr explsive gases, the spark frm turning n a light switch can be sufficient t ignite the gas. Vaprs can migrate frm pen cntainers r spills. Open cntainers f flammable materials shuld be islated within a fume hd r apprpriate strage cabinet during spill cleanup. Alert ther wrkers present in the lab and seek assistance if necessary. Use glves t prtect yur skin. Ensure that yu are using the prper glve material (see Appendix D fr a glve cmpatibility guide.) Absrb the spill using spill-cntrl pillws and/r Spill-X-S adsrbent materials designed fr vlatile agents. Place the saturated pillw r absrbent materials int a hazardus waste bag and place the bag int a fume hd r utside the main labratry, if pssible. Use a shvel, scp r brm t remve the material. All utensils cming in cntact with the spilled substance must be cleaned r discarded. Clean up the cntaminated area using sap and water. E. Special Precautins 1) Special precautins fr flammable liquids Remve all ptential surces f ignitin, when pssible. If, hwever, the vaprs frm a flammable spill are in the vicinity f an ignitin surce it may be advisable t shut ff pwer using the electrical system cutffs (red buttns) utside each labratry area. Ntify supervisrs fr assistance. If respiratry prtectin is necessary, cntact Cntain spill cleanup materials in a sturdy plastic bag and label. Never use a vacuum cleaner t cllect flammable liquids. Thrughly ventilate the area when cleaning is cmpleted. 2) Special precautins fr acids Prtect skin and eyes frm direct cntact with the acid. Respiratry prtectin may be necessary; call 9911 if needed. Neutralize the spill with carbnate r bicarbnate f sdium r ptassium. The neutralizatin reactin is smetimes vigrus, s be alert fr splashes. Use ph paper t verify that the acid has been neutralized. Special precautins shuld be taken when cleaning up hydrfluric acid (HF) spills. D nt use silica [sand] based prducts with hydrfluric acid. Effective Date: Octber 1, f 66 Revised:

59 DWR-Labratry Safety 3) Special precautins fr pwder spills Befre starting, clse windws and drs t reduce drafts. Carefully sweep up the pwder t minimize dust. Place all residues in a sealed cntainer. If needed, use a dust mask r respiratr, as apprpriate. Yu must be certified t wear a respiratr. 4) Special precautins fr mercury spills Labratry supervisrs shuld assist with the cleanup. Crdn ff the area t prevent the mercury frm spreading. If yu break a mercury thermmeter, a mercury spill kit is available fr cleaning up the spill. Place any cntaminated items frm the kit, and the brken thermmeter, in a sturdy plastic bag. Clse and label the bag "Brken mercury thermmeter". D NOT use a regular vacuum cleaner r shp vac. Nte: The best methd fr dealing with mercury spills is t prevent them in the first place. Examine all uses f mercury t determine if substitutes are available. Stre devices cntaining mercury in trays r ther equipment that prvide cntainment in the event f a spill. Refer t Appendix C fr a mercury spill kit prcedure. 5. EXPOSURE TO CHEMICALS A. If a chemical cmes in cntact with skin, wash the area immediately using warm water and a strng sap such as dishwashing detergent. D nt use alchl, acetne, r ther slvents (these will facilitate absrptin f the chemical thrugh the skin). B. Splashes int eyes, nse, r lip area must be remved immediately using an eyewash statin/hse r by placing the area under a steady stream f lukewarm running water. A mild sap may be used fr the lips and nse, but nly running water is t be used fr the eyes. It is imprtant that the eyes be held pen in the stream f water and that cntact lenses are remved t facilitate cleansing f the eyes. Rll the affected eye during rinsing, which shuld cntinue fr at least 15 minutes. Seek medical attentin immediately. C. If a chemical has been spilled nt clthing r ther parts f the bdy, remve any cntaminated clthing r jewelry immediately and wash the affected area with warm water and sap fr at least 10 minutes. Cntact with phenl, slutins cntaining dimethyl sulfxide (DMSO), cyanate r pyridine can be especially serius, since these cmpunds pass thrugh the skin and int the bldstream rapidly. Dispse f cntaminated clthing in the same manner as chemical wastes. D nt reuse clthing and lab cats cntaminated with a hazardus substance. Seek medical attentin. D. If a txic substance is inhaled, the substance is absrbed rapidly int the bldstream. Persnnel expsed in such a manner shuld leave the cntaminated area and seek immediate medical attentin. Attempt t cugh up as much mucus as pssible and rinse muth with water. Qualified persnnel shuld remve incapacitated r uncnscius victims frm the cntaminated area nly with the use f SCBA (self-cntained breathing apparatus). Never attempt t remve a victim frm a rm cntaining txic vaprs withut using a prtective breathing apparatus. Be cautius abut using CPR n a persn wh has inhaled txic vaprs in rder t prevent crss-cntaminatin. Place warning signs at the rm entrance where the accident ccurred t indicate cntaminated area. Emplyees expsed t txic substances shuld be referred t medical persnnel fr further evaluatin. E. If materials have been ingested, rinse muth with warm water repeatedly. If crrsives were ingested, d nt attempt t neutralize the chemical by any means unless directed t d s by medical persnnel. D nt attempt t induce vmiting if crrsives have been swallwed since this may damage the esphagus. If ther txic substances have been swallwed, water may be used t dilute the txin. Vmiting may be induced nly if crrsives r hydrcarbns (petrleum distillates) are nt invlved. Seek medical attentin and be prepared t infrm the medical authrities abut the nature and apprximate amunt f chemical that was ingested. Effective Date: Octber 1, f 66 Revised:

60 DWR-Labratry Safety Appendix B Spill-X Agents Standard Operating Prcedure Effective Date: Octber 1, f 66 Revised:

61 DWR-Labratry Safety 1.0 Purpse 1.1 T instruct labratry persnnel n prper use f Spill-X Agents n acid, base r slvent spills. 2.0 Assciated Equipment 2.1 Spill-X A / Spill-X C / Spill-X S 3.0 Assciated Reference Material 3.1 Spill-X Instructins 4.0 Sample Handling 4.1 See Spill-X bttle, rear label 5.0 Interferences 5.1 N/A 6.0 Chemicals & Reagents 6.1 Spill-X A fr acids 6.2 Spill-X C fr caustics / bases 6.3 Spill-X S fr slvents 7.0 Prcedure ENSURE THAT THE SPILL AREA HAS BEEN ISOLATED. PERSONAL PROTECTIVE EQUIPMENT SUITABLE FOR THE REAGENT SHOULD BE WORN. IF A FUMING ACID IS SPILLED, ENSURE THAT ADEQUATE AIRFLOW IS PRESENT BEFORE CONTINUING. (Cnsult all applicable Safety Data Sheets, including thse fr Spill-X.) 7.1 Identify the reagent. There are different Spill-X agents fr acids, bases, and slvents. SPILL-X-A fr ACID SPILL-X-C fr CAUSTIC (bases) SPILL-X-S fr SOLVENT 7.2 Surrund the spill with the apprpriate Spill-X prduct and then begin puring directly nt the spill area. Avid splashing as Spill-X agent is applied. 7.3 Mix the Spill-X reagent int the spilled material with a plastic scraper and allw reactin t take place. CAUTION: The reactin f the Spill-X reagent with the spilled material may create heat and strng drs. 7.4 ph Test Prcedure: Spill-X-A and Spill-X-C absrb target liquids, prducing a paste-like residue. After it cls, cllect the residue and place int a suitable cntainer. Test the ph f the residue as fllws t ensure that cmplete neutralizatin has ccurred: Place 10 cc f the residue int a 150 ml beaker Effective Date: Octber 1, f 66 Revised:

62 DWR-Labratry Safety Slwly add distilled water t a vlume f 100 ml. Stir fr a few minutes. NOTE: If faming r heating ccurs, neutralizatin has nt been achieved and mre Spill-X must be added Use ph test strips t test the slutin. A ph f must be achieved in rder t classify the residue as nn-hazardus waste per EPA guidelines Recrd the final ph n the dispsal cntainer. Accrding t the substance absrbed (chrmic acid, etc.), the residue may be classified as hazardus waste. Dispse f the cntainer prperly. 7.5 Spill-X-S wrks by adsrbing the slvent nt a carbnaceus matrix leaving a dry, pwdery substance. The residue can be cllected and placed int a plastic Ziplc bag fr dispsal. 7.6 Table 1 indicates the amunt f a given reagent that can be neutralized by ne cntainer f the apprpriate Spill-X agent (see belw): Table 1: ACID SPILLS Spill-X-A (2.5 lb cntainer) ACID CONCENTRATION AMT NEUTRALIZED (L) Acetic 99% 1.14 Frmic 90% 0.93 Hydrchlric 37% 1.00 Nitric 70% 2.08 Phsphric 85% 1.14 Sulfuric 93% 1.08 Other Acids Apprx. 1 L CAUSTIC SPILLS Spill-X-C (2.0 lb cntainer) CAUSTIC CONCENTRATION AMT NEUTRALIZED (L) Ammnium Hydrxide 29% 1.32 Ptassium Hydrxide 45% 0.87 Sdium Hydrxide 50% 0.54 Other Bases Apprx. 0.5 L SOLVENT SPILLS Spill-X-S (1.0 lb cntainer) FLAMMABLE SOLVENTS AMT NEUTRALIZED (L) 1,1,2-Trichlrethane 0.91 Acetne 0.76 Ethanl 0.45 Hexane 0.45 Isprpyl Alchl 0.68 Methanl 0.45 Other Slvents Apprx. 0.5 L Other reagents are listed in the Spill-X Spill Kit Treatment Guide. Effective Date: Octber 1, f 66 Revised:

63 DWR-Labratry Safety Appendix C Mercury Spill Cleanup using the Mercn Spill Kit Standard Operating Prcedure Effective Date: Octber 1, f 66 Revised:

64 DWR-Labratry Safety 1.0 Purpse 1.1 T instruct persnnel n safe and prper clean-up f a mercury spill using the Mercn spill kit. 2.0 Assciated Equipment 2.1 Mercn Mercury Spill Kit 3.0 Assciated Reference Material 3.1 Mercn Mercury Spill Kit Instructin Guide 4.0 Sample Handling 4.1 See Kit Cntents 5.0 Interferences 5.1 N/A 6.0 Chemicals & Reagents 6.1 See Kit Cntents 7.0 Prcedure 7.1 Check clthing & ftwear fr mercury cntaminatin. Remve any cntaminated apparel and place in a bihazard bag fr dispsal. 7.2 Islate the area f the spill and ensure that n ther persnnel enter the area. 7.3 Ntify a labratry supervisr f the spill. Prceed nly under supervisin f a labratry supervisr. 7.4 Ensure that the glves and gggles supplied with the kit are used. 7.5 Spray the Mercn spray int the ambient air, starting at eye level, and wrk dwn t the flr n the visible spill and surrunding area. 7.6 Use Mercn Vap slutin t cver any visible mercury beads. Cautin: The flr may becme slippery. 7.7 Use the mercury aspiratr t pick up mercury beads and depsit int the Mercn Tainer. When depsiting the beads, ensure that the tip f the aspiratr is belw the rim f the Mercn Tainer. 7.8 Wipe the cntaminated area with a ChemWipe saturated with Mercn Vap slutin. 7.9 Wipe the dispsable glves with anther Mercn Vap saturated ChemWipe Place the used ChemWipes and glves int a dispsal bag Place the dispsal bag int a sturdy cntainer and stre in a secure lcatin until ready fr dispsal Ensure that a replacement Mercury Spill Kit is btained as sn as pssible NOTE: Mercury is a hazardus waste and must be dispsed f accrding t lcal, state, and Federal laws. Effective Date: Octber 1, f 66 Revised: